Abstract:
:We have in recent studies presented unexpected immunohistochemical evidence favoring the existence of a local production of catecholamines, and an occurrence of adrenergic receptors on the tendon cells (tenocytes), in the human patellar tendon. This was particularly noticed for tendons from patients suffering from tendinosis (chronic tendon pain), which has led us to propose an involvement of this autocrine/paracrine system in the development of tendinosis, especially since catecholamines have been reported to be modulators of tissue remodeling and pain processes. However, the findings concerning catecholamine production have so far only been noted at the level of protein detection, and for this reason, the aim of the present study was to confirm the previous immunohistochemical results by using in situ hybridization (ISH) technique. A ssDNA probe detecting human mRNA for the catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) was applied. The ISH results revealed that there were clear reactions indicating the existence of mRNA for TH in tenocytes of tendinosis specimens. It was generally noted that disfigured tenocytes were the ones with the most distinct reactions, while normally looking tenocytes hardly displayed any reactions at all. In conclusion, this study presents the first evidence at the mRNA level of the existence of a local nonneuronal production of catecholamines in human patellar tendon tissue. The findings add to recent observations of the occurrence of a local production in tendons of signal substances traditionally related to neurons.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Danielson P,Alfredson H,Forsgren Sdoi
10.1002/jemt.20495subject
Has Abstractpub_date
2007-10-01 00:00:00pages
908-11issue
10eissn
1059-910Xissn
1097-0029journal_volume
70pub_type
杂志文章abstract::From the first cases of bovine spongiform encephalopathy (BSE) among cattle in the United Kingdom in 1986, the route of infection of BSE is generally believed by means of feeds containing low level of processed animal proteins (PAPs). Therefore, many feed bans and alternative and complementary techniques were resulted...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20951
更新日期:2011-08-01 00:00:00
abstract::Transgenic mice are valuable experimental models of human endocrine diseases. Targeted ablation of specific cell lineages or insertion of genes coding for releasing factors, hormones, growth factors, and oncogenes fused with appropriate promoters, or mutated genes, can induce several pituitary disorders. Various hypos...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19971015)39:2<194::AID-JEM
更新日期:1997-10-15 00:00:00
abstract::The ionic liquids (ILs) are recognized as the potential solvents for the pretreatment of lignocellulosic materials before biomass conversion. However, little knowledge of how the cell wall of biomass responds to the IL locally and dynamically during the pretreatment is available. In the current work, the process of IL...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22379
更新日期:2014-08-01 00:00:00
abstract::The family Scrophulariaceae consists of taxonomically complex genera and species. The delimitation of the taxa within this family is always challenging. In this paper, we studied leaf epidermis anatomical characteristics and its taxonomic significance of four species belonging to four genera of the family Scrophularia...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23608
更新日期:2020-09-29 00:00:00
abstract::In yeast, peroxisomes are the site of specific catabolic pathways that characteristically include hydrogen peroxide producing oxidases and catalase. During the last 10 years, much progress has been made in unravelling the molecular mechanisms involved in the biogenesis of this organelle. At present, 23 different genes...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20001215)51:6<584::AID-JEMT8>3.0
更新日期:2000-12-15 00:00:00
abstract::Laminins are alpha-beta-gamma heterotrimeric components of all basement membranes. Laminins are now known to play the central role in organizing and establishing the basement membrane. The diversity of laminins allows them to impart special structural and signaling properties to the basement membrane. Of the 12 known ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20563
更新日期:2008-05-01 00:00:00
abstract::The construction of the liver parenchyma throughout fetal development depends on the elaboration of intercellular contacts between epithelial cells and between epithelial and mesenchymal cells. During this time, the spatial distribution of cytokeratins in hepatocytes shows a striking evolution as demonstrated by confo...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19971201)39:5<436::AID-JEM
更新日期:1997-12-01 00:00:00
abstract::Atomic force microscope (AFM) has been widely used in the biological field owing to its high sensitivity (subnanonewton), high spatial resolution (nanometer), and adaptability to physiological environments. Nowadays, force volume (FV) and peakforce quantitative nanomechanical (QNM) are two distinct modes of AFM used i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23351
更新日期:2019-11-01 00:00:00
abstract::Transit from the nuclear complex to the cytoplasm through the nuclear pore complex permits modification of mRNA, including processing such as splicing, capping, and polyadenylation, etc. At each of these events, mRNA interacts with various proteins to form mRNA-protein complex. Visualizing the mRNA is crucial for unde...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23214
更新日期:2019-06-01 00:00:00
abstract::Research into the POU transcription factor Oct-6 has been the focus of much current attention, in particular its role in Schwann cell development and differentiation. Based on published data and data presented here, we propose a model for Oct-6 function at two distinct stages of Schwann cell maturation. First, Oct-6 f...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980601)41:5<372::AID-JEM
更新日期:1998-06-01 00:00:00
abstract::Integrated information on ultrastructural surface texture and chemistry increasingly plays a role in the biomedical sciences. Light microscopy provides access to biochemical data by the application of dyes. Ultrastructural representation of the surface structure of tissues, cells, or macromolecules can be obtained by ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20691
更新日期:2009-07-01 00:00:00
abstract::Investigation of mortuary ritual is an important method to reconstruct many aspects of past societies. Due to the lack of relevant analytical work, little evidence related to organic materials in a burial can be found in China. Here we report materials collected from a burial during the excavation of the Shengedaliang...
journal_title:Microscopy research and technique
pub_type: 历史文章,杂志文章
doi:10.1002/jemt.22791
更新日期:2017-02-01 00:00:00
abstract::The subcommissural organ (SCO) is a brain circumventricular organ formed by ependymal and hypendymal secretory cells. It secretes glycoproteins into the cerebrospinal fluid of the third ventricle where they condense into a thread-like structure known as Reissner's fiber (RF). The present study was designed to investig...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/1097-0029(20010301)52:5<496::AID-JEMT1035>
更新日期:2001-03-01 00:00:00
abstract::In animal oocytes, the centrosome exists as an acentriolar aggregate of centrosomal material that is regulated in a dynamic manner throughout the process of meiotic maturation. Recently, it has been demonstrated that in female meiotic systems spindle assembly is likely regulated by chromosomal and microtubule/microtub...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(20000601)49:5<435::AID-JEM
更新日期:2000-06-01 00:00:00
abstract::In order to test the effectiveness of a new advanced time-lapse microscopy imaging and image processing and analysis system, and to do quantitative and qualitative temporal analyses of in vitro peripheral nerve regeneration, long-term time-lapse imaging of cultures of mouse dorsal root ganglia (DRGs) was performed. DR...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20075
更新日期:2004-06-15 00:00:00
abstract::This was the first comprehensive pollen micromorphological investigation of lactiferous flora (Apocynaceae) of District Rawalpindi, Pakistan. The pollen morphology of 10 species of the family Apocynaceae was observed and documented using light microscopy and scanning electron microscopy. Pollen was found subspheroidal...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23293
更新日期:2019-09-01 00:00:00
abstract::Golgi impregnations reveal a variety of dendritic morphologies amongst Kenyon cells in the mushroom bodies of Drosophila melanogaster. Different morphological types of Kenyon cells contribute axon-like processes to five divisions of the medial and vertical lobes. Four of these divisions have characteristic affinities ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10368
更新日期:2003-10-01 00:00:00
abstract::A new and improved technique for microvascular corrosion casting was developed and verified by examination of corrosion casts of 90 bovine limbs. The described technique renders a complete filling of the vasculature of the claw even in regions that hitherto proved to be difficult regarding completeness of filling such...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990501)45:3<184::AID-JEM
更新日期:1999-05-01 00:00:00
abstract::The opossum Monodelphis domestica presents movement of the temporomandibular joint (TMJ) reflecting adaptation to eating habits similar to movement in humans, but the structure of the TMJ is not yet known. Thus, nine young M. domestica, of both sexes were weighed, anesthetized with xylazine (10 mg kg(-1) ), and ketami...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22702
更新日期:2016-09-01 00:00:00
abstract::The effects of short-term genistein exposure on ovarian folliculogenesis in immature rats were examined stereologically. To determine whether genistein acts as an estrogen agonist or antagonist, the results were compared with the effects of 17α-ethynylestradiol. Immature female rats received 50 mg/kg/bw of genistein i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22117
更新日期:2012-12-01 00:00:00
abstract::This study investigated the relationship between enteroendocrine and mucus-secreting cells distribution, the severity of colonic mucosal injury and intestinal motility in experimental colorectal carcinogenesis. Using a standardized murine model of colorectal carcinogenesis, eight-weeks-old female Wistar rats weighting...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22534
更新日期:2016-01-01 00:00:00
abstract::Numerous studies using morphologic techniques have demonstrated the plasticity of pulmonary epithelial cells and the requirement for complex intercellular interactions for regeneration of normal epithelium following lung injury. Recent developments in the molecular characterization of genes expressed in the lung have ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970901)38:5<473::AID-JEM
更新日期:1997-09-01 00:00:00
abstract::Orthodontic appliances causes specific alterations in oral environment, including reduction of pH, increase of dental biofilm and elevation of salivary microbial levels, causing an increased risk for dental caries. This study evaluated, using microbial culture and scanning electron microscopy (SEM), the in situ contam...
journal_title:Microscopy research and technique
pub_type: 杂志文章,随机对照试验
doi:10.1002/jemt.22817
更新日期:2017-05-01 00:00:00
abstract::Nowadays, infertility is no longer considered as an unsolvable disorder due to progresses in germ cells derived from stem lineage with diverse origins. Technical and ethical challenges push researchers to investigate various tissue sources to approach more efficient gametes. The purpose of the current study is to inve...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22880
更新日期:2017-11-01 00:00:00
abstract::STM tips made from antiferromagnetic MnNi have been used to investigate the atomic structure of the (001) and (111) surfaces of Fe3O4. The clean (001) surface displays a ( radical2 x radical2)R45 degrees superlattice, which is attributed to charge-ordering on the surface, where Fe(2+)-Fe2+ and Fe(3+)-Fe3+ dimers can b...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20148
更新日期:2005-02-01 00:00:00
abstract::An energy-filtering transmission electron microscope with 300 kV acceleration voltage was developed and the spatial resolution of elemental distribution images was improved. Observing oxygen monolayers in Al(11)O(3)N(9), it was shown that the actual resolution attained is up to 0.5 nm. Surface plasmon loss images of s...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20025
更新日期:2004-02-15 00:00:00
abstract::Regeneration of skeletal parts of fossil echinoderms is reviewed. Among regenerations of various fossil echinoderms, those of crinoids are most common and many examples have been reported. Mesozoic and Cenozoic crinoids, the subclass Articulata, are characterized by the dominance of muscular articulations in the arms ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1186
更新日期:2001-12-15 00:00:00
abstract::This study represents the first definitive anatomical description of the tongue and lingual papillae of the pampas deer and compares the different information on the morphology of the other ruminant species available in the literature. In this study, the tongues of four adult and one fetal deer were used. The tongue w...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22263
更新日期:2013-10-01 00:00:00
abstract::A synchrotron radiation fiber diffraction structural study of the axial filament of siliceous spicules from two species of marine sponges (the Demosponge Geodia cydonium and the Hexactinellid Scolymastra joubini) was carried out. The sharpness of the spots in the diffraction patterns indicated that the protein units i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10403
更新日期:2003-11-01 00:00:00
abstract::In present study, multiple microscope techniques were used for the systematics identification of the species Asplenium dalhousiae. The plant was collected from different phytogeographical and its natural habitat of Pakistan, where it shows higher diversity. Morphology, foliar epidermal anatomy, and spore morphological...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23189
更新日期:2019-04-01 00:00:00