Ultrastructural and functional connectivity of intracellularly stained neurones in the vertebrate retina: correlative analyses.

Abstract:

:A variety of intracellular recording and staining techniques has been used to establish structure-function and, in some cases, structure-function-neurochemical correlations in fish, turtle, and cat retinae. Cone photoreceptor-horizontal cell connectivity has been studied extensively in the cyprinid fish retina by intracellular staining with horseradish peroxidase (HRP) and subsequent electron microscopy. The available data suggest that horizontal cell dendrites around the ridge of the synaptic ribbon are postsynaptic, whilst finger-like extensions ("spinules") of lateral dendrites function as inhibitory feedback terminals. An interesting feature of this interaction is its plasticity: the feedback pathway is suppressed in the dark and becomes potentiated by light adaptation of the retina. Intracellular recordings and stainings of ganglion cells in both turtle and cat retinae have been possible. Prelabelling of ganglion cells by retrograde transport of rhodamine from the tectum allows ganglion cells to be stained under visual control, and their synaptic inputs determined by electron microscopy. Such studies have been extended to double labelling by using autoradiography or postembedding immunohistochemistry to identify the neurotransmitter content of the labelled cell and/or the neurotransmitter(s) converging upon it. It is envisaged that further applications of intracellular staining followed by double- or even triple-labelling will continue to enhance greatly our understanding of the functional architecture of the vertebrate retina.

journal_name

Microsc Res Tech

authors

Djamgoz MB,Kolb H

doi

10.1002/jemt.1070240106

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

43-66

issue

1

eissn

1059-910X

issn

1097-0029

journal_volume

24

pub_type

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