Genistein affects ovarian folliculogenesis: a stereological study.

Abstract:

:The effects of short-term genistein exposure on ovarian folliculogenesis in immature rats were examined stereologically. To determine whether genistein acts as an estrogen agonist or antagonist, the results were compared with the effects of 17α-ethynylestradiol. Immature female rats received 50 mg/kg/bw of genistein in dimethyl sulfoxide subcutaneously daily for three consecutive days from 18 to 20 days. The second group was injected with 1 μg/kg/bw of 17α-ethynylestradiol in olive oil in the same schedule. Each group had a corresponding control. Genistein increased ovary and ovarian stroma volumes by 18.50% (P < 0.05) and 53.40% (P < 0.05), respectively, and changed the parenchyma to stroma ratio in favor of stroma. Genistein induced decreases in the number of primordial (by 17.23%; P < 0.05), primary (16.62%; P < 0.05), and secondary follicles (12.29%: P < 0.05), whereas the number of atretic secondary follicles increased (5.10-fold; P < 0.05). The number of healthy large follicles was raised by 27.3% (P < 0.05), accompanied by 35.64% more atretic large follicles (P < 0.05). Similarly to genistein, estradiol changed the parenchyma to stroma ratio in favor of stroma, and reduced the number of primordial follicles, but the number of primary follicles was elevated. There were more healthy and atretic small and large follicles. In conclusion, genistein acted as an estrogen antagonist and had an inhibitory effect on the initial phase of folliculogenesis. In the other phases, genistein acted as an estrogen agonist, stimulating transition from the preantral to antral stage of folliculogenesis, and altering the ratio of follicular parenchyma and ovarian stroma in favor of stroma.

journal_name

Microsc Res Tech

authors

Medigović I,Ristić N,Trifunović S,Manojlović-Stojanoski M,Milošević V,Zikić D,Nestorović N

doi

10.1002/jemt.22117

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

1691-9

issue

12

eissn

1059-910X

issn

1097-0029

journal_volume

75

pub_type

杂志文章
  • Bones Morphogenic Protein-4 and retinoic acid combined treatment comparative analysis for in vitro differentiation potential of murine mesenchymal stem cells derived from bone marrow and adipose tissue into germ cells.

    abstract::Nowadays, infertility is no longer considered as an unsolvable disorder due to progresses in germ cells derived from stem lineage with diverse origins. Technical and ethical challenges push researchers to investigate various tissue sources to approach more efficient gametes. The purpose of the current study is to inve...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22880

    authors: Shirzeyli MH,Khanlarkhani N,Amidi F,Shirzeyli FH,Aval FS,Sobhani A

    更新日期:2017-11-01 00:00:00

  • Monolayer and three-dimensional cell culture and living tissue culture of gallbladder epithelium.

    abstract::Several models for preparing and isolating human and animal gallbladder epithelial cells, including low-grade gallbladder carcinoma cells, as well as proposed systems for culturing these isolated epithelial cells are reviewed here. Several reports concerning tissue culture of the gallbladder are also reviewed. The cel...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19971001)39:1<71::AID-JEMT

    authors: Nakanuma Y,Katayanagi K,Kawamura Y,Yoshida K

    更新日期:1997-10-01 00:00:00

  • Synaptic organization of amphibian sympathetic ganglia.

    abstract::The synaptic organization of the amphibian sympathetic ganglia was studied, especially in the last two abdominal paravertebral ganglia of the frog. These ganglia appear to form a monosynaptic relay, not containing interneurons. They consist of two systems working in parallel: the principal neurons, by far the most num...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19961001)35:2<157::AID-JEM

    authors: Lascar G,Eugene D,Taxi J

    更新日期:1996-10-01 00:00:00

  • Müllerian inhibiting substance: new perspectives and future directions.

    abstract::MIS, as a differentiate and antiproliferative agent, is precisely regulated, for example, at the transcriptional level by such transacting factors as SRY, and posttranslationally by testosterone. Processing of MIS most likely requires an as yet unknown in vivo protease which probably serves to control cleavage of MIS ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1070250205

    authors: Catlin EA,MacLaughlin DT,Donahoe PK

    更新日期:1993-06-01 00:00:00

  • Development of histamine-immunoreactivity in the Central nervous system of the two locust species Schistocerca gregaria and Locusta migratoria.

    abstract::Locusts are attractive model preparations for cellular investigations of neurodevelopment. In this study, we investigate the immunocytochemical localization of histamine in the developing ventral nerve cord of two locust species, Schistocerca gregaria and Locusta migratoria. Histamine is the fast neurotransmitter of p...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20980

    authors: Pätschke A,Bicker G

    更新日期:2011-10-01 00:00:00

  • Pollen morphological assessment of specific selected species of district Sheikhupura, Punjab, Pakistan under LM and SEM.

    abstract::Around 30 pollen samples, 16 families and 25 genera that were gathered, identified and preserved from 6 different localities of District Sheikhupura. Light and scanning electron microscopy was done for all collected flora. Out of the total samples, 43% specie was identified as herbs, 23% as shrubs, and 33% were identi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23656

    authors: Alam A,Shaheen S,Sarwar S,Jaffer M,Saba M,Hanif M,Sajjad S,Khan F

    更新日期:2020-12-31 00:00:00

  • Visualizing subsurface defects in graphite by acoustic atomic force microscopy.

    abstract::We describe a versatile platform, which combines atomic force acoustic microscopy, ultrasonic atomic force microscopy and heterodyne force microscopy. The AFM system can enable in-situ switching among these operation modes flexibly and thus benefit the discrimination of differences in mechanical properties and buried ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22668

    authors: Wang T,Ma C,Hu W,Chen Y,Chu J

    更新日期:2017-01-01 00:00:00

  • F463L increases the potential of dofetilide on human ether-a-go-go-related gene (hERG) channels.

    abstract::Mutations in genes related to long QT syndrome (LQTS) is recognized as an independent risk of drug-induced LQTS. We previously screened a mutation F463L in a Chinese patient with LQT2, syncope, and epilepsy. Here, we planned to illustrate how F463L influences the action of dofetilide on hERG channels. F463L-hERG plasm...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23021

    authors: Cheng G,Wu J,Han W,Sun C

    更新日期:2018-06-01 00:00:00

  • Germinal vesicle chromatin configurations of bovine oocytes.

    abstract::A common feature in the configuration of germinal vesicle (GV) chromatin in most species is that diffuse chromatin condenses into a perinucleolar ring during follicular growth; however, no such ring was observed in goat oocytes. Reports on whether bovine GV chromatin condenses into a perinucleolar ring are controversi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20349

    authors: Liu Y,Sui HS,Wang HL,Yuan JH,Luo MJ,Xia P,Tan JH

    更新日期:2006-10-01 00:00:00

  • Retinal characterization in the eyes of two bats endemic in the Egyptian fauna, the Egyptian fruit bat (Rousettus aegyptiacus) and insectivorous bat (Pipistrellus kuhlii), using the light microscope and transmission electron microscope.

    abstract::Bats are the only mammals that can fly in the dark without eye usage. This study was conducted to describe the structural and functional adaptations of the retina of two bats very common in the Egyptian fauna having a different lifestyle: the Egyptian fruit bat (Rousettus aegyptiacus) and insectivorous bat (Pipistrell...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23530

    authors: Aboelnour A,Noreldin AE,Massoud D Dr,Abumandour MMA

    更新日期:2020-11-01 00:00:00

  • MRT letter: segmentation and texture-based classification of breast mammogram images.

    abstract::Breast cancer is the most common cancer diagnosed among women. In this article, support vector machine is used to classify digital mammogram images into malignant and benign. Wiener filter is used to handle the possible quantum noise, which is more likely to occur in mammograms. Stack-based connected component method ...

    journal_title:Microscopy research and technique

    pub_type: 信件

    doi:10.1002/jemt.21070

    authors: Naveed N,Jaffar MA,Choi TS

    更新日期:2011-11-01 00:00:00

  • Diffracted-light contrast enhancement: a re-examination of oblique illumination.

    abstract::A re-examination and modification of the very old oblique illumination technique has resulted in a method for contrast enhancement in microscopes, diffracted-light contrast (DLC), which provides high-contrast, high-resolution images of unstained biological material. The technique, which utilizes the diffracted light f...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19990815/01)46:4/5<334::AI

    authors: Piekos WB

    更新日期:1999-08-15 00:00:00

  • Relationships between the central spindle and the contractile ring during cytokinesis in animal cells.

    abstract::During late anaphase and telophase, animal cells develop a bundle of antiparallel, interdigitating microtubules between the two daughter nuclei. Recent data indicate that this structure, called the central spindle, plays an essential role during cytokinesis. Studies in Drosophila and on vertebrate cells strongly sugge...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(20000415)49:2<202::AID-JEM

    authors: Gatti M,Giansanti MG,Bonaccorsi S

    更新日期:2000-04-15 00:00:00

  • Fluorescence intensity is a poor predictor of saturation effects in two-photon microscopy: artifacts in fluorescence correlation spectroscopy.

    abstract::Fluorescence correlation spectroscopy (FCS) has become an increasingly important measurement tool for biological and biomedical investigations, with the capability to assay molecular dynamics and interactions both in vitro and within living cells. Information recovery in FCS requires an accurate characterization and c...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20454

    authors: Wu J,Berland K

    更新日期:2007-08-01 00:00:00

  • From oogonia to mature oocytes: inactivation of the maternal centrosome in humans.

    abstract::The fine structure of human oogonia and growing oocytes has been reviewed in fetal and adult ovaries. Preovulatory maturation and the ultrastructure of stimulated oocytes from the germinal vesicle (GV) stage to metaphase II (MII) stage are also documented. Oogonia have large nuclei, scanty cytoplasm with complex mitoc...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.20299

    authors: Sathananthan AH,Selvaraj K,Girijashankar ML,Ganesh V,Selvaraj P,Trounson AO

    更新日期:2006-06-01 00:00:00

  • Effects of ultraviolet B radiation on physicochemical properties of human hair shaft.

    abstract::Changes in physicochemical properties of the human hair shaft induced by UVB during the summer climate were investigated. Using atomic force microscopy and thermogravimetric analyzer measurements, we investigated the changes in hair shaft physical properties including morphology, roughness, and water content. The chan...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22019

    authors: Kim KS,Shin MK,Park HK

    更新日期:2012-07-01 00:00:00

  • Effects of vitamin A deficiency on the inter-Sertoli cell tight junctions and on the germ cell population.

    abstract::When 20-day-old rats are placed on a vitamin A deficient diet (VAD) for a period of 10 weeks, the seminiferous tubules are found to contain only Sertoli cells, a few residual A0, A1 spermatogonia, and preleptotene spermatocytes (PL). The type A1 spermatogonia and PL spermatocytes are arrested in their G2 phase. In VAD...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070200106

    authors: Ismail N,Morales CR

    更新日期:1992-01-01 00:00:00

  • Intelligent microscopic approach for identification and recognition of citrus deformities.

    abstract::Plant diseases are accountable for economic losses in an agricultural country. The manual process of plant diseases diagnosis is a key challenge from last one decade; therefore, researchers in this area introduced automated systems. In this research work, automated system is proposed for citrus fruit diseases recognit...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23320

    authors: Safdar A,Khan MA,Shah JH,Sharif M,Saba T,Rehman A,Javed K,Khan JA

    更新日期:2019-09-01 00:00:00

  • Postembryonic development of hard jaws (trophi) in a species belonging to the Brachionus plicatilis complex (Rotifera, Monogononta): a morphometric analysis.

    abstract::The presence of hard jaws (trophi), with species-specific shape and size, is a taxonomic feature of Rotifera, a group of microscopic metazoans. Since trophi are used to discriminate among species, it is important to know whether these structures change in taxonomically important ways during postembryonic development. ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20319

    authors: Fontaneto D,Melone G

    更新日期:2006-04-01 00:00:00

  • Microscopic and molecular evidence in support of rodent as a reservoir for dissemination of Leishmaniasis.

    abstract::Leishmaniasis is a worldwide public health problem and vector-borne disease. It is caused by a diverse group of protozoan parasites that belong to the genus Leishmania and transmitted to humans through a bite of an infected female sand fly. Leishmaniasis has attained epidemic proportion in Khyber Pakhtunkhwa and raise...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23462

    authors: Khan I,Noor Z,Allauddin Khan S,Ali Khan T,Muhammad N,Ullah Khattak B,Hussain M

    更新日期:2020-07-01 00:00:00

  • Neurotrophin-mediated potentiation of neuronal injury.

    abstract::The neurotrophins are a diverse family of peptides which activate specific tyrosine kinase-linked receptors. Over the past five decades, since the pioneering work of Levi-Montalcini and colleagues, the critical role that neurotrophins play in shaping the developing nervous system has become increasingly established. T...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19990515/01)45:4/5<276::AI

    authors: Behrens MM,Strasser U,Lobner D,Dugan LL

    更新日期:1999-05-15 00:00:00

  • Nitric oxide modulates sympathetic neurotransmission at the prejunctional level.

    abstract::In spite of accumulating evidence for a modulation of sympathetic neurotransmission by endogenously produced nitric oxide (NO), it remains unclear in which parts of the vascular system and at what level this interaction takes place. The aim of the present study was to investigate the distribution of endothelial and ne...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070290214

    authors: Addicks K,Bloch W,Feelisch M

    更新日期:1994-10-01 00:00:00

  • Osteoclastic bone resorption around intraosseous screws in rat and pig mandibles.

    abstract::This report describes osteoclastic bone resorption around intraosseous fixation screws in rat and pig mandibles. These screws supported distraction devices and provided for neutral fixation following the distraction period. Progressive clinical instability of bicortical screws and radiographic and histologic evidence ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.10376

    authors: Schulten AJ,Zimmermann CE,Glowacki J

    更新日期:2003-08-15 00:00:00

  • The comparison between force volume and peakforce quantitative nanomechanical mode of atomic force microscope in detecting cell's mechanical properties.

    abstract::Atomic force microscope (AFM) has been widely used in the biological field owing to its high sensitivity (subnanonewton), high spatial resolution (nanometer), and adaptability to physiological environments. Nowadays, force volume (FV) and peakforce quantitative nanomechanical (QNM) are two distinct modes of AFM used i...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23351

    authors: Yang Y,Xiao X,Peng Y,Yang C,Wu S,Liu Y,Yue T,Pu H,Liu N,Jiang H

    更新日期:2019-11-01 00:00:00

  • Red pigments and Boraginaceae leaves in mortuary ritual of late Neolithic China: A case study of Shengedaliang site.

    abstract::Investigation of mortuary ritual is an important method to reconstruct many aspects of past societies. Due to the lack of relevant analytical work, little evidence related to organic materials in a burial can be found in China. Here we report materials collected from a burial during the excavation of the Shengedaliang...

    journal_title:Microscopy research and technique

    pub_type: 历史文章,杂志文章

    doi:10.1002/jemt.22791

    authors: Wu Y,Guo X,Wang W,Chen X,Zhao Z,Xia X,Yang Y

    更新日期:2017-02-01 00:00:00

  • Comparison of SEM processing methods for cultured human lens epithelial cells grown on flat and microcarrier bead substrates.

    abstract::The increasing importance of in vitro models has presented new challenges in SEM processing techniques. The present study has evaluated the quality of preservation of cultured human lens epithelial cells processed by critical point, Peldri II, and tert-butyl alcohol drying. Specimens processed by critical point drying...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070300508

    authors: Cantu-Crouch D,Howe WE,McCartney MD

    更新日期:1995-04-01 00:00:00

  • Effect of age on the structure of Meissner corpuscles in murine digital pads.

    abstract::A light and electron microscopic study was performed to determine age changes in Meissner corpuscles. In forepaw digital pads of mice aged to their maximum life expectancy, corpuscles were found to increase in size and complexity until middle age, and then to become smaller, disorganized and lobulated with more advanc...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19960701)34:4<376::AID-JEM

    authors: Nava PB,Mathewson RC

    更新日期:1996-07-01 00:00:00

  • Involvement of actin microfilaments in the transcription/replication of human parainfluenza virus type 3: possible role of actin in other viruses.

    abstract::Multifunctional involvement of actin microfilaments during viral infection has been documented in many studies. The molecular mechanism underlying this important host-virus interaction, however, remains poorly understood. We have investigated the role of actin microfilaments in the life cycle of human parainfluenza vi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19991015)47:2<114::AID-JEM

    authors: De BP,Banerjee AK

    更新日期:1999-10-15 00:00:00

  • Cartilage-derived morphogenetic proteins and cartilage morphogenesis.

    abstract::Cartilage morphogenesis is a prerequisite for skeletal development and maintenance. The morphogenesis of cartilage determines the shape of bones, and joints including articular cartilage, ligaments, and tendon. This article reviews the recent advances in cartilage-derived morphogenetic proteins (CDMPs) and related bon...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19981015)43:2<131::AID-JEM

    authors: Reddi AH

    更新日期:1998-10-15 00:00:00

  • An effective and accurate identification system of Mycobacterium tuberculosis using convolution neural networks.

    abstract::Tuberculosis (TB) remains the leading cause of morbidity and mortality from infectious disease in developing countries. The sputum smear microscopy remains the primary diagnostic laboratory test. However, microscopic examination is always time-consuming and tedious. Therefore, an effective computer-aided image identif...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23217

    authors: Kuok CP,Horng MH,Liao YM,Chow NH,Sun YN

    更新日期:2019-06-01 00:00:00