Nitric oxide modulates sympathetic neurotransmission at the prejunctional level.

Abstract:

:In spite of accumulating evidence for a modulation of sympathetic neurotransmission by endogenously produced nitric oxide (NO), it remains unclear in which parts of the vascular system and at what level this interaction takes place. The aim of the present study was to investigate the distribution of endothelial and neuronal NO synthase (NOS) along the vascular tree of the heart at the light and electron microscopic level using NADPH-diaphorase (NADPH-d) staining as a marker for NOS. In addition, the functional effects of exogenous NO on coronary vascular resistance and cardiac adrenergic nerves was studied using the isolated perfused rat heart as a model. The intraaxonal catecholamine content of adrenergic nerve fibers was visualised and morphometrically assessed by applying glyoxylic acid-induced histofluorescence. The expression of endothelial NOS in the heart was found to depend on the diameter of the blood vessel. Arteries > 100 microns always showed intense staining, whereas staining in smaller arteries and veins was considerably weaker. Smooth-muscle free vessels were essentially devoid of NADPH-d activity. In atrial and ventricular myocardium, neuronal NOS localised in autonomic nerve fibers along the entire vascular tree. Ultrastructurally, NADPH-d staining revealed adjacent localisation of NOS-positive and -negative axons, suggesting and interaxonal modulation of adjacent autonomic nerve fibers by NO. In isolated perfused rat hearts, the intracoronary application of 10(-8) M NO produced a marked decrease of coronary perfusion pressure, which was accompanied by a distinct increase in intraaxonal catecholamine levels of intramural adrenergic nerve fibers.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Microsc Res Tech

authors

Addicks K,Bloch W,Feelisch M

doi

10.1002/jemt.1070290214

subject

Has Abstract

pub_date

1994-10-01 00:00:00

pages

161-8

issue

2

eissn

1059-910X

issn

1097-0029

journal_volume

29

pub_type

杂志文章
  • Spatial distribution of cytoskeleton intermediate filaments during fetal rat hepatocyte differentiation.

    abstract::The construction of the liver parenchyma throughout fetal development depends on the elaboration of intercellular contacts between epithelial cells and between epithelial and mesenchymal cells. During this time, the spatial distribution of cytokeratins in hepatocytes shows a striking evolution as demonstrated by confo...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19971201)39:5<436::AID-JEM

    authors: Vassy J,Irinopoulou T,Beil M,Rigaut JP

    更新日期:1997-12-01 00:00:00

  • Citron, a Rho target that affects contractility during cytokinesis.

    abstract::The small GTPase Rho, which regulates cell shape, is thought to contribute to cytokinesis. Recently, Citron was characterized as a Rho target. This large protein contains a Ser/Thr kinase domain related to that of ROCK, another Rho effector. Both endogenous Citron and recombinant Citron localize to the cleavage furrow...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(20000415)49:2<123::AID-JEM

    authors: Madaule P,Furuyashiki T,Eda M,Bito H,Ishizaki T,Narumiya S

    更新日期:2000-04-15 00:00:00

  • Methods for characterizing plant fibers.

    abstract::The effectiveness of different microscopy techniques for measuring the dimensions of ultimate fibers from harakeke (Phormium tenax, New Zealand flax) was investigated using a factorial experimental design. Constant variables were geographical location, location of specimens along the leaf, season (winter), individual ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20206

    authors: Cruthers N,Carr D,Niven B,Girvan E,Laing R

    更新日期:2005-08-01 00:00:00

  • Impact of screening on prostate cancer rates and trends.

    abstract::Widespread implementation of prostate cancer screening has affected several epidemiologic features of the disease including incidence, tumor and patient characteristics, patterns of care and outcomes. Some of these changes have been interpreted as evidence of the success of PSA testing as a cancer control strategy. Da...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/1097-0029(20001201)51:5<415::AID-JEMT2>3.0

    authors: Mettlin C

    更新日期:2000-12-01 00:00:00

  • The effect of different thermal treatment on the physical properties of PGA/PCL copolymer suture material.

    abstract::Samples of polyglycolic acid PGA/polycaprolactone PCL copolymer absorbable suture materials, which is called Poliglecaprone PGC copolymer suture, were thermally treated by two different processes. The first method is the direct heat exposure, from 20 to 38 °C, in the surrounding medium. Which is an environmental study...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22957

    authors: El-Farahaty KA,El-Bakary MA,El-Sayed NM

    更新日期:2018-01-01 00:00:00

  • Analysis of the interfacial micromorphology of adhesive systems in cavities prepared with Er,Cr:YSGG, Er:YAG laser and bur.

    abstract::The purpose of this in vitro study was to evaluate the interaction pattern of adhesive systems on laser and bur cavities. Cavities were prepared according to the following groups (n=9): (G1) conventional diamond bur (No. 1013); (G2) Er:YAG laser (250 mJ, 4 Hz, 80.6 J/cm2); (G3) Er,Cr:YSGG laser (3.5 W, 20 Hz, 61.7 J/c...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20459

    authors: Aranha AC,De Paula Eduardo C,Gutknecht N,Marques MM,Ramalho KM,Apel C

    更新日期:2007-08-01 00:00:00

  • Sorting and reorganization of centrosomes during oocyte maturation in the mouse.

    abstract::In animal oocytes, the centrosome exists as an acentriolar aggregate of centrosomal material that is regulated in a dynamic manner throughout the process of meiotic maturation. Recently, it has been demonstrated that in female meiotic systems spindle assembly is likely regulated by chromosomal and microtubule/microtub...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(20000601)49:5<435::AID-JEM

    authors: Carabatsos MJ,Combelles CM,Messinger SM,Albertini DF

    更新日期:2000-06-01 00:00:00

  • Transformation of external sensilla to chordotonal sensilla in the cut mutant of Drosophila assessed by single-cell marking in the embryo and larva.

    abstract::The cut gene of Drosophila melanogaster is an identity selector gene that establishes the program of development and differentiation of external sense organs. Mutations in the cut gene cause a transformation of the external sense organs into chordotonal organs, originally assessed by the use of immunostaining methods ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19971215)39:6<492::AID-JEM

    authors: Merritt DJ

    更新日期:1997-12-15 00:00:00

  • Characterization of the alimentary canal and Malpighian tubules of Chrysolina herbacea (Duftschmid, 1825) (Coleoptera: Chrysomelidae): Anatomical and histological approaches.

    abstract::The mint beetle or green mint beetle, Chrysolina herbacea, is one of the most important insect pests of mint herb. These insects are associated with plants belonging to the Lamiaceae family, in particular the Mentha genus. They cause serious damages to plants by consuming their leaves. For this reason, it is an import...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23671

    authors: Özyurt Koçakoğlu N,Candan S

    更新日期:2020-12-11 00:00:00

  • A polyaniline based intrinsically conducting coating for corrosion protection of structural steels.

    abstract::Among the various corrosion protection strategies for structural steels, coating techniques provide the most cost-effective protection and have been used as the primary mode of corrosion protection. Existing coating techniques however have been used mainly for their barrier capability and therefore all have a limited ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22283

    authors: Pan T,Wang Z

    更新日期:2013-11-01 00:00:00

  • One-step preparation of hybrid materials of polyacrylamide networks and gold nanoparticles.

    abstract::Hybrid materials of polyacrylamide networks and gold nanoparticles were prepared by directly heating an aqueous solution containing HAuCl(4), acrylamide, N,N'-methylenebis-acrylamide, and sodium sulfite (Na(2)SO(3)). Acrylamide, N,N'-methylenebis-acrylamide, and Na(2)SO(3) were used as monomers, crosslinking agent, an...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20568

    authors: Song Y,Li Z,Wang L,Yao Y,Chen C,Cui K

    更新日期:2008-06-01 00:00:00

  • User-friendly semiautomated assembly of accurate image mosaics in microscopy.

    abstract::We present a semiautomated software solution to the problem of extending the lateral field of view of a classical microscope. The initial requirements are a set of overlapping images, along with their user-provided coarse mosaic. Our solution then refines this initial mosaic in a fully automatic fashion. We rely on a ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20393

    authors: Thévenaz P,Unser M

    更新日期:2007-02-01 00:00:00

  • Biochemical and morphological alterations in the Achilles tendon of mdx mice.

    abstract::Dystrophin-deficient muscles have repeated cycles of necrosis and regeneration, being susceptible to injury induced by muscle contractions. Some studies have demonstrated that tendons are also affected in mdx mice, based especially on the changes in biomechanical properties arising from the respective linked muscles. ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22448

    authors: De Aro AA,Guerra Fda R,Esquisatto MA,Nakagaki WR,Gomes L,Pimentel ER

    更新日期:2015-01-01 00:00:00

  • Round window membrane. Structure function and permeability: a review.

    abstract::The ultrastructure of the round window membrane of humans, monkeys, felines, and rodents discloses three basic layers: an outer epithelium, a middle core of connective tissue, and an inner epithelium. Interspecies variations are mainly in terms of thickness, being thinnest in rodents and thicker in humans. Morphologic...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19970201)36:3<201::AID-JEM

    authors: Goycoolea MV,Lundman L

    更新日期:1997-02-01 00:00:00

  • Genistein affects ovarian folliculogenesis: a stereological study.

    abstract::The effects of short-term genistein exposure on ovarian folliculogenesis in immature rats were examined stereologically. To determine whether genistein acts as an estrogen agonist or antagonist, the results were compared with the effects of 17α-ethynylestradiol. Immature female rats received 50 mg/kg/bw of genistein i...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22117

    authors: Medigović I,Ristić N,Trifunović S,Manojlović-Stojanoski M,Milošević V,Zikić D,Nestorović N

    更新日期:2012-12-01 00:00:00

  • Three-dimensional structure of the rat pancreatic duct in normal and inflammated pancreas.

    abstract::We observed the corrosion casts of the Wistar rats' pancreatic ducts with scanning electron microscopy (SEM), and their conventionally fixed pancreatic tissue with SEM and transmission electron microscopy (TEM). These findings revealed the following facts about the three-dimensional structure of pancreatic duct. (1) T...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19970601)37:5/6<543::AID-J

    authors: Ashizawa N,Endoh H,Hidaka K,Watanabe M,Fukumoto S

    更新日期:1997-06-01 00:00:00

  • Uranium-contaminated soils: ultramicrotomy and electron beam analysis.

    abstract::Uranium-contaminated soils from the U.S. Department of Energy (DOE) Fernald Site, Ohio, have been examined by a combination of backscattered electron imaging (BSE) and analytical electron microscopy with electron diffraction (AEM). The inhomogeneous distribution of particulate uranium phases in the soil required the d...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070310208

    authors: Buck EC,Dietz NL,Bates JK

    更新日期:1995-06-01 00:00:00

  • Imaging of three-dimensional epithelial architecture and function in cultured CaCo2a monolayers with two-photon excitation microscopy.

    abstract::The principal functions of the gastrointestinal tract mucosa include nutrient absorption, protein and fluid secretion, and the regulated symbiosis with intraluminal contents. Research in epithelial biology has benefited significantly from the use of cultured monolayer preparations, which closely replicate the structur...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/1097-0029(20001015)51:2<204::AID-JEMT11>3.

    authors: Gilbert RJ,Hoffman M,Capitano A,So PT

    更新日期:2000-10-15 00:00:00

  • Analysis of dynamic cantilever behavior in tapping mode atomic force microscopy.

    abstract::Tapping mode atomic force microscopy (AFM) provides phase images in addition to height and amplitude images. Although the behavior of tapping mode AFM has been investigated using mathematical modeling, comprehensive understanding of the behavior of tapping mode AFM still poses a significant challenge to the AFM commun...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22558

    authors: Deng W,Zhang GM,Murphy MF,Lilley F,Harvey DM,Burton DR

    更新日期:2015-10-01 00:00:00

  • Facilitating the pickup of individual DNA molecules by AFM nanomanipulation with tips mechanically worn on bare mica.

    abstract::The tip is one of the critical factors to improve the efficiency in picking up individual DNA molecules from solid substrates based on atomic force microscope (AFM) nanomanipulation. We found that wearing AFM tips on certain solid substrates in advance to nanomanipulation operation would largely improve the pickup eff...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.21104

    authors: Duan N,Long F,Wang X,Li B,Hu J,Zhang Y

    更新日期:2012-05-01 00:00:00

  • Ultrastructural study on the body surface of the acanthocephalan parasite Dentitruncus truttae in brown trout.

    abstract::Scanning electron microscope (SEM) investigations on the holdfast elements, proboscis hooks, and trunk spines of Dentitruncus truttae (Acanthocephala, Palaeacanthocephala), an endoparasite of Salmo trutta (brown trout), provide more data about the surface of these taxonomic relevant structures. In both acanthocephalan...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20543

    authors: Dezfuli BS,Lui A,Giari L,Boldrini P,Giovinazzo G

    更新日期:2008-03-01 00:00:00

  • Fragile X syndrome, the Fragile X related proteins, and animal models.

    abstract::The Fragile X syndrome (FraX), which is characterized among other physical and neurologic impairments by mental retardation, is caused by the absence of the product of the FMR1 gene. The Fragile X Mental Retardation Protein (FMRP) is a member of a novel family of RNA-binding proteins. The latter includes two other pro...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.10064

    authors: Hoogeveen AT,Willemsen R,Oostra BA

    更新日期:2002-05-01 00:00:00

  • Ionic composition of rat airway surface liquid determined by X-ray microanalysis.

    abstract::The thin layer of liquid that lines the conducting airway epithelium, the airway surface liquid (ASL), is important for mucociliary clearance. Altered ionic composition and/ or volume of the ASL play a major role in the pathology of airway diseases such as cystic fibrosis. Since the ASL is a thin layer, it has been di...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20217

    authors: Vanthanouvong V,Kozlova I,Roomans GM

    更新日期:2005-09-01 00:00:00

  • Moving legs: A workflow on how to generate a flexible endopod of the 518 million-year-old Chengjiang arthropod Ercaicunia multinodosa using 3D-kinematics (Cambrian, China).

    abstract::Understanding the functional morphology and mobility of appendages of fossil animals is important for exploring ecological traits such as feeding and locomotion. Previous work on fossils from the 518 million-year-old Chengjiang biota of China was based mainly on two-dimensional information captured from the surface of...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23628

    authors: Schmidt M,Liu Y,Zhai D,Hou X,Melzer RR

    更新日期:2020-11-06 00:00:00

  • Comparison of alcian blue and ruthenium red effects on preservation of outer envelope ultrastructure in methanotrophic bacteria.

    abstract::Alcian blue (AB) and ruthenium red (RR) effects on ultrastructural preservation of the bacterial cell envelope of methanotrophs are compared. A previous successful method with RR that enhanced preservation of outer envelope layers in two representative methanotroph species is applied to other genera and species of met...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070200109

    authors: Fassel TA,Schaller MJ,Remsen CC

    更新日期:1992-01-01 00:00:00

  • Transgenic models of pituitary diseases.

    abstract::Transgenic mice are valuable experimental models of human endocrine diseases. Targeted ablation of specific cell lineages or insertion of genes coding for releasing factors, hormones, growth factors, and oncogenes fused with appropriate promoters, or mutated genes, can induce several pituitary disorders. Various hypos...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19971015)39:2<194::AID-JEM

    authors: Stefaneanu L,Kovacs K

    更新日期:1997-10-15 00:00:00

  • E-cadherin and catenins: molecules with versatile roles in normal and neoplastic epithelial cell biology.

    abstract::E-cadherin and its associated cytoplasmic proteins alpha-, beta-, and gamma-catenin, play a crucial role in epithelial cell-cell adhesion and in the maintenance of tissue architecture. Perturbation in the expression or function of any of these molecules results in loss of intercellular adhesion, with possible conseque...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19981101)43:3<224::AID-JEM

    authors: El-Bahrawy MA,Pignatelli M

    更新日期:1998-11-01 00:00:00

  • Cell-cell interactions in the testis of teleosts and elasmobranchs.

    abstract::In this paper we present the state of knowledge on cell-cell interactions in the testis of two groups of anamniote vertebrates--teleosts and elasmobranchs--which include most fish. In these fish, the structural organization of the testis differs fundamentally from that which characterizes amniotes in which the germina...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1070320606

    authors: Loir M,Sourdaine P,Mendis-Handagama SM,Jégou B

    更新日期:1995-12-15 00:00:00

  • Intrinsic physicochemical interactions of calcium hydroxide-based medications.

    abstract::To investigate intrinsic physicochemical properties and interactions of three different calcium hydroxide-based medications via means of different analytical methods. Two-commercial premixed medications: TempCanal(TCmx) and ProCalR(PCmx) and powder-form ProCal(PCpw) with glycerin were used. Vibrational modes were anal...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23600

    authors: Uslu O,Dikmen G,Orhan EO

    更新日期:2020-09-21 00:00:00

  • Cardiac neurones of autonomic ganglia.

    abstract::The properties of the postganglionic sympathetic neurones supplying the heart and arising in the stellate and adjacent paravertebral ganglia of various species are discussed with respect to their location, morphology, synaptic input and membrane characteristics. Results from our laboratory on the morphology of rat ste...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19960901)35:1<69::AID-JEMT

    authors: Wallis D,Watson AH,Mo N

    更新日期:1996-09-01 00:00:00