Abstract:
:We present a semiautomated software solution to the problem of extending the lateral field of view of a classical microscope. The initial requirements are a set of overlapping images, along with their user-provided coarse mosaic. Our solution then refines this initial mosaic in a fully automatic fashion. We rely on a highly accurate registration engine to perform the pairwise registration of the individual images, and on an efficient strategy to minimize the amount of computations while maintaining the highest possible global quality. We describe these ingredients, which we make available as a free multiplatform user-friendly software package. We also highlight why and how the specific aspects of the present microscopy application differ from those encountered while creating more common mosaics such as panoramas. Finally, we present experimental results that illustrate and validate our method on a real biological sample. We conclude by showing that we are able to reach subpixel accuracy.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Thévenaz P,Unser Mdoi
10.1002/jemt.20393subject
Has Abstractpub_date
2007-02-01 00:00:00pages
135-46issue
2eissn
1059-910Xissn
1097-0029journal_volume
70pub_type
杂志文章abstract::Acrylic resin mixtures are commonly used to study microscopic sections of biological specimens, giving the advantage of good morphological preservation. Existing embedding protocols, however, are suitable for tissue blocks, not exceeding 1 mm in thickness. We have developed a protocol to embed larger specimens (up to ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20139
更新日期:2005-01-01 00:00:00
abstract::The glycoconjugate pattern of developing ovarian follicles in wild and cultured Senegalese sole Solea senegalensis was investigated by means of lectin histochemistry. Ovaries from cultured fish contained oocytes up to the late vitellogenic stage, whereas they reached the hydration stage in wild specimens. The follicul...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22040
更新日期:2012-08-01 00:00:00
abstract::Gymnosporia arenicola Jordaan (Celastraceae) is a shrub or small tree, which naturally occurs in coastal sand dunes of Southern Mozambique and South Africa. Its dried leaf is often used in traditional medicine for the treatment of infectious and inflammatory diseases. Hereby, we present results of studies carried out ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22565
更新日期:2015-11-01 00:00:00
abstract::All organisms exhibit significant daily rhythms in a myriad of functions from molecular levels to the level of the whole organism. Significantly, most of these rhythms will persist under constant conditions, showing that they are driven by an internal circadian clock. In birds the circadian system is composed of sever...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1068
更新日期:2001-04-01 00:00:00
abstract::Mechanically dissociated neuronal cell bodies from the thoracic ganglia of Locusta migratoria were viable in culture conditions for up to 2 days and were voltage-clamped to record the effects of GABAergic drugs and physostigmine on the membrane conductance and ACh responses of the dissociated cells. Bicuculline, hydra...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10028
更新日期:2002-02-15 00:00:00
abstract::Dystrophin-deficient muscles have repeated cycles of necrosis and regeneration, being susceptible to injury induced by muscle contractions. Some studies have demonstrated that tendons are also affected in mdx mice, based especially on the changes in biomechanical properties arising from the respective linked muscles. ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22448
更新日期:2015-01-01 00:00:00
abstract::This study was conducted to provide comprehensive information on the anatomical and histological features of the upper (UL) and lower (LL) lips of Rahmani sheep by gross examination, morphometric analysis in addition to Light and scanning electron microscope (SEM). Samples from normal healthy adult male sheep heads we...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23660
更新日期:2020-12-01 00:00:00
abstract::The density of nodes of Ranvier was examined at CNS, PNS, and transitional zone (TZ) levels of rat lumbar ventral motoneurone fibres. It was found to be significantly greater in the TZ than at the other levels: The difference was sevenfold for the ventral root and at least fourfold for central fibre levels. Node distr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19960815)34:6<507::AID-JEM
更新日期:1996-08-15 00:00:00
abstract::We present a new beam-shaping technique for two-color excitation fluorescence microscopy. We show that by simply inserting a properly designed shaded-ring filter in the illumination beam of smaller wavelength, it is possible to improve the effective optical sectioning capacity of such microscopes by 23%. Such an impro...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20073
更新日期:2004-06-01 00:00:00
abstract::The tip is one of the critical factors to improve the efficiency in picking up individual DNA molecules from solid substrates based on atomic force microscope (AFM) nanomanipulation. We found that wearing AFM tips on certain solid substrates in advance to nanomanipulation operation would largely improve the pickup eff...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.21104
更新日期:2012-05-01 00:00:00
abstract::In order to test the effectiveness of a new advanced time-lapse microscopy imaging and image processing and analysis system, and to do quantitative and qualitative temporal analyses of in vitro peripheral nerve regeneration, long-term time-lapse imaging of cultures of mouse dorsal root ganglia (DRGs) was performed. DR...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20075
更新日期:2004-06-15 00:00:00
abstract::Adrenomedullin (AM) is a novel neuropeptide with special significance in the mammalian hypothalamo-hypophysial axis. By using an antiserum specific for human AM, we have studied the localization of AM-like immunoreactive (AMi) cell bodies and fibers in the hypothalamus and hypophysis of the amphibians Rana perezi (anu...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1131
更新日期:2001-08-01 00:00:00
abstract::Ribbon synapses differ from conventional chemical synapses in that they contain, within the cloud of synaptic vesicles (SV's), a specialized synaptic body, most often termed synaptic ribbon (SR). This body assumes various forms. Reconstructions reveal that what appear as rod- or ribbon-like profiles in sections are in...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19961215)35:6<472::AID-JEM
更新日期:1996-12-15 00:00:00
abstract::Alcian blue (AB) and ruthenium red (RR) effects on ultrastructural preservation of the bacterial cell envelope of methanotrophs are compared. A previous successful method with RR that enhanced preservation of outer envelope layers in two representative methanotroph species is applied to other genera and species of met...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070200109
更新日期:1992-01-01 00:00:00
abstract::Previous studies reported that intrageneric relationships of genus Veratrum of family Melanthiaceae are controversial and hard to delimit. Therefore, we observed the pollen morphological features of six species in the genus Veratrum in detail using both light microscopy and scanning electron microscopy and investigate...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23479
更新日期:2020-08-01 00:00:00
abstract::The catecholaminergic cell groups in the human brain, denominated from A1 to A17, display some striking anatomical differences with those described in the rodent. These differences are essentially observed in the extent of the dopaminergic neurons and especially their axonal fields in the telencephalon. Immunocytochem...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990701)46:1<24::AID-JEMT
更新日期:1999-07-01 00:00:00
abstract::Transfer of a fluorescently labeled protein particle from a surface to a microsized scanning probe has been induced by repetitive scanning in aqueous medium. The so-attached particle can in turn act as a probing tool to study particle-substrate and particle-particle interactions. Attachment of the fluorescent particle...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20816
更新日期:2010-08-01 00:00:00
abstract::Because of the morphological and macroscopic similarity, many species of Erigeron and Aster (Asteraceae) are confusable and usually used under the same name "Meiduoluomi" in traditional Tibetan medicine (TTM). To find an easy, quick, and reliable method to authenticate and distinguish the eight main medicinal plants o...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20725
更新日期:2009-10-01 00:00:00
abstract::Spermatogenesis is a process of division and differentiation by which spermatozoa are produced in seminiferous tubules. A measure of efficiency of spermatogenesis is the estimated number of spermatozoa produced per day per gram of testicular parenchyma. This measure is not influenced by species differences in testicul...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070320504
更新日期:1995-12-01 00:00:00
abstract::We discuss the use of fluorescence correlation spectroscopy for the measurement of relatively slow rotations of large macromolecules in solution or attached to other macromolecular structures. We present simulations and experimental results to illustrate the range of rotational correlation times and diffusion times th...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20121
更新日期:2004-11-01 00:00:00
abstract::In animal oocytes, the centrosome exists as an acentriolar aggregate of centrosomal material that is regulated in a dynamic manner throughout the process of meiotic maturation. Recently, it has been demonstrated that in female meiotic systems spindle assembly is likely regulated by chromosomal and microtubule/microtub...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(20000601)49:5<435::AID-JEM
更新日期:2000-06-01 00:00:00
abstract::Polycrystalline silver catalysts are used extensively for the partial oxidation of methanol to formaldehyde, which is then primarily incorporated in the synthesis process for adhesives and resins. In order to maximize formaldehyde production it is essential to gain a comprehensive understanding of the complex microstr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19970301)36:5<382::AID-JEM
更新日期:1997-03-01 00:00:00
abstract::From the first cases of bovine spongiform encephalopathy (BSE) among cattle in the United Kingdom in 1986, the route of infection of BSE is generally believed by means of feeds containing low level of processed animal proteins (PAPs). Therefore, many feed bans and alternative and complementary techniques were resulted...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20951
更新日期:2011-08-01 00:00:00
abstract::We have used high voltage electron microscopy and computer-generated three-dimensional reconstructions from serial sections to elucidate the structure of taste bud cells and their associated synapses in fungiform taste buds of the mouse. Five fungiform taste buds (two of which were serially sectioned) were examined wi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070260207
更新日期:1993-10-01 00:00:00
abstract:OBJECTIVES:This study investigated the effects of sliding on the ultrastructure of three representative esthetic superelastic 0.014 inch nickel-titanium (NiTi) archwires. METHODS:Atomic force microscopy, scanning electron microscopy, and light microscopy were used to estimate the surface roughness of archwires and bra...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22557
更新日期:2015-10-01 00:00:00
abstract::The dermal denticles are among the unique morphological adaptations of sharks, which have been acquired throughout their long evolutionary process of more than 400 million years. Species-specific morphological characteristics of these structures has been applied specially as tools for functional and taxonomic (family-...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23273
更新日期:2019-08-01 00:00:00
abstract::The purpose of this study is to determine the possible changes in the localization of the four Epidermal Growth Factor Receptors and three ligands in quail lungs from the first day of hatching until the 125th after hatching using immunohistochemical methods. Immunohistochemical results demonstrated that four EGFRs and...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22544
更新日期:2015-09-01 00:00:00
abstract::Scanning electron microscope images of complete and damaged frustules of Nitzschia marina Grun. revealed an unusual chambered structure of the valve. This structure is not present in any existing nitzschioid genus and warrants an independent generic status for this taxon, which is therefore separated into Alveus gen. ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(199601)33:1<2::AID-JEMT2>3
更新日期:1996-01-01 00:00:00
abstract::The Dominican Republic (DR) has experienced tremendous increase in the number of cases of dengue virus (DENV) reported in the past few years. There are four serotypes of DENV (1-4), and each can cause classic dengue fever (DF), dengue hemorrhagic fever (DHF), or dengue shock syndrome (DSS). DENV-1-4 are currently circ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20225
更新日期:2005-11-01 00:00:00
abstract::Micromass cultures represent a convenient means of studying chondrocyte physiology in the context of a tridimensional culture model. In this study, we present the first ultrastructural analysis of the distribution and organization of the extracellular components in micromasses in comparison with their cartilaginous co...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20210
更新日期:2005-08-15 00:00:00