Cell-cell interactions in the testis of teleosts and elasmobranchs.


:In this paper we present the state of knowledge on cell-cell interactions in the testis of two groups of anamniote vertebrates--teleosts and elasmobranchs--which include most fish. In these fish, the structural organization of the testis differs fundamentally from that which characterizes amniotes in which the germinal tissue is located in tubules open at both ends and consists of a permanent population of Sertoli cells associated with successive stages of germ cell development. In fish, the spermatogenic unit of testis is the spermatocyst, which corresponds to one germ cell or to a clone of isogenetic germ cells, enclosed by one or several Sertoli cells, which form the wall of the cyst. In fish testis, the Sertoli cells do not represent a permanent population of cells. Although both are of the cystic type, the teleost and elasmobranch testes are differently organized. In elasmobranchs, primary spermatogonia and Sertoli cells lie initially free within the interstitial tissue, before becoming sequestered by a basement membrane; the testis is then composed of a mass of spermatocysts which contain many Sertoli cells, each being associated with a clone of germ cells. In contrast, in teleosts, the cysts are confined to large elongated structures limited by a basement membrane. These structures are either lobules originating under the albuginea or tubules which, in contrast to those of mammals, are anastomosed. In the lobules, the spermatocysts start to develop at the blind end of the lobules and migrate towards the efferent system, whereas in the tubules, the spermatocysts are located against the basement membrane, all along the tubules and do not migrate. In elasmobranchs, unlike teleosts, Leydig cells are either absent from the interstitial tissue or rare and undifferentiated and their role in steroid production is at best marginal. While many studies have focused on topographical and functional interactions between the diverse cell types present in mammalian testis, only a few studies have brought particular attention to these aspects in fish. In fish, like in mammals, testicular cell-cell interactions are based on structural elements and chemical factors. Occasionally, various adhering junctions have been observed, essentially in teleosts, between Sertoli cells, between Sertoli cells and germ cells, between germ cells themselves, and interstitial cells. Furthermore, in some teleost species, using horseradish peroxidase or lanthanum salts, the presence of tight junctions between Sertoli cells has been correlated to the occurrence of a Sertoli barrier. In these species, the barrier develops after meiosis so that only haploid germ cells are shielded from the vascular system. In fish, recent development of techniques which enable the preparation and in vitro culture of enriched populations of testicular cells and of spermatocysts, has allowed investigations on functional aspects of cell-cell interactions. In particular, data have been obtained, in the trout, on the control of spermatogonia proliferation by Sertoli cell-conditioned media and, in the dogfish, on the steroidogenic activity of Sertoli cells, in relation to the differentiation stage of the associated germ cells. Furthermore information exists, in the trout, showing that intratubular macrophages may participate in the re-initiation of spermatogonial proliferation. In conclusion, the cytoarchitecture of fish testis, as compared to that of mammals, presents original features which provide unique opportunities to develop fruitful studies for a better understanding of the complex control mechanisms underlying testicular function in vertebrates.


Microsc Res Tech


Loir M,Sourdaine P,Mendis-Handagama SM,Jégou B




Has Abstract


1995-12-15 00:00:00












  • Microscopic techniques used for the identification of medicinal plants: A case study of Senna.

    abstract:BACKGROUND:The use of quality control tool for adulteration of Senna (Cassia aungustifolia) a pharmaceutically very important. They were used for multiple health disorders such as constipation, indigestion, epilepsy, asthma, piles, migraine, and heart problems. Two different species of same family or same genus used co...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Shaheen S,Jaffer M,Khalid S,Khan MA,Hussain K,Butt MM,Rauf Siddiqui A,Ashfaq M,Ahmad M,Zafar M,Khan F

    更新日期:2019-10-01 00:00:00

  • Image processing techniques in a preliminary morphometric characterization of corneal epithelium by scanning electron microscopy.

    abstract::Several processing techniques of digital images allowed us to quantify the percentage of cell surface covered by microprojections (microvilli or microplicae) (SCM), the adhesion between epithelial cells by the parameter intercellular junctions (IJ), the size (cell area), shape (cell shape) and shade (cell shade) of ce...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Julio G,Merindano D,Canals M,Ralló M

    更新日期:2010-10-01 00:00:00

  • Palyno-morphological investigations of subtropical endangered flora of Capparidaceae through light and scanning electron microscopy.

    abstract::The research was performed to investigate pollen morphology of endangered species of Capparidaceae in subtropical regions of Pakistan. The distinguishing characters were investigated by using light microscope and scanning electron microscope. Palynological study is comprised of pollen shape, pollen type, exine sculptu...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Mir S,Ahmad M,Khan MA,Zafar M,Jahan S,Sultana S,Jabeen M,Majeed S

    更新日期:2019-09-01 00:00:00

  • Capsaicin-sensitive nerve fibers: a potential extra-ACTH mechanism participating in adrenal regeneration in rats.

    abstract::Pituitary-derived factors, including ACTH, have been widely implicated in initiating adrenal regeneration. However, recent work has demonstrated that adrenal regeneration is also modulated by adrenal nerves that extensively reinnervate the regenerating adrenal. Moreover, transection of the splanchnic nerve removes sen...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Ulrich-Lai YM,Fraticelli AI,Engeland WC

    更新日期:2003-06-15 00:00:00

  • Effect of Aloe vera application on the content and molecular arrangement of glycosaminoglycans during calcaneal tendon healing.

    abstract::Although several treatments for tendon lesions have been proposed, successful tendon repair remains a great challenge for orthopedics, especially considering the high incidence of re-rupture of injured tendons. Our aim was to evaluate the pharmacological potential of Aloe vera on the content and arrangement of glycosa...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Aro AA,Esquisatto MA,Nishan U,Perez MO,Rodrigues RA,Foglio MA,Carvalho JE,Gomes L,Vidal Bde C,Pimentel ER

    更新日期:2014-12-01 00:00:00

  • Ultrastructural study of apoptotic U937 [corrected] cells treated with different pro-apoptotic substances.

    abstract::Human monocytic leukemia U937 cells readily undergo apoptosis when exposed to various stimuli, including inhibition of protein synthesis, oxidative stress, antitumoral agents, etc. The sequential, step-by-step morphological changes in U937 cells that occur during the apoptotic program are largely determined by the act...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Abbro L,Lanubile R,Dini L

    更新日期:2004-05-01 00:00:00

  • Quantitative junctional permeability measurements using the confocal microscope.

    abstract::This paper describes the use of a photobleach method and confocal microscopy to compare the cell-to-cell transfer rate of 5,6 carboxyfluorescein in dissociated embryonic chick lens cells with those in the anterior epithelium of the whole embryonic chick lens. The average cell-to-cell transfer rates obtained were 7.9 x...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Miller A

    更新日期:1995-08-01 00:00:00

  • Structure and turnover of junctional complexes between principal cells of the rat epididymis.

    abstract::The epididymal junctional complex between adjacent principal cells is composed of apically located gap, adherens and tight junctions. Tight junctions between adjacent epithelial cells lead to the formation of the blood-epididymal barrier. The objectives of this study were to examine the structure of the epididymal jun...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审


    authors: Cyr DG,Robaire B,Hermo L

    更新日期:1995-01-01 00:00:00

  • Confocal microscopy of thick sections from acrylamide gel embedded embryos.

    abstract::The preparation of optically clear, thick sections of fragile embryonic tissues greatly aids the power of confocal scanning laser microscopy in imaging three-dimensional structures. We report here conditions for embedding, sectioning, and staining embryos in polyacrylamide gels for a variety of confocal imaging techni...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Germroth PG,Gourdie RG,Thompson RP

    更新日期:1995-04-15 00:00:00

  • Femtosecond electron diffraction: direct probe of ultrafast structural dynamics in metal films.

    abstract::Femtosecond electron diffraction is a rapidly advancing technique that holds a great promise for studying ultrafast structural dynamics in phase transitions, chemical reactions, and function of biological molecules at the atomic time and length scales. In this paper, we summarize our development of a tabletop femtosec...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Nie S,Wang X,Li J,Clinite R,Cao J

    更新日期:2009-03-01 00:00:00

  • The microcomputer and image analysis in diagnostic pathology.

    abstract::This paper presents a snapshot view of the influence and direction of microcomputer technology for image analysis techniques in diagnostic pathology. Microcomputers have had considerable impact in bringing image analysis to wider application. Semi-automated tracing techniques are a simple means of providing objective ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审


    authors: Jarvis LR

    更新日期:1992-06-01 00:00:00

  • Mechanisms and microstructures of 2A97 Al-Li alloy under the hot forming with synchronous quenching process.

    abstract::Aluminum-lithium alloy is regarded as the most promising light material in the aircraft and aerospace industries. For the production of complex and high-precision parts, the hot forming with synchronous quenching (HFSQ) process has become an effective and attractive forming method. In order to achieve the performance ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Zhang P,Chen MH,Chen W,Zhang SH,Xu Y

    更新日期:2021-02-01 00:00:00

  • Microimaging FTIR of head and neck tumors. IV.

    abstract::On continuing our studies on head and neck neoplasia, specimens from salivary gland tumors have been explored by using infrared microimaging spectroscopy to discern healthy from neoplastic tissues. Samples with Warthin tumor, epithelial displasia, marginal B-cell lymphoma, low-grade adenocarcinoma, and adenoid cystic ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Conti C,Ferraris P,Garavaglia M,Giorgini E,Rubini C,Sabbatini S,Tosi G

    更新日期:2009-02-01 00:00:00

  • Deposition of lead and cadmium released by cigarette smoke in dental structures and resin composite.

    abstract::Cigarette smoke is a significant source of cadmium, lead, and toxic elements, which are absorbed into the human organism. In this context, the aim of this study was to investigate in vitro the presence of toxic elements, cadmium, and lead deriving from cigarette smoke in the resin composite, dentine, and dental enamel...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Takeuchi CY,Corrêa-Afonso AM,Pedrazzi H,Dinelli W,Palma-Dibb RG

    更新日期:2011-03-01 00:00:00

  • Effects of ultraviolet B radiation on physicochemical properties of human hair shaft.

    abstract::Changes in physicochemical properties of the human hair shaft induced by UVB during the summer climate were investigated. Using atomic force microscopy and thermogravimetric analyzer measurements, we investigated the changes in hair shaft physical properties including morphology, roughness, and water content. The chan...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Kim KS,Shin MK,Park HK

    更新日期:2012-07-01 00:00:00

  • Surface ultrastructure of the gill filaments and the secondary lamellae of the catfish, Rita rita, and the carp, Cirrhinus mrigala.

    abstract::Surface ultrastructures of gill filaments and secondary lamellae of Rita rita and Cirrhinus mrigala, inhabiting different ecological habitat, were investigated to unravel adaptive modifications. R. rita is a sluggish, bottom dwelling carnivorous catfish, which inhabits regions of river with accumulations of dirty wate...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Kumari U,Mittal S,Mittal AK

    更新日期:2012-04-01 00:00:00

  • In situ hybridization studies confirming recent findings of the existence of a local nonneuronal catecholamine production in human patellar tendinosis.

    abstract::We have in recent studies presented unexpected immunohistochemical evidence favoring the existence of a local production of catecholamines, and an occurrence of adrenergic receptors on the tendon cells (tenocytes), in the human patellar tendon. This was particularly noticed for tendons from patients suffering from ten...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Danielson P,Alfredson H,Forsgren S

    更新日期:2007-10-01 00:00:00

  • Application of photoconversion technique for correlated confocal and ultrastructural studies in organotypic slice cultures.

    abstract::Photoconversion of fluorescent staining into stable diaminobenzidine (DAB) precipitate is widely used for neuroanatomical and developmental studies. An important advantage of the approach is to make correlations between light and electron microscopy analyses possible, the DAB reaction product formed during photoconver...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Nikonenko I,Boda B,Alberi S,Muller D

    更新日期:2005-10-01 00:00:00

  • Nanoanalysis by a high-resolution energy filtering transmission electron microscope.

    abstract::An energy-filtering transmission electron microscope with 300 kV acceleration voltage was developed and the spatial resolution of elemental distribution images was improved. Observing oxygen monolayers in Al(11)O(3)N(9), it was shown that the actual resolution attained is up to 0.5 nm. Surface plasmon loss images of s...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Mitome M,Bando Y,Golberg D,Kurashima K,Okura Y,Kaneyama T,Naruse M,Honda Y

    更新日期:2004-02-15 00:00:00

  • Müllerian inhibiting substance: new perspectives and future directions.

    abstract::MIS, as a differentiate and antiproliferative agent, is precisely regulated, for example, at the transcriptional level by such transacting factors as SRY, and posttranslationally by testosterone. Processing of MIS most likely requires an as yet unknown in vivo protease which probably serves to control cleavage of MIS ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审


    authors: Catlin EA,MacLaughlin DT,Donahoe PK

    更新日期:1993-06-01 00:00:00

  • Morphometric study of adipocytes on breast cancer by means of photonic microscopy and image analysis.

    abstract::Worldwide, breast cancer (BrCa) is currently the leading cause of deaths associated to malignant lesions in adult women. Given that some studies have mentioned that peritumoral adipocytes may contribute to breast carcinogenesis, present work sought to quantitative evaluate the morphometry of these cells in a group of ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Suárez-Nájera LE,Chanona-Pérez JJ,Valdivia-Flores A,Marrero-Rodríguez D,Salcedo-Vargas M,García-Ruiz DI,Castro-Reyes MA

    更新日期:2018-02-01 00:00:00

  • Mitochondrial alterations and autofluorescent conversion of Candida albicans induced by histatins.

    abstract::The mechanism of the candidacidal activity of histatins 3 and 5 (Hst) is still a matter of debate. Previous studies have indicated that Hst induce cell permeabilization, generation of reactive oxygen species (ROS) by mitochondria, inhibition of the respiratory chain, and energy-dependent cytotoxic release of ATP. On t...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Diaz G,Polonelli L,Conti S,Messana I,Cabras T,Putzolu M,Falchi AM,Fadda ME,Cosentino S,Isola R

    更新日期:2005-04-01 00:00:00

  • Histomicroscopy and normal anatomy of the adult killifish Aphanius hormuzensis (Teleostei; Aphaniidae) from the Persian Gulf coastal environment.

    abstract::The histomicroscopy and normal anatomy of the major body organ systems were investigated in the adult killifish, Aphanius hormuzensis using histological examination, X-ray imaging, double staining, light microscopy and scanning electron microscopy (SEM). Based on the histomicroscopic observations, the kidney, liver an...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Motamedi M,Shamsaldini F,Teimori A,Hesni MA

    更新日期:2019-04-01 00:00:00

  • Identification of ages and determination of paeoniflorin in roots of Paeonia lactiflora Pall. From four producing areas based on growth rings.

    abstract::Growth rings were used to determine the root age of medicinal Paeonia lactiflora from four producing areas, and their corresponding paeoniflorin content were measured based on the identification of ages. Different P. lactiflora root samples of different ages were collected from the four major growing areas in China: B...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Zha LP,Cheng ME,Peng HS

    更新日期:2012-09-01 00:00:00

  • Investigation of mineral content of root canal dentin after the application of various antibiotic paste using energy-dispersive X-ray detector.

    abstract::The purpose of this study was to evaluate mineral content of root canal dentin after treatment with different antibiotic pastes including the mixture of metronidazole, ciprofloxacin, doxycycline, cefaclor, amoxicillin, or minocycline. Fifty extracted maxillary canine teeth were randomly divided into five groups (n = 1...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Simsek H,Coruh M,Cakici F,Fundaoglu Kucukekenci F,Gurbuz T,Cakici EB

    更新日期:2019-02-01 00:00:00

  • Morphology and histochemistry of the intramandibular glands in Attini and Ponerini (Hymenoptera, Formicidae) species.

    abstract::An understanding of the internal morphology of the ant mandible is important in explaining the relationship between the exocrine system and the behavioral and phylogenetic characteristics of different subfamilies of Formicidae. In this study, we investigated the occurrence of intramandibular glands in ants from the Po...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Martins LC,Serrão JE

    更新日期:2011-08-01 00:00:00

  • Circadian organization and the role of the pineal in birds.

    abstract::All organisms exhibit significant daily rhythms in a myriad of functions from molecular levels to the level of the whole organism. Significantly, most of these rhythms will persist under constant conditions, showing that they are driven by an internal circadian clock. In birds the circadian system is composed of sever...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审


    authors: Underwood H,Steele CT,Zivkovic B

    更新日期:2001-04-01 00:00:00

  • Peripheral and central structures involved in insect gustation.

    abstract::Studies in insect gustation have a long history in general physiology, particularly with work on fly labellar and tarsal sensilla and in the general field of insect-plant interactions, where work on immature Lepidoptera and chrysomelid beetles has been prominent. Much more emphasis has been placed on the physiological...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审


    authors: Mitchell BK,Itagaki H,Rivet MP

    更新日期:1999-12-15 00:00:00

  • Microporosity and polymerization contraction as function of depth in dental resin cements by X-ray computed microtomography.

    abstract::This research aimed to obtain the depth dependence of polymerization contraction and microporosity from irradiated dental resin cements by X-ray computed microtomography (μCT). Samples (n = 5) of commercial Relyx U200 (RU) and AllCem Core (AC) dual-cure resin cements were injected in a cylindrical Teflon sampler (25 m...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Gheller R,Burey A,Vicentin BLS,Dos Reis PJ,Appoloni CR,Garbelini CCD,Hoeppner MG

    更新日期:2020-06-01 00:00:00

  • Brain tumor detection and classification: A framework of marker-based watershed algorithm and multilevel priority features selection.

    abstract::Brain tumor identification using magnetic resonance images (MRI) is an important research domain in the field of medical imaging. Use of computerized techniques helps the doctors for the diagnosis and treatment against brain cancer. In this article, an automated system is developed for tumor extraction and classificat...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Khan MA,Lali IU,Rehman A,Ishaq M,Sharif M,Saba T,Zahoor S,Akram T

    更新日期:2019-06-01 00:00:00