Abstract:
:The effectiveness of different microscopy techniques for measuring the dimensions of ultimate fibers from harakeke (Phormium tenax, New Zealand flax) was investigated using a factorial experimental design. Constant variables were geographical location, location of specimens along the leaf, season (winter), individual plant, a fourth leaf from a north-facing fan, age of plant, and cultivars (two). Experimental variables were microscopy techniques and measurement axis. Measurements of width and length of harakeke ultimate fibers depended on the microscopic preparation/technique used as well as the cultivar examined. The best methods were (i) transverse sections of leaf specimens 4 microm thick, embedded in Paraplast and observed using light microscopy, and (ii) nonfixed ultimate fibers observed using scanning electron microscopy.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Cruthers N,Carr D,Niven B,Girvan E,Laing Rdoi
10.1002/jemt.20206keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
260-4issue
5eissn
1059-910Xissn
1097-0029journal_volume
67pub_type
杂志文章abstract::We observed the corrosion casts of the Wistar rats' pancreatic ducts with scanning electron microscopy (SEM), and their conventionally fixed pancreatic tissue with SEM and transmission electron microscopy (TEM). These findings revealed the following facts about the three-dimensional structure of pancreatic duct. (1) T...
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