Abstract:
:Using the optical methods described, phase specimens can be observed with a modified light microscope in enhanced clarity, purified from typical artifacts which are apparent in standard phase contrast illumination. In particular, haloing and shade-off are absent, lateral and vertical resolution are maximized and the image quality remains constant even in problematic preparations which cannot be well examined in normal phase contrast, such as specimens beyond a critical thickness or covered by obliquely situated cover slips. The background brightness and thus the range of contrast can be continuously modulated and specimens can be illuminated in concentric-peripheral, axial or paraxial light. Additional contrast effects can be achieved by spectral color separation. Normal glass or mirror lenses can be used; they do not need to be fitted with a phase plate or a phase ring. The methods described should be of general interest for all disciplines using phase microscopy.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Piper T,Piper Jdoi
10.1002/jemt.22265subject
Has Abstractpub_date
2013-10-01 00:00:00pages
1050-6issue
10eissn
1059-910Xissn
1097-0029journal_volume
76pub_type
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