Phase contrast without phase plates and phase rings--optical solutions for improved imaging of phase structures.

Abstract:

:Using the optical methods described, phase specimens can be observed with a modified light microscope in enhanced clarity, purified from typical artifacts which are apparent in standard phase contrast illumination. In particular, haloing and shade-off are absent, lateral and vertical resolution are maximized and the image quality remains constant even in problematic preparations which cannot be well examined in normal phase contrast, such as specimens beyond a critical thickness or covered by obliquely situated cover slips. The background brightness and thus the range of contrast can be continuously modulated and specimens can be illuminated in concentric-peripheral, axial or paraxial light. Additional contrast effects can be achieved by spectral color separation. Normal glass or mirror lenses can be used; they do not need to be fitted with a phase plate or a phase ring. The methods described should be of general interest for all disciplines using phase microscopy.

journal_name

Microsc Res Tech

authors

Piper T,Piper J

doi

10.1002/jemt.22265

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

1050-6

issue

10

eissn

1059-910X

issn

1097-0029

journal_volume

76

pub_type

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