Abstract:
:The intravacuolar network inside the parasitophorous vacuole of Toxoplasma gondii consists of an intricate system of membrane-limited tubules of uncertain role in parasite development. We propose that it is an important structural support to the maintenance of the parasites in the characteristic rosette arrangement of parasites inside the vacuole, rather than being associated with the nutrient acquisition from the host cell, as previously suggested. We based our assumptions on observations made by field emission scanning electron microscopy of an epithelial cell line (LLCMK2) infected at various time intervals. Scraping the surface of infected monolayers with Scotch tape exposed the inner organization of the parasitophorous vacuole. Ultrathin sections and freeze-fracture replicas of analogous samples were correlated with field emission observations and added new data on tubular membranes and general organization of the parasitophorous vacuole.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Magno RC,Lemgruber L,Vommaro RC,De Souza W,Attias Mdoi
10.1002/jemt.20182keywords:
subject
Has Abstractpub_date
2005-05-01 00:00:00pages
45-52issue
1eissn
1059-910Xissn
1097-0029journal_volume
67pub_type
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