Appraisal of SIMS applicability to boron studies in plants.

Abstract:

:In the search for a new methodological approach applicable to the determination of the still poorly known primary role of boron in plant physiology, we have undertaken to appraise the potential of the SIMS method for the analytical imaging of the boron isotopes, (10)B and (11)B, at physiological concentrations in plants. With our own, CAMECA IMS4F SIMS ion analyser, and using O(2)(+) as primary ions for the detection of B(+) (plus (12)C(+) and (40)Ca(+)) secondary ions, we have been able to map quantitatively the two boron isotopes in control and boron-enriched plants, to evaluate boron concentrations at the level of individual cells and to determine boron isotopic ratios. This provides the opportunity to carry out the simultaneous labeling and imaging of boron, using enrichment with the stable isotopes, (10)B and (11)B. The method has also the potential for the simultaneous, quantitative detection of the boron isotopes and of the borate-binding sites in plant cells.

journal_name

Microsc Res Tech

authors

Dérue C,Gibouin D,Verdus MC,Lefebvre F,Demarty M,Ripoll C,Thellier M

doi

10.1002/jemt.10125

keywords:

subject

Has Abstract

pub_date

2002-07-15 00:00:00

pages

104-10

issue

2

eissn

1059-910X

issn

1097-0029

journal_volume

58

pub_type

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