In situ hybridization in HIV research.

Abstract:

:In situ hybridization (ISH) for HIV is an arduous, demanding means of detecting viral genetic material in cells and tissues. Good ISH requires broad technical skills and devotion to controls for every step of the process as well as a critical eye when interpreting results. ISH may be used to detect HIV in three ways: by hybridizing to viral RNA, by hybridizing to proviral mRNA being produced for virion packaging, and by hybridizing to proviral DNA in the cytoplasm or integrated in the nucleus of an infected cell. Here we discuss the technical considerations involved and the problems encountered in using ISH to study the pathobiology of HIV infection.

journal_name

Microsc Res Tech

authors

Fox CH,Cottler-Fox M

doi

10.1002/jemt.1070250111

subject

Has Abstract

pub_date

1993-05-01 00:00:00

pages

78-84

issue

1

eissn

1059-910X

issn

1097-0029

journal_volume

25

pub_type

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