Abstract:
:In situ hybridization (ISH) for HIV is an arduous, demanding means of detecting viral genetic material in cells and tissues. Good ISH requires broad technical skills and devotion to controls for every step of the process as well as a critical eye when interpreting results. ISH may be used to detect HIV in three ways: by hybridizing to viral RNA, by hybridizing to proviral mRNA being produced for virion packaging, and by hybridizing to proviral DNA in the cytoplasm or integrated in the nucleus of an infected cell. Here we discuss the technical considerations involved and the problems encountered in using ISH to study the pathobiology of HIV infection.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Fox CH,Cottler-Fox Mdoi
10.1002/jemt.1070250111subject
Has Abstractpub_date
1993-05-01 00:00:00pages
78-84issue
1eissn
1059-910Xissn
1097-0029journal_volume
25pub_type
杂志文章,评审abstract::Ascidian embryos are useful for examining how events that occur during fertilization and cleavage affect gastrulation because they gastrulate early in development, during the seventh cleavage. In ascidians, both dorsal-ventral and anterior-posterior axes are determined before first cleavage. The dorsal-ventral axis is...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070260403
更新日期:1993-11-01 00:00:00
abstract::Oligodendrocytes (OL) play a significant physiological role in the central nervous system by creating the myelin sheath that allows for the efficient conduction of nerve impulses. Therefore, it is important to understand which signalling cascades define the proliferation, differentiation, survival, and myelin formatio...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1052
更新日期:2001-03-15 00:00:00
abstract::Micromass cultures represent a convenient means of studying chondrocyte physiology in the context of a tridimensional culture model. In this study, we present the first ultrastructural analysis of the distribution and organization of the extracellular components in micromasses in comparison with their cartilaginous co...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20210
更新日期:2005-08-15 00:00:00
abstract::In vivo and in vitro multiphoton imaging was used to perform high resolution optical sectioning of human hair by nonlinear excitation of endogenous as well as exogenous fluorophores. Multiphoton fluorescence lifetime imaging (FLIM) based on time-resolved single photon counting and near-infrared femtosecond laser pulse...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20395
更新日期:2007-02-01 00:00:00
abstract::It is increasingly recognized that neuroepithelial endocrine cells and organoid clusters of these cells termed neuroepithelial bodies are widely distributed in the respiratory tract of vertebrates. This review focuses on the neuroepithelial endocrine system in the airway epithelium of mammals according to observations...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970401)37:1<31::AID-JEMT
更新日期:1997-04-01 00:00:00
abstract::One of the lessons learned from studying the nervous systems of phylogenetically distant species is that many features are conserved. Indeed, aminergic neurons in invertebrate and vertebrate systems share a multitude of common characteristics. In this review, the varied roles of serotonin, octopamine, dopamine, and hi...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19990115/01)44:2/3<105::AI
更新日期:1999-01-15 00:00:00
abstract::The aim of this study was to make a comparative evaluation of the surface topography of the cervical third and tip of four different rotary systems, before and after being used 12 times, in resin blocks with simulated root canals with standardized 45° curvatures, and analyzed by scanning electronic microscopy (SEM). A...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22139
更新日期:2013-01-01 00:00:00
abstract::This paper describes four investigations of the olfactory mucosa of the brown trout: 1) the ultrastructure of the olfactory mucosa as revealed by scanning (SEM), conventional transmission (TEM), and high voltage (HVEM) electron microscopy; 2) light and electron-microscopic investigations of retrograde transport of the...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070230104
更新日期:1992-10-01 00:00:00
abstract::During late anaphase and telophase, animal cells develop a bundle of antiparallel, interdigitating microtubules between the two daughter nuclei. Recent data indicate that this structure, called the central spindle, plays an essential role during cytokinesis. Studies in Drosophila and on vertebrate cells strongly sugge...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(20000415)49:2<202::AID-JEM
更新日期:2000-04-15 00:00:00
abstract::Actin, though highly conserved, exhibits a myriad of diverse functions, most of which ultimately depend on its intrinsic ability to rapidly assemble and disassemble filamentous structures. Many organisms synthesize multiple actin isoforms even within the same cell. Tissue-specific expression patterns and tight develop...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19991001)47:1<38::AID-JEMT
更新日期:1999-10-01 00:00:00
abstract::Progressive multifocal leukoencephalopathy is an important viral opportunistic infection of oligodendrocytes leading to direct demyelination. Virus is likely disseminated to the brain via the blood. However, the timing of that dissemination with relationship to clinical disease is unknown. Important clues about viral ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070320405
更新日期:1995-11-01 00:00:00
abstract::The molecular basis of hearing is less well understood than many other senses. However, recent studies in Drosophila have provided some important steps towards a molecular understanding of hearing. In this report, we summarize these findings and their implications on the relationship between hearing and touch. In Dros...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20053
更新日期:2004-04-15 00:00:00
abstract::In mammals, the neurohypophysial hormone oxytocin (OT) is released into the bloodstream during labor and lactation to promote uterine contraction and milk ejection, respectively. Electrophysiological studies have established that OT neurons fire in brief, synchronized bursts during this release. During pregnancy and l...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10019
更新日期:2002-01-15 00:00:00
abstract::Around 30 pollen samples, 16 families and 25 genera that were gathered, identified and preserved from 6 different localities of District Sheikhupura. Light and scanning electron microscopy was done for all collected flora. Out of the total samples, 43% specie was identified as herbs, 23% as shrubs, and 33% were identi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23656
更新日期:2020-12-31 00:00:00
abstract::We report for the first time the presence of cluster crystals of calcium oxalate within the glandular trichomes and oil bodies in the mesophyll for Baccharis species. Moreover, the comparative leaf anatomy and micro-morphology of six species of Baccharis, namely B. illinita, B. microdonta, B. pauciflosculosa, B. punct...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23045
更新日期:2018-08-01 00:00:00
abstract::This work provides examples of some of the imaging capabilities of environmental scanning electron microscopy applied to easily charged samples relevant to particle analysis. Environmental SEM (also referred to as high pressure or low vacuum SEM) can address uncoated samples that are known to be difficult to image. Mo...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070250507
更新日期:1993-08-01 00:00:00
abstract::The visual system of insects provides an excellent model to study processes of transduction and transmission of photic information, synaptogenesis, synaptic plasticity, and wiring between photoreceptors and their visual interneurons in the optic lobe. This review describes synaptic contacts between photoreceptors and ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10141
更新日期:2002-08-15 00:00:00
abstract::The applicability of the binomial frequency distribution is outlined for the analysis of the evolution nanoscale atomic clustering of dilute solute in an alloy subject to thermal ageing in 3D atom probe data. The conventional chi(2) statistics and significance testing are demonstrated to be inappropriate for compariso...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20582
更新日期:2008-07-01 00:00:00
abstract::We have summarized current knowledge on the aging of the immune system in three vertebrate groups: fish, amphibians and birds. Available data are few due to difficulties in studying ageing in natural populations and in accurately determining age. In all vertebrates, the most obvious evidence of the senescence of lymph...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10409
更新日期:2003-12-15 00:00:00
abstract::This bibliography is compiled to assist in locating papers related to the application of scanning electron microscopy (SEM) to cerebrospinal-fluid-contacting surfaces in vertebrates. The use of SEM by neuroscientists has continued apace since the publication of the first bibliography in 1980. SEM studies now include m...
journal_title:Microscopy research and technique
pub_type:
doi:10.1002/(SICI)1097-0029(19980415)41:2<158::AID-JEM
更新日期:1998-04-15 00:00:00
abstract::This study was designed to investigate patterns of fibrils organization in histochemically stained otoconia. Transmission electron microscope and video imaging were used. These data indicate that otoconia of the chick (Gallus domesticus) inner ear may have central cores in vivo. The data also show that the ultrastruct...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070250406
更新日期:1993-07-01 00:00:00
abstract::A major challenge when performing scanning electron microscopy and X-ray analysis on many ceramic materials is their electrical insulation properties, which leads to buildup of the surface charge and reduced contrast in the secondary electron image. A new procedure was established to quantitatively determine the neutr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23118
更新日期:2018-10-01 00:00:00
abstract::Previous studies reported that intrageneric relationships of genus Veratrum of family Melanthiaceae are controversial and hard to delimit. Therefore, we observed the pollen morphological features of six species in the genus Veratrum in detail using both light microscopy and scanning electron microscopy and investigate...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23479
更新日期:2020-08-01 00:00:00
abstract::Macro and microdissection methods, conventional histology and immunohistochemical procedures were used to investigate the nasal cavity and turbinate complex in fetal and adult sheep, with special attention to the ethmoturbinates, the vestibular mucosa, and the septal mucosa posterior to the vomeronasal organ. The ecto...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22436
更新日期:2014-12-01 00:00:00
abstract::Calorie restriction (CR) is known to delay the aging process in rodents and is postulated to act by decreasing free radical generation and increasing antioxidant enzyme activity. The present study was designed to investigate the effect of CR and age on oxidative stress-induced apoptosis and associated changes in the l...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10206
更新日期:2002-11-15 00:00:00
abstract::During the emergence of multicellular organisms, molecular mechanisms evolved to allow maintenance of anatomical integrity and self-recognition. We propose that carbohydrates from proteoglycans, as the most peripheral cell surface, and matrix molecules might have provided these key adhesion and recognition functions. ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19990215)44:4<304::AID-JEM
更新日期:1999-02-15 00:00:00
abstract::Samples of polyglycolic acid PGA/polycaprolactone PCL copolymer absorbable suture materials, which is called Poliglecaprone PGC copolymer suture, were thermally treated by two different processes. The first method is the direct heat exposure, from 20 to 38 °C, in the surrounding medium. Which is an environmental study...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22957
更新日期:2018-01-01 00:00:00
abstract::Although the Geographical Information System (GIS), which integrates computerized drawing computer assisted design (CAD) and relational databases (data base management system (DBMS)), is best known for applications in geographical and planning cartography, it can also use many kinds of information concerning the terri...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22008
更新日期:2012-07-01 00:00:00
abstract::Tapping mode atomic force microscopy (AFM) provides phase images in addition to height and amplitude images. Although the behavior of tapping mode AFM has been investigated using mathematical modeling, comprehensive understanding of the behavior of tapping mode AFM still poses a significant challenge to the AFM commun...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22558
更新日期:2015-10-01 00:00:00
abstract::We present de novo studies and review published efforts from our laboratory, spanning 12 years (from 1988 to 2000), where we have used ultrastructural approaches to study the functional anatomy of the microvasculature in man and animals in health and disease. These efforts have defined a new endothelial cell organelle...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10087
更新日期:2002-06-01 00:00:00