Multiphoton fluorescence lifetime imaging of human hair.

Abstract:

:In vivo and in vitro multiphoton imaging was used to perform high resolution optical sectioning of human hair by nonlinear excitation of endogenous as well as exogenous fluorophores. Multiphoton fluorescence lifetime imaging (FLIM) based on time-resolved single photon counting and near-infrared femtosecond laser pulse excitation was employed to analyze the various fluorescent hair components. Time-resolved multiphoton imaging of intratissue pigments has the potential (i) to identify endogenous keratin and melanin, (ii) to obtain information on intrahair dye accumulation, (iii) to study bleaching effects, and (iv) to monitor the intratissue diffusion of pharmaceutical and cosmetical components along hair shafts.

journal_name

Microsc Res Tech

authors

Ehlers A,Riemann I,Stark M,König K

doi

10.1002/jemt.20395

subject

Has Abstract

pub_date

2007-02-01 00:00:00

pages

154-61

issue

2

eissn

1059-910X

issn

1097-0029

journal_volume

70

pub_type

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