A novel method for transmission electron microscopy study of cytoplasmic fragments from preimplantation human embryos.

Abstract:

:Transmission electron microscopy (TEM) is the main tool for exploring the intracellular damage and organelle distribution. The cause of producing embryo cytoplsamic fragmentation is not completely understood. Since the fragments have detrimental effects on embryo development, the ultrastructural analysis of fragments may play an important role in fragmentation etiology and in embryo development as well. There are no studies regarding the ultrastructure of fragments in transferable embryos, because the preparation for TEM is not vital and embryos are discarded inevitably. This study aims to introduce a new method for ultrastructural evaluation of fragments without damaging the human cleaving embryos. Microsc. Res. Tech. 79:459-462, 2016. © 2016 Wiley Periodicals, Inc.

journal_name

Microsc Res Tech

authors

Halvaei I,Khalili MA,Nottola SA

doi

10.1002/jemt.22657

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

459-62

issue

6

eissn

1059-910X

issn

1097-0029

journal_volume

79

pub_type

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