Characterization of elemental distribution across human dentin-enamel junction by scanning electron microscopy with energy-dispersive X-ray spectroscopy.

Abstract:

:The human dentin-enamel junction (DEJ) is a natural junction that unites two dissimilar mineralized tissues in the human tooth: enamel and dentin. DEJ plays a critical role in maintaining structural and functional integrity of the tooth. However, its structure, chemical composition and function remain unclear and controversial. Systematic investigation of elemental distribution across human DEJ is still lacking in the literature. This study aimed to investigate the elemental distributions of Ca, P, O, C, N, Na, and Mg across the DEJ of human teeth using scanning electron microscope with energy dispersive spectroscopy of X-ray. The results revealed abrupt changes in the distributions of six elements (C, N, Ca, P, Na, and Mg) across the DEJ. Specifically, the four mineral elements showed similar level of change in distribution, with Ca, P, Na decreasing while Mg increasing by 21%-25% from enamel to dentin side of the DEJ. The two organic elements C and N showed much larger changes in distribution, with C increasing by ~150% and N increasing by ~270% from enamel to dentin side of the DEJ. The slope of the distribution curves across the DEJ was estimated to be ~2 μm in width and coincided with the phase intermixing of the micro-scallop structure of the DEJ.

journal_name

Microsc Res Tech

authors

Wang R,Zhao D,Wang Y

doi

10.1002/jemt.23648

subject

Has Abstract

pub_date

2020-11-19 00:00:00

eissn

1059-910X

issn

1097-0029

pub_type

杂志文章
  • The transformation of phytolith morphology as the result of their exposure to high temperature.

    abstract::Phytoliths are an important component for interpreting the ancient botanical record. However, phytoliths can be altered through heating, either as the result of such activities as firing ceramics, clay molds use for casting metal or in hearths. Phytoliths can also be altered through heating as the result of creating c...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22004

    authors: Wu Y,Wang C,Hill DV

    更新日期:2012-07-01 00:00:00

  • Preparation of smallest microparticles of poly-D,L-lactide by modified precipitation method: influence of the process parameters.

    abstract::Biodegradable microspheres such as those made of poly-D,L-lactide (PDLLA) are widely investigated delivery systems for drugs or antigens. The aim of this study was to examine experimental conditions in order to produce PDLLA microspheres with the best properties for controlled and sustained drug delivery by the modifi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20529

    authors: Jovanović I,Jordović B,Petković M,Ignjatović N,Uskoković D

    更新日期:2008-02-01 00:00:00

  • High-contrast optical microscopy of graphene sheets.

    abstract::In the way of making graphene an industry-friendly material, it must be mass-produced with high-quality and reduced cost over large areas. Assisted by machine-learning techniques, rapid, nondestructive and accurate determination of large graphene sheets on SiO2 /Si substrates has been made possible in recent years by ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23505

    authors: Rashidian Vaziri MR

    更新日期:2020-09-01 00:00:00

  • Fossil record of echinoderm regeneration with special regard to crinoids.

    abstract::Regeneration of skeletal parts of fossil echinoderms is reviewed. Among regenerations of various fossil echinoderms, those of crinoids are most common and many examples have been reported. Mesozoic and Cenozoic crinoids, the subclass Articulata, are characterized by the dominance of muscular articulations in the arms ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1186

    authors: Oji T

    更新日期:2001-12-15 00:00:00

  • Cellular relations in mouse circumvallate taste buds.

    abstract::The fine structure of the taste buds of circumvallate papillae of two strains of mice was studied by electron microscopy. Mice anesthetized with ketamine were perfused through the heart with a double aldehyde mixture in cacodylate buffer and the tissues embedded in Epon. Semi-serial sections were employed. The morphol...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070260304

    authors: Murray RG

    更新日期:1993-10-15 00:00:00

  • Morphology of the pancreatic duct system in mammals.

    abstract::The morphology of pancreatic excretory duct segments was reviewed in mammals. The fine structure of the epithelial lining was described in intercalated ducts, intra- and extralobular ducts, and in major pancreatic ducts. Morphological characteristics of the various cell types comprising to the duct epithelium were det...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19970601)37:5/6<407::AID-J

    authors: Egerbacher M,Böck P

    更新日期:1997-06-01 00:00:00

  • Authenticating and distinguishing the eight species of traditional Tibetan medicine "Meiduoluomi" by microscopic technique.

    abstract::Because of the morphological and macroscopic similarity, many species of Erigeron and Aster (Asteraceae) are confusable and usually used under the same name "Meiduoluomi" in traditional Tibetan medicine (TTM). To find an easy, quick, and reliable method to authenticate and distinguish the eight main medicinal plants o...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20725

    authors: An J,Li J,Wang JG,Zhang ZF,Chen C,Zhang H

    更新日期:2009-10-01 00:00:00

  • Dengue in the Dominican Republic: epidemiology for 2004.

    abstract::The Dominican Republic (DR) has experienced tremendous increase in the number of cases of dengue virus (DENV) reported in the past few years. There are four serotypes of DENV (1-4), and each can cause classic dengue fever (DF), dengue hemorrhagic fever (DHF), or dengue shock syndrome (DSS). DENV-1-4 are currently circ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20225

    authors: Cabrera-Batista B,Skewes-Ramm R,Fermin CD,Garry RF

    更新日期:2005-11-01 00:00:00

  • Angiogenesis of buffalo choroid plexuses: structural and immunocytochemical study.

    abstract::Mammalian choroid plexuses (CPs) are vascularized structures involved in numerous exchange processes that supply nutrients and hormones to the brain, and that remove deleterious compounds and metabolites from the brain. Studies in the adult Mediterranean buffalo have investigated the morphology of CPs using histochemi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22037

    authors: Scala G,Maruccio L

    更新日期:2012-08-01 00:00:00

  • Environmental scanning electron microscope imaging examples related to particle analysis.

    abstract::This work provides examples of some of the imaging capabilities of environmental scanning electron microscopy applied to easily charged samples relevant to particle analysis. Environmental SEM (also referred to as high pressure or low vacuum SEM) can address uncoated samples that are known to be difficult to image. Mo...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070250507

    authors: Wight SA,Zeissler CJ

    更新日期:1993-08-01 00:00:00

  • Scanning electron microscopy of central nervous system cerebrospinal-fluid-contacting surfaces: a bibliography (1963-1995).

    abstract::This bibliography is compiled to assist in locating papers related to the application of scanning electron microscopy (SEM) to cerebrospinal-fluid-contacting surfaces in vertebrates. The use of SEM by neuroscientists has continued apace since the publication of the first bibliography in 1980. SEM studies now include m...

    journal_title:Microscopy research and technique

    pub_type:

    doi:10.1002/(SICI)1097-0029(19980415)41:2<158::AID-JEM

    authors: Mitchell JA,Ham S

    更新日期:1998-04-15 00:00:00

  • Optical-sectioning improvement in two-color excitation scanning microscopy.

    abstract::We present a new beam-shaping technique for two-color excitation fluorescence microscopy. We show that by simply inserting a properly designed shaded-ring filter in the illumination beam of smaller wavelength, it is possible to improve the effective optical sectioning capacity of such microscopes by 23%. Such an impro...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20073

    authors: Ibáñez-López C,Escobar I,Saavedra G,Martínez-Corral M

    更新日期:2004-06-01 00:00:00

  • Structure, assembly, and dynamics of actin filaments in situ and in vitro.

    abstract::Actin, though highly conserved, exhibits a myriad of diverse functions, most of which ultimately depend on its intrinsic ability to rapidly assemble and disassemble filamentous structures. Many organisms synthesize multiple actin isoforms even within the same cell. Tissue-specific expression patterns and tight develop...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19991001)47:1<38::AID-JEMT

    authors: Schoenenberger CA,Steinmetz MO,Stoffler D,Mandinova A,Aebi U

    更新日期:1999-10-01 00:00:00

  • Developmental aspects of lipid and lipoprotein synthesis and secretion in human gut.

    abstract::This review article focuses on the ontogeny and the regulatory mechanisms involved in the modulation of the intracellular events governing the assembly and delivery of lipoproteins in human gut. The human fetal intestine organizes villi covered with well-differentiated enterocytes during the end of the first trimester...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(20000515)49:4<363::AID-JEM

    authors: Levy E,Ménard D

    更新日期:2000-05-15 00:00:00

  • Efficacy of different irrigant protocols and application systems on sealer penetration using a stepwise CLSM analysis.

    abstract::This study compared several irrigation protocols and application systems for sealer penetration into dentinal tubules. Single-rooted-human teeth were divided into 5 experimental groups (n = 15) and a control group (n = 5), according to final irrigation protocols: standard needle irrigation (SNI); Vibringe; Vibringe + ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22944

    authors: Aksel H,Küçükkaya Eren S,Puralı N,Serper A,Azim AA

    更新日期:2017-12-01 00:00:00

  • Effects of aerobic training, resistance training, or combined resistance-aerobic training on the left ventricular myocardium in a rat model.

    abstract::This study follows the left ventricular (LV) hypertrophy in rats undergoing aerobic training alone (A), resistance training alone (R), or combined resistance and aerobic training (RA) (usually referred as concurrent training) program. A sedentary control group (C) was included. LV remodeling was evaluated using electr...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22394

    authors: De Souza MR,Pimenta L,Pithon-Curi TC,Bucci M,Fontinele RG,De Souza RR

    更新日期:2014-09-01 00:00:00

  • Moving legs: A workflow on how to generate a flexible endopod of the 518 million-year-old Chengjiang arthropod Ercaicunia multinodosa using 3D-kinematics (Cambrian, China).

    abstract::Understanding the functional morphology and mobility of appendages of fossil animals is important for exploring ecological traits such as feeding and locomotion. Previous work on fossils from the 518 million-year-old Chengjiang biota of China was based mainly on two-dimensional information captured from the surface of...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23628

    authors: Schmidt M,Liu Y,Zhai D,Hou X,Melzer RR

    更新日期:2020-11-06 00:00:00

  • Three months of Western diet induces small intestinal mucosa alteration in TLR KO mice.

    abstract::Several studies support the role of Western-style diet (WD) in inflammatory bowel disease (IBD). Toll-like receptors/NOD-like receptors (TLRs/NLRs) are important to maintain a healthy epithelium as well as inducing inflammation. Given that dietary factors influence IBD development, that epithelial dysfunction is thoug...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22831

    authors: Sardi C,Luchini P,Emanuelli A,Giannoni A,Martini E,Manara LM,Sfondrini L,Kallikourdis M,Sommariva M,Rumio C

    更新日期:2017-06-01 00:00:00

  • Analysis of the interfacial micromorphology of adhesive systems in cavities prepared with Er,Cr:YSGG, Er:YAG laser and bur.

    abstract::The purpose of this in vitro study was to evaluate the interaction pattern of adhesive systems on laser and bur cavities. Cavities were prepared according to the following groups (n=9): (G1) conventional diamond bur (No. 1013); (G2) Er:YAG laser (250 mJ, 4 Hz, 80.6 J/cm2); (G3) Er,Cr:YSGG laser (3.5 W, 20 Hz, 61.7 J/c...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20459

    authors: Aranha AC,De Paula Eduardo C,Gutknecht N,Marques MM,Ramalho KM,Apel C

    更新日期:2007-08-01 00:00:00

  • Ultrastructure and three-dimensional study of the lymphatic stomata in the costal pleura of the rabbit.

    abstract::The aim of this report was to investigate the ultrastructure and three-dimensional organization of the pleural lymphatic stomata in the adult rabbit costal pleura by electron microscopy. A computer image processing system attached to the scanning electron microscopy was used to get statistical evaluation of the dimens...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.10388

    authors: Li YY,Li JC

    更新日期:2003-10-15 00:00:00

  • Gene expression in peripheral arterial chemoreceptors.

    abstract::The peripheral arterial chemoreceptors of the carotid body participate in the ventilatory responses to hypoxia and hypercapnia, the arousal responses to asphyxial apnea, and the acclimatization to high altitude. In response to an excitatory stimuli, glomus cells in the carotid body depolarize, their intracellular calc...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.10190

    authors: Gauda EB

    更新日期:2002-11-01 00:00:00

  • Germinal vesicle chromatin configurations of bovine oocytes.

    abstract::A common feature in the configuration of germinal vesicle (GV) chromatin in most species is that diffuse chromatin condenses into a perinucleolar ring during follicular growth; however, no such ring was observed in goat oocytes. Reports on whether bovine GV chromatin condenses into a perinucleolar ring are controversi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20349

    authors: Liu Y,Sui HS,Wang HL,Yuan JH,Luo MJ,Xia P,Tan JH

    更新日期:2006-10-01 00:00:00

  • A descriptive study to provide evidence of the teratogenic and cellular effects of sibutramine and ephedrine on cardiac- and liver-tissue of chick embryos.

    abstract::Exposure to drugs during pregnancy is a major concern, as some teratogenic compounds can influence normal foetal development. Although the use of drugs during pregnancy should generally be avoided, exposure of the developing foetus to teratogens may occur unknowingly since these compounds may be hidden in products tha...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22539

    authors: Oberholzer HM,Van Der Schoor C,Taute H,Bester MJ

    更新日期:2015-08-01 00:00:00

  • Ionic composition of rat airway surface liquid determined by X-ray microanalysis.

    abstract::The thin layer of liquid that lines the conducting airway epithelium, the airway surface liquid (ASL), is important for mucociliary clearance. Altered ionic composition and/ or volume of the ASL play a major role in the pathology of airway diseases such as cystic fibrosis. Since the ASL is a thin layer, it has been di...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20217

    authors: Vanthanouvong V,Kozlova I,Roomans GM

    更新日期:2005-09-01 00:00:00

  • Ultrastructural study of apoptotic U937 [corrected] cells treated with different pro-apoptotic substances.

    abstract::Human monocytic leukemia U937 cells readily undergo apoptosis when exposed to various stimuli, including inhibition of protein synthesis, oxidative stress, antitumoral agents, etc. The sequential, step-by-step morphological changes in U937 cells that occur during the apoptotic program are largely determined by the act...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20058

    authors: Abbro L,Lanubile R,Dini L

    更新日期:2004-05-01 00:00:00

  • Describing one generic and two new species record to the flora of Pakistan using LM and SEM methods.

    abstract::Common mulberry weed (Fatoua villosa (Thunb.) Nakai) and creeping amaranth (Amaranthus crassipes Schlecht) are reported for the first time in Pakistan's flora as these were not listed in any other literature nor identified before in Pakistan. Plants were found as a result of taxonomic studies performed in 2013 in Pesh...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23000

    authors: Jamshed S,Ullah A,Khan R,Hussain A

    更新日期:2018-05-01 00:00:00

  • Scanning electron microscopy imaging of dislocations in bulk materials, using electron channeling contrast.

    abstract::The imaging and characterization of dislocations is commonly carried out by thin foil transmission electron microscopy (TEM) using diffraction contrast imaging. However, the thin foil approach is limited by difficult sample preparation, thin foil artifacts, relatively small viewable areas, and constraints on carrying ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.20293

    authors: Crimp MA

    更新日期:2006-05-01 00:00:00

  • Determination of conditions for optimum resolution of a high angle thin window energy dispersive spectrometer.

    abstract::The energy resolution of an energy dispersive spectrometer (EDS) equipped with an ultrathin window (UTW) and mounted at a high take-off angle (72 degrees) on a transmission electron microscope has been studied under a variety of operating conditions. The spectrometer resolution is close to that specified by the manufa...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070220208

    authors: Blackford MG,Smith KL

    更新日期:1992-07-01 00:00:00

  • Spatial distribution of cytoskeleton intermediate filaments during fetal rat hepatocyte differentiation.

    abstract::The construction of the liver parenchyma throughout fetal development depends on the elaboration of intercellular contacts between epithelial cells and between epithelial and mesenchymal cells. During this time, the spatial distribution of cytokeratins in hepatocytes shows a striking evolution as demonstrated by confo...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19971201)39:5<436::AID-JEM

    authors: Vassy J,Irinopoulou T,Beil M,Rigaut JP

    更新日期:1997-12-01 00:00:00

  • Visualizing subsurface defects in graphite by acoustic atomic force microscopy.

    abstract::We describe a versatile platform, which combines atomic force acoustic microscopy, ultrasonic atomic force microscopy and heterodyne force microscopy. The AFM system can enable in-situ switching among these operation modes flexibly and thus benefit the discrimination of differences in mechanical properties and buried ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22668

    authors: Wang T,Ma C,Hu W,Chen Y,Chu J

    更新日期:2017-01-01 00:00:00