Abstract:
:We present a new beam-shaping technique for two-color excitation fluorescence microscopy. We show that by simply inserting a properly designed shaded-ring filter in the illumination beam of smaller wavelength, it is possible to improve the effective optical sectioning capacity of such microscopes by 23%. Such an improvement is obtained at the expense of only a very small increasing of the overall energy in the point-spread-function sidelobes. The performance of this technique is illustrated by a numerical imaging simulation.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Ibáñez-López C,Escobar I,Saavedra G,Martínez-Corral Mdoi
10.1002/jemt.20073keywords:
subject
Has Abstractpub_date
2004-06-01 00:00:00pages
96-102issue
2eissn
1059-910Xissn
1097-0029journal_volume
64pub_type
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