Optical-sectioning improvement in two-color excitation scanning microscopy.

Abstract:

:We present a new beam-shaping technique for two-color excitation fluorescence microscopy. We show that by simply inserting a properly designed shaded-ring filter in the illumination beam of smaller wavelength, it is possible to improve the effective optical sectioning capacity of such microscopes by 23%. Such an improvement is obtained at the expense of only a very small increasing of the overall energy in the point-spread-function sidelobes. The performance of this technique is illustrated by a numerical imaging simulation.

journal_name

Microsc Res Tech

authors

Ibáñez-López C,Escobar I,Saavedra G,Martínez-Corral M

doi

10.1002/jemt.20073

keywords:

subject

Has Abstract

pub_date

2004-06-01 00:00:00

pages

96-102

issue

2

eissn

1059-910X

issn

1097-0029

journal_volume

64

pub_type

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