Abstract:
:Haematoxylin/Eosin staining was performed to screen for Hirano bodies from the temporal lobe including the hippocampus in 10 Pick's disease cases containing Pick bodies. Although the inclusions were confirmed in 9 out of 10 cases, only 4 out of 10 were particularly enriched with the eosinophilic bodies. These were subjected to immunostaining with anticomplement antibodies and astrocyte marker antiglial fibrillary acidic protein antibody and the HLA class II CR3/43 antibody to visualize microglia. An intraneuronal Hirano body was observed in one case that otherwise contained mainly the extracellular inclusions. In all cases, the extracellular Hirano bodies were seen lying adjacent to soma of neurons within CA1 region of the hippocampus. The extracellular Hirano bodies stained intensely with C1q, the first component of the classical pathway of activation but remained unreactive against antibodies to C4 and the C3 activation products (C3b and iC3b) and the alternative complement pathway component factor B. Hirano bodies also remained negative with the antiglial fibrillary acidic protein for astrocytes and HLA class II antibody CR3/43 for microglia. The results demonstrate that Hirano bodies have strong immunoreactivity to C1q; however, whether other complement components are associated with these inclusions remains to be further investigated.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Singhrao SKdoi
10.1002/jemt.22206subject
Has Abstractpub_date
2013-06-01 00:00:00pages
606-11issue
6eissn
1059-910Xissn
1097-0029journal_volume
76pub_type
杂志文章abstract::This study was designed to investigate patterns of fibrils organization in histochemically stained otoconia. Transmission electron microscope and video imaging were used. These data indicate that otoconia of the chick (Gallus domesticus) inner ear may have central cores in vivo. The data also show that the ultrastruct...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070250406
更新日期:1993-07-01 00:00:00
abstract::This work provides examples of some of the imaging capabilities of environmental scanning electron microscopy applied to easily charged samples relevant to particle analysis. Environmental SEM (also referred to as high pressure or low vacuum SEM) can address uncoated samples that are known to be difficult to image. Mo...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070250507
更新日期:1993-08-01 00:00:00
abstract::Using high resolution transmission electron microscopy with image simulation and Fourier analysis, the Li1- x FePO4 (x < 0.01), Li1- x FePO4 (x ∼ 0.5), and FePO4 particles, prepared by charging or discharging the 053048 electrochemical cells (thickness: 5 mm, width: 30 mm, height: 48 mm) and dismantled inside an Ar-fi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22495
更新日期:2015-06-01 00:00:00
abstract::Breast cancer is the most common cancer diagnosed among women. In this article, support vector machine is used to classify digital mammogram images into malignant and benign. Wiener filter is used to handle the possible quantum noise, which is more likely to occur in mammograms. Stack-based connected component method ...
journal_title:Microscopy research and technique
pub_type: 信件
doi:10.1002/jemt.21070
更新日期:2011-11-01 00:00:00
abstract::The present paper covers the results of different transmission electron microscopy studies on silver halide microcrystals. Pure AgBr as well as core-shell AgBr-AgBrI crystals are investigated. In the former parallel and non-parallel twinning modes yielding tabular and needle- or tetrahedral-shaped microcrystals, respe...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980715)42:2<85::AID-JEMT
更新日期:1998-07-15 00:00:00
abstract::High speed data registration is required for the study of fluorescence resonance energy transfer in real time as well as fast dynamic intra- and inter-cellular signaling events. Multispectral confocal spinning disk microscopy provides a high resolution method for performing such real time live cell imaging. However, o...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20423
更新日期:2007-04-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19981015)43:2<131::AID-JEM
更新日期:1998-10-15 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070280302
更新日期:1994-06-15 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980301)40:6<488::AID-JEM
更新日期:1998-03-15 00:00:00
abstract::Photoconversion of fluorescent staining into stable diaminobenzidine (DAB) precipitate is widely used for neuroanatomical and developmental studies. An important advantage of the approach is to make correlations between light and electron microscopy analyses possible, the DAB reaction product formed during photoconver...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20239
更新日期:2005-10-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22659
更新日期:2016-06-01 00:00:00
abstract::Effective protocols for controlling crystal structure, size, and morphology attract considerable interest given the requirement for particles of modal size and shape in many areas of materials fabrication and the importance of crystallochemical selectivity in determining the exploitable properties of inorganic solids....
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070270504
更新日期:1994-04-01 00:00:00
abstract::Growth-year authentication has extraordinary significance for plant growth, structure and development research, and has a wide range of applications in value assessment of economic crops. Panax ginseng is the most commonly used medicinal plant in Asian countries. The fix number of growth-year is an important quality e...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22610
更新日期:2016-02-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10080
更新日期:2002-05-15 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22255
更新日期:2013-09-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20107
更新日期:2004-09-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070230104
更新日期:1992-10-01 00:00:00
abstract::Neurons begin to polarize when one of the neurites becomes the axon. Hippocampal neurons in cell culture have a sharp transition between their unpolarized and polarized stage revealed by the rapid growth of the future axon. Recent progress shows that both a cytoplasmic membrane flow and actin dynamics govern axon form...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(20000101)48:1<3::AID-JEMT2
更新日期:2000-01-01 00:00:00
abstract::Apanteles cypris is an endoparasitoid of rice pest Cnaphalocrocis medinalis. To better understand its host location mechanism, we examined the external morphology of antennal sensilla of A. cypris via scanning electron microscopy. The antennae of male and female A. cypris were filiform in shape, which consist of scape...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.21016
更新日期:2011-12-01 00:00:00
abstract::This bibliography is compiled to assist in locating papers related to the application of scanning electron microscopy (SEM) to cerebrospinal-fluid-contacting surfaces in vertebrates. The use of SEM by neuroscientists has continued apace since the publication of the first bibliography in 1980. SEM studies now include m...
journal_title:Microscopy research and technique
pub_type:
doi:10.1002/(SICI)1097-0029(19980415)41:2<158::AID-JEM
更新日期:1998-04-15 00:00:00
abstract::This study compared several irrigation protocols and application systems for sealer penetration into dentinal tubules. Single-rooted-human teeth were divided into 5 experimental groups (n = 15) and a control group (n = 5), according to final irrigation protocols: standard needle irrigation (SNI); Vibringe; Vibringe + ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22944
更新日期:2017-12-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22024
更新日期:2012-07-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23118
更新日期:2018-10-01 00:00:00
abstract::The ultrastructure of adult human articular cartilage matrix is reexamined in tissue processed according to recently improved cryotechniques [Studer et al. (1995) J. Microsc., 179:321-332]. In truely vitrified tissue, a network of fine cross-banded filaments (10-15 nm in diameter) with a periodicity characteristic of ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970515)37:4<271::AID-JEM
更新日期:1997-05-15 00:00:00
abstract::The anatomical substrate for coordinated release from the dispersed gonadotropin-releasing hormone (GnRH) neuronal population remains obscure. There is physiological evidence that the GnRH hormone itself has a role in tonic inhibition or modulation of GnRH function. This has led to the hypothesis that there is an ultr...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19990101)44:1<11::AID-JEMT
更新日期:1999-01-01 00:00:00
abstract::In this article, the Ni-Cu nanoparticles (NPs) in the amorphous carbon hydrogenated thin films with different copper percentage by co-deposition of RF-plasma enhanced chemical vapor deposition were prepared using acetylene gas and Ni and Cu targets. The films deposited with 5% Cu have minimum value of the average diam...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23679
更新日期:2020-12-22 00:00:00
abstract::We discuss the use of fluorescence correlation spectroscopy for the measurement of relatively slow rotations of large macromolecules in solution or attached to other macromolecular structures. We present simulations and experimental results to illustrate the range of rotational correlation times and diffusion times th...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20121
更新日期:2004-11-01 00:00:00
abstract::In situ hybridization (ISH) for HIV is an arduous, demanding means of detecting viral genetic material in cells and tissues. Good ISH requires broad technical skills and devotion to controls for every step of the process as well as a critical eye when interpreting results. ISH may be used to detect HIV in three ways: ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070250111
更新日期:1993-05-01 00:00:00
abstract::The skin is a tissue containing a large number of collagen types. Several collagens are restricted at the dermo-epidermal junction, contrarily to others present throughout the dermis. However, the distribution of the dermal collagen varies during embryonic development. In this contribution, we have been interested in ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970815)38:4<407::AID-JEM
更新日期:1997-08-15 00:00:00
abstract::The field of quantitative analysis and subsequent mapping of the cerebral cortex has developed rapidly. New powerful tools have been applied to investigate large regions of complex folded gyrencephalic cortices in order to detect structural transition regions that might partition different cortical fields of disjunct ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20033
更新日期:2004-03-01 00:00:00