Abstract:
:Low voltage electron microscopes working in transmission mode, like LVEM5 (Delong Instruments, Czech Republic) working at accelerating voltage 5 kV or scanning electron microscope working in transmission mode with accelerating voltage below 1 kV, require ultrathin sections with the thickness below 20 nm. Decreasing of the primary electron energy leads to enhancement of image contrast, which is especially useful in the case of biological samples composed of elements with low atomic numbers. As a result treatments with heavy metals, like post-fixation with osmium tetroxide or ultrathin section staining, can by omitted. The disadvantage is reduced penetration ability of incident electrons influencing the usable thickness of the specimen resulting in the need of ultrathin sections of under 20 nm thickness. In this study we want to answer basic questions concerning the cutting of extremely ultrathin sections: Is it possible routinely and reproducibly to cut extremely thin sections of biological specimens embedded in commonly used resins with contemporary ultramicrotome techniques and under what conditions? Microsc. Res. Tech. 79:512-517, 2016. © 2016 Wiley Periodicals, Inc.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Nebesářová J,Hozák P,Frank L,Štěpan P,Vancová Mdoi
10.1002/jemt.22659subject
Has Abstractpub_date
2016-06-01 00:00:00pages
512-7issue
6eissn
1059-910Xissn
1097-0029journal_volume
79pub_type
杂志文章abstract::We combine reflective confocal microscopy with multiphoton microscopy to form a minimally invasive technique to observe the cornea. The two imaging modalities allow detection of complementary information from the cornea. The autofluorescence signal shows the cytoplasm of epithelial cells, and the second harmonic gener...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20528
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abstract::The Dominican Republic (DR) has experienced tremendous increase in the number of cases of dengue virus (DENV) reported in the past few years. There are four serotypes of DENV (1-4), and each can cause classic dengue fever (DF), dengue hemorrhagic fever (DHF), or dengue shock syndrome (DSS). DENV-1-4 are currently circ...
journal_title:Microscopy research and technique
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abstract::High speed data registration is required for the study of fluorescence resonance energy transfer in real time as well as fast dynamic intra- and inter-cellular signaling events. Multispectral confocal spinning disk microscopy provides a high resolution method for performing such real time live cell imaging. However, o...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20423
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abstract::This review article focuses on the ontogeny and the regulatory mechanisms involved in the modulation of the intracellular events governing the assembly and delivery of lipoproteins in human gut. The human fetal intestine organizes villi covered with well-differentiated enterocytes during the end of the first trimester...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(20000515)49:4<363::AID-JEM
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abstract::Microscopic magnetic resonance imaging (µMRI) T2 data from canine cartilage at different tibial locations were analyzed to investigate the influences of spatial resolution and pixel position on the T2 sensitivity to osteoarthritis (OA). Five experimental factors were investigated: inaccurate pixel position, different ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22694
更新日期:2016-08-01 00:00:00
abstract::The mint beetle or green mint beetle, Chrysolina herbacea, is one of the most important insect pests of mint herb. These insects are associated with plants belonging to the Lamiaceae family, in particular the Mentha genus. They cause serious damages to plants by consuming their leaves. For this reason, it is an import...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23671
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abstract::This work provides examples of some of the imaging capabilities of environmental scanning electron microscopy applied to easily charged samples relevant to particle analysis. Environmental SEM (also referred to as high pressure or low vacuum SEM) can address uncoated samples that are known to be difficult to image. Mo...
journal_title:Microscopy research and technique
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abstract::Basement membranes (BMs) were first described in the mid-19th century, but they were not isolated and prepared for compositional studies until nearly 100 years later. Early methods of isolation were carried out on renal glomeruli, which were first sub-fractionated from kidney tissues by sieving. BMs were then isolated...
journal_title:Microscopy research and technique
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abstract::Transit from the nuclear complex to the cytoplasm through the nuclear pore complex permits modification of mRNA, including processing such as splicing, capping, and polyadenylation, etc. At each of these events, mRNA interacts with various proteins to form mRNA-protein complex. Visualizing the mRNA is crucial for unde...
journal_title:Microscopy research and technique
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doi:10.1002/jemt.23214
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abstract::The apolipoprotein E (apoE) epsilon4 allele increases risk of Alzheimer's disease (AD), perhaps by accelerating plaque formation, or by impairing neuron repair. Considerable evidence supports both mechanisms. AD patients with epsilon4 have more and earlier amyloid deposits than do patients without epsilon4. The same i...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20000815)50:4<278::AID-JEMT5>3.0
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abstract::When 20-day-old rats are placed on a vitamin A deficient diet (VAD) for a period of 10 weeks, the seminiferous tubules are found to contain only Sertoli cells, a few residual A0, A1 spermatogonia, and preleptotene spermatocytes (PL). The type A1 spermatogonia and PL spermatocytes are arrested in their G2 phase. In VAD...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070200106
更新日期:1992-01-01 00:00:00
abstract::Lignocellulosic plant cell wall is considered a potential source for second generation biofuels. The plant cell wall is a highly complex structure mainly composed of cellulose, hemicelluloses, and lignin that form a network of crosslinked fibers. The structural organization of the sugarcane cell wall has not been prev...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22235
更新日期:2013-08-01 00:00:00
abstract::The implantation of breast prostheses for both aesthetic and reconstructive purposes has been growing exponentially in the last 20 years. Safety and prosthesis lifespan are majorly debated issues in relation to the correlated long-term complications. Mainly the periprosthetic capsule that develops around the implant i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23673
更新日期:2020-12-10 00:00:00
abstract::Worldwide, breast cancer (BrCa) is currently the leading cause of deaths associated to malignant lesions in adult women. Given that some studies have mentioned that peritumoral adipocytes may contribute to breast carcinogenesis, present work sought to quantitative evaluate the morphometry of these cells in a group of ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22972
更新日期:2018-02-01 00:00:00
abstract::The cyclic fluctuations in circulating levels of 17 beta-estradiol and progesterone that occur during the menstrual or estrous cycle are responsible for dramatic, cyclic changes in the epithelial lining and secretory status of the mammalian oviduct. The timely transition in the synthesis and release of oviduct protein...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070310606
更新日期:1995-08-15 00:00:00
abstract::Substance P (SP) after binding to the neurokinin-1 (NK-1) receptor regulates many biological functions. Both SP and the NK-1 receptor are expressed in human normal placenta cells, monocytes, and macrophages. However, to our knowledge, the presence of both SP and the NK-1 receptor in macrophages of the placenta, the Ho...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22303
更新日期:2013-12-01 00:00:00
abstract::To study the effect of microwaves on immunolabeling, ultrathin cryosections or diluted antibodies were irradiated prior to antibody application, and gold labeling was quantified. In addition, affinity purified, polyclonal antibodies and protein A-gold were applied to ultrathin cryosections of aldehyde-fixed material i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980701)42:1<24::AID-JEMT
更新日期:1998-07-01 00:00:00
abstract::Puffers show good drag reduction performance during migration. It is worth noting that spines which are different from ordinary fish scales are densely distributed on the puffer skin. Here, the special morphological structure of puffer spines was observed using microscopy techniques, accurate contour models were estab...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23470
更新日期:2020-07-01 00:00:00
abstract::This study was conducted to provide comprehensive information on the anatomical and histological features of the upper (UL) and lower (LL) lips of Rahmani sheep by gross examination, morphometric analysis in addition to Light and scanning electron microscope (SEM). Samples from normal healthy adult male sheep heads we...
journal_title:Microscopy research and technique
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doi:10.1002/jemt.23660
更新日期:2020-12-01 00:00:00
abstract::The mammalian gallbladder mucosa is lined by a simple columnar epithelium. Typical surface epithelial cells (principal cells) contain short microvilli, secretory granules, dense bodies, mitochondria and Golgi apparatus. Dense bodies are thought to be lysosomes. Secretory granules contain mucous glycoproteins which are...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970915)38:6<616::AID-JEM
更新日期:1997-09-15 00:00:00
abstract::Transfer of a fluorescently labeled protein particle from a surface to a microsized scanning probe has been induced by repetitive scanning in aqueous medium. The so-attached particle can in turn act as a probing tool to study particle-substrate and particle-particle interactions. Attachment of the fluorescent particle...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20816
更新日期:2010-08-01 00:00:00
abstract::This study evaluated the fluoride (F) release and remineralizing potential of varnishes containing sodium fluoride (5% NaF), 5% NaF with CPP-ACP and 5% NaF with TCP in early caries lesions in primary teeth. To determine the F release at 1, 4, 6, 24, 72, and 168 hr, strips were covered with the varnishes and immersed i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23662
更新日期:2020-12-05 00:00:00
abstract::The fine structure of human oogonia and growing oocytes has been reviewed in fetal and adult ovaries. Preovulatory maturation and the ultrastructure of stimulated oocytes from the germinal vesicle (GV) stage to metaphase II (MII) stage are also documented. Oogonia have large nuclei, scanty cytoplasm with complex mitoc...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20299
更新日期:2006-06-01 00:00:00
abstract::Sensory mechanisms in teeth are not well understood and may involve pulpal-neural interactions. Tooth cells that proliferate in vitro have polyclonal immunoreactivity (IR) for glial fibrillary acidic protein (GFAP), growth-associated protein (GAP-43), and vimentin, plus glial-like ion channels. Here, we analyzed GFAP-...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20130
更新日期:2004-12-01 00:00:00
abstract::Surface ultrastructures of gill filaments and secondary lamellae of Rita rita and Cirrhinus mrigala, inhabiting different ecological habitat, were investigated to unravel adaptive modifications. R. rita is a sluggish, bottom dwelling carnivorous catfish, which inhabits regions of river with accumulations of dirty wate...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.21074
更新日期:2012-04-01 00:00:00
abstract::Fipronil, active ingredient of Frontline® acaricide, is a phenyl-pyrazolic derivative, and its efficacy in the elimination of several plagues, even in lower concentrations, has already been demonstrated; however, its effect on nontarget organisms has not been thoroughly explained. In this sense, the present study had ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.21053
更新日期:2012-03-01 00:00:00
abstract::Cartilage morphogenesis is a prerequisite for skeletal development and maintenance. The morphogenesis of cartilage determines the shape of bones, and joints including articular cartilage, ligaments, and tendon. This article reviews the recent advances in cartilage-derived morphogenetic proteins (CDMPs) and related bon...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19981015)43:2<131::AID-JEM
更新日期:1998-10-15 00:00:00
abstract::Uranium-contaminated soils from the U.S. Department of Energy (DOE) Fernald Site, Ohio, have been examined by a combination of backscattered electron imaging (BSE) and analytical electron microscopy with electron diffraction (AEM). The inhomogeneous distribution of particulate uranium phases in the soil required the d...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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更新日期:1995-06-01 00:00:00
abstract::Malaria is a serious worldwide disease, caused by a bite of a female Anopheles mosquito. The parasite transferred into complex life round in which it is grown and reproduces into the human body. The detection and recognition of Plasmodium species are possible and efficient through a process called staining (Giemsa). T...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23269
更新日期:2019-07-01 00:00:00
abstract::The rapid photothermal lysis of Escherichia coli O157:H7 treated with light-triggered Janus nanomotors was visualized by Hilbert differential contrast transmission electron microscopy (HDC-TEM). The extraordinary advantage of this high-resolution microscopic technique was that it revealed the detailed ultrastructure a...
journal_title:Microscopy research and technique
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更新日期:2020-11-27 00:00:00