Abstract:
:Broiler chicken and laying hen livers were fixed using a simple technique of in situ puncture perfusion of cacodylate-buffered fixative, which allowed characterisation of the fine structure of hepatic parenchyma, hepatocytes, bile ductules, and, in particular, the sinusoidal cells including endothelial, Kupffer, and Ito cells. Sinusoidal endothelial cells with their bulging perinuclear cytoplasm, evident in both transmission and scanning electron micrographs, were easily distinguishable from Kupffer cells, which possessed numerous pseudopodia. Bile ductular epithelium and hepatocytes of the laying hens contained large amounts of lipid. The ultrastructural characteristics of intercalated cells (putative extra-sinusoidal macrophages of chicken liver) are described and their possible role as precursors of Kupffer cells is discussed.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Ghoddusi M,Kelly WRdoi
10.1002/jemt.20107keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
101-11issue
1-2eissn
1059-910Xissn
1097-0029journal_volume
65pub_type
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