Ultrastructure of in situ perfusion-fixed avian liver, with special reference to structure of the sinusoids.

Abstract:

:Broiler chicken and laying hen livers were fixed using a simple technique of in situ puncture perfusion of cacodylate-buffered fixative, which allowed characterisation of the fine structure of hepatic parenchyma, hepatocytes, bile ductules, and, in particular, the sinusoidal cells including endothelial, Kupffer, and Ito cells. Sinusoidal endothelial cells with their bulging perinuclear cytoplasm, evident in both transmission and scanning electron micrographs, were easily distinguishable from Kupffer cells, which possessed numerous pseudopodia. Bile ductular epithelium and hepatocytes of the laying hens contained large amounts of lipid. The ultrastructural characteristics of intercalated cells (putative extra-sinusoidal macrophages of chicken liver) are described and their possible role as precursors of Kupffer cells is discussed.

journal_name

Microsc Res Tech

authors

Ghoddusi M,Kelly WR

doi

10.1002/jemt.20107

keywords:

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

101-11

issue

1-2

eissn

1059-910X

issn

1097-0029

journal_volume

65

pub_type

杂志文章
  • Immuno-detection of mRNA-binding protein complex in human cells under transmission electron microscopy.

    abstract::Transit from the nuclear complex to the cytoplasm through the nuclear pore complex permits modification of mRNA, including processing such as splicing, capping, and polyadenylation, etc. At each of these events, mRNA interacts with various proteins to form mRNA-protein complex. Visualizing the mRNA is crucial for unde...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23214

    authors: Ma Q,Tatsuno T,Nakamura Y,Izumi SI,Tomosugi N,Ishigaki Y

    更新日期:2019-06-01 00:00:00

  • Taxonomic importance of pollen morphology in Veratrum L. (Melanthiaceae) using microscopic techniques.

    abstract::Previous studies reported that intrageneric relationships of genus Veratrum of family Melanthiaceae are controversial and hard to delimit. Therefore, we observed the pollen morphological features of six species in the genus Veratrum in detail using both light microscopy and scanning electron microscopy and investigate...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23479

    authors: Hu Z,Zhao Y,Zhao C,Liu J

    更新日期:2020-08-01 00:00:00

  • Distribution of the catecholaminergic neurons in the central nervous system of human embryos and fetuses.

    abstract::The catecholaminergic cell groups in the human brain, denominated from A1 to A17, display some striking anatomical differences with those described in the rodent. These differences are essentially observed in the extent of the dopaminergic neurons and especially their axonal fields in the telencephalon. Immunocytochem...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19990701)46:1<24::AID-JEMT

    authors: Verney C

    更新日期:1999-07-01 00:00:00

  • Imaging of three-dimensional epithelial architecture and function in cultured CaCo2a monolayers with two-photon excitation microscopy.

    abstract::The principal functions of the gastrointestinal tract mucosa include nutrient absorption, protein and fluid secretion, and the regulated symbiosis with intraluminal contents. Research in epithelial biology has benefited significantly from the use of cultured monolayer preparations, which closely replicate the structur...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/1097-0029(20001015)51:2<204::AID-JEMT11>3.

    authors: Gilbert RJ,Hoffman M,Capitano A,So PT

    更新日期:2000-10-15 00:00:00

  • Relationships between the central spindle and the contractile ring during cytokinesis in animal cells.

    abstract::During late anaphase and telophase, animal cells develop a bundle of antiparallel, interdigitating microtubules between the two daughter nuclei. Recent data indicate that this structure, called the central spindle, plays an essential role during cytokinesis. Studies in Drosophila and on vertebrate cells strongly sugge...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(20000415)49:2<202::AID-JEM

    authors: Gatti M,Giansanti MG,Bonaccorsi S

    更新日期:2000-04-15 00:00:00

  • Developmental aspects of lipid and lipoprotein synthesis and secretion in human gut.

    abstract::This review article focuses on the ontogeny and the regulatory mechanisms involved in the modulation of the intracellular events governing the assembly and delivery of lipoproteins in human gut. The human fetal intestine organizes villi covered with well-differentiated enterocytes during the end of the first trimester...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(20000515)49:4<363::AID-JEM

    authors: Levy E,Ménard D

    更新日期:2000-05-15 00:00:00

  • Apolipoprotein E isoforms in Alzheimer's disease pathology and etiology.

    abstract::The apolipoprotein E (apoE) epsilon4 allele increases risk of Alzheimer's disease (AD), perhaps by accelerating plaque formation, or by impairing neuron repair. Considerable evidence supports both mechanisms. AD patients with epsilon4 have more and earlier amyloid deposits than do patients without epsilon4. The same i...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/1097-0029(20000815)50:4<278::AID-JEMT5>3.0

    authors: Baum L,Chen L,Ng HK,Pang CP

    更新日期:2000-08-15 00:00:00

  • Nitric oxide modulates sympathetic neurotransmission at the prejunctional level.

    abstract::In spite of accumulating evidence for a modulation of sympathetic neurotransmission by endogenously produced nitric oxide (NO), it remains unclear in which parts of the vascular system and at what level this interaction takes place. The aim of the present study was to investigate the distribution of endothelial and ne...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070290214

    authors: Addicks K,Bloch W,Feelisch M

    更新日期:1994-10-01 00:00:00

  • Intrinsic physicochemical interactions of calcium hydroxide-based medications.

    abstract::To investigate intrinsic physicochemical properties and interactions of three different calcium hydroxide-based medications via means of different analytical methods. Two-commercial premixed medications: TempCanal(TCmx) and ProCalR(PCmx) and powder-form ProCal(PCpw) with glycerin were used. Vibrational modes were anal...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23600

    authors: Uslu O,Dikmen G,Orhan EO

    更新日期:2020-09-21 00:00:00

  • Three-dimensional structure of the zona pellucida at ovulation.

    abstract::The mammalian zona pellucida (ZP) is an extracellular matrix surrounding oocytes and early embryos, which is critical for normal fertilization and preimplantation development. It is made up of three/four glycoproteins arranged in a delicate filamentous matrix. Scanning electron microscopy (SEM) studies have shown that...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.20301

    authors: Familiari G,Relucenti M,Heyn R,Micara G,Correr S

    更新日期:2006-06-01 00:00:00

  • E-cadherin and catenins: molecules with versatile roles in normal and neoplastic epithelial cell biology.

    abstract::E-cadherin and its associated cytoplasmic proteins alpha-, beta-, and gamma-catenin, play a crucial role in epithelial cell-cell adhesion and in the maintenance of tissue architecture. Perturbation in the expression or function of any of these molecules results in loss of intercellular adhesion, with possible conseque...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19981101)43:3<224::AID-JEM

    authors: El-Bahrawy MA,Pignatelli M

    更新日期:1998-11-01 00:00:00

  • Immune response to autologous and heterologous Helicobacter pylori antigens in humans.

    abstract::Infection of humans with Helicobacter pylori results in the development of chronic gastritis and plays an important role in gastric ulcer pathogenesis. Despite the infiltration of the mucosa with specific immunocompetent cells and production of specific antibodies, the infection usually persists for life. This study w...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1111

    authors: Jakob B,Birkholz S,Schneider T,Duchmann R,Zeitz M,Stallmach A

    更新日期:2001-06-15 00:00:00

  • Structure and function in the ruminant synepitheliochorial placenta: central role of the trophoblast binucleate cell in deer.

    abstract::The ruminant placenta has a very uniform gross structure based on localised areas of fetomaternal membrane apposition and proliferation to form placentomes. There is no consistency, however, in the number or size of these placentomes (6-150), nor in the villus architecture of the individual placentome. The one consist...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19970701/15)38:1/2<88::AID

    authors: Wooding FB,Morgan G,Adam CL

    更新日期:1997-07-01 00:00:00

  • Micromorphology, pharmacognosy, and bio-elemental analysis of an important medicinal herb: Verbascum thapsus L.

    abstract::The present study was conducted on characterization of morpho-anatomical, phytochemical, and bio-elemental analysis of root, stem, and leaf of Verbascum thapsus. Morphologically Verbascum is a biennial plant that flowers for a month and a half in mid- to late summer. Various organoleptic features of root, leaf, and st...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23454

    authors: Khan SA,Dastagir G,Uza NU,Muhammad A,Ullah R

    更新日期:2020-06-01 00:00:00

  • Image processing techniques in a preliminary morphometric characterization of corneal epithelium by scanning electron microscopy.

    abstract::Several processing techniques of digital images allowed us to quantify the percentage of cell surface covered by microprojections (microvilli or microplicae) (SCM), the adhesion between epithelial cells by the parameter intercellular junctions (IJ), the size (cell area), shape (cell shape) and shade (cell shade) of ce...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20833

    authors: Julio G,Merindano D,Canals M,Ralló M

    更新日期:2010-10-01 00:00:00

  • Imaging molecular structure and physiological function of gap junctions and hemijunctions by multimodal atomic force microscopy.

    abstract::Gap junctions are specialized plasma membrane structures that join neighboring cells via specialized intercellular ion channels (hemichannels) and provide a direct pathway for cell-cell communication. They presumably mediate regulation of growth, transmission of developmental signals, coordination of muscle contractio...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/1097-0029(20010201)52:3<273::AID-JEMT1013>

    authors: Lal R,Lin H

    更新日期:2001-02-01 00:00:00

  • Inhibition of H2O2-induced apoptosis of lymphocytes by calorie restriction during aging.

    abstract::Calorie restriction (CR) is known to delay the aging process in rodents and is postulated to act by decreasing free radical generation and increasing antioxidant enzyme activity. The present study was designed to investigate the effect of CR and age on oxidative stress-induced apoptosis and associated changes in the l...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.10206

    authors: Avula CP,Fernandes G

    更新日期:2002-11-15 00:00:00

  • From oogonia to mature oocytes: inactivation of the maternal centrosome in humans.

    abstract::The fine structure of human oogonia and growing oocytes has been reviewed in fetal and adult ovaries. Preovulatory maturation and the ultrastructure of stimulated oocytes from the germinal vesicle (GV) stage to metaphase II (MII) stage are also documented. Oogonia have large nuclei, scanty cytoplasm with complex mitoc...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.20299

    authors: Sathananthan AH,Selvaraj K,Girijashankar ML,Ganesh V,Selvaraj P,Trounson AO

    更新日期:2006-06-01 00:00:00

  • Changes in membrane trafficking and actin dynamics during axon formation in cultured hippocampal neurons.

    abstract::Neurons begin to polarize when one of the neurites becomes the axon. Hippocampal neurons in cell culture have a sharp transition between their unpolarized and polarized stage revealed by the rapid growth of the future axon. Recent progress shows that both a cytoplasmic membrane flow and actin dynamics govern axon form...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(20000101)48:1<3::AID-JEMT2

    authors: Bradke F,Dotti CG

    更新日期:2000-01-01 00:00:00

  • Cartilage-derived morphogenetic proteins and cartilage morphogenesis.

    abstract::Cartilage morphogenesis is a prerequisite for skeletal development and maintenance. The morphogenesis of cartilage determines the shape of bones, and joints including articular cartilage, ligaments, and tendon. This article reviews the recent advances in cartilage-derived morphogenetic proteins (CDMPs) and related bon...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19981015)43:2<131::AID-JEM

    authors: Reddi AH

    更新日期:1998-10-15 00:00:00

  • Apatite-like forming ability, porosity, and bond strength of calcium aluminate cement with chitosan, zirconium oxide, and hydroxyapatite additives.

    abstract::This study evaluated the effect of chitosan, zirconium oxide, and hydroxyapatite on the apatite-like forming ability, porosity, and bond-strength of calcium-aluminate cements (C). Three hundred bovine root-slices were assigned to one of five groups, according to the material: MTA, C, C + chitosan (Cchi), C + zirconium...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23678

    authors: Saltareli FM,Leoni GB,de Lima Ferraz Aguiar N,de Faria NS,Oliveira IR,Bachmann L,Raucci-Neto W

    更新日期:2020-12-22 00:00:00

  • Characterization of elemental distribution across human dentin-enamel junction by scanning electron microscopy with energy-dispersive X-ray spectroscopy.

    abstract::The human dentin-enamel junction (DEJ) is a natural junction that unites two dissimilar mineralized tissues in the human tooth: enamel and dentin. DEJ plays a critical role in maintaining structural and functional integrity of the tooth. However, its structure, chemical composition and function remain unclear and cont...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23648

    authors: Wang R,Zhao D,Wang Y

    更新日期:2020-11-19 00:00:00

  • Development of pigment cells in the zebrafish embryo.

    abstract::In recent years, the zebrafish Danio rerio has emerged as a powerful model organism for the study of vertebrate embryogenesis. Zebrafish, like other vertebrates, possess pigment cells that arise from two distinct embryonic sources: those of the dermis and epidermis originate from the neural crest, while those that com...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.10161

    authors: Lister JA

    更新日期:2002-09-15 00:00:00

  • Identification of ages and determination of paeoniflorin in roots of Paeonia lactiflora Pall. From four producing areas based on growth rings.

    abstract::Growth rings were used to determine the root age of medicinal Paeonia lactiflora from four producing areas, and their corresponding paeoniflorin content were measured based on the identification of ages. Different P. lactiflora root samples of different ages were collected from the four major growing areas in China: B...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22048

    authors: Zha LP,Cheng ME,Peng HS

    更新日期:2012-09-01 00:00:00

  • Response of donor and recipient cells after transplantation of cells to the ligament and tendon.

    abstract::The mechanical properties of healing ligaments and tendons are not comparable to those of normal tissue. To improve the quality of the ligament healing, therapeutic strategies include gene transfer or placement of mesenchymal stem cells at the healing site. Studies show that marker genes, growth factors, and antisense...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.10114

    authors: Hildebrand KA,Jia F,Woo SL

    更新日期:2002-07-01 00:00:00

  • Cytochemical techniques for zinc and heavy metals localization in nerve cells.

    abstract::Zinc is one of the most abundant oligoelements in the living cell. It appears tightly bound to metallothioneins, loosely bound to some metalloproteins and nucleic acids, or even as free ion. Small amounts of zinc ions (in the nanomolar range) regulate a plentitude of enzymatic proteins, receptors, and transcription fa...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.10037

    authors: López-García C,Varea E,Palop JJ,Nacher J,Ramirez C,Ponsoda X,Molowny A

    更新日期:2002-03-01 00:00:00

  • Natriuretic peptides in ectopic myocardial tissues originating from mouse embryonic stem cells.

    abstract::In a previous report we described the survival and contractile function of mouse embryonic stem cell-derived cardiomyocytes in the host retroperitoneum. To further understand the nature of embryonic stem cell-derived cardiomyocytes, the study assessed the synthesis of natriuretic peptides in ectopic myocardial tissues...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20152

    authors: Johkura K,Cui L,Yue F,Nitta K,Takei S,Okouchi Y,Asanuma K,Ogiwara N,Sasaki K

    更新日期:2005-03-01 00:00:00

  • Effects of aerobic training, resistance training, or combined resistance-aerobic training on the left ventricular myocardium in a rat model.

    abstract::This study follows the left ventricular (LV) hypertrophy in rats undergoing aerobic training alone (A), resistance training alone (R), or combined resistance and aerobic training (RA) (usually referred as concurrent training) program. A sedentary control group (C) was included. LV remodeling was evaluated using electr...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22394

    authors: De Souza MR,Pimenta L,Pithon-Curi TC,Bucci M,Fontinele RG,De Souza RR

    更新日期:2014-09-01 00:00:00

  • Effects of an endodontic auxiliary chemical substance on the bond strength of two methacrylate-based endodontic sealers to dentin.

    abstract:PURPOSE:To evaluate the influence of irrigation protocols and drying procedures on the bond strength of two methacrylate-based sealers to dentin and the associated failure pattern using scanning electron microscopy (SEM). MATERIALS AND METHODS:Fifty single-rooted human teeth were prepared with an irrigation protocol u...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22839

    authors: Fuzinatto RN,Farina AP,Souza MA,Miyagaki DC,Randi Ferraz CC,Cecchin D

    更新日期:2017-06-01 00:00:00

  • Effect of Aloe vera application on the content and molecular arrangement of glycosaminoglycans during calcaneal tendon healing.

    abstract::Although several treatments for tendon lesions have been proposed, successful tendon repair remains a great challenge for orthopedics, especially considering the high incidence of re-rupture of injured tendons. Our aim was to evaluate the pharmacological potential of Aloe vera on the content and arrangement of glycosa...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22422

    authors: Aro AA,Esquisatto MA,Nishan U,Perez MO,Rodrigues RA,Foglio MA,Carvalho JE,Gomes L,Vidal Bde C,Pimentel ER

    更新日期:2014-12-01 00:00:00