Image processing techniques in a preliminary morphometric characterization of corneal epithelium by scanning electron microscopy.

abstract：:Cartilage morphogenesis is a prerequisite for skeletal development and maintenance. The morphogenesis of cartilage determines the shape of bones, and joints including articular cartilage, ligaments, and tendon. This article reviews the recent advances in cartilage-derived morphogenetic proteins (CDMPs) and related bon...

journal_title：Microscopy research and technique

authors： Reddi AH

更新日期：1998-10-15 00:00:00

abstract：:This work provides examples of some of the imaging capabilities of environmental scanning electron microscopy applied to easily charged samples relevant to particle analysis. Environmental SEM (also referred to as high pressure or low vacuum SEM) can address uncoated samples that are known to be difficult to image. Mo...

journal_title：Microscopy research and technique

authors： Wight SA,Zeissler CJ

更新日期：1993-08-01 00:00:00

abstract：:The mechanical properties of healing ligaments and tendons are not comparable to those of normal tissue. To improve the quality of the ligament healing, therapeutic strategies include gene transfer or placement of mesenchymal stem cells at the healing site. Studies show that marker genes, growth factors, and antisense...

journal_title：Microscopy research and technique

authors： Hildebrand KA,Jia F,Woo SL

更新日期：2002-07-01 00:00:00

abstract：:Using the optical methods described, phase specimens can be observed with a modified light microscope in enhanced clarity, purified from typical artifacts which are apparent in standard phase contrast illumination. In particular, haloing and shade-off are absent, lateral and vertical resolution are maximized and the i...

journal_title：Microscopy research and technique

authors： Piper T,Piper J

更新日期：2013-10-01 00:00:00

abstract：:The synaptic organization of the amphibian sympathetic ganglia was studied, especially in the last two abdominal paravertebral ganglia of the frog. These ganglia appear to form a monosynaptic relay, not containing interneurons. They consist of two systems working in parallel: the principal neurons, by far the most num...

journal_title：Microscopy research and technique

authors： Lascar G,Eugene D,Taxi J

更新日期：1996-10-01 00:00:00

abstract：:Actin, though highly conserved, exhibits a myriad of diverse functions, most of which ultimately depend on its intrinsic ability to rapidly assemble and disassemble filamentous structures. Many organisms synthesize multiple actin isoforms even within the same cell. Tissue-specific expression patterns and tight develop...

journal_title：Microscopy research and technique

authors： Schoenenberger CA,Steinmetz MO,Stoffler D,Mandinova A,Aebi U

更新日期：1999-10-01 00:00:00

abstract：:Programmed cell death occurs in a variety of organs during amphibian metamorphosis and is usually identified by electron microscopy as apoptosis or its modifications. Because of the massive cell death that occurs during a short period, amphibian organs serve as an ideal model system for the study of mechanisms underly...

journal_title：Microscopy research and technique

authors： Ishizuya-Oka A

更新日期：1996-06-15 00:00:00

abstract：:Human monocytic leukemia U937 cells readily undergo apoptosis when exposed to various stimuli, including inhibition of protein synthesis, oxidative stress, antitumoral agents, etc. The sequential, step-by-step morphological changes in U937 cells that occur during the apoptotic program are largely determined by the act...

journal_title：Microscopy research and technique

authors： Abbro L,Lanubile R,Dini L

更新日期：2004-05-01 00:00:00

abstract：:Voltage-gated sodium channels are largely localized to the nodes of Ranvier in myelinated axons, providing the physiological basis for saltatory conduction. Studies using antisodium channel antibodies have shown that along demyelinated axons sodium channels form new distributions. The nature of this changed distributi...

journal_title：Microscopy research and technique

authors： England JD,Levinson SR,Shrager P

更新日期：1996-08-01 00:00:00

abstract：:We compared Hall's peak to continuum ratio method with a peak ratio method in order to quantify light elements (C, N, and O) in organic specimens as a model for biological thin sections. X-ray spectra were recorded by an energy dispersive X-ray spectrometer equipped with an ultra thin window detector. Spectra were pro...

journal_title：Microscopy research and technique

authors： Laquerriere P,Banchet V,Michel J,Zierold K,Balossier G,Bonhomme P

更新日期：2001-01-15 00:00:00

abstract：:Infection of humans with Helicobacter pylori results in the development of chronic gastritis and plays an important role in gastric ulcer pathogenesis. Despite the infiltration of the mucosa with specific immunocompetent cells and production of specific antibodies, the infection usually persists for life. This study w...

journal_title：Microscopy research and technique

authors： Jakob B,Birkholz S,Schneider T,Duchmann R,Zeitz M,Stallmach A

更新日期：2001-06-15 00:00:00

abstract：:Transmission X-ray microscopy (TXM) is a rapidly developing technique with the capability of nanoscale three dimensional (3D) real-space imaging. Combined with the wide range in energy tunability from synchrotron sources, TXM enables the retrieval of 3D microstructural information with elemental/chemical sensitivity t...

journal_title：Microscopy research and technique

authors： Kao TL,Shi CY,Wang J,Mao WL,Liu Y,Yang W

更新日期：2013-11-01 00:00:00

abstract：:Among the five senses, the sense of smell (olfaction) is the most sensitive and emotional window on the outside world (Stern and Marx, 1999). The olfactory system recognizes and discriminates myriad odorants of diverse molecular structures. What makes the olfactory system so specific and sensitive? OE harboring the ol...

journal_title：Microscopy research and technique

authors： Nibu K

更新日期：2002-08-01 00:00:00

abstract：:3D surface profiling and high resolution imaging were performed to refine the Florin rings and epicuticular wax crystals of Pinus koraiensis needles. Needles were collected from four-year-old seedlings and air-dried for surface observations. Field emission scanning electron microscopy revealed that stomata were found ...

journal_title：Microscopy research and technique

authors： Kim KW,Lee ST,Bae SW,Kim PG

更新日期：2011-12-01 00:00:00

abstract：:This experiment was conducted in Ile-de-France adult rams to examine the target point of a 2-month light cycle regimen on seminiferous tubule functions, on intertubular compartment and on Leydig cell parameters. Eight rams were subjected to a 2-month light cycle regimen and were compared to sexually active or inactive...

journal_title：Microscopy research and technique

authors： Hochereau-de Reviers MT,Perreau C,Pisselet C,Pelletier J

更新日期：1992-02-01 00:00:00

abstract：:Spermatogenesis is a process of division and differentiation by which spermatozoa are produced in seminiferous tubules. A measure of efficiency of spermatogenesis is the estimated number of spermatozoa produced per day per gram of testicular parenchyma. This measure is not influenced by species differences in testicul...

journal_title：Microscopy research and technique

authors： Johnson L

更新日期：1995-12-01 00:00:00

abstract：:The aqueous extract of betel nut (AEBN) induces the formation of preneoplastic nodules in the liver of Swiss Albino mice and leads to increased predisposition to cancer when administered transgenerationally. The aim of this investigation was to elucidate the alterations in ultrastructure of subcellular organelles in t...

journal_title：Microscopy research and technique

authors： Choudhury Y,Sharan RN

更新日期：2010-05-01 00:00:00

abstract：:Gymnosporia arenicola Jordaan (Celastraceae) is a shrub or small tree, which naturally occurs in coastal sand dunes of Southern Mozambique and South Africa. Its dried leaf is often used in traditional medicine for the treatment of infectious and inflammatory diseases. Hereby, we present results of studies carried out ...

journal_title：Microscopy research and technique

authors： Da Silva G,Serrano R,Gomes ET,Silva O

更新日期：2015-11-01 00:00:00

abstract：:This paper presents a snapshot view of the influence and direction of microcomputer technology for image analysis techniques in diagnostic pathology. Microcomputers have had considerable impact in bringing image analysis to wider application. Semi-automated tracing techniques are a simple means of providing objective ...

journal_title：Microscopy research and technique

authors： Jarvis LR

更新日期：1992-06-01 00:00:00

abstract：:Ultra-thin frozen sections are ideal substrates for immunolabelling in high resolution electron microscopy. However, visualization of subcellular structures is inferior to that obtained with corresponding plastic sections. Although negative staining is generally effective and even superior to positive staining, the ac...

journal_title：Microscopy research and technique

authors： Himmelhoch S

更新日期：1994-09-01 00:00:00

abstract：:Malaria is a serious worldwide disease, caused by a bite of a female Anopheles mosquito. The parasite transferred into complex life round in which it is grown and reproduces into the human body. The detection and recognition of Plasmodium species are possible and efficient through a process called staining (Giemsa). T...

journal_title：Microscopy research and technique

authors： Abbas N,Saba T,Rehman A,Mehmood Z,Javaid N,Tahir M,Khan NU,Ahmed KT,Shah R

更新日期：2019-07-01 00:00:00

abstract：:An understanding of the internal morphology of the ant mandible is important in explaining the relationship between the exocrine system and the behavioral and phylogenetic characteristics of different subfamilies of Formicidae. In this study, we investigated the occurrence of intramandibular glands in ants from the Po...

journal_title：Microscopy research and technique

authors： Martins LC,Serrão JE

更新日期：2011-08-01 00:00:00

abstract：:The dynamic transmission electron microscope (DTEM) is introduced as a novel tool for in situ processing of materials. Examples of various types of dynamic studies outline the advantages and differences of laser-based heating in the DTEM in comparison to conventional (resistive) heating in situ TEM methods. We demonst...

journal_title：Microscopy research and technique

authors： Taheri ML,Lagrange T,Reed BW,Armstrong MR,Campbell GH,DeHope WJ,Kim JS,King WE,Masiel DJ,Browning ND

更新日期：2009-03-01 00:00:00

abstract：:The increasing importance of in vitro models has presented new challenges in SEM processing techniques. The present study has evaluated the quality of preservation of cultured human lens epithelial cells processed by critical point, Peldri II, and tert-butyl alcohol drying. Specimens processed by critical point drying...

journal_title：Microscopy research and technique

authors： Cantu-Crouch D,Howe WE,McCartney MD

更新日期：1995-04-01 00:00:00

abstract：:In conventional whole-tooth culture systems, limitation exists regarding maintenance of the vitality of the dental pulp, because this tissue is encased in rigid dentin walls that hinder nutrition supply. We here report a whole tooth-in-jaw-bone culture system of rat mandibular first molars, where transcardiac perfusio...

journal_title：Microscopy research and technique

authors： Chokechanachaisakul U,Kaneko T,Yamanaka Y,Okiji T,Suda H

更新日期：2012-10-01 00:00:00

abstract：:The cyclic fluctuations in circulating levels of 17 beta-estradiol and progesterone that occur during the menstrual or estrous cycle are responsible for dramatic, cyclic changes in the epithelial lining and secretory status of the mammalian oviduct. The timely transition in the synthesis and release of oviduct protein...

journal_title：Microscopy research and technique

authors： Murray MK,DeSouza MM,Messinger SM

更新日期：1995-08-15 00:00:00

abstract：:Number of molecules and Brightness (N&B) has been proposed for measuring the molecular brightness and number of fluorophores in time-sequence of images, in live cells. If the fluorescently tagged-proteins are mobile in the illumination volume, the stoichiometry of their oligomers can be derived from the increase of th...

journal_title：Microscopy research and technique

authors： Trullo A,Corti V,Arza E,Caiolfa VR,Zamai M

更新日期：2013-11-01 00:00:00

abstract：:Several models for preparing and isolating human and animal gallbladder epithelial cells, including low-grade gallbladder carcinoma cells, as well as proposed systems for culturing these isolated epithelial cells are reviewed here. Several reports concerning tissue culture of the gallbladder are also reviewed. The cel...

journal_title：Microscopy research and technique

authors： Nakanuma Y,Katayanagi K,Kawamura Y,Yoshida K

更新日期：1997-10-01 00:00:00

abstract：:Effects of microstructure and phase component on mechanical property of spearer propodus of mantis shrimp were investigated. The spearer propodus consisted of three layers including epicuticle (outer layer), exocuticle (middle layer), and endocuticle (inner layer). The outer layer was composed of fluorapatite, which w...

journal_title：Microscopy research and technique

authors： Zhao Q,Chang Y,Lin Z,Zhang Z,Han Z,Ren L

更新日期：2020-09-16 00:00:00

abstract：:Adrenomedullin (AM) is a novel neuropeptide with special significance in the mammalian hypothalamo-hypophysial axis. By using an antiserum specific for human AM, we have studied the localization of AM-like immunoreactive (AMi) cell bodies and fibers in the hypothalamus and hypophysis of the amphibians Rana perezi (anu...

journal_title：Microscopy research and technique

authors： Muñoz M,López JM,Sánchez-Camacho C,Moreno N,Crespo M,González A

更新日期：2001-08-01 00:00:00