Abstract:
:Calorie restriction (CR) is known to delay the aging process in rodents and is postulated to act by decreasing free radical generation and increasing antioxidant enzyme activity. The present study was designed to investigate the effect of CR and age on oxidative stress-induced apoptosis and associated changes in the levels of TNF-alpha, and Bcl-2 in splenic T lymphocytes. Ad libitum (AL)- or CR-fed C57BL/6J mice were sacrificed either at 6 (young) or 18 (old) months and splenic lymphocytes were incubated with or without 25 micro M H2O2 to induce apoptosis. Apoptosis increased with age in cells of AL-fed mice incubated with H2O2. CR prevented this rise in apoptosis in total splenic lymphocytes and in CD4(+) and CD8(+) T lymphocyte subsets either with or without H2O2. Free radicals increased and mitochondrial membrane potential decreased in aged mice. CR prevented these changes and also prevented the age-associated increase in TNF-alpha and loss of Bcl-2 in total splenic lymphocytes and in CD4(+) and CD8(+) lymphocyte subsets. In summary, lymphocytes in aged AL-fed mice were much more susceptible to oxidative stress-induced apoptosis whereas CR normalized apoptosis by preventing the increase in TNF-alpha and the decrease in Bcl-2 associated with aging.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Avula CP,Fernandes Gdoi
10.1002/jemt.10206keywords:
subject
Has Abstractpub_date
2002-11-15 00:00:00pages
282-92issue
4eissn
1059-910Xissn
1097-0029journal_volume
59pub_type
杂志文章abstract::This study represents the first attempt to describe the ultra-structural and histological features of different three age-stages (young, mature, and adult) of rock pigeon Columba livia dakhlae captured from Egypt. The elongated tongue with round lingual apex carried a lingual nail rostrally on lingual tip. The current...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23394
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVES:To examine different types of restorative materials used in children as well as primary and permanent teeth enamel when affected by erosive foods. MATERIALS AND METHOD:Buttermilk, fruit yoghurt, Coca-cola, fruit juice, Filtek Z-250, Dyract Extra, Fuji II LC, and Fuji IX and tooth enamel were used. Measureme...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22315
更新日期:2014-01-01 00:00:00
abstract::To investigate intrinsic physicochemical properties and interactions of three different calcium hydroxide-based medications via means of different analytical methods. Two-commercial premixed medications: TempCanal(TCmx) and ProCalR(PCmx) and powder-form ProCal(PCpw) with glycerin were used. Vibrational modes were anal...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23600
更新日期:2020-09-21 00:00:00
abstract::The possibility that tannic acid and other classical mordants can be used in the electron microscopical section staining technique has been tested. A mordant can be defined as a chemical that combines with both a certain specific tissue component and the staining substance and thereby permits a staining reaction that ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070210110
更新日期:1992-03-01 00:00:00
abstract::In a previous report we described the survival and contractile function of mouse embryonic stem cell-derived cardiomyocytes in the host retroperitoneum. To further understand the nature of embryonic stem cell-derived cardiomyocytes, the study assessed the synthesis of natriuretic peptides in ectopic myocardial tissues...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20152
更新日期:2005-03-01 00:00:00
abstract::The morphology and ultrastructure of the rectum in Poecilimon cervus Karabağ, 1950 (Orthoptera, Tettigoniidae) were analyzed by light microscope, scanning (SEM) and transmission electron microscopes (TEM). The rectum is the final part of the digestive tract that plays an important role in water reabsorption in insects...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22787
更新日期:2017-02-01 00:00:00
abstract::This manuscript reviews gap junctions' roles in control of intestinal motility. Gap junctions (GJs) of small intestine (SmIn) are found between circular muscle (CM) cells, between interstitial cells of Cajal (ICC) of deep muscular plexus (DMP) and between them and adjacent outer circular muscle (OCM). GJs between long...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19991201)47:5<309::AID-JEM
更新日期:1999-12-01 00:00:00
abstract::The fixed number years-identification of Chinese material medicinal was a difficulty in the process of the traditional Chinese pharmacology, the phenomenon was found in our study of telomerase that the speed of the vascular cambium outside expanding growth fluctuated in a definite value. Based on it, we put forward a ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22154
更新日期:2013-02-01 00:00:00
abstract::The neurotrophins are a diverse family of peptides which activate specific tyrosine kinase-linked receptors. Over the past five decades, since the pioneering work of Levi-Montalcini and colleagues, the critical role that neurotrophins play in shaping the developing nervous system has become increasingly established. T...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19990515/01)45:4/5<276::AI
更新日期:1999-05-15 00:00:00
abstract::When 20-day-old rats are placed on a vitamin A deficient diet (VAD) for a period of 10 weeks, the seminiferous tubules are found to contain only Sertoli cells, a few residual A0, A1 spermatogonia, and preleptotene spermatocytes (PL). The type A1 spermatogonia and PL spermatocytes are arrested in their G2 phase. In VAD...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070200106
更新日期:1992-01-01 00:00:00
abstract::Microscopic magnetic resonance imaging (µMRI) T2 data from canine cartilage at different tibial locations were analyzed to investigate the influences of spatial resolution and pixel position on the T2 sensitivity to osteoarthritis (OA). Five experimental factors were investigated: inaccurate pixel position, different ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22694
更新日期:2016-08-01 00:00:00
abstract::This study evaluated the effect of chitosan, zirconium oxide, and hydroxyapatite on the apatite-like forming ability, porosity, and bond-strength of calcium-aluminate cements (C). Three hundred bovine root-slices were assigned to one of five groups, according to the material: MTA, C, C + chitosan (Cchi), C + zirconium...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23678
更新日期:2020-12-22 00:00:00
abstract::This experiment was conducted in Ile-de-France adult rams to examine the target point of a 2-month light cycle regimen on seminiferous tubule functions, on intertubular compartment and on Leydig cell parameters. Eight rams were subjected to a 2-month light cycle regimen and were compared to sexually active or inactive...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070200306
更新日期:1992-02-01 00:00:00
abstract::Antlion (Myrmeleon sagax) larvae live in sandy soil and possess the ability to enter soil quickly. In this article, the hierarchical structure of the nonsmooth surface of antlion larvae was obtained using a scanning electron microscope (SEM). Based on the results, a bionic nonsmooth structure model was established to ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23419
更新日期:2020-04-01 00:00:00
abstract::Ensheathing cells are the glial cells that envelop olfactory axons as they course from the olfactory epithelium to the bulb. They are derived from the olfactory placode and differ from the typical glia in terms of sharing the phenotypes of both astrocytes and Schwann cells. The aims of this study are to review (1) cel...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10151
更新日期:2002-08-01 00:00:00
abstract::In spite of accumulating evidence for a modulation of sympathetic neurotransmission by endogenously produced nitric oxide (NO), it remains unclear in which parts of the vascular system and at what level this interaction takes place. The aim of the present study was to investigate the distribution of endothelial and ne...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070290214
更新日期:1994-10-01 00:00:00
abstract::The aim of this study was to make a comparative evaluation of the surface topography of the cervical third and tip of four different rotary systems, before and after being used 12 times, in resin blocks with simulated root canals with standardized 45° curvatures, and analyzed by scanning electronic microscopy (SEM). A...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22139
更新日期:2013-01-01 00:00:00
abstract::Currently, exploration of alternative energy resources is hotly debated among the scientific community owing to rising energy crises and environmental issues. Biodiesel, as renewable energy source proves to be a better option and substitute to petro diesel. In this regard, nonedible seeds could be a better feedstock f...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23265
更新日期:2019-07-01 00:00:00
abstract::Sequences highly similar (>95%) to the mouse mammary tumor virus (MMTV) env gene have been amplified from human DNA samples, including DNA samples from patients with breast cancer (BC) and persons who did not have BC. The sequences from human DNA were distinct from the MMTV sequences used as controls in these PCR reac...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20233
更新日期:2005-11-01 00:00:00
abstract::This article reviews the distribution of S100 proteins in the human peripheral nervous system. The expression of S100 by peripheral glial cells seems to be a distinctive fact of these cells, independently of their localization and their ability to myelinate or not. S100 proteins expressing cells include satellite cell...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10304
更新日期:2003-04-15 00:00:00
abstract::The properties of the postganglionic sympathetic neurones supplying the heart and arising in the stellate and adjacent paravertebral ganglia of various species are discussed with respect to their location, morphology, synaptic input and membrane characteristics. Results from our laboratory on the morphology of rat ste...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19960901)35:1<69::AID-JEMT
更新日期:1996-09-01 00:00:00
abstract::A new and improved technique for microvascular corrosion casting was developed and verified by examination of corrosion casts of 90 bovine limbs. The described technique renders a complete filling of the vasculature of the claw even in regions that hitherto proved to be difficult regarding completeness of filling such...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990501)45:3<184::AID-JEM
更新日期:1999-05-01 00:00:00
abstract::Photoconversion of fluorescent staining into stable diaminobenzidine (DAB) precipitate is widely used for neuroanatomical and developmental studies. An important advantage of the approach is to make correlations between light and electron microscopy analyses possible, the DAB reaction product formed during photoconver...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20239
更新日期:2005-10-01 00:00:00
abstract::The adaptations of the mouse uterus to pregnancy include extensive modifications of the cells and extracellular matrix of the endometrial connective tissue that surround the embryos. Around each implanted embryo this tissue redifferentiates into a transient structure called decidua, which is formed by polygonal cells ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20265
更新日期:2006-01-01 00:00:00
abstract::An understanding of the internal morphology of the ant mandible is important in explaining the relationship between the exocrine system and the behavioral and phylogenetic characteristics of different subfamilies of Formicidae. In this study, we investigated the occurrence of intramandibular glands in ants from the Po...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20956
更新日期:2011-08-01 00:00:00
abstract::The grain boundary healing behavior of crushed rock salt was mainly studied by employing the environmental scanning electron microscope (ESEM) to study the consolidation mechanism of rock salt backfill. Dedicated miniature round rock salt specimens were prepared for observation of the water trapping effect by using a ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070250517
更新日期:1993-08-01 00:00:00
abstract::Contraction of muscular tissue requires the synchronized shortening of myofibers arrayed in complex geometrical patterns. Imaging such myofiber patterns with diffusion-weighted MRI reveals architectural ensembles that underlie force generation at the organ scale. Restricted proton diffusion is a stochastic process res...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.22777
更新日期:2018-02-01 00:00:00
abstract::Rhus succedanea is generally traded, distributed, and sold in the markets in its crude and raw form. This may have been mixed with adulterants, mismanaged by malpractices, and substituted with other closely related drugs having different effect. This study is therefore carried out to authenticate the plant through pha...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23367
更新日期:2019-12-01 00:00:00
abstract::The imaging and characterization of dislocations is commonly carried out by thin foil transmission electron microscopy (TEM) using diffraction contrast imaging. However, the thin foil approach is limited by difficult sample preparation, thin foil artifacts, relatively small viewable areas, and constraints on carrying ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20293
更新日期:2006-05-01 00:00:00
abstract::From the first cases of bovine spongiform encephalopathy (BSE) among cattle in the United Kingdom in 1986, the route of infection of BSE is generally believed by means of feeds containing low level of processed animal proteins (PAPs). Therefore, many feed bans and alternative and complementary techniques were resulted...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20951
更新日期:2011-08-01 00:00:00