Abstract:
:Neurons begin to polarize when one of the neurites becomes the axon. Hippocampal neurons in cell culture have a sharp transition between their unpolarized and polarized stage revealed by the rapid growth of the future axon. Recent progress shows that both a cytoplasmic membrane flow and actin dynamics govern axon formation, and thereby initial neuronal polarization. We here review these mechanisms, evaluate their physiological role, and show similarities to the transient polarization of migrating fibroblasts. Finally, we present a model how actin dynamics and vectorial membrane flow may interact to achieve axon formation.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Bradke F,Dotti CGdoi
10.1002/(SICI)1097-0029(20000101)48:1<3::AID-JEMT2keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
3-11issue
1eissn
1059-910Xissn
1097-0029pii
10.1002/(SICI)1097-0029(20000101)48:1<3::AID-JEMT2journal_volume
48pub_type
杂志文章,评审abstract::Proliferative activity of tumors is strongly associated with prognosis and response to therapy. The reason for faster and uncontrolled growth rate of tumors compared with normal tissue may be caused by the greater proliferation of cells, the smaller rate of cell death, or both. Cell production vs. cell loss rates, and...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980301)40:5<408::AID-JEM
更新日期:1998-03-01 00:00:00
abstract::Focused ion beam (FIB) techniques are among the most important tools for the nanostructuring of surfaces. We used the FIB/SEM (scanning electron microscope) for milling and imaging of digestive gland cells. The aim of our study was to document the interactions of FIB with the surface of the biological sample during FI...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20494
更新日期:2007-10-01 00:00:00
abstract::All organisms exhibit significant daily rhythms in a myriad of functions from molecular levels to the level of the whole organism. Significantly, most of these rhythms will persist under constant conditions, showing that they are driven by an internal circadian clock. In birds the circadian system is composed of sever...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1068
更新日期:2001-04-01 00:00:00
abstract::Amphibia Kupffer cells (i.e., liver resident macrophages) show many common characteristics when compared with Mammalia Kupffer cells: filopodia, microvillous-like structures, lamellipodia, fuzzy coat, coated vesicles, bristled vacuoles, nonspecific esterase activity, and pinocytotic and phagocytic activity are present...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10101
更新日期:2002-06-15 00:00:00
abstract::In this paper, an application of contact microradiography with soft X-rays for detecting the uptake site of heavy metal in the whole plant leaves is investigated. The X-ray source is a laser-plasma one based on an Nd:glass laser. The soft X-ray radiation emitted from the plasma laser targets of magnesium, iron, and co...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20350
更新日期:2006-08-01 00:00:00
abstract::Correlative microscopy has become increasingly important for the analysis of the structure, function, and dynamics of cells. This is largely due to the result of recent advances in light-, probe-, laser- and various electron microscopy techniques that facilitate three-dimensional studies. Furthermore, the improved und...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20408
更新日期:2007-03-01 00:00:00
abstract::The molecular basis of hearing is less well understood than many other senses. However, recent studies in Drosophila have provided some important steps towards a molecular understanding of hearing. In this report, we summarize these findings and their implications on the relationship between hearing and touch. In Dros...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20053
更新日期:2004-04-15 00:00:00
abstract::Parathyroid glands (n = 271) removed from 130 patients were examined by light and electron microscopy. A standardized method of tissue processing was employed and morphometry was performed. The aim of the paper is to provide a description of the human parathyroid chief cell ultrastructure in health and disease, with q...
journal_title:Microscopy research and technique
pub_type: 杂志文章,多中心研究
doi:10.1002/jemt.1070320210
更新日期:1995-10-01 00:00:00
abstract::This study follows the left ventricular (LV) hypertrophy in rats undergoing aerobic training alone (A), resistance training alone (R), or combined resistance and aerobic training (RA) (usually referred as concurrent training) program. A sedentary control group (C) was included. LV remodeling was evaluated using electr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22394
更新日期:2014-09-01 00:00:00
abstract::Changes in the intracellular Ca2+ concentration ([Ca2+]i) within CA1 hippocampal pyramidal neurons were imaged using confocal laser scanning microscopy in conjunction with Ca(2+)-sensitive fluorescent indicators. The imaging was performed in thick hippocampal brain slices while simultaneously measuring or controlling ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070290404
更新日期:1994-11-01 00:00:00
abstract::With an increase in the advancement of digital imaging and computing power, computationally intelligent technologies are in high demand to be used in ophthalmology cure and treatment. In current research, Retina Image Analysis (RIA) is developed for optometrist at Eye Care Center in Management and Science University. ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22867
更新日期:2017-07-01 00:00:00
abstract::Effects of microstructure and phase component on mechanical property of spearer propodus of mantis shrimp were investigated. The spearer propodus consisted of three layers including epicuticle (outer layer), exocuticle (middle layer), and endocuticle (inner layer). The outer layer was composed of fluorapatite, which w...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23598
更新日期:2020-09-16 00:00:00
abstract::Physiological oral mechanical forces may play a role on the progression of enamel carious lesions to cavitation. Thus, the aim of this study was to describe, by 3D finite element analysis, stress, and strain patterns in sound and carious enamel after a simulated occlusal load. Micro-CT based models were created and me...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22549
更新日期:2015-10-01 00:00:00
abstract::This study was conducted to provide comprehensive information on the anatomical and histological features of the upper (UL) and lower (LL) lips of Rahmani sheep by gross examination, morphometric analysis in addition to Light and scanning electron microscope (SEM). Samples from normal healthy adult male sheep heads we...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23660
更新日期:2020-12-01 00:00:00
abstract::Peroxisomes are essential organelles that may be involved in various functions, dependent on organism, cell type, developmental stage of the cell, and the environment. Until recently, peroxisomes were viewed as a class of static organelles that developed by growth and fission from pre-existing organelles. Recent obser...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10323
更新日期:2003-06-01 00:00:00
abstract::The present study focused to elucidate the promising role of probiotics in the fish culture, by employing microscopic and biochemical parameters to rationalize the possible outcomes. After preliminary feeding trial histopathological analysis revealed clear and visible changes in the intestinal and liver cells of CTRL ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23416
更新日期:2020-03-01 00:00:00
abstract::In certain cases of infertility in domestic species, and in the homozygous c-ros tyrosine kinase knockout mouse, males are infertile as a result of unopposed sperm swelling. This induces flagellar angulation, preventing their normal migration in the female tract. Angulation always occurs at the site of the cytoplasmic...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10314
更新日期:2003-05-01 00:00:00
abstract::We measured cell membrane deformations under the modulation of piconewton magnetic force by using optical tracking and noninterferometric widefield optical profilometry. The magnetic force was applied to fibronectin-coated paramagnetic beads that bound to transmembrane protein integrins. At an image-acquisition rate o...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20593
更新日期:2008-08-01 00:00:00
abstract::Ascidian embryos are useful for examining how events that occur during fertilization and cleavage affect gastrulation because they gastrulate early in development, during the seventh cleavage. In ascidians, both dorsal-ventral and anterior-posterior axes are determined before first cleavage. The dorsal-ventral axis is...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070260403
更新日期:1993-11-01 00:00:00
abstract::We compared physiological and ultrastructural indices of acute lung injury (ALI) during septic shock caused by taxonomically diverse pathogens to distinguish ALI due to endogenous inflammatory mediators vs. microbial exotoxins or other factors. Conscious rats were infected i.v. with gram-negative Escherichia coli (EC,...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070260512
更新日期:1993-12-01 00:00:00
abstract::This study represents the first definitive anatomical description of the tongue and lingual papillae of the pampas deer and compares the different information on the morphology of the other ruminant species available in the literature. In this study, the tongues of four adult and one fetal deer were used. The tongue w...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22263
更新日期:2013-10-01 00:00:00
abstract::Scanning electron microscope images of complete and damaged frustules of Nitzschia marina Grun. revealed an unusual chambered structure of the valve. This structure is not present in any existing nitzschioid genus and warrants an independent generic status for this taxon, which is therefore separated into Alveus gen. ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(199601)33:1<2::AID-JEMT2>3
更新日期:1996-01-01 00:00:00
abstract::Epidemiologic studies have associated cardiovascular morbidity and mortality with ambient particulate air pollution. Particles smaller than 100 nm in diameter (ultrafine particles) are present in the urban atmosphere in very high numbers yet at very low mass concentration. Organs beyond the lungs are considered as tar...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20044
更新日期:2004-04-01 00:00:00
abstract::This study investigated the relationship between enteroendocrine and mucus-secreting cells distribution, the severity of colonic mucosal injury and intestinal motility in experimental colorectal carcinogenesis. Using a standardized murine model of colorectal carcinogenesis, eight-weeks-old female Wistar rats weighting...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22534
更新日期:2016-01-01 00:00:00
abstract::The adaptations of the mouse uterus to pregnancy include extensive modifications of the cells and extracellular matrix of the endometrial connective tissue that surround the embryos. Around each implanted embryo this tissue redifferentiates into a transient structure called decidua, which is formed by polygonal cells ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20265
更新日期:2006-01-01 00:00:00
abstract::High-angle annular dark-field or Z-contrast microscopy was used to demonstrate that well dispersed metal supported catalysts consist of nanometer sized clusters. Depending upon the impregnated metal, different cluster sizes were observed. Grouping of Pd clusters could also be confirmed by analytical electron microscop...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070280509
更新日期:1994-08-01 00:00:00
abstract::Pituitary-derived factors, including ACTH, have been widely implicated in initiating adrenal regeneration. However, recent work has demonstrated that adrenal regeneration is also modulated by adrenal nerves that extensively reinnervate the regenerating adrenal. Moreover, transection of the splanchnic nerve removes sen...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10334
更新日期:2003-06-15 00:00:00
abstract::The main purpose of this study was to compare the cyclic fatigue resistances of the three different nickel-titanium files in 2.5% sodium hypochlorite solution and distilled water at physiologic intracanal temperature (35°C). Three NiTi files such as One Curve (OC) (Micro-Mega, Besancon, France), OneShape (OS) (Micro-M...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23381
更新日期:2019-12-01 00:00:00
abstract::The present study provides experimental data which indicate that the neutrophil is ideal for studying programmed cell death or apoptosis in vitro. Neutrophils can be obtained from human peripheral blood in large numbers with minimal experimental manipulation and are easily separated from other leukocytes, providing ne...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070280408
更新日期:1994-07-01 00:00:00
abstract::Non-alcoholic fatty liver disease (NAFLD) is a clinical-pathological syndrome that includes a wide spectrum of morphological alterations. In research, animal models are crucial in evaluating not only the pathogenesis of NAFLD and its progression, but also the therapeutic effects of various agents. Investigations on th...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22401
更新日期:2014-10-01 00:00:00