Abstract:
:Basement membranes (BMs) were first described in the mid-19th century, but they were not isolated and prepared for compositional studies until nearly 100 years later. Early methods of isolation were carried out on renal glomeruli, which were first sub-fractionated from kidney tissues by sieving. BMs were then isolated from the glomeruli by ultrasonic disruption, which, following low speed centrifugation, yielded "purified" but highly fragmented BM material. In an effort to obviate the mechanical damage to BMs produced by ultrasound, a sequential detergent solubilization technique was introduced that resulted in morphologically intact BMs from a variety of tissue sub-fractions. This was highly advantageous because "acellular" BMs produced by the procedure could be examined critically by light and electron microscopic methods. Subsequently, this procedure has been utilized to demonstrate the substructural heterogeneity of vascular and non-vascular BMs from a wide variety of animal species. The current review describes the results of scanning and transmission electron microscopic studies of acellular BMs prepared from renal glomeruli and from the retinal microvessels of the eye. These BMs are of particular interest to basic scientists and clinicians because they are altered in several disease states, most notably diabetes mellitus. An effort is made to point out the implications of glomerular and retinal vessel BM changes to the pathogenesis of diabetic kidney and retinal vessel BM disease.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Carlson ECdoi
10.1002/jemt.1070280302subject
Has Abstractpub_date
1994-06-15 00:00:00pages
165-77issue
3eissn
1059-910Xissn
1097-0029journal_volume
28pub_type
杂志文章,评审abstract::In present study, multiple microscope techniques were used for the systematics identification of the species Asplenium dalhousiae. The plant was collected from different phytogeographical and its natural habitat of Pakistan, where it shows higher diversity. Morphology, foliar epidermal anatomy, and spore morphological...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23189
更新日期:2019-04-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22283
更新日期:2013-11-01 00:00:00
abstract::Chironomids have been widely used in environmental monitoring and toxicity assays. Assessment criteria for chironomids range from the molecular-biochemical level to the population level. However, the use of markers to evaluate cellular and anatomical changes in organs and systems of individuals under contaminant expos...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23278
更新日期:2019-08-01 00:00:00
abstract::Samples of polyglycolic acid PGA/polycaprolactone PCL copolymer absorbable suture materials, which is called Poliglecaprone PGC copolymer suture, were thermally treated by two different processes. The first method is the direct heat exposure, from 20 to 38 °C, in the surrounding medium. Which is an environmental study...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22957
更新日期:2018-01-01 00:00:00
abstract::Locusts are attractive model preparations for cellular investigations of neurodevelopment. In this study, we investigate the immunocytochemical localization of histamine in the developing ventral nerve cord of two locust species, Schistocerca gregaria and Locusta migratoria. Histamine is the fast neurotransmitter of p...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20980
更新日期:2011-10-01 00:00:00
abstract::The aim of this study was to make a comparative evaluation of the surface topography of the cervical third and tip of four different rotary systems, before and after being used 12 times, in resin blocks with simulated root canals with standardized 45° curvatures, and analyzed by scanning electronic microscopy (SEM). A...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22139
更新日期:2013-01-01 00:00:00
abstract::Classical conditioning (CC) with a sweet flavored is useful for experimental models of immune response. The objective this work was analyze spleen cell response on the differentiation of B-lymphocytes associated with the CC with sweet flavored in adult rats. Twelve adult male rats were divided (n = 3) in Group 1 (cont...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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更新日期:2018-12-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22401
更新日期:2014-10-01 00:00:00
abstract::Ketogenic diets (KDs) have shown beneficial effects in experimental models of neurodegeneration, designating aged individuals as possible recipients. However, few studies have investigated their consequences on aging brain. Here, late-adult rats (19 months of age) were fed for 8 weeks with two medium chain triglycerid...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20737
更新日期:2009-12-01 00:00:00
abstract::The adaptations of the mouse uterus to pregnancy include extensive modifications of the cells and extracellular matrix of the endometrial connective tissue that surround the embryos. Around each implanted embryo this tissue redifferentiates into a transient structure called decidua, which is formed by polygonal cells ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20265
更新日期:2006-01-01 00:00:00
abstract::In addition to pesticidal activity, the capacity of natural compounds to inhibit the reproduction of parasites emerge as an important alternative tick control method. In this context, Satureja spp. stand out due to their recognized pesticidal properties. Among parasites of veterinary importance, the cattle tick, Rhipi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23693
更新日期:2021-01-06 00:00:00
abstract::Low voltage electron microscopes working in transmission mode, like LVEM5 (Delong Instruments, Czech Republic) working at accelerating voltage 5 kV or scanning electron microscope working in transmission mode with accelerating voltage below 1 kV, require ultrathin sections with the thickness below 20 nm. Decreasing of...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22659
更新日期:2016-06-01 00:00:00
abstract::Oligodendrocytes (OL) play a significant physiological role in the central nervous system by creating the myelin sheath that allows for the efficient conduction of nerve impulses. Therefore, it is important to understand which signalling cascades define the proliferation, differentiation, survival, and myelin formatio...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1052
更新日期:2001-03-15 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22206
更新日期:2013-06-01 00:00:00
abstract::The imaging and characterization of dislocations is commonly carried out by thin foil transmission electron microscopy (TEM) using diffraction contrast imaging. However, the thin foil approach is limited by difficult sample preparation, thin foil artifacts, relatively small viewable areas, and constraints on carrying ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20293
更新日期:2006-05-01 00:00:00
abstract::This paper describes the use of a photobleach method and confocal microscopy to compare the cell-to-cell transfer rate of 5,6 carboxyfluorescein in dissociated embryonic chick lens cells with those in the anterior epithelium of the whole embryonic chick lens. The average cell-to-cell transfer rates obtained were 7.9 x...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070310508
更新日期:1995-08-01 00:00:00
abstract::Infection of humans with Helicobacter pylori results in the development of chronic gastritis and plays an important role in gastric ulcer pathogenesis. Despite the infiltration of the mucosa with specific immunocompetent cells and production of specific antibodies, the infection usually persists for life. This study w...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1111
更新日期:2001-06-15 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23152
更新日期:2019-02-01 00:00:00
abstract::Scanning electron microscope (SEM) investigations on the holdfast elements, proboscis hooks, and trunk spines of Dentitruncus truttae (Acanthocephala, Palaeacanthocephala), an endoparasite of Salmo trutta (brown trout), provide more data about the surface of these taxonomic relevant structures. In both acanthocephalan...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20543
更新日期:2008-03-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20130
更新日期:2004-12-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20025
更新日期:2004-02-15 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070280509
更新日期:1994-08-01 00:00:00
abstract::Several studies support the role of Western-style diet (WD) in inflammatory bowel disease (IBD). Toll-like receptors/NOD-like receptors (TLRs/NLRs) are important to maintain a healthy epithelium as well as inducing inflammation. Given that dietary factors influence IBD development, that epithelial dysfunction is thoug...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22831
更新日期:2017-06-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10019
更新日期:2002-01-15 00:00:00
abstract::Adrenomedullin (AM) is a novel neuropeptide with special significance in the mammalian hypothalamo-hypophysial axis. By using an antiserum specific for human AM, we have studied the localization of AM-like immunoreactive (AMi) cell bodies and fibers in the hypothalamus and hypophysis of the amphibians Rana perezi (anu...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1131
更新日期:2001-08-01 00:00:00
abstract::The new stereology permits the objective, quantitative description of morphology by efficient and design-based methods. Applications to placentas in normal and abnormal pregnancies have proved of great value for challenging earlier misconceptions and interpreting better the processes of growth, morphogenesis, adaptati...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970701/15)38:1/2<195::AI
更新日期:1997-07-01 00:00:00
abstract::Fluorescence correlation spectroscopy (FCS) has become an increasingly important measurement tool for biological and biomedical investigations, with the capability to assay molecular dynamics and interactions both in vitro and within living cells. Information recovery in FCS requires an accurate characterization and c...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20454
更新日期:2007-08-01 00:00:00
abstract::This paper describes four investigations of the olfactory mucosa of the brown trout: 1) the ultrastructure of the olfactory mucosa as revealed by scanning (SEM), conventional transmission (TEM), and high voltage (HVEM) electron microscopy; 2) light and electron-microscopic investigations of retrograde transport of the...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070230104
更新日期:1992-10-01 00:00:00
abstract::Among the five senses, the sense of smell (olfaction) is the most sensitive and emotional window on the outside world (Stern and Marx, 1999). The olfactory system recognizes and discriminates myriad odorants of diverse molecular structures. What makes the olfactory system so specific and sensitive? OE harboring the ol...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10130
更新日期:2002-08-01 00:00:00
abstract::Many aspects of the organization of the electromotor synapse of electric fish resemble the nerve-muscle junction. In particular, the postsynaptic membrane in both systems share most of their proteins. As a remarquable source of cholinergic synapses, the Torpedo electrocyte model has served to identify the most importa...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(20000401)49:1<73::AID-JEMT
更新日期:2000-04-01 00:00:00