GFAP immunoreactivity and transcription in trigeminal and dental tissues of rats and transgenic GFP/GFAP mice.

Abstract:

:Sensory mechanisms in teeth are not well understood and may involve pulpal-neural interactions. Tooth cells that proliferate in vitro have polyclonal immunoreactivity (IR) for glial fibrillary acidic protein (GFAP), growth-associated protein (GAP-43), and vimentin, plus glial-like ion channels. Here, we analyzed GFAP-IR patterns in dental and trigeminal tissues of rats, for comparison with green fluorescent protein (GFP) associated with GFAP transcription in transgenic mice, in order to better characterize glial-like cells in dental tissues. Astrocytes, ganglion satellite cells, and epineurial Schwann cells were demonstrated by anti-GFAP antibodies and GFP-GFAP, as expected. Odontoblasts did not stain by any of these methods and cannot be the glial-like cells. Fibroblasts and undifferentiated mesenchymal cells in pulp had polyclonal GFAP-IR and vimentin-IR, while nerve fibers reacted only with polyclonal antibody. Some Schwann cell subtypes in trigeminal nerve and oral mucosa were positive for GFP and for polyclonal anti-GFAP, but not for monoclonal antibody. In pulp almost all Schwann cells were unstained, but many Schwann cells in periodontal ligament had polyclonal GFAP-IR. These results show greater heterogeneity for Schwann cells than expected, and suggest that the glial-like pulp cells are fibroblasts and/or undifferentiated mesenchymal cells or stem cells. We also found that polyclonal GFAP revealed intermediate filaments in preterminal sensory nerve fibers, thereby providing a useful marker for that neural subregion. GFP transcription by some Schwann cell subtypes in oral mucosae and trigeminal nerve, but not trigeminal root was a novel finding that reveals more complexity in peripheral glia than previously recognized.

journal_name

Microsc Res Tech

authors

Byers MR,Maeda T,Brown AM,Westenbroek RE

doi

10.1002/jemt.20130

keywords:

subject

Has Abstract

pub_date

2004-12-01 00:00:00

pages

295-307

issue

6

eissn

1059-910X

issn

1097-0029

journal_volume

65

pub_type

杂志文章
  • SEM search for sound production and sound perception organs in a variety of Drosophila species.

    abstract::The discovery of new song types in Hawaiian Drosophila species has raised a question of alternative sound production and sound perception mechanisms in Drosophila. For example D. disjuncta males, which produce song with a carrier frequency of 5,000-6,000 Hz, do not vibrate their wings but only tremble them in a very s...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/1097-0029(20000715)50:2<161::AID-JEMT8>3.0

    authors: Hoikkala A,Moro S

    更新日期:2000-07-15 00:00:00

  • In situ hybridization in HIV research.

    abstract::In situ hybridization (ISH) for HIV is an arduous, demanding means of detecting viral genetic material in cells and tissues. Good ISH requires broad technical skills and devotion to controls for every step of the process as well as a critical eye when interpreting results. ISH may be used to detect HIV in three ways: ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1070250111

    authors: Fox CH,Cottler-Fox M

    更新日期:1993-05-01 00:00:00

  • Dynamic structural changes of synaptic contacts in the visual system of insects.

    abstract::The visual system of insects provides an excellent model to study processes of transduction and transmission of photic information, synaptogenesis, synaptic plasticity, and wiring between photoreceptors and their visual interneurons in the optic lobe. This review describes synaptic contacts between photoreceptors and ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.10141

    authors: Pyza E

    更新日期:2002-08-15 00:00:00

  • Müllerian inhibiting substance: new perspectives and future directions.

    abstract::MIS, as a differentiate and antiproliferative agent, is precisely regulated, for example, at the transcriptional level by such transacting factors as SRY, and posttranslationally by testosterone. Processing of MIS most likely requires an as yet unknown in vivo protease which probably serves to control cleavage of MIS ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1070250205

    authors: Catlin EA,MacLaughlin DT,Donahoe PK

    更新日期:1993-06-01 00:00:00

  • In vivo second-harmonic generation and ex vivo coherent anti-stokes raman scattering microscopy to study the effect of obesity to fibroblast cell function using an Yb-fiber laser-based CARS extension unit.

    abstract::Nonlinear microscopy techniques are being increasingly used to perform in vivo studies in dermatology. These methods enable us to investigate the morphology and monitor the physiological process in the skin by the use of femtosecond lasers operating in the red, near-infrared spectral range (680-1,300 nm). In this work...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22545

    authors: Haluszka D,Lőrincz K,Molnár G,Tamás G,Kolonics A,Szipőcs R,Kárpáti S,Wikonkál NM

    更新日期:2015-09-01 00:00:00

  • Imaging molecular structure and physiological function of gap junctions and hemijunctions by multimodal atomic force microscopy.

    abstract::Gap junctions are specialized plasma membrane structures that join neighboring cells via specialized intercellular ion channels (hemichannels) and provide a direct pathway for cell-cell communication. They presumably mediate regulation of growth, transmission of developmental signals, coordination of muscle contractio...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/1097-0029(20010201)52:3<273::AID-JEMT1013>

    authors: Lal R,Lin H

    更新日期:2001-02-01 00:00:00

  • Enhanced effects of nonisotopic hafnium chloride in methanol as a substitute for uranyl acetate in TEM contrast of ultrastructure of fungal and plant cells.

    abstract::This ultrastructural study showed that nonisotopic methanolic hafnium chloride and aqueous lead solution was an excellent new electron stain for enhancing TEM contrasts of fungal and plant cell structures. The ultrastructural definition provided by the new stain was often superior to that provided by conventional stai...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20964

    authors: Ikeda K,Inoue K,Kanematsu S,Horiuchi Y,Park P

    更新日期:2011-09-01 00:00:00

  • Imaging of three-dimensional epithelial architecture and function in cultured CaCo2a monolayers with two-photon excitation microscopy.

    abstract::The principal functions of the gastrointestinal tract mucosa include nutrient absorption, protein and fluid secretion, and the regulated symbiosis with intraluminal contents. Research in epithelial biology has benefited significantly from the use of cultured monolayer preparations, which closely replicate the structur...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/1097-0029(20001015)51:2<204::AID-JEMT11>3.

    authors: Gilbert RJ,Hoffman M,Capitano A,So PT

    更新日期:2000-10-15 00:00:00

  • Surface damage induced by FIB milling and imaging of biological samples is controllable.

    abstract::Focused ion beam (FIB) techniques are among the most important tools for the nanostructuring of surfaces. We used the FIB/SEM (scanning electron microscope) for milling and imaging of digestive gland cells. The aim of our study was to document the interactions of FIB with the surface of the biological sample during FI...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20494

    authors: Drobne D,Milani M,Leser V,Tatti F

    更新日期:2007-10-01 00:00:00

  • Stem cells in the development and differentiation of the human adrenal glands.

    abstract::There are no studies on stem cells (SCs) and development and differentiation (DD) of the human adrenal glands. The SCs in DD of the adrenal glands were herein investigated histochemically and immunohistochemically in 18 human embryonic adrenal glands at gestational week (GW) 7-40. At 7 GW, the adrenal glands were pres...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22445

    authors: Terada T

    更新日期:2015-01-01 00:00:00

  • Immune response to autologous and heterologous Helicobacter pylori antigens in humans.

    abstract::Infection of humans with Helicobacter pylori results in the development of chronic gastritis and plays an important role in gastric ulcer pathogenesis. Despite the infiltration of the mucosa with specific immunocompetent cells and production of specific antibodies, the infection usually persists for life. This study w...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1111

    authors: Jakob B,Birkholz S,Schneider T,Duchmann R,Zeitz M,Stallmach A

    更新日期:2001-06-15 00:00:00

  • Sympathetic neuromuscular transmission at a varicosity in a syncytium.

    abstract::The autonomic neuromuscular junction at a varicosity in the vas deferens is defined by the localization of the vesicle-associated protein syntaxin in high concentrations in the axolemma and a high density of P2x1 receptors in a cluster beneath the varicosity. Calcium fluxes have been observed in all individual varicos...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19980915)42:6<433::AID-JEM

    authors: Bennett MR,Cheung A,Brain KL

    更新日期:1998-09-15 00:00:00

  • Peribronchiolar accumulation of dendritic cells and their close association with CD4(+) T cells in the murine lung hypersensitivity.

    abstract::In order to understand the interaction between dendritic cells (DCs) and helper T (Th) cells in the region exposed to antigens during pulmonary delayed-type hypersensitivity (DTH), which is considered to be mediated by Th1 cells, we immunohistochemically investigated their spatial relationship in the cellular infiltra...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1091

    authors: Nishida T,Inoue K,Kawata Y,Suzuki H,Kinoshita H,Kaneda K

    更新日期:2001-05-15 00:00:00

  • Fluorescent human lung macrophages analyzed by spectral confocal laser scanning microscopy and multispectral cytometry.

    abstract::Numerous highly fluorescent macrophages (MPhi), designated "smoker cells," exist in the lungs of smokers and subjects who have quit smoking within 5 years. The brightly fluorescent MPhi, however, are not present in the lungs of never smokers. Some investigators have speculated that the intense fluorescence of the MPhi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20191

    authors: Pauly JL,Allison EM,Hurley EL,Nwogu CE,Wallace PK,Paszkiewicz GM

    更新日期:2005-06-01 00:00:00

  • Stress and strain distribution in demineralized enamel: A micro-CT based finite element study.

    abstract::Physiological oral mechanical forces may play a role on the progression of enamel carious lesions to cavitation. Thus, the aim of this study was to describe, by 3D finite element analysis, stress, and strain patterns in sound and carious enamel after a simulated occlusal load. Micro-CT based models were created and me...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22549

    authors: Neves AA,Coutinho E,Alves HD,de Assis JT

    更新日期:2015-10-01 00:00:00

  • Human, rhesus macaque, and feline sequences highly similar to mouse mammary tumor virus sequences.

    abstract::Sequences highly similar (>95%) to the mouse mammary tumor virus (MMTV) env gene have been amplified from human DNA samples, including DNA samples from patients with breast cancer (BC) and persons who did not have BC. The sequences from human DNA were distinct from the MMTV sequences used as controls in these PCR reac...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20233

    authors: Szabo S,Haislip AM,Traina-Dorge V,Costin JM,Crawford BE 2nd,Wilson RB,Garry RF

    更新日期:2005-11-01 00:00:00

  • Localization of neurotrophin-3-like immunoreactivity in the rat cochlear nucleus.

    abstract::Immunohistochemistry as well as immunohistofluorescence were used to investigate the distribution of the neurotrophin-3 (NT3) in the adult rat cochlear nucleus. We found a widespread distribution of NT3 immunolabeled neurons throughout the three divisions of this nucleus. NT3-like immunoreactivity was clearly populati...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19980501)41:3<224::AID-JEM

    authors: Burette A,Belliot G,Albuisson E,Romand R

    更新日期:1998-05-01 00:00:00

  • Multidimensional long-term time-lapse microscopy of in vitro peripheral nerve regeneration.

    abstract::In order to test the effectiveness of a new advanced time-lapse microscopy imaging and image processing and analysis system, and to do quantitative and qualitative temporal analyses of in vitro peripheral nerve regeneration, long-term time-lapse imaging of cultures of mouse dorsal root ganglia (DRGs) was performed. DR...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20075

    authors: Oztürk G,Erdoğan E

    更新日期:2004-06-15 00:00:00

  • Virus present in the reproductive tract of asymptomatic drones of honey bee (Apis mellifera l.), and possible infection of queen during mating.

    abstract::Virus particles and viral inclusions were detected by transmission electron microscopy examination of sections of the seminal vesicles and mucus gland of asymptomatic young drones from colonies of Apis mellifera lightly infested by Varroa mite. In the mucus gland the infection was found in the muscular sheath and epit...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22024

    authors: Da Cruz-Landim C,Roat TC,Fernadez FC

    更新日期:2012-07-01 00:00:00

  • Environmental scanning electron microscope imaging examples related to particle analysis.

    abstract::This work provides examples of some of the imaging capabilities of environmental scanning electron microscopy applied to easily charged samples relevant to particle analysis. Environmental SEM (also referred to as high pressure or low vacuum SEM) can address uncoated samples that are known to be difficult to image. Mo...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070250507

    authors: Wight SA,Zeissler CJ

    更新日期:1993-08-01 00:00:00

  • User-friendly semiautomated assembly of accurate image mosaics in microscopy.

    abstract::We present a semiautomated software solution to the problem of extending the lateral field of view of a classical microscope. The initial requirements are a set of overlapping images, along with their user-provided coarse mosaic. Our solution then refines this initial mosaic in a fully automatic fashion. We rely on a ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20393

    authors: Thévenaz P,Unser M

    更新日期:2007-02-01 00:00:00

  • Antennal sensilla of the pine weevil Pissodes nitidus Roel. (Coleoptera: Curculionidae).

    abstract::The antennal sensilla of the pine weevil (Pissodes nitidus Roel.) were observed with scanning electron microscopy and transmission electron microscopy (TEM). The weevil antenna consists of a long scape, a pedicel, and a flagellum with 10 segments; the last four flagellum segments are fused, forming the antennal club, ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20920

    authors: Yan SC,Meng ZJ,Peng L,Liu D

    更新日期:2011-05-01 00:00:00

  • Cellular and molecular biology of ensheathing cells.

    abstract::Ensheathing cells are the glial cells that envelop olfactory axons as they course from the olfactory epithelium to the bulb. They are derived from the olfactory placode and differ from the typical glia in terms of sharing the phenotypes of both astrocytes and Schwann cells. The aims of this study are to review (1) cel...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.10151

    authors: Chuah MI,West AK

    更新日期:2002-08-01 00:00:00

  • Microscopic analysis of the quality of obturation and physical properties of MTA Fillapex.

    abstract::This study analyzed the quality of obturation and physical properties of MTA Fillapex and AH Plus sealer. A sample of 30 human maxillary central incisors were instrumented with Protaper until a F5 (50/05) file. Both sealers were mixed with Rhodamine-B dye to allow visualization on a confocal laser-scanning microscope ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22432

    authors: Amoroso-Silva PA,Guimarães BM,Marciano MA,Duarte MA,Cavenago BC,Ordinola-Zapata R,Almeida MM,Moraes IG

    更新日期:2014-12-01 00:00:00

  • Cardiac neurones of autonomic ganglia.

    abstract::The properties of the postganglionic sympathetic neurones supplying the heart and arising in the stellate and adjacent paravertebral ganglia of various species are discussed with respect to their location, morphology, synaptic input and membrane characteristics. Results from our laboratory on the morphology of rat ste...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19960901)35:1<69::AID-JEMT

    authors: Wallis D,Watson AH,Mo N

    更新日期:1996-09-01 00:00:00

  • Efficacy of calcium- and fluoride-containing materials for the remineralization of primary teeth with early enamel lesion.

    abstract::The aim of the study was to determine the efficacy of different products containing fluoride, calcium and phosphate for enamel remineralization in eroded primary teeth. A total of 90 sound primary canine teeth were randomly divided into 5 groups of 18 teeth each: 1) control (polished enamel), 2) 5% DuraShield sodium f...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22543

    authors: Memarpour M,Soltanimehr E,Sattarahmady N

    更新日期:2015-09-01 00:00:00

  • Investigations of the pathway of incorporation and function of lamin A in the nuclear lamina.

    abstract::Cell-free extracts of Xenopus eggs were used to investigate how differing lamin sub-types are incorporated into a lamina and how lamina composition influences DNA replication. Purified recombinant human lamin A (HlaminA) was inoculated into egg extracts, which support in vitro nuclear assembly. The route of incorporat...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19990401)45:1<1::AID-JEMT1

    authors: Dyer JA,Lane BE,Hutchison CJ

    更新日期:1999-04-01 00:00:00

  • Phase contrast without phase plates and phase rings--optical solutions for improved imaging of phase structures.

    abstract::Using the optical methods described, phase specimens can be observed with a modified light microscope in enhanced clarity, purified from typical artifacts which are apparent in standard phase contrast illumination. In particular, haloing and shade-off are absent, lateral and vertical resolution are maximized and the i...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22265

    authors: Piper T,Piper J

    更新日期:2013-10-01 00:00:00

  • Microstructural morphology of dermal and oral denticles of the sharpnose sevengill shark Heptranchias perlo (Elasmobranchii: Hexanchidae), a deep-water species.

    abstract::The dermal denticles are among the unique morphological adaptations of sharks, which have been acquired throughout their long evolutionary process of more than 400 million years. Species-specific morphological characteristics of these structures has been applied specially as tools for functional and taxonomic (family-...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23273

    authors: Rangel BS,Amorim AF,Kfoury JR Jr,Rici REG

    更新日期:2019-08-01 00:00:00

  • Intramandibular glands in different castes of leaf-cutting ant, Atta laevigata (Fr. Smith, 1858) (Formicidae: Attini).

    abstract::Intramandibular glands have been poorly studied in polymorphic ants, where the differences between castes were unsufficiently scrutinized. Leaf-cutting ants possess one of the most complex systems of communication and labor division, which is polymorphic well as age polyethism, and makes them an ideal model for the st...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22516

    authors: Martins LC,Della Lucia TM,Gonçalves WG,Delabie JH,Zanuncio JC,Serrão JE

    更新日期:2015-07-01 00:00:00