Abstract:
:This ultrastructural study showed that nonisotopic methanolic hafnium chloride and aqueous lead solution was an excellent new electron stain for enhancing TEM contrasts of fungal and plant cell structures. The ultrastructural definition provided by the new stain was often superior to that provided by conventional staining with uranyl acetate and lead. Definition of fine ultrastructure was also supported by quantitative data on TEM contrast ratios of organelles and components in fungal and plant cells. In particular, polysaccharides, which were localized in cell walls, glycogen particles, starch grains, and plant Golgi vesicle components, were much more reactive to the new stain than to the conventional one. The new nonisotopic stain is useful for enhancing the contrast of ultrastructure in biological tissues and is a safer alternative to uranyl acetate.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Ikeda K,Inoue K,Kanematsu S,Horiuchi Y,Park Pdoi
10.1002/jemt.20964subject
Has Abstractpub_date
2011-09-01 00:00:00pages
825-30issue
9eissn
1059-910Xissn
1097-0029journal_volume
74pub_type
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