Abstract:
:Cytochrome c oxidase is a complex integral membrane protein consisting of 13 different polypeptide chains and four metal centers having a total molecular weight of approximately 200,000 daltons. It can be isolated in two 2-dimensional crystalline forms differing in aggregation state of the enzyme. One crystal form consists of cytochrome oxidase dimers (approximately 400,000 daltons) embedded unidirectionally in the lipid bilayer of a collapsed vesicle while the other form consists of crystalline sheets of cytochrome oxidase monomers. Both crystal forms have been studied by electron microscopy during the past two decades, and this paper summarizes the results of early structural studies as well as more recent results applying techniques of cryoelectron microscopy and digital image processing. The structure of frozen-hydrated cytochrome oxidase dimers at 20 A resolution is discussed as well as the packing of monomers within dimers and the site of cytochrome c binding.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Frey TGdoi
10.1002/jemt.1070270407subject
Has Abstractpub_date
1994-03-01 00:00:00pages
319-32issue
4eissn
1059-910Xissn
1097-0029journal_volume
27pub_type
杂志文章,评审abstract::The mammalian gallbladder mucosa is lined by a simple columnar epithelium. Typical surface epithelial cells (principal cells) contain short microvilli, secretory granules, dense bodies, mitochondria and Golgi apparatus. Dense bodies are thought to be lysosomes. Secretory granules contain mucous glycoproteins which are...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970915)38:6<616::AID-JEM
更新日期:1997-09-15 00:00:00
abstract::This study investigated the relationship between enteroendocrine and mucus-secreting cells distribution, the severity of colonic mucosal injury and intestinal motility in experimental colorectal carcinogenesis. Using a standardized murine model of colorectal carcinogenesis, eight-weeks-old female Wistar rats weighting...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22534
更新日期:2016-01-01 00:00:00
abstract::Investigation of mortuary ritual is an important method to reconstruct many aspects of past societies. Due to the lack of relevant analytical work, little evidence related to organic materials in a burial can be found in China. Here we report materials collected from a burial during the excavation of the Shengedaliang...
journal_title:Microscopy research and technique
pub_type: 历史文章,杂志文章
doi:10.1002/jemt.22791
更新日期:2017-02-01 00:00:00
abstract::The glycoconjugate pattern of developing ovarian follicles in wild and cultured Senegalese sole Solea senegalensis was investigated by means of lectin histochemistry. Ovaries from cultured fish contained oocytes up to the late vitellogenic stage, whereas they reached the hydration stage in wild specimens. The follicul...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22040
更新日期:2012-08-01 00:00:00
abstract::Vesicles or caveolae within endothelial cells, fusing together to form vacuolar organelles, are implicated in macromolecular transport and cellular element transmigration across the blood-brain barrier (BBB) during inflammation and ischemia. Vacuolar organelles have been described by transmission electron microscopy a...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20766
更新日期:2010-02-01 00:00:00
abstract::A variety of intracellular recording and staining techniques has been used to establish structure-function and, in some cases, structure-function-neurochemical correlations in fish, turtle, and cat retinae. Cone photoreceptor-horizontal cell connectivity has been studied extensively in the cyprinid fish retina by intr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070240106
更新日期:1993-01-01 00:00:00
abstract::An overview of the morphological correlates of mammalian epidermal lipids and the cutaneous permeability barrier is provided. The following features are discussed: 1) ultrastructural characterization of the progressive lipid alterations accompanying normal stratum corneum (SC) formation; 2) effects of drugs/inhibitors...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970501)37:3<180::AID-JEM
更新日期:1997-05-01 00:00:00
abstract::Nonlinear microscopy techniques are being increasingly used to perform in vivo studies in dermatology. These methods enable us to investigate the morphology and monitor the physiological process in the skin by the use of femtosecond lasers operating in the red, near-infrared spectral range (680-1,300 nm). In this work...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22545
更新日期:2015-09-01 00:00:00
abstract::Gymnosporia arenicola Jordaan (Celastraceae) is a shrub or small tree, which naturally occurs in coastal sand dunes of Southern Mozambique and South Africa. Its dried leaf is often used in traditional medicine for the treatment of infectious and inflammatory diseases. Hereby, we present results of studies carried out ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22565
更新日期:2015-11-01 00:00:00
abstract::The cut gene of Drosophila melanogaster is an identity selector gene that establishes the program of development and differentiation of external sense organs. Mutations in the cut gene cause a transformation of the external sense organs into chordotonal organs, originally assessed by the use of immunostaining methods ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19971215)39:6<492::AID-JEM
更新日期:1997-12-15 00:00:00
abstract::We measured cell membrane deformations under the modulation of piconewton magnetic force by using optical tracking and noninterferometric widefield optical profilometry. The magnetic force was applied to fibronectin-coated paramagnetic beads that bound to transmembrane protein integrins. At an image-acquisition rate o...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20593
更新日期:2008-08-01 00:00:00
abstract::High speed data registration is required for the study of fluorescence resonance energy transfer in real time as well as fast dynamic intra- and inter-cellular signaling events. Multispectral confocal spinning disk microscopy provides a high resolution method for performing such real time live cell imaging. However, o...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20423
更新日期:2007-04-01 00:00:00
abstract::This study evaluated the effect of chitosan, zirconium oxide, and hydroxyapatite on the apatite-like forming ability, porosity, and bond-strength of calcium-aluminate cements (C). Three hundred bovine root-slices were assigned to one of five groups, according to the material: MTA, C, C + chitosan (Cchi), C + zirconium...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23678
更新日期:2020-12-22 00:00:00
abstract::A new and improved technique for microvascular corrosion casting was developed and verified by examination of corrosion casts of 90 bovine limbs. The described technique renders a complete filling of the vasculature of the claw even in regions that hitherto proved to be difficult regarding completeness of filling such...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990501)45:3<184::AID-JEM
更新日期:1999-05-01 00:00:00
abstract::The mint beetle or green mint beetle, Chrysolina herbacea, is one of the most important insect pests of mint herb. These insects are associated with plants belonging to the Lamiaceae family, in particular the Mentha genus. They cause serious damages to plants by consuming their leaves. For this reason, it is an import...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23671
更新日期:2020-12-11 00:00:00
abstract::This study was carried out to identify 20 high valued medicinal plants of District Lahore, Punjab, Pakistan with the help of the palynological studies. The distinguishing characteristics for selected species were found by systematized methods using light microscopy and Scanning Electron Microscopy. In this study, impo...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23096
更新日期:2018-12-01 00:00:00
abstract::Several studies searching for methods to control Rhipicephalus sanguineus s.l., (dog tick) infestations have been developed aiming to minimize the damages caused by these ectoparasites to the hosts and the environment, which is harmed by the indiscriminate use of toxic acaricide products. In this scenario, neem oil ha...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22871
更新日期:2017-08-01 00:00:00
abstract::The subcommissural organ (SCO) is a brain circumventricular organ formed by ependymal and hypendymal secretory cells. It secretes glycoproteins into the cerebrospinal fluid of the third ventricle where they condense into a thread-like structure known as Reissner's fiber (RF). The present study was designed to investig...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/1097-0029(20010301)52:5<496::AID-JEMT1035>
更新日期:2001-03-01 00:00:00
abstract::Femtosecond electron diffraction is a rapidly advancing technique that holds a great promise for studying ultrafast structural dynamics in phase transitions, chemical reactions, and function of biological molecules at the atomic time and length scales. In this paper, we summarize our development of a tabletop femtosec...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20666
更新日期:2009-03-01 00:00:00
abstract::The morphology of pancreatic excretory duct segments was reviewed in mammals. The fine structure of the epithelial lining was described in intercalated ducts, intra- and extralobular ducts, and in major pancreatic ducts. Morphological characteristics of the various cell types comprising to the duct epithelium were det...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970601)37:5/6<407::AID-J
更新日期:1997-06-01 00:00:00
abstract::Lignocellulosic plant cell wall is considered a potential source for second generation biofuels. The plant cell wall is a highly complex structure mainly composed of cellulose, hemicelluloses, and lignin that form a network of crosslinked fibers. The structural organization of the sugarcane cell wall has not been prev...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22235
更新日期:2013-08-01 00:00:00
abstract::Immunohistochemistry as well as immunohistofluorescence were used to investigate the distribution of the neurotrophin-3 (NT3) in the adult rat cochlear nucleus. We found a widespread distribution of NT3 immunolabeled neurons throughout the three divisions of this nucleus. NT3-like immunoreactivity was clearly populati...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980501)41:3<224::AID-JEM
更新日期:1998-05-01 00:00:00
abstract::This report describes osteoclastic bone resorption around intraosseous fixation screws in rat and pig mandibles. These screws supported distraction devices and provided for neutral fixation following the distraction period. Progressive clinical instability of bicortical screws and radiographic and histologic evidence ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10376
更新日期:2003-08-15 00:00:00
abstract::Tumor necrosis factor (TNF) exists in two bioactive forms, the membrane integrated form and the proteolytically derived soluble cytokine. Both forms of TNF are involved in a variety of different physiological and pathophysiological situations. Here we analyzed different human and mouse TNF-specific reagents for their ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/1097-0029(20000801)50:3<243::AID-JEMT8>3.0
更新日期:2000-08-01 00:00:00
abstract::Actin, though highly conserved, exhibits a myriad of diverse functions, most of which ultimately depend on its intrinsic ability to rapidly assemble and disassemble filamentous structures. Many organisms synthesize multiple actin isoforms even within the same cell. Tissue-specific expression patterns and tight develop...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19991001)47:1<38::AID-JEMT
更新日期:1999-10-01 00:00:00
abstract::The antennal flagellum of female Manduca sexta bears eight sensillum types: two trichoid, two basiconic, one auriculate, two coeloconic, and one styliform complex sensilla. The first type of trichoid sensillum averages 34 microm in length and is innervated by two sensory cells. The second type averages 26 microm in le...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1180
更新日期:2001-12-01 00:00:00
abstract::Currently, composite materials dominate among the restorative materials used for direct aesthetic filling. Reinforcement of composites with glass fibers allows for the transfer of greater loads and better durability between the tooth tissue and the filling polymer. New approach to bonding liquid materials with composi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23700
更新日期:2021-01-16 00:00:00
abstract::Assessment of HER2/neu oncogene has been used as both a prognostic and predictive marker for breast cancer. However, the choice of the best method to assess the status of HER2/neu oncogene in breast cancer tissue remains controversial. A variety of techniques are available to detect HER2/neu gene amplification and ove...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10181
更新日期:2002-10-15 00:00:00
abstract::Physiological oral mechanical forces may play a role on the progression of enamel carious lesions to cavitation. Thus, the aim of this study was to describe, by 3D finite element analysis, stress, and strain patterns in sound and carious enamel after a simulated occlusal load. Micro-CT based models were created and me...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22549
更新日期:2015-10-01 00:00:00
abstract::This in vitro study aimed to investigate the tubular penetration depth of four different sealers used with two different obturation methods in the apical, middle, and coronal root canal dentin. Eighty-four single-rooted teeth were instrumented until the F4 ProTaper universal rotary file and filled with AH26, AH Plus (...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23548
更新日期:2020-12-01 00:00:00