Abstract:
:Tumor necrosis factor (TNF) exists in two bioactive forms, the membrane integrated form and the proteolytically derived soluble cytokine. Both forms of TNF are involved in a variety of different physiological and pathophysiological situations. Here we analyzed different human and mouse TNF-specific reagents for their ability to determine the expression of membrane-expressed TNF. The data prove some antibodies to be very useful for the analysis of transmembrane TNF expression because these antibodies distinguish between the transmembrane form of TNF and soluble TNF bound to cellular TNF receptors. In addition, we found that recombinant human TNF receptor fusion proteins are advantageous tools to analyze both human and mouse transmembrane TNF expression.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Gerspach J,Götz A,Zimmermann G,Kolle C,Böttinger H,Grell Mdoi
10.1002/1097-0029(20000801)50:3<243::AID-JEMT8>3.0keywords:
subject
Has Abstractpub_date
2000-08-01 00:00:00pages
243-50issue
3eissn
1059-910Xissn
1097-0029pii
10.1002/1097-0029(20000801)50:3<243::AID-JEMT8>3.0journal_volume
50pub_type
杂志文章abstract::The preparation of optically clear, thick sections of fragile embryonic tissues greatly aids the power of confocal scanning laser microscopy in imaging three-dimensional structures. We report here conditions for embedding, sectioning, and staining embryos in polyacrylamide gels for a variety of confocal imaging techni...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070300608
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abstract::Various types of mirror lenses were developed some decades ago designed for several special tasks in microscopy. When compared with high-end glass lenses, mirror lenses lead to an extraordinary image quality because of their supraapochromatic color fidelity and their high planarity; the working distances are significa...
journal_title:Microscopy research and technique
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abstract::The mechanical properties of healing ligaments and tendons are not comparable to those of normal tissue. To improve the quality of the ligament healing, therapeutic strategies include gene transfer or placement of mesenchymal stem cells at the healing site. Studies show that marker genes, growth factors, and antisense...
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abstract::This article presents an integrated approach for the authentication of five Russian icons through the study of their paint materials (elemental composition and stratigraphic mapping), state of conservation and artistic technique. The results of the applied analytical protocol, involving optical and scanning electron m...
journal_title:Microscopy research and technique
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abstract::We summarize the molecular phenotype, diagnostic criteria, and the newest advances related to seeking the pathogenic mechanism(s) of sporadic inclusion-body myositis (s-IBM), a muscle disease usually of persons over age 50. On the basis of our research, several processes seem to be important in relation to the still-s...
journal_title:Microscopy research and technique
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doi:10.1002/jemt.20186
更新日期:2005-07-01 00:00:00
abstract::This article reviews recent research using a low-energy electron microscope, built by Tromp at IBM, and equipped with an accelerator that permits in situ irradiation with a beam of self-ions. The available ion energies of 20 eV to 5 keV span the range from epitaxial growth by a hyperthermal beam to sputtering at the l...
journal_title:Microscopy research and technique
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doi:10.1002/jemt.20672
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abstract::Ensheathing cells are the glial cells that envelop olfactory axons as they course from the olfactory epithelium to the bulb. They are derived from the olfactory placode and differ from the typical glia in terms of sharing the phenotypes of both astrocytes and Schwann cells. The aims of this study are to review (1) cel...
journal_title:Microscopy research and technique
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abstract::Epidemiologic studies have associated cardiovascular morbidity and mortality with ambient particulate air pollution. Particles smaller than 100 nm in diameter (ultrafine particles) are present in the urban atmosphere in very high numbers yet at very low mass concentration. Organs beyond the lungs are considered as tar...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20044
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abstract::In the present work, we propose the use of the Laser Scanning Confocal Microscopy (LSCM) to determine the effect of water repellents on rock's pore-network configuration and interconnection. The rocks studied are sandstones of Miocene age, a building material that is commonly found in the architectural heritage of Tun...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20624
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abstract::The avian pancreas has three or four lobes and develops from a dorsal and two ventral buds. The cells that will contribute to formation of the dorsal bud are at first located in the mid-dorsal endoderm, those of the ventral buds in the floor of the foregut. The determination of endoderm to form dorsal and ventral bud ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19981115)43:4<292::AID-JEM
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abstract::Several studies support the role of Western-style diet (WD) in inflammatory bowel disease (IBD). Toll-like receptors/NOD-like receptors (TLRs/NLRs) are important to maintain a healthy epithelium as well as inducing inflammation. Given that dietary factors influence IBD development, that epithelial dysfunction is thoug...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22831
更新日期:2017-06-01 00:00:00
abstract::This study evaluated the effect of chitosan, zirconium oxide, and hydroxyapatite on the apatite-like forming ability, porosity, and bond-strength of calcium-aluminate cements (C). Three hundred bovine root-slices were assigned to one of five groups, according to the material: MTA, C, C + chitosan (Cchi), C + zirconium...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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abstract::A photochemical reaction was used to produce focal microcirculation disorders in the guinea pig cochlea. Temporal bones were removed at various intervals between 5 minutes and 1 month after infusion of rose bengal (RB) and illumination. Infused but unilluminated contralateral cochleae served as controls. Dissected coc...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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更新日期:1998-05-15 00:00:00
abstract::Transmission electron microscopy (TEM) is a very useful and commonly used microscopy technique, used especially for the characterization of nanoparticles. However, the identification of the magnetic nanoparticle could be thought problematic in TEM analysis, due to the fact that the magnetic nanoparticles are usually f...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22792
更新日期:2017-02-01 00:00:00
abstract::In spite of accumulating evidence for a modulation of sympathetic neurotransmission by endogenously produced nitric oxide (NO), it remains unclear in which parts of the vascular system and at what level this interaction takes place. The aim of the present study was to investigate the distribution of endothelial and ne...
journal_title:Microscopy research and technique
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doi:10.1002/jemt.1070290214
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journal_title:Microscopy research and technique
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doi:10.1002/jemt.22448
更新日期:2015-01-01 00:00:00
abstract::In mammals, the neurohypophysial hormone oxytocin (OT) is released into the bloodstream during labor and lactation to promote uterine contraction and milk ejection, respectively. Electrophysiological studies have established that OT neurons fire in brief, synchronized bursts during this release. During pregnancy and l...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10019
更新日期:2002-01-15 00:00:00
abstract::This study was performed to observe microstructures of the rat lung, using a synchrotron radiation beam and to compare findings with histological observations. X-ray refraction images from ex-vivo ventilating rat lung were obtained with an 8 KeV monochromatic beam and 20-mum thick CsI(Tl) scintillation crystal. The vi...
journal_title:Microscopy research and technique
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doi:10.1002/jemt.20335
更新日期:2006-08-01 00:00:00
abstract::Sequences highly similar (>95%) to the mouse mammary tumor virus (MMTV) env gene have been amplified from human DNA samples, including DNA samples from patients with breast cancer (BC) and persons who did not have BC. The sequences from human DNA were distinct from the MMTV sequences used as controls in these PCR reac...
journal_title:Microscopy research and technique
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更新日期:2005-11-01 00:00:00
abstract::Transfer of a fluorescently labeled protein particle from a surface to a microsized scanning probe has been induced by repetitive scanning in aqueous medium. The so-attached particle can in turn act as a probing tool to study particle-substrate and particle-particle interactions. Attachment of the fluorescent particle...
journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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abstract::A common feature in the configuration of germinal vesicle (GV) chromatin in most species is that diffuse chromatin condenses into a perinucleolar ring during follicular growth; however, no such ring was observed in goat oocytes. Reports on whether bovine GV chromatin condenses into a perinucleolar ring are controversi...
journal_title:Microscopy research and technique
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doi:10.1002/jemt.20349
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abstract::A homemade colloidal probe atomic force microscope was used to perform nanoindentation with a spherical probe of 5 microm in diameter, at different approach velocities in order to extract the Young's modulus, E0, of poly(L-lysine)/hyaluronan (PLL/HA) films. This parameter is of prime importance to control cellular adh...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20275
更新日期:2006-02-01 00:00:00
abstract::Anatomical study of leaf xylary vessel elements of Carthamus oxycantha under various intensities of lead (Pb) and nickel (Ni) stress (200, 400, 600, and 800 mg Pb(NO3 )2 , NiCl2 ·6H2 O/kg of the soil) was conducted. The deformations caused due to metal stress were detected using point-based image registration techniqu...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23476
更新日期:2020-07-01 00:00:00
abstract::Different methods have been established for the simultaneous detection of different pathogens in tissue samples, each with certain advantages and disadvantages. Chromogenic in situ hybridization combines specific molecular pathogen detection with microscopic evaluation of pathogen quantity, morphology and distribution...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20546
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abstract::Many aspects of the organization of the electromotor synapse of electric fish resemble the nerve-muscle junction. In particular, the postsynaptic membrane in both systems share most of their proteins. As a remarquable source of cholinergic synapses, the Torpedo electrocyte model has served to identify the most importa...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(20000401)49:1<73::AID-JEMT
更新日期:2000-04-01 00:00:00
abstract::Laser scanning confocal microscopy (LSCM) and atomic force microscopy (AFM) were used to characterize changes in nanoscale structure that occur when ultrathin polyelectrolyte multilayers (PEMs) are incubated in aqueous media. The PEMs investigated here were fabricated by the deposition of alternating layers of plasmid...
journal_title:Microscopy research and technique
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doi:10.1002/jemt.20830
更新日期:2010-09-01 00:00:00
abstract::In the current investigation, 16 samples of six species of the genus Polygonatum were collected from different geographical regions in China. Leaf micromorphological traits were studied in detail using scanning electron microscopy. About five-leaf samples were used for each species collected from different geographica...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23529
更新日期:2020-11-01 00:00:00
abstract::In certain cases of infertility in domestic species, and in the homozygous c-ros tyrosine kinase knockout mouse, males are infertile as a result of unopposed sperm swelling. This induces flagellar angulation, preventing their normal migration in the female tract. Angulation always occurs at the site of the cytoplasmic...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10314
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abstract::Cell-free extracts of Xenopus eggs were used to investigate how differing lamin sub-types are incorporated into a lamina and how lamina composition influences DNA replication. Purified recombinant human lamin A (HlaminA) was inoculated into egg extracts, which support in vitro nuclear assembly. The route of incorporat...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990401)45:1<1::AID-JEMT1
更新日期:1999-04-01 00:00:00