Abstract:
:Assessment of HER2/neu oncogene has been used as both a prognostic and predictive marker for breast cancer. However, the choice of the best method to assess the status of HER2/neu oncogene in breast cancer tissue remains controversial. A variety of techniques are available to detect HER2/neu gene amplification and overexpression. Tissue-based detection methods by immunohistochemistry (IHC) and/or fluorescence in situ hybridization (FISH) offers a clear advantage over other approaches. FISH is a costly and relatively difficult assay and yet appears to be a better predictor of response to Herceptin (Trastuzumab) therapy and patient outcome. IHC is less expensive and is easier to perform; however, it suffers from a high rate of false negativity and positivity as well as inter-observer variability among pathologists. Suggestions have been made to use IHC as a screening procedure followed by confirmation by FISH in selected cases. Considering the importance of an accurate assessment of HER2/neu oncogene in selecting therapy, a better alternative may be to use FISH as the primary testing for HER2/neu oncogene. Herceptin therapy is associated with several side effects and is expensive. Thus, in the long term, it may be more cost-effective to use the FISH procedure and reduce the possibility of under-treatment or over-treatment of breast cancer patients. In addition, assessment of HER2/neu oncogene on every newly diagnosed early breast carcinoma may not be necessary. Metastatic lesions, when they occur, can be sampled by fine needle aspiration biopsy or core needle biopsy for assessment of HER2/Neu status.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Masood S,Bui MMdoi
10.1002/jemt.10181keywords:
subject
Has Abstractpub_date
2002-10-15 00:00:00pages
102-8issue
2eissn
1059-910Xissn
1097-0029journal_volume
59pub_type
杂志文章,评审abstract::Golgi impregnations reveal a variety of dendritic morphologies amongst Kenyon cells in the mushroom bodies of Drosophila melanogaster. Different morphological types of Kenyon cells contribute axon-like processes to five divisions of the medial and vertical lobes. Four of these divisions have characteristic affinities ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10368
更新日期:2003-10-01 00:00:00
abstract::During the past few years, cyto- and immunocytochemical techniques have been developed and widely used for locating and identifying various molecules in plant cell compartments. The last decade has witnessed tremendous improvements in molecular cytology, thus allowing an accurate in situ detection of various component...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070310106
更新日期:1995-05-01 00:00:00
abstract::This paper describes the use of a photobleach method and confocal microscopy to compare the cell-to-cell transfer rate of 5,6 carboxyfluorescein in dissociated embryonic chick lens cells with those in the anterior epithelium of the whole embryonic chick lens. The average cell-to-cell transfer rates obtained were 7.9 x...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070310508
更新日期:1995-08-01 00:00:00
abstract::Studies on embryonic development in vitro as well as observations in vivo, suggested that two aspects of oviduct physiology are important for early development. On one side has to be considered the oviduct "environment": temperature, pH, osmotic pressure, nutrients, oxygen tension, free radical scavengers, etc. On the...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070320102
更新日期:1995-09-01 00:00:00
abstract::Insects rely heavily on olfaction to locate habitat, mates, and oviposition sites, while odorant molecules and the antennal olfactory sensory cells of insects are two indispensable components of olfactory response. Our previous work identified the sex pheromones and volatile compounds derived from host plant of Dendro...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22134
更新日期:2013-01-01 00:00:00
abstract::In this paper we present the state of knowledge on cell-cell interactions in the testis of two groups of anamniote vertebrates--teleosts and elasmobranchs--which include most fish. In these fish, the structural organization of the testis differs fundamentally from that which characterizes amniotes in which the germina...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070320606
更新日期:1995-12-15 00:00:00
abstract::Nonlinear microscopy techniques are being increasingly used to perform in vivo studies in dermatology. These methods enable us to investigate the morphology and monitor the physiological process in the skin by the use of femtosecond lasers operating in the red, near-infrared spectral range (680-1,300 nm). In this work...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22545
更新日期:2015-09-01 00:00:00
abstract::The Neotropical catfish, Corydoras paleatus (Callichthyidae) is a facultative air-breathing teleost that makes use of the caudal portion of the intestine as an accessory air-breathing organ. This portion is highly modified, being well vascularized with capillaries between epithelial cells, which makes it well suited f...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22614
更新日期:2016-03-01 00:00:00
abstract::The possibility that tannic acid and other classical mordants can be used in the electron microscopical section staining technique has been tested. A mordant can be defined as a chemical that combines with both a certain specific tissue component and the staining substance and thereby permits a staining reaction that ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070210110
更新日期:1992-03-01 00:00:00
abstract::Among the five senses, the sense of smell (olfaction) is the most sensitive and emotional window on the outside world (Stern and Marx, 1999). The olfactory system recognizes and discriminates myriad odorants of diverse molecular structures. What makes the olfactory system so specific and sensitive? OE harboring the ol...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10130
更新日期:2002-08-01 00:00:00
abstract::Nowadays the processes of crossing of different mammalian species are well characterized in light of genetics, and possibility of reproduction. Generally, lack detailed studies on changes in anatomy and histology of internal systems of hybrids. Bison bonasus hybrid is an interspecific species between the bull of Europ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23516
更新日期:2020-10-01 00:00:00
abstract::The effectiveness of different microscopy techniques for measuring the dimensions of ultimate fibers from harakeke (Phormium tenax, New Zealand flax) was investigated using a factorial experimental design. Constant variables were geographical location, location of specimens along the leaf, season (winter), individual ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20206
更新日期:2005-08-01 00:00:00
abstract::Choroid plexus (CP) is an important target organ for polypeptides. The fenestrated phenotype of choroidal endothelium facilitates the penetration of blood-borne polypeptides across the capillary walls. Thus, both circulating and cerebrospinal fluid (CSF)-borne polypeptides can reach their receptors on choroidal epithe...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20010101)52:1<65::AID-JEMT9>3.0.
更新日期:2001-01-01 00:00:00
abstract::The implantation of breast prostheses for both aesthetic and reconstructive purposes has been growing exponentially in the last 20 years. Safety and prosthesis lifespan are majorly debated issues in relation to the correlated long-term complications. Mainly the periprosthetic capsule that develops around the implant i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23673
更新日期:2020-12-10 00:00:00
abstract::Effects of microstructure and phase component on mechanical property of spearer propodus of mantis shrimp were investigated. The spearer propodus consisted of three layers including epicuticle (outer layer), exocuticle (middle layer), and endocuticle (inner layer). The outer layer was composed of fluorapatite, which w...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23598
更新日期:2020-09-16 00:00:00
abstract::This review article focuses on the ontogeny and the regulatory mechanisms involved in the modulation of the intracellular events governing the assembly and delivery of lipoproteins in human gut. The human fetal intestine organizes villi covered with well-differentiated enterocytes during the end of the first trimester...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(20000515)49:4<363::AID-JEM
更新日期:2000-05-15 00:00:00
abstract::The present paper covers the results of different transmission electron microscopy studies on silver halide microcrystals. Pure AgBr as well as core-shell AgBr-AgBrI crystals are investigated. In the former parallel and non-parallel twinning modes yielding tabular and needle- or tetrahedral-shaped microcrystals, respe...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980715)42:2<85::AID-JEMT
更新日期:1998-07-15 00:00:00
abstract::We combine reflective confocal microscopy with multiphoton microscopy to form a minimally invasive technique to observe the cornea. The two imaging modalities allow detection of complementary information from the cornea. The autofluorescence signal shows the cytoplasm of epithelial cells, and the second harmonic gener...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20528
更新日期:2008-02-01 00:00:00
abstract::Cytochrome c oxidase is a complex integral membrane protein consisting of 13 different polypeptide chains and four metal centers having a total molecular weight of approximately 200,000 daltons. It can be isolated in two 2-dimensional crystalline forms differing in aggregation state of the enzyme. One crystal form con...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070270407
更新日期:1994-03-01 00:00:00
abstract::Oligodendrocytes (OL) play a significant physiological role in the central nervous system by creating the myelin sheath that allows for the efficient conduction of nerve impulses. Therefore, it is important to understand which signalling cascades define the proliferation, differentiation, survival, and myelin formatio...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1052
更新日期:2001-03-15 00:00:00
abstract::Magnetotactic bacteria orient and migrate along geomagnetic field lines. Each cell contains membrane-enclosed, nano-scale, iron-mineral particles called magnetosomes that cause alignment of the cell in the geomagnetic field as the bacteria swim propelled by flagella. In this work we studied the ultrastructure of the f...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20380
更新日期:2007-01-01 00:00:00
abstract::Numerous highly fluorescent macrophages (MPhi), designated "smoker cells," exist in the lungs of smokers and subjects who have quit smoking within 5 years. The brightly fluorescent MPhi, however, are not present in the lungs of never smokers. Some investigators have speculated that the intense fluorescence of the MPhi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20191
更新日期:2005-06-01 00:00:00
abstract::Term and preterm cervical ripening and dilatation have similarities with an inflammatory reaction. Since cell adhesion molecules are involved in this process, investigations on the expression of intercellular adhesion molecule-1, endothelial leukocyte adhesion molecule-1, vascular cell adhesion molecule-1, and platele...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10281
更新日期:2003-03-01 00:00:00
abstract::Macro and micromorphology of different parts that is, sepals, petals, flowers, indumentum, and leaf shape of 22 Geranium L. taxa from Iran were studied. These taxa representing the eight sections of the genus as sect. Dissecta, sect. Geranium, and sect. Tuberosa (of subgen. Geranium); sect. Batrachioidea, sect. Divari...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23159
更新日期:2018-12-01 00:00:00
abstract::The ultrastructural characteristics of peptidergic peripheral contacts in the snail, Helix pomatia, were investigated, with special attention to the innervation of the heart, buccal mass, and salivary gland by Mytilus inhibitory peptide-immunoreactive neurons. Following the application of correlative light- and electr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/1097-0029(20000615)49:6<534::AID-JEMT4>3.0
更新日期:2000-06-15 00:00:00
abstract::Orthodontic appliances causes specific alterations in oral environment, including reduction of pH, increase of dental biofilm and elevation of salivary microbial levels, causing an increased risk for dental caries. This study evaluated, using microbial culture and scanning electron microscopy (SEM), the in situ contam...
journal_title:Microscopy research and technique
pub_type: 杂志文章,随机对照试验
doi:10.1002/jemt.22817
更新日期:2017-05-01 00:00:00
abstract::Uranium-contaminated soils from the U.S. Department of Energy (DOE) Fernald Site, Ohio, have been examined by a combination of backscattered electron imaging (BSE) and analytical electron microscopy with electron diffraction (AEM). The inhomogeneous distribution of particulate uranium phases in the soil required the d...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070310208
更新日期:1995-06-01 00:00:00
abstract::This study was carried out to identify 20 high valued medicinal plants of District Lahore, Punjab, Pakistan with the help of the palynological studies. The distinguishing characteristics for selected species were found by systematized methods using light microscopy and Scanning Electron Microscopy. In this study, impo...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23096
更新日期:2018-12-01 00:00:00
abstract::Surface ultrastructures of gill filaments and secondary lamellae of Rita rita and Cirrhinus mrigala, inhabiting different ecological habitat, were investigated to unravel adaptive modifications. R. rita is a sluggish, bottom dwelling carnivorous catfish, which inhabits regions of river with accumulations of dirty wate...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.21074
更新日期:2012-04-01 00:00:00
abstract::Red myofibers in mouse soleus muscle have two spatially distinct populations of mitochondria: one where these organelles are disposed in large clusters just inside the sarcolemma and the other situated between the myofibrils. In most cases, the interfibrillar mitochondria (IFM), which are much smaller than the subsarc...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22158
更新日期:2013-03-01 00:00:00