Biofilm formation in Haas palatal expanders with and without use of an antimicrobial agent: an in situ study.

Abstract:

:Orthodontic appliances causes specific alterations in oral environment, including reduction of pH, increase of dental biofilm and elevation of salivary microbial levels, causing an increased risk for dental caries. This study evaluated, using microbial culture and scanning electron microscopy (SEM), the in situ contamination by mutans streptococci (MS) of different surfaces of Haas palatal expanders with and without use of chlorhexidine gluconate mouthrinses (CHX). Thirty-four patients were randomly assigned to two groups (n = 17/group), using placebo (Group I) and 0.12% CHX (Group II-Periogard® ) mouthrinses twice a week. After 4 months, appliances were submitted to microbiological processing and after fragments were analyzed by SEM. Mann-Whitney U test (α = 5%) was used to assess differences between groups on the appliances' different surfaces and to compare the contamination on the free and nonfree surfaces of these components. There was no difference (p = 0.999) between groups regarding the number of MS colonies/biofilms on the nonfree surfaces, which showed intense contamination. However, free surfaces of Group II presented less contamination (p < 0.001) than those of Group I in all appliances' components. Results of the microbial culture were confirmed by SEM. Use of 0.12% CHX was effective in reducing the formation of MS colonies/biofilms on free surfaces of Haas expanders, in situ.

journal_name

Microsc Res Tech

authors

Bagatin CR,Andrucioli MCD,Ferreira JTL,Matsumoto MAN,da Silva RAB,da Silva LAB,Romano FL,Nelson-Filho P

doi

10.1002/jemt.22817

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

471-477

issue

5

eissn

1059-910X

issn

1097-0029

journal_volume

80

pub_type

杂志文章,随机对照试验
  • Immunocytochemistry of plant defense mechanisms induced upon microbial attack.

    abstract::During the past few years, cyto- and immunocytochemical techniques have been developed and widely used for locating and identifying various molecules in plant cell compartments. The last decade has witnessed tremendous improvements in molecular cytology, thus allowing an accurate in situ detection of various component...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1070310106

    authors: Benhamou N

    更新日期:1995-05-01 00:00:00

  • Ultrastructural analysis of murine hippocampal neural progenitor cells in culture.

    abstract::Despite the great number of studies devoted to neural stem/progenitor cell biology, the ultrastructural characteristics of these cells in vitro have not been fully studied. To determine the fine structure of hippocampal neural progenitor cells (NPCs) in culture, mouse fetal hippocampi (E18) were extracted, dissected, ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22454

    authors: Tsupykov O

    更新日期:2015-02-01 00:00:00

  • Thymic microvascular system.

    abstract::Morphological studies of the microcirculatory system in the thymus were reviewed in regards to methodology and structural organization of blood and lymphatic vessels. The blood capillaries and postcapillary venules (PCVs) in the thymus are characterized by a double-walled structure. These vessels are surrounded more o...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19970801)38:3<287::AID-JEM

    authors: Kato S

    更新日期:1997-08-01 00:00:00

  • Antennal sensilla of Apanteles cypris Nixon (Hymenoptera: Braconidae), a larval endoparasitoid of Cnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae).

    abstract::Apanteles cypris is an endoparasitoid of rice pest Cnaphalocrocis medinalis. To better understand its host location mechanism, we examined the external morphology of antennal sensilla of A. cypris via scanning electron microscopy. The antennae of male and female A. cypris were filiform in shape, which consist of scape...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.21016

    authors: Zhou H,Wu WJ,Zhnag ZF,Zhnag Y

    更新日期:2011-12-01 00:00:00

  • High resolution and image processing of otoconia matrix.

    abstract::This study was designed to investigate patterns of fibrils organization in histochemically stained otoconia. Transmission electron microscope and video imaging were used. These data indicate that otoconia of the chick (Gallus domesticus) inner ear may have central cores in vivo. The data also show that the ultrastruct...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070250406

    authors: Fermin CD

    更新日期:1993-07-01 00:00:00

  • Sugarcane cell wall structure and lignin distribution investigated by confocal and electron microscopy.

    abstract::Lignocellulosic plant cell wall is considered a potential source for second generation biofuels. The plant cell wall is a highly complex structure mainly composed of cellulose, hemicelluloses, and lignin that form a network of crosslinked fibers. The structural organization of the sugarcane cell wall has not been prev...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22235

    authors: Sant'Anna C,Costa LT,Abud Y,Biancatto L,Miguens FC,de Souza W

    更新日期:2013-08-01 00:00:00

  • High-resolution X-ray refraction imaging of rat lung and histological correlations.

    abstract::This study was performed to observe microstructures of the rat lung, using a synchrotron radiation beam and to compare findings with histological observations. X-ray refraction images from ex-vivo ventilating rat lung were obtained with an 8 KeV monochromatic beam and 20-mum thick CsI(Tl) scintillation crystal. The vi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20335

    authors: Jheon S,Youn HS,Kim HT,Choi GH,Kim JK

    更新日期:2006-08-01 00:00:00

  • Investigation of mineral content of root canal dentin after the application of various antibiotic paste using energy-dispersive X-ray detector.

    abstract::The purpose of this study was to evaluate mineral content of root canal dentin after treatment with different antibiotic pastes including the mixture of metronidazole, ciprofloxacin, doxycycline, cefaclor, amoxicillin, or minocycline. Fifty extracted maxillary canine teeth were randomly divided into five groups (n = 1...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23155

    authors: Simsek H,Coruh M,Cakici F,Fundaoglu Kucukekenci F,Gurbuz T,Cakici EB

    更新日期:2019-02-01 00:00:00

  • The torpedo electrocyte: a model system to study membrane-cytoskeleton interactions at the postsynaptic membrane.

    abstract::Many aspects of the organization of the electromotor synapse of electric fish resemble the nerve-muscle junction. In particular, the postsynaptic membrane in both systems share most of their proteins. As a remarquable source of cholinergic synapses, the Torpedo electrocyte model has served to identify the most importa...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(20000401)49:1<73::AID-JEMT

    authors: Cartaud J,Cartaud A,Kordeli E,Ludosky MA,Marchand S,Stetzkowski-Marden F

    更新日期:2000-04-01 00:00:00

  • Arrangement and fine structure of collagen fibrils in the decidualized mouse endometrium.

    abstract::The adaptations of the mouse uterus to pregnancy include extensive modifications of the cells and extracellular matrix of the endometrial connective tissue that surround the embryos. Around each implanted embryo this tissue redifferentiates into a transient structure called decidua, which is formed by polygonal cells ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20265

    authors: Carbone K,Pinto NM,Abrahamsohn PA,Zorn TM

    更新日期:2006-01-01 00:00:00

  • Resolving morphology and antibody labeling over large distances in tissue sections.

    abstract::Protein expression patterns are a primary determinant of tissue function and in this study we developed methods to study protein expression over macroscopic distances at subcellular levels of detail. Using the mammalian lens as a model tissue system, we show that by combining two-photon microscopy with novel image mon...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.10360

    authors: Jacobs MD,Donaldson PJ,Cannell MB,Soeller C

    更新日期:2003-09-01 00:00:00

  • Transgenic models of pituitary diseases.

    abstract::Transgenic mice are valuable experimental models of human endocrine diseases. Targeted ablation of specific cell lineages or insertion of genes coding for releasing factors, hormones, growth factors, and oncogenes fused with appropriate promoters, or mutated genes, can induce several pituitary disorders. Various hypos...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19971015)39:2<194::AID-JEM

    authors: Stefaneanu L,Kovacs K

    更新日期:1997-10-15 00:00:00

  • Developmental aspects of lipid and lipoprotein synthesis and secretion in human gut.

    abstract::This review article focuses on the ontogeny and the regulatory mechanisms involved in the modulation of the intracellular events governing the assembly and delivery of lipoproteins in human gut. The human fetal intestine organizes villi covered with well-differentiated enterocytes during the end of the first trimester...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(20000515)49:4<363::AID-JEM

    authors: Levy E,Ménard D

    更新日期:2000-05-15 00:00:00

  • Angiogenesis of buffalo choroid plexuses: structural and immunocytochemical study.

    abstract::Mammalian choroid plexuses (CPs) are vascularized structures involved in numerous exchange processes that supply nutrients and hormones to the brain, and that remove deleterious compounds and metabolites from the brain. Studies in the adult Mediterranean buffalo have investigated the morphology of CPs using histochemi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22037

    authors: Scala G,Maruccio L

    更新日期:2012-08-01 00:00:00

  • Nanoparticle embedded chitosan film for agglomeration free TEM images.

    abstract::Transmission electron microscopy (TEM) is a very useful and commonly used microscopy technique, used especially for the characterization of nanoparticles. However, the identification of the magnetic nanoparticle could be thought problematic in TEM analysis, due to the fact that the magnetic nanoparticles are usually f...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22792

    authors: Dogan Ü,Çiftçi H,Cetin D,Suludere Z,Tamer U

    更新日期:2017-02-01 00:00:00

  • X-ray microanalysis of organic thin sections in TEM using an UTW Si(Li) detector: comparison of quantification methods.

    abstract::We compared Hall's peak to continuum ratio method with a peak ratio method in order to quantify light elements (C, N, and O) in organic specimens as a model for biological thin sections. X-ray spectra were recorded by an energy dispersive X-ray spectrometer equipped with an ultra thin window detector. Spectra were pro...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/1097-0029(20010115)52:2<231::AID-JEMT1008>

    authors: Laquerriere P,Banchet V,Michel J,Zierold K,Balossier G,Bonhomme P

    更新日期:2001-01-15 00:00:00

  • Cell and matrix morpho-functional analysis in chondrocyte micromasses.

    abstract::Micromass cultures represent a convenient means of studying chondrocyte physiology in the context of a tridimensional culture model. In this study, we present the first ultrastructural analysis of the distribution and organization of the extracellular components in micromasses in comparison with their cartilaginous co...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20210

    authors: Battistelli M,Borzì RM,Olivotto E,Vitellozzi R,Burattini S,Facchini A,Falcieri E

    更新日期:2005-08-15 00:00:00

  • Evidence for ciliary pigment localization in colored ciliates and implications for their photosensory transduction chain: a confocal microscopy study.

    abstract::In this study we report for the first time the localization of a photoreceptor pigment in the cilia of the colored heterotrich ciliates Blepharisma japonicum red and blue form, Fabrea salina, and Stentor coeruleus, as result of a confocal microscopy investigation. Optical sectioning confocal microscopy has been used f...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20508

    authors: Colombetti G,Checcucci G,Lucia S,Usai C,Ramoino P,Bianchini P,Pesce M,Vicidomini G,Diaspro A

    更新日期:2007-12-01 00:00:00

  • Palynological investigation of lactiferous flora (Apocynaceae) of District Rawalpindi, Pakistan, using light and scanning electron microscopy.

    abstract::This was the first comprehensive pollen micromorphological investigation of lactiferous flora (Apocynaceae) of District Rawalpindi, Pakistan. The pollen morphology of 10 species of the family Apocynaceae was observed and documented using light microscopy and scanning electron microscopy. Pollen was found subspheroidal...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23293

    authors: Naz S,Zafar M,Ahmad M,Memon RA,Sultana S,Bahadur S,Ozdemir FA,Siddiq Z,Shah MA

    更新日期:2019-09-01 00:00:00

  • Genistein affects ovarian folliculogenesis: a stereological study.

    abstract::The effects of short-term genistein exposure on ovarian folliculogenesis in immature rats were examined stereologically. To determine whether genistein acts as an estrogen agonist or antagonist, the results were compared with the effects of 17α-ethynylestradiol. Immature female rats received 50 mg/kg/bw of genistein i...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22117

    authors: Medigović I,Ristić N,Trifunović S,Manojlović-Stojanoski M,Milošević V,Zikić D,Nestorović N

    更新日期:2012-12-01 00:00:00

  • Pododermal angioarchitecture of the bovine claw in relation to form and function of the papillary body: a scanning electron microscopic study.

    abstract::The region-specific angioarchitecture of the bovine pododerma was examined using systematic scanning electron microscopy of micro-corrosion casts of juvenile and adult bovine claws. Particular emphasis was laid on the demonstration of specialised vascular structures such as arteriovenous anastomoses. Comparing the res...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1150

    authors: Hirschberg RM,Mülling CK,Budras KD

    更新日期:2001-09-15 00:00:00

  • Ultrastructural neurobiology of the olfactory mucosa of the brown trout, Salmo trutta.

    abstract::This paper describes four investigations of the olfactory mucosa of the brown trout: 1) the ultrastructure of the olfactory mucosa as revealed by scanning (SEM), conventional transmission (TEM), and high voltage (HVEM) electron microscopy; 2) light and electron-microscopic investigations of retrograde transport of the...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070230104

    authors: Moran DT,Rowley JC 3rd,Aiken GR,Jafek BW

    更新日期:1992-10-01 00:00:00

  • Biochemical and morphological alterations in the Achilles tendon of mdx mice.

    abstract::Dystrophin-deficient muscles have repeated cycles of necrosis and regeneration, being susceptible to injury induced by muscle contractions. Some studies have demonstrated that tendons are also affected in mdx mice, based especially on the changes in biomechanical properties arising from the respective linked muscles. ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22448

    authors: De Aro AA,Guerra Fda R,Esquisatto MA,Nakagaki WR,Gomes L,Pimentel ER

    更新日期:2015-01-01 00:00:00

  • The mushroom bodies of Drosophila melanogaster: an immunocytological and golgi study of Kenyon cell organization in the calyces and lobes.

    abstract::Golgi impregnations reveal a variety of dendritic morphologies amongst Kenyon cells in the mushroom bodies of Drosophila melanogaster. Different morphological types of Kenyon cells contribute axon-like processes to five divisions of the medial and vertical lobes. Four of these divisions have characteristic affinities ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.10368

    authors: Strausfeld NJ,Sinakevitch I,Vilinsky I

    更新日期:2003-10-01 00:00:00

  • Immunocytochemical localization of adenylyl cyclase in human myometrium.

    abstract::The enzyme adenylyl cyclase (AC) plays a pivotal role in smooth muscle relaxation. Biochemical evidence suggests that AC is predominantly located in the outer layers of the myometrium; however, neither immunocytochemical nor histochemical studies have been undertaken to demonstrate the specific cellular distribution o...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19980301)40:6<488::AID-JEM

    authors: Richards PD,Tiltman AJ,Richards PA

    更新日期:1998-03-15 00:00:00

  • Functions of proteins secreted by oviduct epithelial cells.

    abstract::Studies on embryonic development in vitro as well as observations in vivo, suggested that two aspects of oviduct physiology are important for early development. On one side has to be considered the oviduct "environment": temperature, pH, osmotic pressure, nutrients, oxygen tension, free radical scavengers, etc. On the...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1070320102

    authors: Gandolfi F

    更新日期:1995-09-01 00:00:00

  • Round window membrane. Structure function and permeability: a review.

    abstract::The ultrastructure of the round window membrane of humans, monkeys, felines, and rodents discloses three basic layers: an outer epithelium, a middle core of connective tissue, and an inner epithelium. Interspecies variations are mainly in terms of thickness, being thinnest in rodents and thicker in humans. Morphologic...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19970201)36:3<201::AID-JEM

    authors: Goycoolea MV,Lundman L

    更新日期:1997-02-01 00:00:00

  • Role of Oct-6 in Schwann cell differentiation.

    abstract::Research into the POU transcription factor Oct-6 has been the focus of much current attention, in particular its role in Schwann cell development and differentiation. Based on published data and data presented here, we propose a model for Oct-6 function at two distinct stages of Schwann cell maturation. First, Oct-6 f...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19980601)41:5<372::AID-JEM

    authors: Jaegle M,Meijer D

    更新日期:1998-06-01 00:00:00

  • The microcomputer and image analysis in diagnostic pathology.

    abstract::This paper presents a snapshot view of the influence and direction of microcomputer technology for image analysis techniques in diagnostic pathology. Microcomputers have had considerable impact in bringing image analysis to wider application. Semi-automated tracing techniques are a simple means of providing objective ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1070210406

    authors: Jarvis LR

    更新日期:1992-06-01 00:00:00

  • Application limits and data correction in number of molecules and brightness analysis.

    abstract::Number of molecules and Brightness (N&B) has been proposed for measuring the molecular brightness and number of fluorophores in time-sequence of images, in live cells. If the fluorescently tagged-proteins are mobile in the illumination volume, the stoichiometry of their oligomers can be derived from the increase of th...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22277

    authors: Trullo A,Corti V,Arza E,Caiolfa VR,Zamai M

    更新日期:2013-11-01 00:00:00