High-resolution X-ray refraction imaging of rat lung and histological correlations.

Abstract:

:This study was performed to observe microstructures of the rat lung, using a synchrotron radiation beam and to compare findings with histological observations. X-ray refraction images from ex-vivo ventilating rat lung were obtained with an 8 KeV monochromatic beam and 20-mum thick CsI(Tl) scintillation crystal. The visual image was magnified using a 20x microscope objective and captured using an analog CCD camera. Obtained images were compared with conventional light microscopic findings from the same tissue. Pulmonary microstructures, including alveolar ducts, alveolar sacs, alveoli, alveolar walls, and perialveolar capillary networks were clearly identified with spatial resolution of as much as 1.2 mum and had good correlation with conventional light microscopic findings. The shape of alveoli appeared more round in SR images than in the light microscopic images. The results suggest that X-ray microscopy study of the lung using synchrotron radiation demonstrates the potential for clinically relevant microstructure of lung tissue without sectioning and fixation.

journal_name

Microsc Res Tech

authors

Jheon S,Youn HS,Kim HT,Choi GH,Kim JK

doi

10.1002/jemt.20335

subject

Has Abstract

pub_date

2006-08-01 00:00:00

pages

656-9

issue

8

eissn

1059-910X

issn

1097-0029

journal_volume

69

pub_type

杂志文章
  • Response of donor and recipient cells after transplantation of cells to the ligament and tendon.

    abstract::The mechanical properties of healing ligaments and tendons are not comparable to those of normal tissue. To improve the quality of the ligament healing, therapeutic strategies include gene transfer or placement of mesenchymal stem cells at the healing site. Studies show that marker genes, growth factors, and antisense...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.10114

    authors: Hildebrand KA,Jia F,Woo SL

    更新日期:2002-07-01 00:00:00

  • Dynamic structural changes of synaptic contacts in the visual system of insects.

    abstract::The visual system of insects provides an excellent model to study processes of transduction and transmission of photic information, synaptogenesis, synaptic plasticity, and wiring between photoreceptors and their visual interneurons in the optic lobe. This review describes synaptic contacts between photoreceptors and ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.10141

    authors: Pyza E

    更新日期:2002-08-15 00:00:00

  • Image mean square displacement to study the lateral mobility of Angiotensin II type 1 and Endothelin 1 type A receptors on living cells.

    abstract::The lateral mobility of membrane receptors provides insights into the molecular interactions of protein binding and the complex dynamic plasma membrane. The image mean square displacement (iMSD) analysis is a method used to extract qualitative and quantitative information of the protein diffusion law and infers how di...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23425

    authors: Planes N,Vanderheyden PPML,Gratton E,Caballero-George C

    更新日期:2020-04-01 00:00:00

  • The mushroom bodies of Drosophila melanogaster: an immunocytological and golgi study of Kenyon cell organization in the calyces and lobes.

    abstract::Golgi impregnations reveal a variety of dendritic morphologies amongst Kenyon cells in the mushroom bodies of Drosophila melanogaster. Different morphological types of Kenyon cells contribute axon-like processes to five divisions of the medial and vertical lobes. Four of these divisions have characteristic affinities ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.10368

    authors: Strausfeld NJ,Sinakevitch I,Vilinsky I

    更新日期:2003-10-01 00:00:00

  • Fragile X syndrome, the Fragile X related proteins, and animal models.

    abstract::The Fragile X syndrome (FraX), which is characterized among other physical and neurologic impairments by mental retardation, is caused by the absence of the product of the FMR1 gene. The Fragile X Mental Retardation Protein (FMRP) is a member of a novel family of RNA-binding proteins. The latter includes two other pro...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.10064

    authors: Hoogeveen AT,Willemsen R,Oostra BA

    更新日期:2002-05-01 00:00:00

  • Immunolocalization of substance P and NK-1 receptor in Hofbauer cells in human normal placenta.

    abstract::Substance P (SP) after binding to the neurokinin-1 (NK-1) receptor regulates many biological functions. Both SP and the NK-1 receptor are expressed in human normal placenta cells, monocytes, and macrophages. However, to our knowledge, the presence of both SP and the NK-1 receptor in macrophages of the placenta, the Ho...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22303

    authors: Muñoz M,Carranza A,Pavón A,Anderson G,Coveñas R

    更新日期:2013-12-01 00:00:00

  • Determination of conditions for optimum resolution of a high angle thin window energy dispersive spectrometer.

    abstract::The energy resolution of an energy dispersive spectrometer (EDS) equipped with an ultrathin window (UTW) and mounted at a high take-off angle (72 degrees) on a transmission electron microscope has been studied under a variety of operating conditions. The spectrometer resolution is close to that specified by the manufa...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070220208

    authors: Blackford MG,Smith KL

    更新日期:1992-07-01 00:00:00

  • Histochemical evidence of the initial chondrogenesis and osteogenesis in the periosteum of a rib fractured model: implications of osteocyte involvement in periosteal chondrogenesis.

    abstract::We have examined cellular events at the early stages of periosteal chondrogenesis and osteogenesis induced by bone fracture, using a well-standardized rib fracture model of the mouse. The initial cellular event was recognized as considerable proliferation in the deeper layer referred to as the "cambium layer" of the p...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20088

    authors: Li M,Amizuka N,Oda K,Tokunaga K,Ito T,Takeuchi K,Takagi R,Maeda T

    更新日期:2004-07-01 00:00:00

  • Comparison of SEM processing methods for cultured human lens epithelial cells grown on flat and microcarrier bead substrates.

    abstract::The increasing importance of in vitro models has presented new challenges in SEM processing techniques. The present study has evaluated the quality of preservation of cultured human lens epithelial cells processed by critical point, Peldri II, and tert-butyl alcohol drying. Specimens processed by critical point drying...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070300508

    authors: Cantu-Crouch D,Howe WE,McCartney MD

    更新日期:1995-04-01 00:00:00

  • Nitric oxide modulates sympathetic neurotransmission at the prejunctional level.

    abstract::In spite of accumulating evidence for a modulation of sympathetic neurotransmission by endogenously produced nitric oxide (NO), it remains unclear in which parts of the vascular system and at what level this interaction takes place. The aim of the present study was to investigate the distribution of endothelial and ne...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070290214

    authors: Addicks K,Bloch W,Feelisch M

    更新日期:1994-10-01 00:00:00

  • Citron, a Rho target that affects contractility during cytokinesis.

    abstract::The small GTPase Rho, which regulates cell shape, is thought to contribute to cytokinesis. Recently, Citron was characterized as a Rho target. This large protein contains a Ser/Thr kinase domain related to that of ROCK, another Rho effector. Both endogenous Citron and recombinant Citron localize to the cleavage furrow...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(20000415)49:2<123::AID-JEM

    authors: Madaule P,Furuyashiki T,Eda M,Bito H,Ishizaki T,Narumiya S

    更新日期:2000-04-15 00:00:00

  • Biofilm formation in Haas palatal expanders with and without use of an antimicrobial agent: an in situ study.

    abstract::Orthodontic appliances causes specific alterations in oral environment, including reduction of pH, increase of dental biofilm and elevation of salivary microbial levels, causing an increased risk for dental caries. This study evaluated, using microbial culture and scanning electron microscopy (SEM), the in situ contam...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/jemt.22817

    authors: Bagatin CR,Andrucioli MCD,Ferreira JTL,Matsumoto MAN,da Silva RAB,da Silva LAB,Romano FL,Nelson-Filho P

    更新日期:2017-05-01 00:00:00

  • Assessment of mold and yeast in some bakery products of Lahore, Pakistan based on LM and SEM.

    abstract::The present investigation was designed to throw light on the microbial status of bakery products available in bakeries and supermarkets of Lahore. Different bakery samples such as biscuits, pizza, patties were collected from different localities such as Anar Kali, Chauburji, Faisal Town, Iqbal Town, Model Town, Muslim...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23103

    authors: Saeed I,Shaheen S,Hussain K,Khan MA,Jaffer M,Mahmood T,Khalid S,Sarwar S,Tahir A,Khan F

    更新日期:2019-02-01 00:00:00

  • Role of HSP70 in the regulation of the testicular apoptosis in a seasonal breeding teleost Prochilodus argenteus from the São Francisco River, Brazil.

    abstract::This study investigated the relationship among heat shock protein 70 (HSP70), proliferating cell nuclear antigen (PCNA), and testicular apoptosis during a breeding cycle of Prochilodus argenteus, a neotropical migratory characiform fish of importance in commercial fishery from the São Francisco River basin. A total of...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22173

    authors: Domingos FF,Thomé RG,Martinelli PM,Sato Y,Bazzoli N,Rizzo E

    更新日期:2013-04-01 00:00:00

  • Efficiency of spermatogenesis.

    abstract::Spermatogenesis is a process of division and differentiation by which spermatozoa are produced in seminiferous tubules. A measure of efficiency of spermatogenesis is the estimated number of spermatozoa produced per day per gram of testicular parenchyma. This measure is not influenced by species differences in testicul...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1070320504

    authors: Johnson L

    更新日期:1995-12-01 00:00:00

  • Nanoparticle embedded chitosan film for agglomeration free TEM images.

    abstract::Transmission electron microscopy (TEM) is a very useful and commonly used microscopy technique, used especially for the characterization of nanoparticles. However, the identification of the magnetic nanoparticle could be thought problematic in TEM analysis, due to the fact that the magnetic nanoparticles are usually f...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22792

    authors: Dogan Ü,Çiftçi H,Cetin D,Suludere Z,Tamer U

    更新日期:2017-02-01 00:00:00

  • Description of the immatures of workers of the ant Linepithema micans Forel (Hymenoptera: Formicidae).

    abstract::Linepithema micans Forel is a poorly studied species that is now considered a pest in vineyards in South Brazil. Larval characters have been used in a few phylogenetic studies, and their importance greatly depends in the amount of available information on different species. This study presents a complete panorama on t...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20913

    authors: Nondillo A,Solis DR,Fox EG,Rossi ML,Botton M,Bueno OC

    更新日期:2011-04-01 00:00:00

  • Histomorphological investigations on the lips of Rahmani sheep (Ovis aries): A scanning electron and light microscopic study.

    abstract::This study was conducted to provide comprehensive information on the anatomical and histological features of the upper (UL) and lower (LL) lips of Rahmani sheep by gross examination, morphometric analysis in addition to Light and scanning electron microscope (SEM). Samples from normal healthy adult male sheep heads we...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23660

    authors: Madkour FA,Mohammed ESI

    更新日期:2020-12-01 00:00:00

  • Phase contrast without phase plates and phase rings--optical solutions for improved imaging of phase structures.

    abstract::Using the optical methods described, phase specimens can be observed with a modified light microscope in enhanced clarity, purified from typical artifacts which are apparent in standard phase contrast illumination. In particular, haloing and shade-off are absent, lateral and vertical resolution are maximized and the i...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22265

    authors: Piper T,Piper J

    更新日期:2013-10-01 00:00:00

  • Microradiography as a tool to detect heavy metal uptake in plants for phytoremediation applications.

    abstract::In this paper, an application of contact microradiography with soft X-rays for detecting the uptake site of heavy metal in the whole plant leaves is investigated. The X-ray source is a laser-plasma one based on an Nd:glass laser. The soft X-ray radiation emitted from the plasma laser targets of magnesium, iron, and co...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20350

    authors: Reale L,Lai A,Bellucci I,Faenov A,Pikuz T,Flora F,Spanò L,Poma A,Limongi T,Palladino L,Ritucci A,Tomassetti G,Petrocelli G,Martellucci S

    更新日期:2006-08-01 00:00:00

  • The effect of different thermal treatment on the physical properties of PGA/PCL copolymer suture material.

    abstract::Samples of polyglycolic acid PGA/polycaprolactone PCL copolymer absorbable suture materials, which is called Poliglecaprone PGC copolymer suture, were thermally treated by two different processes. The first method is the direct heat exposure, from 20 to 38 °C, in the surrounding medium. Which is an environmental study...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22957

    authors: El-Farahaty KA,El-Bakary MA,El-Sayed NM

    更新日期:2018-01-01 00:00:00

  • Cardiac neurones of autonomic ganglia.

    abstract::The properties of the postganglionic sympathetic neurones supplying the heart and arising in the stellate and adjacent paravertebral ganglia of various species are discussed with respect to their location, morphology, synaptic input and membrane characteristics. Results from our laboratory on the morphology of rat ste...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19960901)35:1<69::AID-JEMT

    authors: Wallis D,Watson AH,Mo N

    更新日期:1996-09-01 00:00:00

  • Programmed cell death of the normal human neutrophil: an in vitro model of senescence.

    abstract::The present study provides experimental data which indicate that the neutrophil is ideal for studying programmed cell death or apoptosis in vitro. Neutrophils can be obtained from human peripheral blood in large numbers with minimal experimental manipulation and are easily separated from other leukocytes, providing ne...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070280408

    authors: Payne CM,Glasser L,Tischler ME,Wyckoff D,Cromey D,Fiederlein R,Bohnert O

    更新日期:1994-07-01 00:00:00

  • Section staining for electron microscopy using tannic acid as a mordant: a simple method for visualization of glycogen and collagen.

    abstract::The possibility that tannic acid and other classical mordants can be used in the electron microscopical section staining technique has been tested. A mordant can be defined as a chemical that combines with both a certain specific tissue component and the staining substance and thereby permits a staining reaction that ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070210110

    authors: Afzelius BA

    更新日期:1992-03-01 00:00:00

  • Application of photoconversion technique for correlated confocal and ultrastructural studies in organotypic slice cultures.

    abstract::Photoconversion of fluorescent staining into stable diaminobenzidine (DAB) precipitate is widely used for neuroanatomical and developmental studies. An important advantage of the approach is to make correlations between light and electron microscopy analyses possible, the DAB reaction product formed during photoconver...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20239

    authors: Nikonenko I,Boda B,Alberi S,Muller D

    更新日期:2005-10-01 00:00:00

  • Effect of Aloe vera application on the content and molecular arrangement of glycosaminoglycans during calcaneal tendon healing.

    abstract::Although several treatments for tendon lesions have been proposed, successful tendon repair remains a great challenge for orthopedics, especially considering the high incidence of re-rupture of injured tendons. Our aim was to evaluate the pharmacological potential of Aloe vera on the content and arrangement of glycosa...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22422

    authors: Aro AA,Esquisatto MA,Nishan U,Perez MO,Rodrigues RA,Foglio MA,Carvalho JE,Gomes L,Vidal Bde C,Pimentel ER

    更新日期:2014-12-01 00:00:00

  • Gap junctions in intestinal smooth muscle and interstitial cells of Cajal.

    abstract::This manuscript reviews gap junctions' roles in control of intestinal motility. Gap junctions (GJs) of small intestine (SmIn) are found between circular muscle (CM) cells, between interstitial cells of Cajal (ICC) of deep muscular plexus (DMP) and between them and adjacent outer circular muscle (OCM). GJs between long...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19991201)47:5<309::AID-JEM

    authors: Daniel EE,Wang YF

    更新日期:1999-12-01 00:00:00

  • Nanoscale elemental sensitivity study of Nd₂Fe₁₄B using absorption correlation tomography.

    abstract::Transmission X-ray microscopy (TXM) is a rapidly developing technique with the capability of nanoscale three dimensional (3D) real-space imaging. Combined with the wide range in energy tunability from synchrotron sources, TXM enables the retrieval of 3D microstructural information with elemental/chemical sensitivity t...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22273

    authors: Kao TL,Shi CY,Wang J,Mao WL,Liu Y,Yang W

    更新日期:2013-11-01 00:00:00

  • An optimized sample preparation approach for atomic resolution in situ studies of thin films.

    abstract::This work provides the details of a simple and reliable method with less damage to prepare electron transparent samples for in situ studies in scanning/transmission electron microscopy. In this study, we use epitaxial VO2 thin films grown on c-Al2 O3 by pulsed laser deposition, which have a monoclinic-rutile transitio...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23130

    authors: Moatti A,Sachan R,Prater J,Narayan J

    更新日期:2018-11-01 00:00:00

  • Ultrastructural study of apoptotic U937 [corrected] cells treated with different pro-apoptotic substances.

    abstract::Human monocytic leukemia U937 cells readily undergo apoptosis when exposed to various stimuli, including inhibition of protein synthesis, oxidative stress, antitumoral agents, etc. The sequential, step-by-step morphological changes in U937 cells that occur during the apoptotic program are largely determined by the act...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20058

    authors: Abbro L,Lanubile R,Dini L

    更新日期:2004-05-01 00:00:00