X-ray microanalysis of organic thin sections in TEM using an UTW Si(Li) detector: comparison of quantification methods.

Abstract:

:We compared Hall's peak to continuum ratio method with a peak ratio method in order to quantify light elements (C, N, and O) in organic specimens as a model for biological thin sections. X-ray spectra were recorded by an energy dispersive X-ray spectrometer equipped with an ultra thin window detector. Spectra were processed by means of a top-hat filter adapted to peak full-width half maximum. The peak intensities were measured by multiple least square fitting to reference spectra. For most elements of biological interest, theoretical and experimental k-factors were determined. Absorption correction was found to be important for quantitation of carbon, nitrogen, and oxygen. Boron was efficiently detected; however, quantitative analysis was not possible. We conclude from our experiments that the peak ratio method is more suitable for quantitation of elemental concentrations in biological thin sections than the peak to continuum method.

journal_name

Microsc Res Tech

authors

Laquerriere P,Banchet V,Michel J,Zierold K,Balossier G,Bonhomme P

doi

10.1002/1097-0029(20010115)52:2<231::AID-JEMT1008>

keywords:

subject

Has Abstract

pub_date

2001-01-15 00:00:00

pages

231-8

issue

2

eissn

1059-910X

issn

1097-0029

pii

10.1002/1097-0029(20010115)52:2<231::AID-JEMT1008>

journal_volume

52

pub_type

杂志文章
  • Human, rhesus macaque, and feline sequences highly similar to mouse mammary tumor virus sequences.

    abstract::Sequences highly similar (>95%) to the mouse mammary tumor virus (MMTV) env gene have been amplified from human DNA samples, including DNA samples from patients with breast cancer (BC) and persons who did not have BC. The sequences from human DNA were distinct from the MMTV sequences used as controls in these PCR reac...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20233

    authors: Szabo S,Haislip AM,Traina-Dorge V,Costin JM,Crawford BE 2nd,Wilson RB,Garry RF

    更新日期:2005-11-01 00:00:00

  • Ultrastructural characterization and immunolocalization of osteopontin in rat calvarial osteoblast primary cultures.

    abstract::As part of ongoing studies aimed at clarifying the early events of bone matrix deposition and mineralization, we have characterized primary osteoblast cultures using ultrastructural and immunocytochemical methods. Osteogenic cells were isolated by sequential enzymatic digestion of newborn rat (2-4-day-old) calvariae a...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19960201)33:2<214::AID-JEM

    authors: Nanci A,Zalzal S,Gotoh Y,McKee MD

    更新日期:1996-02-01 00:00:00

  • Assessment of mold and yeast in some bakery products of Lahore, Pakistan based on LM and SEM.

    abstract::The present investigation was designed to throw light on the microbial status of bakery products available in bakeries and supermarkets of Lahore. Different bakery samples such as biscuits, pizza, patties were collected from different localities such as Anar Kali, Chauburji, Faisal Town, Iqbal Town, Model Town, Muslim...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23103

    authors: Saeed I,Shaheen S,Hussain K,Khan MA,Jaffer M,Mahmood T,Khalid S,Sarwar S,Tahir A,Khan F

    更新日期:2019-02-01 00:00:00

  • Cytotoxic effects of Satureja montana L. essential oil on oocytes of engorged Rhipicephalus microplus female ticks (Acari: Ixodidae).

    abstract::In addition to pesticidal activity, the capacity of natural compounds to inhibit the reproduction of parasites emerge as an important alternative tick control method. In this context, Satureja spp. stand out due to their recognized pesticidal properties. Among parasites of veterinary importance, the cattle tick, Rhipi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23693

    authors: Reis AC,Konig IFM,Rezende DACS,Gonçalves RRP,Lunguinho ADS,Ribeiro JCS,Cardoso MDG,Remedio RN

    更新日期:2021-01-06 00:00:00

  • Antennal sensilla of Apanteles cypris Nixon (Hymenoptera: Braconidae), a larval endoparasitoid of Cnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae).

    abstract::Apanteles cypris is an endoparasitoid of rice pest Cnaphalocrocis medinalis. To better understand its host location mechanism, we examined the external morphology of antennal sensilla of A. cypris via scanning electron microscopy. The antennae of male and female A. cypris were filiform in shape, which consist of scape...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.21016

    authors: Zhou H,Wu WJ,Zhnag ZF,Zhnag Y

    更新日期:2011-12-01 00:00:00

  • GFAP immunoreactivity and transcription in trigeminal and dental tissues of rats and transgenic GFP/GFAP mice.

    abstract::Sensory mechanisms in teeth are not well understood and may involve pulpal-neural interactions. Tooth cells that proliferate in vitro have polyclonal immunoreactivity (IR) for glial fibrillary acidic protein (GFAP), growth-associated protein (GAP-43), and vimentin, plus glial-like ion channels. Here, we analyzed GFAP-...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20130

    authors: Byers MR,Maeda T,Brown AM,Westenbroek RE

    更新日期:2004-12-01 00:00:00

  • Cyclic fatigue and energy-dispersive X-ray spectroscopy examination of the novel ROTATE instrument.

    abstract::To investigate the cyclic fatigue resistance of novel ROTATE instruments and to compare with the same size of One Curve, TF adaptive, and Mtwo instruments using a root canal simulation model. Four-type of 25/0.06 instruments were used: ROTATE, Mtwo, One Curve, TF adaptive. Instruments were divided into two subgroups: ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23374

    authors: Ertuğrul İF,Orhan EO

    更新日期:2019-12-01 00:00:00

  • Ultrastructural and functional connectivity of intracellularly stained neurones in the vertebrate retina: correlative analyses.

    abstract::A variety of intracellular recording and staining techniques has been used to establish structure-function and, in some cases, structure-function-neurochemical correlations in fish, turtle, and cat retinae. Cone photoreceptor-horizontal cell connectivity has been studied extensively in the cyprinid fish retina by intr...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070240106

    authors: Djamgoz MB,Kolb H

    更新日期:1993-01-01 00:00:00

  • Round window membrane. Structure function and permeability: a review.

    abstract::The ultrastructure of the round window membrane of humans, monkeys, felines, and rodents discloses three basic layers: an outer epithelium, a middle core of connective tissue, and an inner epithelium. Interspecies variations are mainly in terms of thickness, being thinnest in rodents and thicker in humans. Morphologic...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19970201)36:3<201::AID-JEM

    authors: Goycoolea MV,Lundman L

    更新日期:1997-02-01 00:00:00

  • Intelligent microscopic approach for identification and recognition of citrus deformities.

    abstract::Plant diseases are accountable for economic losses in an agricultural country. The manual process of plant diseases diagnosis is a key challenge from last one decade; therefore, researchers in this area introduced automated systems. In this research work, automated system is proposed for citrus fruit diseases recognit...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23320

    authors: Safdar A,Khan MA,Shah JH,Sharif M,Saba T,Rehman A,Javed K,Khan JA

    更新日期:2019-09-01 00:00:00

  • Combination of normal light and fluorescence microscopy for authentication of five Lonicera species flower buds.

    abstract::The flower buds of five Lonicera species, Lonicera japonica Thunb., L. macranthoides Hand.-Mazz., L. hypoglauca Miq., L. confusa DC. and L. fulvotomentosa Hsu et S.C. Cheng are confusable and usually utilized under the same name "Jinyinhua" in different areas for morphological similarity. Studies found that these five...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20882

    authors: Chu C,Liu HJ,Qi LW,Liu EH,Li P

    更新日期:2011-02-01 00:00:00

  • Circadian organization and the role of the pineal in birds.

    abstract::All organisms exhibit significant daily rhythms in a myriad of functions from molecular levels to the level of the whole organism. Significantly, most of these rhythms will persist under constant conditions, showing that they are driven by an internal circadian clock. In birds the circadian system is composed of sever...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1068

    authors: Underwood H,Steele CT,Zivkovic B

    更新日期:2001-04-01 00:00:00

  • A method to isolate human limbal basal cells enriched for a subset of epithelial cells with a large nucleus/cytoplasm ratio expressing high levels of p63.

    abstract::The objectives were to develop method of isolating viable human limbal basal cells in order to enrich a subset of small cells with a large Nucleus/Cytoplasm (N/C) ratio expressing high levels of p63, nuclear protein. Limbal tissues were treated with trypsin for 50 min at 37 degrees C in an orbital shaker at 100 rpm wi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20573

    authors: Arpitha P,Prajna NV,Srinivasan M,Muthukkaruppan V

    更新日期:2008-06-01 00:00:00

  • Ultrastructural analysis of murine hippocampal neural progenitor cells in culture.

    abstract::Despite the great number of studies devoted to neural stem/progenitor cell biology, the ultrastructural characteristics of these cells in vitro have not been fully studied. To determine the fine structure of hippocampal neural progenitor cells (NPCs) in culture, mouse fetal hippocampi (E18) were extracted, dissected, ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22454

    authors: Tsupykov O

    更新日期:2015-02-01 00:00:00

  • Apoptosis of larval cells during amphibian metamorphosis.

    abstract::Programmed cell death occurs in a variety of organs during amphibian metamorphosis and is usually identified by electron microscopy as apoptosis or its modifications. Because of the massive cell death that occurs during a short period, amphibian organs serve as an ideal model system for the study of mechanisms underly...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19960615)34:3<228::AID-JEM

    authors: Ishizuya-Oka A

    更新日期:1996-06-15 00:00:00

  • Visualizing subsurface defects in graphite by acoustic atomic force microscopy.

    abstract::We describe a versatile platform, which combines atomic force acoustic microscopy, ultrasonic atomic force microscopy and heterodyne force microscopy. The AFM system can enable in-situ switching among these operation modes flexibly and thus benefit the discrimination of differences in mechanical properties and buried ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22668

    authors: Wang T,Ma C,Hu W,Chen Y,Chu J

    更新日期:2017-01-01 00:00:00

  • Role of Oct-6 in Schwann cell differentiation.

    abstract::Research into the POU transcription factor Oct-6 has been the focus of much current attention, in particular its role in Schwann cell development and differentiation. Based on published data and data presented here, we propose a model for Oct-6 function at two distinct stages of Schwann cell maturation. First, Oct-6 f...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19980601)41:5<372::AID-JEM

    authors: Jaegle M,Meijer D

    更新日期:1998-06-01 00:00:00

  • Quantitative junctional permeability measurements using the confocal microscope.

    abstract::This paper describes the use of a photobleach method and confocal microscopy to compare the cell-to-cell transfer rate of 5,6 carboxyfluorescein in dissociated embryonic chick lens cells with those in the anterior epithelium of the whole embryonic chick lens. The average cell-to-cell transfer rates obtained were 7.9 x...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070310508

    authors: Miller A

    更新日期:1995-08-01 00:00:00

  • Nanostructural analysis by atomic force microscopy followed by light microscopy on the same archival slide.

    abstract::Integrated information on ultrastructural surface texture and chemistry increasingly plays a role in the biomedical sciences. Light microscopy provides access to biochemical data by the application of dyes. Ultrastructural representation of the surface structure of tissues, cells, or macromolecules can be obtained by ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20691

    authors: Wagner M,Kaehler D,Anhenn O,Betz T,Awad S,Shamaa A,Theegarten D,Linder R

    更新日期:2009-07-01 00:00:00

  • Mechanisms of gastrulation and tail formation in ascidians.

    abstract::Ascidian embryos are useful for examining how events that occur during fertilization and cleavage affect gastrulation because they gastrulate early in development, during the seventh cleavage. In ascidians, both dorsal-ventral and anterior-posterior axes are determined before first cleavage. The dorsal-ventral axis is...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1070260403

    authors: Swalla BJ

    更新日期:1993-11-01 00:00:00

  • Macroanatomic, light and scanning electron microscopic studies of the pecten oculi in the stork (Ciconia ciconia).

    abstract::This study was undertaken to investigate the pecten oculi of stork by using macroscopic, light and electron microscopic techniques. A total of 20 eyes that were obtained from 10 storks were used. The eyes were cleaned and isolated by dissection. After various procedures, four of the pecten oculi were examined by light...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22255

    authors: Onuk B,Tutuncu S,Alan A,Kabak M,Ince NG

    更新日期:2013-09-01 00:00:00

  • Super-resolution optical microscopy based on scannable cantilever-combined microsphere.

    abstract::We report an ingenious method of super-resolution optical microscopy utilizing scannable cantilever-combined microsphere. By scanning the microsphere over the sample surface in a cantilever-combined microsphere-sample contact state, super-resolution images can be acquired at arbitrary sample regions through near-field...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22595

    authors: Wang S,Zhang D,Zhang H,Han X,Xu R

    更新日期:2015-12-01 00:00:00

  • Surface damage induced by FIB milling and imaging of biological samples is controllable.

    abstract::Focused ion beam (FIB) techniques are among the most important tools for the nanostructuring of surfaces. We used the FIB/SEM (scanning electron microscope) for milling and imaging of digestive gland cells. The aim of our study was to document the interactions of FIB with the surface of the biological sample during FI...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20494

    authors: Drobne D,Milani M,Leser V,Tatti F

    更新日期:2007-10-01 00:00:00

  • Spatial distribution of cytoskeleton intermediate filaments during fetal rat hepatocyte differentiation.

    abstract::The construction of the liver parenchyma throughout fetal development depends on the elaboration of intercellular contacts between epithelial cells and between epithelial and mesenchymal cells. During this time, the spatial distribution of cytokeratins in hepatocytes shows a striking evolution as demonstrated by confo...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19971201)39:5<436::AID-JEM

    authors: Vassy J,Irinopoulou T,Beil M,Rigaut JP

    更新日期:1997-12-01 00:00:00

  • Node distribution and packing density in the rat CNS-PNS transitional zone.

    abstract::The density of nodes of Ranvier was examined at CNS, PNS, and transitional zone (TZ) levels of rat lumbar ventral motoneurone fibres. It was found to be significantly greater in the TZ than at the other levels: The difference was sevenfold for the ventral root and at least fourfold for central fibre levels. Node distr...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19960815)34:6<507::AID-JEM

    authors: Fraher J

    更新日期:1996-08-15 00:00:00

  • Ultrastructural histopathology of human olfactory dysfunction.

    abstract::This paper presents electron-microscopic observations on biopsies of the olfactory mucosae of several classes of patients with smell disorders: 1) patients with loss of smell function following head injury (post-traumatic anosmics or hyposmics); 2) patients with loss of smell function following severe head colds and/o...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070230202

    authors: Moran DT,Jafek BW,Eller PM,Rowley JC 3rd

    更新日期:1992-10-15 00:00:00

  • The comparison between force volume and peakforce quantitative nanomechanical mode of atomic force microscope in detecting cell's mechanical properties.

    abstract::Atomic force microscope (AFM) has been widely used in the biological field owing to its high sensitivity (subnanonewton), high spatial resolution (nanometer), and adaptability to physiological environments. Nowadays, force volume (FV) and peakforce quantitative nanomechanical (QNM) are two distinct modes of AFM used i...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23351

    authors: Yang Y,Xiao X,Peng Y,Yang C,Wu S,Liu Y,Yue T,Pu H,Liu N,Jiang H

    更新日期:2019-11-01 00:00:00

  • Investigation of mineral content of root canal dentin after the application of various antibiotic paste using energy-dispersive X-ray detector.

    abstract::The purpose of this study was to evaluate mineral content of root canal dentin after treatment with different antibiotic pastes including the mixture of metronidazole, ciprofloxacin, doxycycline, cefaclor, amoxicillin, or minocycline. Fifty extracted maxillary canine teeth were randomly divided into five groups (n = 1...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23155

    authors: Simsek H,Coruh M,Cakici F,Fundaoglu Kucukekenci F,Gurbuz T,Cakici EB

    更新日期:2019-02-01 00:00:00

  • 3D reconstruction of high-resolution STED microscope images.

    abstract::Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100 nm with focu...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20602

    authors: Punge A,Rizzoli SO,Jahn R,Wildanger JD,Meyer L,Schönle A,Kastrup L,Hell SW

    更新日期:2008-09-01 00:00:00

  • Animal models are reliably mimicking human diseases? A morphological study that compares animal with human NAFLD.

    abstract::Non-alcoholic fatty liver disease (NAFLD) is a clinical-pathological syndrome that includes a wide spectrum of morphological alterations. In research, animal models are crucial in evaluating not only the pathogenesis of NAFLD and its progression, but also the therapeutic effects of various agents. Investigations on th...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22401

    authors: Solinas P,Isola M,Lilliu MA,Conti G,Civolani A,Demelia L,Loy F,Isola R

    更新日期:2014-10-01 00:00:00