Abstract:
:We compared Hall's peak to continuum ratio method with a peak ratio method in order to quantify light elements (C, N, and O) in organic specimens as a model for biological thin sections. X-ray spectra were recorded by an energy dispersive X-ray spectrometer equipped with an ultra thin window detector. Spectra were processed by means of a top-hat filter adapted to peak full-width half maximum. The peak intensities were measured by multiple least square fitting to reference spectra. For most elements of biological interest, theoretical and experimental k-factors were determined. Absorption correction was found to be important for quantitation of carbon, nitrogen, and oxygen. Boron was efficiently detected; however, quantitative analysis was not possible. We conclude from our experiments that the peak ratio method is more suitable for quantitation of elemental concentrations in biological thin sections than the peak to continuum method.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Laquerriere P,Banchet V,Michel J,Zierold K,Balossier G,Bonhomme Pdoi
10.1002/1097-0029(20010115)52:2<231::AID-JEMT1008>keywords:
subject
Has Abstractpub_date
2001-01-15 00:00:00pages
231-8issue
2eissn
1059-910Xissn
1097-0029pii
10.1002/1097-0029(20010115)52:2<231::AID-JEMT1008>journal_volume
52pub_type
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