Abstract:
:Focused ion beam (FIB) techniques are among the most important tools for the nanostructuring of surfaces. We used the FIB/SEM (scanning electron microscope) for milling and imaging of digestive gland cells. The aim of our study was to document the interactions of FIB with the surface of the biological sample during FIB investigation, to identify the classes of artifacts, and to test procedures that could induce the quality of FIB milled sections by reducing the artifacts. The digestive gland cells were prepared for conventional SEM. During FIB/SEM operation we induced and enhanced artifacts. The results show that FIB operation on biological tissue affected the area of the sample where ion beam was rastering. We describe the FIB-induced surface major artifacts as a melting-like effect, sweating-like effect, morphological deformations, and gallium (Ga(+)) implantation. The FIB induced surface artifacts caused by incident Ga(+) ions were reduced by the application of a protective platinum strip on the surface exposed to the beam and by a suitable selection of operation protocol. We recommend the same sample preparation methods, FIB protocol for milling and imaging to be used also for other biological samples.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Drobne D,Milani M,Leser V,Tatti Fdoi
10.1002/jemt.20494subject
Has Abstractpub_date
2007-10-01 00:00:00pages
895-903issue
10eissn
1059-910Xissn
1097-0029journal_volume
70pub_type
杂志文章abstract::Transmission X-ray microscopy (TXM) is a rapidly developing technique with the capability of nanoscale three dimensional (3D) real-space imaging. Combined with the wide range in energy tunability from synchrotron sources, TXM enables the retrieval of 3D microstructural information with elemental/chemical sensitivity t...
journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
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journal_title:Microscopy research and technique
pub_type: 杂志文章
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journal_title:Microscopy research and technique
pub_type: 杂志文章
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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更新日期:2005-07-01 00:00:00
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
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journal_title:Microscopy research and technique
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更新日期:2019-12-01 00:00:00
abstract::The glycoconjugate pattern of developing ovarian follicles in wild and cultured Senegalese sole Solea senegalensis was investigated by means of lectin histochemistry. Ovaries from cultured fish contained oocytes up to the late vitellogenic stage, whereas they reached the hydration stage in wild specimens. The follicul...
journal_title:Microscopy research and technique
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