Abstract:
:Fatty acid and alkyl compositions of 1-alkyl-2,3-diacylglycerols (ADG) in the Harderian gland of the golden hamster were determined. Fatty acids of male ADG consisted of straight chain saturated acids ranging from C12 to C22. Both even- and odd-numbered acids were observed, indicating that acetyl- and propionyl-CoAs were equally used as primers in the fatty acid synthesis. In female ADG a large amount of iso- and anteiso-branched fatty acids were detected. Odd-numbered acids (C15, 17, 19, and 21) contained iso- together with anteiso-branchings, and even-numbered acids (C16 and 18) contained iso-branchings. These findings suggested that isobutyryl-, isovaleryl-, and 2-methylbutyryl-CoAs were used as primers in addition to acetyl- and propionyl-CoAs in fatty acid synthesis in the female gland. Such unusual primers are catabolic intermediates of valine, leucine, and isoleucine, respectively. Male ADG contained straight chain saturated alkyl residues ranging from C14 to 21. In the case of female ADG, though the main component was C18:O, most alkyl components had branchings at the iso- and anteiso-positions. NMR data suggested the presence of propionic acid at the C3 position of glycerol in the male ADG and of isovaleric and 2-methylbutyric acids at the same position in the female ADG. These results suggest that the sexual differences of the lipids in the Harderian gland of the golden hamster are determined at the step of fatty acid synthesis depending on the available precursors in the male and female glands. We speculate that sex hormones affect the metabolism of branched chain amino acids and bring about the dramatic diversities of the lipid compositions in the male and female glands.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Seyama Y,Otsuka H,Ohashi K,Vivien-Roels B,Pevet Pdoi
10.1002/(SICI)1097-0029(19960501)34:1<71::AID-JEMTsubject
Has Abstractpub_date
1996-05-01 00:00:00pages
71-6issue
1eissn
1059-910Xissn
1097-0029pii
10.1002/(SICI)1097-0029(19960501)34:1<71::AID-JEMTjournal_volume
34pub_type
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