Abstract:
:Detailed maps of the A-T distribution within human mitotic chromosomes labeled with BrdU are obtained with a high resolution scanning ion microprobe through the detection of bromine by imaging secondary ion mass spectrometry (SIMS). Corresponding maps of the emission loci of the molecular ion CN- describe the overall DNA, RNA and protein distribution in the chromosomes. Several chromosome preparations exhibit base-specific banding patterns (SIMS-bands) which mimic the well known G- or Q-bands resulting from conventional staining methods for optical microscopy. SIMS-bands are more noticeable in mitotic cells at the first cell cycle and after in situ denaturation or Giemsa staining. Sister chromatid exchanges (SCE) at the second cell cycle and beyond, occurring both spontaneously and promoted following cell culture exposure to the chemical aphidicolin (an inhibitor of DNA replication), can be visualized readily from the relative label signal intensities between sister chromatids. The comparison of base-specific label maps with CN- maps, in conjunction with the appearance of base-specific banding patterns, is informative about protein survival and/or removal following different chromosome preparation protocols. In addition, the resulting condensation state of the chromosomes can be appraised during SIMS analysis from the sample topography (imaged via the collection of mass-unresolved secondary ions). We demonstrate that imaging SIMS is a powerful complement to existing methods for the study of banding mechanisms and for the elucidation of chromosome structure. The advantages of this novel approach to the systematic and quantitative study of cytogenetic phenomena and methodologies are still largely untapped.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Levi-Setti R,Chabala JM,Gavrilov K,Espinosa R 3rd,Le Beau MMdoi
10.1002/(SICI)1097-0029(19970215)36:4<301::AID-JEMsubject
Has Abstractpub_date
1997-02-15 00:00:00pages
301-12issue
4eissn
1059-910Xissn
1097-0029pii
10.1002/(SICI)1097-0029(19970215)36:4<301::AID-JEMjournal_volume
36pub_type
杂志文章abstract::Growth rings were used to determine the root age of medicinal Paeonia lactiflora from four producing areas, and their corresponding paeoniflorin content were measured based on the identification of ages. Different P. lactiflora root samples of different ages were collected from the four major growing areas in China: B...
journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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pub_type: 杂志文章
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更新日期:2008-09-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章,多中心研究
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更新日期:1995-10-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
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journal_title:Microscopy research and technique
pub_type: 杂志文章
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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