Abstract:
:The preparation of optically clear, thick sections of fragile embryonic tissues greatly aids the power of confocal scanning laser microscopy in imaging three-dimensional structures. We report here conditions for embedding, sectioning, and staining embryos in polyacrylamide gels for a variety of confocal imaging techniques. Infiltration of tissues in standard mixtures of 10-15% acrylamide monomer yields, upon polymerization, blocks that cut easily by vibratome between 50 and 1,000 microns. These conditions worked well for tissues previously stained or for staining gel sections with low molecular weight water-soluble fluorochromes (MW < 5 kD [e.g., propidium iodide, phalloidin]). For immunostaining of tissue after embedding and sectioning, the acrylamide concentration was reduced to 2-3% acrylamide to allow access of immunoglobulins to antigenic sites; such gels were supplemented with 1% agarose to facilitate sectioning and handling. Either method yielded abundant, optically clear, and easily handled sections for mounting and examination in water-miscible media.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Germroth PG,Gourdie RG,Thompson RPdoi
10.1002/jemt.1070300608subject
Has Abstractpub_date
1995-04-15 00:00:00pages
513-20issue
6eissn
1059-910Xissn
1097-0029journal_volume
30pub_type
杂志文章abstract::Mutations in genes related to long QT syndrome (LQTS) is recognized as an independent risk of drug-induced LQTS. We previously screened a mutation F463L in a Chinese patient with LQT2, syncope, and epilepsy. Here, we planned to illustrate how F463L influences the action of dofetilide on hERG channels. F463L-hERG plasm...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23021
更新日期:2018-06-01 00:00:00
abstract::The neurotrophins are a diverse family of peptides which activate specific tyrosine kinase-linked receptors. Over the past five decades, since the pioneering work of Levi-Montalcini and colleagues, the critical role that neurotrophins play in shaping the developing nervous system has become increasingly established. T...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19990515/01)45:4/5<276::AI
更新日期:1999-05-15 00:00:00
abstract::Microscopic imaging of uneven surfaces is difficult because of the limited depth of field. In this study, we developed a rapid auto-focus method for uneven surfaces based on image fusion. The Prewitt operator was used to detect the vertical edges of the images. Then, the focus position was theoretically calculated usi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23328
更新日期:2019-09-01 00:00:00
abstract::Studies on embryonic development in vitro as well as observations in vivo, suggested that two aspects of oviduct physiology are important for early development. On one side has to be considered the oviduct "environment": temperature, pH, osmotic pressure, nutrients, oxygen tension, free radical scavengers, etc. On the...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070320102
更新日期:1995-09-01 00:00:00
abstract::Based on direct reducing of copper ions by commercially available zero valent iron (ZVI), ZVI/Cu polyscale particles with high activation are synthesized and exhibit significant removal efficiency for reactive black (RB) dye. The ZVI/Cu polyscale particles could maintain a good performance for RB removal at a wide pH ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23152
更新日期:2019-02-01 00:00:00
abstract::This article focusses on the structural development of human esophageal ciliated epithelium. A combination of transmission electron microscopic (TEM), scanning electron microscopic (SEM), radioautographic, and light microscopic (LM) analyses were carried out using intact fetal tissues between 8 and 20 weeks of gestati...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070310305
更新日期:1995-06-15 00:00:00
abstract::A synchrotron radiation fiber diffraction structural study of the axial filament of siliceous spicules from two species of marine sponges (the Demosponge Geodia cydonium and the Hexactinellid Scolymastra joubini) was carried out. The sharpness of the spots in the diffraction patterns indicated that the protein units i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10403
更新日期:2003-11-01 00:00:00
abstract::Immunohistochemistry as well as immunohistofluorescence were used to investigate the distribution of the neurotrophin-3 (NT3) in the adult rat cochlear nucleus. We found a widespread distribution of NT3 immunolabeled neurons throughout the three divisions of this nucleus. NT3-like immunoreactivity was clearly populati...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980501)41:3<224::AID-JEM
更新日期:1998-05-01 00:00:00
abstract::The anatomical substrate for coordinated release from the dispersed gonadotropin-releasing hormone (GnRH) neuronal population remains obscure. There is physiological evidence that the GnRH hormone itself has a role in tonic inhibition or modulation of GnRH function. This has led to the hypothesis that there is an ultr...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19990101)44:1<11::AID-JEMT
更新日期:1999-01-01 00:00:00
abstract::The autonomic neuromuscular junction at a varicosity in the vas deferens is defined by the localization of the vesicle-associated protein syntaxin in high concentrations in the axolemma and a high density of P2x1 receptors in a cluster beneath the varicosity. Calcium fluxes have been observed in all individual varicos...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19980915)42:6<433::AID-JEM
更新日期:1998-09-15 00:00:00
abstract::The properties of the postganglionic sympathetic neurones supplying the heart and arising in the stellate and adjacent paravertebral ganglia of various species are discussed with respect to their location, morphology, synaptic input and membrane characteristics. Results from our laboratory on the morphology of rat ste...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19960901)35:1<69::AID-JEMT
更新日期:1996-09-01 00:00:00
abstract::Superior cervical ganglion neurons (SCGNs) were isolated from 7-day-old rat SCG and cultured in MEM containing horse serum, fetal calf serum, and nerve growth factor. In this culture condition, it is well known that the SCGNs form cholinergic synapse. In 3-4 weeks cultured neurons, immunofluorescent staining for synap...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070290206
更新日期:1994-10-01 00:00:00
abstract::The synaptic organization of the amphibian sympathetic ganglia was studied, especially in the last two abdominal paravertebral ganglia of the frog. These ganglia appear to form a monosynaptic relay, not containing interneurons. They consist of two systems working in parallel: the principal neurons, by far the most num...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19961001)35:2<157::AID-JEM
更新日期:1996-10-01 00:00:00
abstract::The morphology of pancreatic excretory duct segments was reviewed in mammals. The fine structure of the epithelial lining was described in intercalated ducts, intra- and extralobular ducts, and in major pancreatic ducts. Morphological characteristics of the various cell types comprising to the duct epithelium were det...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970601)37:5/6<407::AID-J
更新日期:1997-06-01 00:00:00
abstract::Diestian ferruginous sandstone has been used as the dominant building stone for monuments in the Hageland, a natural landscape in east-central Belgium. Like all rocks, this stone type is sensitive to weathering. Case hardening was observed in combination with blackening of the exterior parts of the dressed stones. To ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20987
更新日期:2011-11-01 00:00:00
abstract::Fatty acid and alkyl compositions of 1-alkyl-2,3-diacylglycerols (ADG) in the Harderian gland of the golden hamster were determined. Fatty acids of male ADG consisted of straight chain saturated acids ranging from C12 to C22. Both even- and odd-numbered acids were observed, indicating that acetyl- and propionyl-CoAs w...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19960501)34:1<71::AID-JEMT
更新日期:1996-05-01 00:00:00
abstract::The dermal denticles are among the unique morphological adaptations of sharks, which have been acquired throughout their long evolutionary process of more than 400 million years. Species-specific morphological characteristics of these structures has been applied specially as tools for functional and taxonomic (family-...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23273
更新日期:2019-08-01 00:00:00
abstract::A new and improved technique for microvascular corrosion casting was developed and verified by examination of corrosion casts of 90 bovine limbs. The described technique renders a complete filling of the vasculature of the claw even in regions that hitherto proved to be difficult regarding completeness of filling such...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990501)45:3<184::AID-JEM
更新日期:1999-05-01 00:00:00
abstract::Sequences highly similar (>95%) to the mouse mammary tumor virus (MMTV) env gene have been amplified from human DNA samples, including DNA samples from patients with breast cancer (BC) and persons who did not have BC. The sequences from human DNA were distinct from the MMTV sequences used as controls in these PCR reac...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20233
更新日期:2005-11-01 00:00:00
abstract::Malaria is a serious worldwide disease, caused by a bite of a female Anopheles mosquito. The parasite transferred into complex life round in which it is grown and reproduces into the human body. The detection and recognition of Plasmodium species are possible and efficient through a process called staining (Giemsa). T...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23269
更新日期:2019-07-01 00:00:00
abstract::Using high resolution transmission electron microscopy with image simulation and Fourier analysis, the Li1- x FePO4 (x < 0.01), Li1- x FePO4 (x ∼ 0.5), and FePO4 particles, prepared by charging or discharging the 053048 electrochemical cells (thickness: 5 mm, width: 30 mm, height: 48 mm) and dismantled inside an Ar-fi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22495
更新日期:2015-06-01 00:00:00
abstract::In a previous report we described the survival and contractile function of mouse embryonic stem cell-derived cardiomyocytes in the host retroperitoneum. To further understand the nature of embryonic stem cell-derived cardiomyocytes, the study assessed the synthesis of natriuretic peptides in ectopic myocardial tissues...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20152
更新日期:2005-03-01 00:00:00
abstract::Transgenic mice are valuable experimental models of human endocrine diseases. Targeted ablation of specific cell lineages or insertion of genes coding for releasing factors, hormones, growth factors, and oncogenes fused with appropriate promoters, or mutated genes, can induce several pituitary disorders. Various hypos...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19971015)39:2<194::AID-JEM
更新日期:1997-10-15 00:00:00
abstract::Chronic kidney disease (CKD) is a relevant disease in feline clinic. The tubulointerstitial damage, with collagen deposition and fibrosis, is an important result of this process. The aim of this study was to quantify and correlate the deposition of collagen and severity of interstitial fibrosis (IF) in the kidney from...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22830
更新日期:2017-05-01 00:00:00
abstract::Bats are the only mammals that can fly in the dark without eye usage. This study was conducted to describe the structural and functional adaptations of the retina of two bats very common in the Egyptian fauna having a different lifestyle: the Egyptian fruit bat (Rousettus aegyptiacus) and insectivorous bat (Pipistrell...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23530
更新日期:2020-11-01 00:00:00
abstract::As part of ongoing studies aimed at clarifying the early events of bone matrix deposition and mineralization, we have characterized primary osteoblast cultures using ultrastructural and immunocytochemical methods. Osteogenic cells were isolated by sequential enzymatic digestion of newborn rat (2-4-day-old) calvariae a...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19960201)33:2<214::AID-JEM
更新日期:1996-02-01 00:00:00
abstract::STM tips made from antiferromagnetic MnNi have been used to investigate the atomic structure of the (001) and (111) surfaces of Fe3O4. The clean (001) surface displays a ( radical2 x radical2)R45 degrees superlattice, which is attributed to charge-ordering on the surface, where Fe(2+)-Fe2+ and Fe(3+)-Fe3+ dimers can b...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20148
更新日期:2005-02-01 00:00:00
abstract::The subcommissural organ (SCO) is a brain circumventricular organ formed by ependymal and hypendymal secretory cells. It secretes glycoproteins into the cerebrospinal fluid of the third ventricle where they condense into a thread-like structure known as Reissner's fiber (RF). The present study was designed to investig...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/1097-0029(20010301)52:5<496::AID-JEMT1035>
更新日期:2001-03-01 00:00:00
abstract::Brain tumor identification using magnetic resonance images (MRI) is an important research domain in the field of medical imaging. Use of computerized techniques helps the doctors for the diagnosis and treatment against brain cancer. In this article, an automated system is developed for tumor extraction and classificat...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23238
更新日期:2019-06-01 00:00:00
abstract::High-angle annular dark-field or Z-contrast microscopy was used to demonstrate that well dispersed metal supported catalysts consist of nanometer sized clusters. Depending upon the impregnated metal, different cluster sizes were observed. Grouping of Pd clusters could also be confirmed by analytical electron microscop...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070280509
更新日期:1994-08-01 00:00:00