Confocal microscopy of thick sections from acrylamide gel embedded embryos.


:The preparation of optically clear, thick sections of fragile embryonic tissues greatly aids the power of confocal scanning laser microscopy in imaging three-dimensional structures. We report here conditions for embedding, sectioning, and staining embryos in polyacrylamide gels for a variety of confocal imaging techniques. Infiltration of tissues in standard mixtures of 10-15% acrylamide monomer yields, upon polymerization, blocks that cut easily by vibratome between 50 and 1,000 microns. These conditions worked well for tissues previously stained or for staining gel sections with low molecular weight water-soluble fluorochromes (MW < 5 kD [e.g., propidium iodide, phalloidin]). For immunostaining of tissue after embedding and sectioning, the acrylamide concentration was reduced to 2-3% acrylamide to allow access of immunoglobulins to antigenic sites; such gels were supplemented with 1% agarose to facilitate sectioning and handling. Either method yielded abundant, optically clear, and easily handled sections for mounting and examination in water-miscible media.


Microsc Res Tech


Germroth PG,Gourdie RG,Thompson RP




Has Abstract


1995-04-15 00:00:00












  • Role of parietal and principal gastric mucosa cells in the phenomenon of concentration of aluminum and indium.

    abstract::The subcellular behavior of aluminum and indium, used in medical and industrial fields, was studied in the gastric mucosa and the liver after their intragastric administration to rats, using, two of the most sensitive methods of observation and microanalysis, the transmission electron microscopy, and the secondary ion...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Maghraoui S,Ayadi A,Audinot JN,Ben Ammar A,Jaafoura MH,El Hili A,Migeon HN,Tekaya L

    更新日期:2012-02-01 00:00:00

  • Effect of ultrasonic excitation on the porosity of glass ionomer cement: a scanning electron microscope evaluation.

    abstract:BACKGROUND:Ultrasonic excitation (US) was applied to glass ionomer cement (GIC) during early set time to increase the advantageous properties of this material. PURPOSE:The aim of this in vitro study was to assess the inner porosity of GIC after US. STUDY DESIGN:A total of 16 specimens, for each material, were prepare...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Coldebella CR,Santos-Pinto L,Zuanon AC

    更新日期:2011-01-01 00:00:00

  • Ultrastructure and three-dimensional study of the lymphatic stomata in the costal pleura of the rabbit.

    abstract::The aim of this report was to investigate the ultrastructure and three-dimensional organization of the pleural lymphatic stomata in the adult rabbit costal pleura by electron microscopy. A computer image processing system attached to the scanning electron microscopy was used to get statistical evaluation of the dimens...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Li YY,Li JC

    更新日期:2003-10-15 00:00:00

  • Histological description of immature Chironomus columbiensis (Diptera: Chironomidae): A potential contribution to environmental monitoring.

    abstract::Chironomids have been widely used in environmental monitoring and toxicity assays. Assessment criteria for chironomids range from the molecular-biochemical level to the population level. However, the use of markers to evaluate cellular and anatomical changes in organs and systems of individuals under contaminant expos...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Ospina-Pérez EM,Campeón-Morales OI,Richardi VS,Rivera-Páez FA

    更新日期:2019-08-01 00:00:00

  • Diabetic retinopathy detection and classification using hybrid feature set.

    abstract::Complicated stages of diabetes are the major cause of Diabetic Retinopathy (DR) and no symptoms appear at the initial stage of DR. At the early stage diagnosis of DR, screening and treatment may reduce vision harm. In this work, an automated technique is applied for detection and classification of DR. A local contrast...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Amin J,Sharif M,Rehman A,Raza M,Mufti MR

    更新日期:2018-09-01 00:00:00

  • Laser-based in situ techniques: novel methods for generating extreme conditions in TEM samples.

    abstract::The dynamic transmission electron microscope (DTEM) is introduced as a novel tool for in situ processing of materials. Examples of various types of dynamic studies outline the advantages and differences of laser-based heating in the DTEM in comparison to conventional (resistive) heating in situ TEM methods. We demonst...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Taheri ML,Lagrange T,Reed BW,Armstrong MR,Campbell GH,DeHope WJ,Kim JS,King WE,Masiel DJ,Browning ND

    更新日期:2009-03-01 00:00:00

  • Telocytes in the hearts of Saanen goats.

    abstract::Telocytes, new interstitial cells that have received significant attention in recent years, have been detected in many organs, including the heart. The distinction between telocytes and other interstitial cells can only be made based on their ultrastructural characterization and immunophenotypic features. In this stud...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Selviler-Sizer S,Kabak YB,Kabak M

    更新日期:2020-10-05 00:00:00

  • Quantitative junctional permeability measurements using the confocal microscope.

    abstract::This paper describes the use of a photobleach method and confocal microscopy to compare the cell-to-cell transfer rate of 5,6 carboxyfluorescein in dissociated embryonic chick lens cells with those in the anterior epithelium of the whole embryonic chick lens. The average cell-to-cell transfer rates obtained were 7.9 x...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Miller A

    更新日期:1995-08-01 00:00:00

  • Fluorescent human lung macrophages analyzed by spectral confocal laser scanning microscopy and multispectral cytometry.

    abstract::Numerous highly fluorescent macrophages (MPhi), designated "smoker cells," exist in the lungs of smokers and subjects who have quit smoking within 5 years. The brightly fluorescent MPhi, however, are not present in the lungs of never smokers. Some investigators have speculated that the intense fluorescence of the MPhi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Pauly JL,Allison EM,Hurley EL,Nwogu CE,Wallace PK,Paszkiewicz GM

    更新日期:2005-06-01 00:00:00

  • Action of andiroba oil (Carapa guianensis) on Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) semi-engorged females: morphophysiological evaluation of reproductive system.

    abstract::Because of the increasing medical-veterinary importance of ticks, the development of alternative control methods, less aggressive to the host and the environment has become the target of several researches. In this sense, the present study analyzed the action of different concentrations (5, 10, and 20%) of andiroba se...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Vendramini MC,Mathias MI,De Faria AU,Furquim KC,De Souza LP,Bechara GH,Roma GC

    更新日期:2012-12-01 00:00:00

  • Dynamic structural changes of synaptic contacts in the visual system of insects.

    abstract::The visual system of insects provides an excellent model to study processes of transduction and transmission of photic information, synaptogenesis, synaptic plasticity, and wiring between photoreceptors and their visual interneurons in the optic lobe. This review describes synaptic contacts between photoreceptors and ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审


    authors: Pyza E

    更新日期:2002-08-15 00:00:00

  • Super-resolution optical microscopy based on scannable cantilever-combined microsphere.

    abstract::We report an ingenious method of super-resolution optical microscopy utilizing scannable cantilever-combined microsphere. By scanning the microsphere over the sample surface in a cantilever-combined microsphere-sample contact state, super-resolution images can be acquired at arbitrary sample regions through near-field...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Wang S,Zhang D,Zhang H,Han X,Xu R

    更新日期:2015-12-01 00:00:00

  • Histochemical evidence of the initial chondrogenesis and osteogenesis in the periosteum of a rib fractured model: implications of osteocyte involvement in periosteal chondrogenesis.

    abstract::We have examined cellular events at the early stages of periosteal chondrogenesis and osteogenesis induced by bone fracture, using a well-standardized rib fracture model of the mouse. The initial cellular event was recognized as considerable proliferation in the deeper layer referred to as the "cambium layer" of the p...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Li M,Amizuka N,Oda K,Tokunaga K,Ito T,Takeuchi K,Takagi R,Maeda T

    更新日期:2004-07-01 00:00:00

  • Granulated metrial gland cells: a natural killer cell subset of the pregnant murine uterus.

    abstract::The metrial gland develops in the uterus of many rodent species during normal pregnancy. It is a maternally-derived tissue that contains stromal and vascular elements plus a population of large cells, striking in their light microscopic appearance due to the presence of numerous cytoplasmic granules. These cells, whic...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审


    authors: Croy BA,Kiso Y

    更新日期:1993-06-15 00:00:00

  • Anatomy of the frontal gland and ultramorphology of the frontal tube in the soldier caste of species of Nasutitermitinae (Isoptera, Termitidae).

    abstract::All termite species (Isoptera) are eusocial. These insects live in societies where the division of labor is a predominant characteristic, conditioned by the presence of castes with different morphology, ontogeny, and development. The soldier caste is unique among social insects and it is responsible for colony defense...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Santos CA,Costa-Leonardo AM

    更新日期:2006-11-01 00:00:00

  • Anatomical and molecular design of the Drosophila antenna as a flagellar auditory organ.

    abstract::The molecular basis of hearing is less well understood than many other senses. However, recent studies in Drosophila have provided some important steps towards a molecular understanding of hearing. In this report, we summarize these findings and their implications on the relationship between hearing and touch. In Dros...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审


    authors: Todi SV,Sharma Y,Eberl DF

    更新日期:2004-04-15 00:00:00

  • Analysis of dynamic cantilever behavior in tapping mode atomic force microscopy.

    abstract::Tapping mode atomic force microscopy (AFM) provides phase images in addition to height and amplitude images. Although the behavior of tapping mode AFM has been investigated using mathematical modeling, comprehensive understanding of the behavior of tapping mode AFM still poses a significant challenge to the AFM commun...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Deng W,Zhang GM,Murphy MF,Lilley F,Harvey DM,Burton DR

    更新日期:2015-10-01 00:00:00

  • Development of pigment cells in the zebrafish embryo.

    abstract::In recent years, the zebrafish Danio rerio has emerged as a powerful model organism for the study of vertebrate embryogenesis. Zebrafish, like other vertebrates, possess pigment cells that arise from two distinct embryonic sources: those of the dermis and epidermis originate from the neural crest, while those that com...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审


    authors: Lister JA

    更新日期:2002-09-15 00:00:00

  • Detection of membrane-bound tumor necrosis factor (TNF): an analysis of TNF-specific reagents.

    abstract::Tumor necrosis factor (TNF) exists in two bioactive forms, the membrane integrated form and the proteolytically derived soluble cytokine. Both forms of TNF are involved in a variety of different physiological and pathophysiological situations. Here we analyzed different human and mouse TNF-specific reagents for their ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Gerspach J,Götz A,Zimmermann G,Kolle C,Böttinger H,Grell M

    更新日期:2000-08-01 00:00:00

  • The transformation of phytolith morphology as the result of their exposure to high temperature.

    abstract::Phytoliths are an important component for interpreting the ancient botanical record. However, phytoliths can be altered through heating, either as the result of such activities as firing ceramics, clay molds use for casting metal or in hearths. Phytoliths can also be altered through heating as the result of creating c...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Wu Y,Wang C,Hill DV

    更新日期:2012-07-01 00:00:00

  • Phase contrast without phase plates and phase rings--optical solutions for improved imaging of phase structures.

    abstract::Using the optical methods described, phase specimens can be observed with a modified light microscope in enhanced clarity, purified from typical artifacts which are apparent in standard phase contrast illumination. In particular, haloing and shade-off are absent, lateral and vertical resolution are maximized and the i...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Piper T,Piper J

    更新日期:2013-10-01 00:00:00

  • Effect of indirubin-3-monoxime against lung cancer as evaluated by histological and transmission electron microscopic studies.

    abstract::The aim of this study is to evaluate the antitumor effect of indirubin-3-monoxime and its mode of action in benzo(α)pyrene [B(α)P] induced lung cancer in A/J mice. Light microscopic examination of lung sections of [B(α)P] induced lung cancer mice revealed the presence of adenocarcinoma characterized by extensive proli...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Ravichandran K,Pal A,Ravichandran R

    更新日期:2010-10-01 00:00:00

  • Sexual diversity of the lipid metabolism in the Harderian gland of the golden hamster.

    abstract::Fatty acid and alkyl compositions of 1-alkyl-2,3-diacylglycerols (ADG) in the Harderian gland of the golden hamster were determined. Fatty acids of male ADG consisted of straight chain saturated acids ranging from C12 to C22. Both even- and odd-numbered acids were observed, indicating that acetyl- and propionyl-CoAs w...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Seyama Y,Otsuka H,Ohashi K,Vivien-Roels B,Pevet P

    更新日期:1996-05-01 00:00:00

  • Investigation of mineral content of root canal dentin after the application of various antibiotic paste using energy-dispersive X-ray detector.

    abstract::The purpose of this study was to evaluate mineral content of root canal dentin after treatment with different antibiotic pastes including the mixture of metronidazole, ciprofloxacin, doxycycline, cefaclor, amoxicillin, or minocycline. Fifty extracted maxillary canine teeth were randomly divided into five groups (n = 1...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Simsek H,Coruh M,Cakici F,Fundaoglu Kucukekenci F,Gurbuz T,Cakici EB

    更新日期:2019-02-01 00:00:00

  • Structure, assembly, and dynamics of actin filaments in situ and in vitro.

    abstract::Actin, though highly conserved, exhibits a myriad of diverse functions, most of which ultimately depend on its intrinsic ability to rapidly assemble and disassemble filamentous structures. Many organisms synthesize multiple actin isoforms even within the same cell. Tissue-specific expression patterns and tight develop...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审


    authors: Schoenenberger CA,Steinmetz MO,Stoffler D,Mandinova A,Aebi U

    更新日期:1999-10-01 00:00:00

  • Time-series investigation of fused vesicles in microvessel endothelial cells with atomic force microscopy.

    abstract::Vesicles or caveolae within endothelial cells, fusing together to form vacuolar organelles, are implicated in macromolecular transport and cellular element transmigration across the blood-brain barrier (BBB) during inflammation and ischemia. Vacuolar organelles have been described by transmission electron microscopy a...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Chen L,Chu W,Xu Y,Chen P,Lao F,Sun Q,Feng X,Han D

    更新日期:2010-02-01 00:00:00

  • Sugarcane cell wall structure and lignin distribution investigated by confocal and electron microscopy.

    abstract::Lignocellulosic plant cell wall is considered a potential source for second generation biofuels. The plant cell wall is a highly complex structure mainly composed of cellulose, hemicelluloses, and lignin that form a network of crosslinked fibers. The structural organization of the sugarcane cell wall has not been prev...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Sant'Anna C,Costa LT,Abud Y,Biancatto L,Miguens FC,de Souza W

    更新日期:2013-08-01 00:00:00

  • Image processing techniques in a preliminary morphometric characterization of corneal epithelium by scanning electron microscopy.

    abstract::Several processing techniques of digital images allowed us to quantify the percentage of cell surface covered by microprojections (microvilli or microplicae) (SCM), the adhesion between epithelial cells by the parameter intercellular junctions (IJ), the size (cell area), shape (cell shape) and shade (cell shade) of ce...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Julio G,Merindano D,Canals M,Ralló M

    更新日期:2010-10-01 00:00:00

  • Synchronized neuronal networks: the GnRH system.

    abstract::The anatomical substrate for coordinated release from the dispersed gonadotropin-releasing hormone (GnRH) neuronal population remains obscure. There is physiological evidence that the GnRH hormone itself has a role in tonic inhibition or modulation of GnRH function. This has led to the hypothesis that there is an ultr...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审


    authors: Witkin JW

    更新日期:1999-01-01 00:00:00

  • Acute effects of splint immobilization of the forearm on in vivo microcirculation and histomorphology of the human skin.

    abstract:BACKGROUND:Splint immobilization of the forearm is often performed in clinical practice. Previous studies investigated the effect of immobilization on bone, cartilage, muscle, and tendon, however, the acute effects on human skin microcirculation and histomorphology remains elusive. METHODS:In 12 healthy, nonsmoking in...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章


    authors: Altintas AA,Vogt PM,Altintas MA

    更新日期:2014-01-01 00:00:00