Abstract:
:Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100 nm with focused light generally required the use of two lenses in a 4Pi configuration or exceptionally bright photochromic fluorophores. Here, we describe a simple technical solution for 3D nanoscopy of fixed samples: biological specimens are fluorescently labeled, embedded in a polymer resin, cut into thin sections, and then imaged via STED microscopy with nanoscale resolution. This approach allows a 3D image reconstruction with a resolution <80 nm in all directions using available state-of-the art STED microscopes.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Punge A,Rizzoli SO,Jahn R,Wildanger JD,Meyer L,Schönle A,Kastrup L,Hell SWdoi
10.1002/jemt.20602subject
Has Abstractpub_date
2008-09-01 00:00:00pages
644-50issue
9eissn
1059-910Xissn
1097-0029journal_volume
71pub_type
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