3D reconstruction of high-resolution STED microscope images.

Abstract:

:Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100 nm with focused light generally required the use of two lenses in a 4Pi configuration or exceptionally bright photochromic fluorophores. Here, we describe a simple technical solution for 3D nanoscopy of fixed samples: biological specimens are fluorescently labeled, embedded in a polymer resin, cut into thin sections, and then imaged via STED microscopy with nanoscale resolution. This approach allows a 3D image reconstruction with a resolution <80 nm in all directions using available state-of-the art STED microscopes.

journal_name

Microsc Res Tech

authors

Punge A,Rizzoli SO,Jahn R,Wildanger JD,Meyer L,Schönle A,Kastrup L,Hell SW

doi

10.1002/jemt.20602

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

644-50

issue

9

eissn

1059-910X

issn

1097-0029

journal_volume

71

pub_type

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