Abstract:
:Progressive multifocal leukoencephalopathy is an important viral opportunistic infection of oligodendrocytes leading to direct demyelination. Virus is likely disseminated to the brain via the blood. However, the timing of that dissemination with relationship to clinical disease is unknown. Important clues about viral pathogenesis have been learned by applying molecular in situ techniques to diseased brain. The oligodendrocyte is the primary target for JC virus infection, and its death is the primary reason for demyelination. Bizarre astrocytes show limited viral DNA replication but are abortively infected. Although lymphoid organs can be infected by JC virus, there is no definitive evidence that lymphoid cells carry virus into the brain at the time of immunosuppression. JC virus may be reactivated from a latent state in both the brain and in non-central nervous system (CNS) organs at the time of immunosuppression, leading to clinical disease. Future sensitive in situ studies will likely resolve controversies about pathogenesis.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Aksamit AJ Jrdoi
10.1002/jemt.1070320405subject
Has Abstractpub_date
1995-11-01 00:00:00pages
302-11issue
4eissn
1059-910Xissn
1097-0029journal_volume
32pub_type
杂志文章,评审abstract::All organisms exhibit significant daily rhythms in a myriad of functions from molecular levels to the level of the whole organism. Significantly, most of these rhythms will persist under constant conditions, showing that they are driven by an internal circadian clock. In birds the circadian system is composed of sever...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1068
更新日期:2001-04-01 00:00:00
abstract::Various deconvolution algorithms are often used for restoration of digital images. Image deconvolution is especially needed for the correction of three-dimensional images obtained by confocal laser scanning microscopy. Such images suffer from distortions, particularly in the Z dimension. As a result, reliable automati...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20773
更新日期:2010-03-01 00:00:00
abstract::The subcommissural organ (SCO) is a brain circumventricular organ formed by ependymal and hypendymal secretory cells. It secretes glycoproteins into the cerebrospinal fluid of the third ventricle where they condense into a thread-like structure known as Reissner's fiber (RF). The present study was designed to investig...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/1097-0029(20010301)52:5<496::AID-JEMT1035>
更新日期:2001-03-01 00:00:00
abstract::The new stereology permits the objective, quantitative description of morphology by efficient and design-based methods. Applications to placentas in normal and abnormal pregnancies have proved of great value for challenging earlier misconceptions and interpreting better the processes of growth, morphogenesis, adaptati...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970701/15)38:1/2<195::AI
更新日期:1997-07-01 00:00:00
abstract::The avian pancreas has three or four lobes and develops from a dorsal and two ventral buds. The cells that will contribute to formation of the dorsal bud are at first located in the mid-dorsal endoderm, those of the ventral buds in the floor of the foregut. The determination of endoderm to form dorsal and ventral bud ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19981115)43:4<292::AID-JEM
更新日期:1998-11-15 00:00:00
abstract::The effects of crystal and beam tilt on high-resolution transmission electron microscope (HRTEM) images of planar coherent interfaces were investigated by multislice image simulations. It was found that a beam tilt of 0.5 Bragg angle (theta B) was sufficient to introduce detrimental artifacts into most images of inter...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070230306
更新日期:1992-11-01 00:00:00
abstract::The wavelength of Raman-scattered light depends on the molecular composition of the substance. This is the first attempt to acquire Raman spectra of a mouse eyeball removed from a living mouse, in which the eyeball was preserved using the "in vivo cryotechnique" followed by freeze-drying. Eyeballs were cryofixed using...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20449
更新日期:2007-07-01 00:00:00
abstract::This study was performed to observe microstructures of the rat lung, using a synchrotron radiation beam and to compare findings with histological observations. X-ray refraction images from ex-vivo ventilating rat lung were obtained with an 8 KeV monochromatic beam and 20-mum thick CsI(Tl) scintillation crystal. The vi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20335
更新日期:2006-08-01 00:00:00
abstract::We present a semiautomated software solution to the problem of extending the lateral field of view of a classical microscope. The initial requirements are a set of overlapping images, along with their user-provided coarse mosaic. Our solution then refines this initial mosaic in a fully automatic fashion. We rely on a ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20393
更新日期:2007-02-01 00:00:00
abstract::Uranium-contaminated soils from the U.S. Department of Energy (DOE) Fernald Site, Ohio, have been examined by a combination of backscattered electron imaging (BSE) and analytical electron microscopy with electron diffraction (AEM). The inhomogeneous distribution of particulate uranium phases in the soil required the d...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070310208
更新日期:1995-06-01 00:00:00
abstract::The rapid photothermal lysis of Escherichia coli O157:H7 treated with light-triggered Janus nanomotors was visualized by Hilbert differential contrast transmission electron microscopy (HDC-TEM). The extraordinary advantage of this high-resolution microscopic technique was that it revealed the detailed ultrastructure a...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23657
更新日期:2020-11-27 00:00:00
abstract::Amphibia Kupffer cells (i.e., liver resident macrophages) show many common characteristics when compared with Mammalia Kupffer cells: filopodia, microvillous-like structures, lamellipodia, fuzzy coat, coated vesicles, bristled vacuoles, nonspecific esterase activity, and pinocytotic and phagocytic activity are present...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10101
更新日期:2002-06-15 00:00:00
abstract::Filamentous bacteriophage has been proposed as a vehicle that can carry and deliver therapeutics into mammalian cells for disease treatment, thus a protocol for imaging phage-cell interaction is essential. Because high signal intensity is necessary to study the mechanism of interaction between filamentous bacteriophag...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20793
更新日期:2010-05-01 00:00:00
abstract::The tip is one of the critical factors to improve the efficiency in picking up individual DNA molecules from solid substrates based on atomic force microscope (AFM) nanomanipulation. We found that wearing AFM tips on certain solid substrates in advance to nanomanipulation operation would largely improve the pickup eff...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.21104
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND:The use of quality control tool for adulteration of Senna (Cassia aungustifolia) a pharmaceutically very important. They were used for multiple health disorders such as constipation, indigestion, epilepsy, asthma, piles, migraine, and heart problems. Two different species of same family or same genus used co...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23332
更新日期:2019-10-01 00:00:00
abstract::A major challenge when performing scanning electron microscopy and X-ray analysis on many ceramic materials is their electrical insulation properties, which leads to buildup of the surface charge and reduced contrast in the secondary electron image. A new procedure was established to quantitatively determine the neutr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23118
更新日期:2018-10-01 00:00:00
abstract::We report for the first time the presence of cluster crystals of calcium oxalate within the glandular trichomes and oil bodies in the mesophyll for Baccharis species. Moreover, the comparative leaf anatomy and micro-morphology of six species of Baccharis, namely B. illinita, B. microdonta, B. pauciflosculosa, B. punct...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23045
更新日期:2018-08-01 00:00:00
abstract::The fine structure of human oogonia and growing oocytes has been reviewed in fetal and adult ovaries. Preovulatory maturation and the ultrastructure of stimulated oocytes from the germinal vesicle (GV) stage to metaphase II (MII) stage are also documented. Oogonia have large nuclei, scanty cytoplasm with complex mitoc...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20299
更新日期:2006-06-01 00:00:00
abstract::In conventional whole-tooth culture systems, limitation exists regarding maintenance of the vitality of the dental pulp, because this tissue is encased in rigid dentin walls that hinder nutrition supply. We here report a whole tooth-in-jaw-bone culture system of rat mandibular first molars, where transcardiac perfusio...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22072
更新日期:2012-10-01 00:00:00
abstract::Breast cancer is the most common cancer diagnosed among women. In this article, support vector machine is used to classify digital mammogram images into malignant and benign. Wiener filter is used to handle the possible quantum noise, which is more likely to occur in mammograms. Stack-based connected component method ...
journal_title:Microscopy research and technique
pub_type: 信件
doi:10.1002/jemt.21070
更新日期:2011-11-01 00:00:00
abstract::Using high resolution transmission electron microscopy with image simulation and Fourier analysis, the Li1- x FePO4 (x < 0.01), Li1- x FePO4 (x ∼ 0.5), and FePO4 particles, prepared by charging or discharging the 053048 electrochemical cells (thickness: 5 mm, width: 30 mm, height: 48 mm) and dismantled inside an Ar-fi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22495
更新日期:2015-06-01 00:00:00
abstract::The possibility that tannic acid and other classical mordants can be used in the electron microscopical section staining technique has been tested. A mordant can be defined as a chemical that combines with both a certain specific tissue component and the staining substance and thereby permits a staining reaction that ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070210110
更新日期:1992-03-01 00:00:00
abstract::Frozen skin sections are routinely used for light microscopic immunohistochemical study of the skin basement membrane zone for two reasons: some skin basement membrane zone proteins are labile to routine chemical fixation, and skin is not amenable to vibratome sectioning. However, inherent limitations of conventional ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1136
更新日期:2001-08-15 00:00:00
abstract::Virus particles and viral inclusions were detected by transmission electron microscopy examination of sections of the seminal vesicles and mucus gland of asymptomatic young drones from colonies of Apis mellifera lightly infested by Varroa mite. In the mucus gland the infection was found in the muscular sheath and epit...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22024
更新日期:2012-07-01 00:00:00
abstract::Growth rings were used to determine the root age of medicinal Paeonia lactiflora from four producing areas, and their corresponding paeoniflorin content were measured based on the identification of ages. Different P. lactiflora root samples of different ages were collected from the four major growing areas in China: B...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22048
更新日期:2012-09-01 00:00:00
abstract::Nonlinear microscopy techniques are being increasingly used to perform in vivo studies in dermatology. These methods enable us to investigate the morphology and monitor the physiological process in the skin by the use of femtosecond lasers operating in the red, near-infrared spectral range (680-1,300 nm). In this work...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22545
更新日期:2015-09-01 00:00:00
abstract::This study reports the effects on micromorphology and temperature rise in human dentin using different frequencies of Er:YAG laser. Sixty human dentin fragments were randomly assigned into two groups (n = 30): carious or sound dentin. Both groups were divided into three subgroups (n = 10), according to the Er:YAG lase...
journal_title:Microscopy research and technique
pub_type: 杂志文章,随机对照试验
doi:10.1002/jemt.22076
更新日期:2012-10-01 00:00:00
abstract::This manuscript reviews gap junctions' roles in control of intestinal motility. Gap junctions (GJs) of small intestine (SmIn) are found between circular muscle (CM) cells, between interstitial cells of Cajal (ICC) of deep muscular plexus (DMP) and between them and adjacent outer circular muscle (OCM). GJs between long...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19991201)47:5<309::AID-JEM
更新日期:1999-12-01 00:00:00
abstract::This review examines the morphology of the adrenal gland with particular reference to the adrenal vasculature. It examines the possibility that variability in adrenal gland responsiveness may be attributable to neural or hormonal modulation of adrenal blood flow. Changes in the rate of blood flow through the adrenal c...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970315)36:6<546::AID-JEM
更新日期:1997-03-15 00:00:00
abstract::Classical conditioning (CC) with a sweet flavored is useful for experimental models of immune response. The objective this work was analyze spleen cell response on the differentiation of B-lymphocytes associated with the CC with sweet flavored in adult rats. Twelve adult male rats were divided (n = 3) in Group 1 (cont...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23112
更新日期:2018-12-01 00:00:00