Abstract:
:Fluorescence correlation spectroscopy (FCS) has become an increasingly important measurement tool for biological and biomedical investigations, with the capability to assay molecular dynamics and interactions both in vitro and within living cells. Information recovery in FCS requires an accurate characterization and calibration of the observation volume. A number of recent reports have demonstrated that the calibration of the observation volume is excitation power dependent, a complication that arises due to excitation saturation. While quantitative models are now available to account for these volume variations, many researchers attempt to avoid saturation issues by working with low nonsaturating excitation intensities. For two-photon excited fluorescence, this is typically thought to be achievable by working with excitation powers for which the total measured fluorescence signal maintains its quadratic dependence on excitation intensity. We demonstrate that observing only the power dependence of the fluorescence intensity will tend to underestimate the importance of saturation, and explain these findings in terms of basic physical models.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Wu J,Berland Kdoi
10.1002/jemt.20454subject
Has Abstractpub_date
2007-08-01 00:00:00pages
682-6issue
8eissn
1059-910Xissn
1097-0029journal_volume
70pub_type
杂志文章abstract::Around 30 pollen samples, 16 families and 25 genera that were gathered, identified and preserved from 6 different localities of District Sheikhupura. Light and scanning electron microscopy was done for all collected flora. Out of the total samples, 43% specie was identified as herbs, 23% as shrubs, and 33% were identi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23656
更新日期:2020-12-31 00:00:00
abstract::Sequences highly similar (>95%) to the mouse mammary tumor virus (MMTV) env gene have been amplified from human DNA samples, including DNA samples from patients with breast cancer (BC) and persons who did not have BC. The sequences from human DNA were distinct from the MMTV sequences used as controls in these PCR reac...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20233
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20299
更新日期:2006-06-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 信件
doi:10.1002/jemt.21070
更新日期:2011-11-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/1097-0029(20010301)52:5<615::AID-JEMT1045>
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22871
更新日期:2017-08-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
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更新日期:2018-12-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20725
更新日期:2009-10-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20044
更新日期:2004-04-01 00:00:00
abstract::Correlative microscopy has become increasingly important for the analysis of the structure, function, and dynamics of cells. This is largely due to the result of recent advances in light-, probe-, laser- and various electron microscopy techniques that facilitate three-dimensional studies. Furthermore, the improved und...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20408
更新日期:2007-03-01 00:00:00
abstract::This present study is the first report on the detailed foliar epidermal anatomy and micromorphology of seeds of five species of Vitaceae from Pakistan using light microscopy (LM) and scanning electron microscopy (SEM). The studied species occur at various localities particularly higher altitude. Qualitative and quanti...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23102
更新日期:2019-04-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23454
更新日期:2020-06-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22315
更新日期:2014-01-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970515)37:4<271::AID-JEM
更新日期:1997-05-15 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20000901)50:5<338::AID-JEMT3>3.0
更新日期:2000-09-01 00:00:00
abstract::In the search for a new methodological approach applicable to the determination of the still poorly known primary role of boron in plant physiology, we have undertaken to appraise the potential of the SIMS method for the analytical imaging of the boron isotopes, (10)B and (11)B, at physiological concentrations in plan...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10125
更新日期:2002-07-15 00:00:00
abstract::Gymnosporia arenicola Jordaan (Celastraceae) is a shrub or small tree, which naturally occurs in coastal sand dunes of Southern Mozambique and South Africa. Its dried leaf is often used in traditional medicine for the treatment of infectious and inflammatory diseases. Hereby, we present results of studies carried out ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22565
更新日期:2015-11-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22139
更新日期:2013-01-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23345
更新日期:2019-10-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19991215)47:6<401::AID-JEM
更新日期:1999-12-15 00:00:00
abstract::The dermal denticles are among the unique morphological adaptations of sharks, which have been acquired throughout their long evolutionary process of more than 400 million years. Species-specific morphological characteristics of these structures has been applied specially as tools for functional and taxonomic (family-...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23273
更新日期:2019-08-01 00:00:00
abstract::This report describes osteoclastic bone resorption around intraosseous fixation screws in rat and pig mandibles. These screws supported distraction devices and provided for neutral fixation following the distraction period. Progressive clinical instability of bicortical screws and radiographic and histologic evidence ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10376
更新日期:2003-08-15 00:00:00
abstract::This study evaluated the effect of chitosan, zirconium oxide, and hydroxyapatite on the apatite-like forming ability, porosity, and bond-strength of calcium-aluminate cements (C). Three hundred bovine root-slices were assigned to one of five groups, according to the material: MTA, C, C + chitosan (Cchi), C + zirconium...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23678
更新日期:2020-12-22 00:00:00
abstract::Changes in the intracellular Ca2+ concentration ([Ca2+]i) within CA1 hippocampal pyramidal neurons were imaged using confocal laser scanning microscopy in conjunction with Ca(2+)-sensitive fluorescent indicators. The imaging was performed in thick hippocampal brain slices while simultaneously measuring or controlling ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070290404
更新日期:1994-11-01 00:00:00
abstract::This study compared several irrigation protocols and application systems for sealer penetration into dentinal tubules. Single-rooted-human teeth were divided into 5 experimental groups (n = 15) and a control group (n = 5), according to final irrigation protocols: standard needle irrigation (SNI); Vibringe; Vibringe + ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22944
更新日期:2017-12-01 00:00:00
abstract::In order to test the effectiveness of a new advanced time-lapse microscopy imaging and image processing and analysis system, and to do quantitative and qualitative temporal analyses of in vitro peripheral nerve regeneration, long-term time-lapse imaging of cultures of mouse dorsal root ganglia (DRGs) was performed. DR...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20075
更新日期:2004-06-15 00:00:00
abstract::Locusts are attractive model preparations for cellular investigations of neurodevelopment. In this study, we investigate the immunocytochemical localization of histamine in the developing ventral nerve cord of two locust species, Schistocerca gregaria and Locusta migratoria. Histamine is the fast neurotransmitter of p...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20980
更新日期:2011-10-01 00:00:00
abstract::A re-examination and modification of the very old oblique illumination technique has resulted in a method for contrast enhancement in microscopes, diffracted-light contrast (DLC), which provides high-contrast, high-resolution images of unstained biological material. The technique, which utilizes the diffracted light f...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990815/01)46:4/5<334::AI
更新日期:1999-08-15 00:00:00