Abstract:
:Despite the great number of studies devoted to neural stem/progenitor cell biology, the ultrastructural characteristics of these cells in vitro have not been fully studied. To determine the fine structure of hippocampal neural progenitor cells (NPCs) in culture, mouse fetal hippocampi (E18) were extracted, dissected, and cells were expanded as adherent monolayer culture. Electron microscopy revealed that NPCs had an immature phenotype, with a high nuclear/cytoplasmic ratio, small and scant organelles, underdeveloped endoplasmic reticulum, and many free ribosomes and polysomes. Our results may contribute to a better understanding of the fine structure and physiology of hippocampal NPCs in vitro.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Tsupykov Odoi
10.1002/jemt.22454subject
Has Abstractpub_date
2015-02-01 00:00:00pages
128-33issue
2eissn
1059-910Xissn
1097-0029journal_volume
78pub_type
杂志文章abstract::During late anaphase and telophase, animal cells develop a bundle of antiparallel, interdigitating microtubules between the two daughter nuclei. Recent data indicate that this structure, called the central spindle, plays an essential role during cytokinesis. Studies in Drosophila and on vertebrate cells strongly sugge...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(20000415)49:2<202::AID-JEM
更新日期:2000-04-15 00:00:00
abstract::The adaptations of the mouse uterus to pregnancy include extensive modifications of the cells and extracellular matrix of the endometrial connective tissue that surround the embryos. Around each implanted embryo this tissue redifferentiates into a transient structure called decidua, which is formed by polygonal cells ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20265
更新日期:2006-01-01 00:00:00
abstract::Changes in the intracellular Ca2+ concentration ([Ca2+]i) within CA1 hippocampal pyramidal neurons were imaged using confocal laser scanning microscopy in conjunction with Ca(2+)-sensitive fluorescent indicators. The imaging was performed in thick hippocampal brain slices while simultaneously measuring or controlling ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070290404
更新日期:1994-11-01 00:00:00
abstract::In this article, the Ni-Cu nanoparticles (NPs) in the amorphous carbon hydrogenated thin films with different copper percentage by co-deposition of RF-plasma enhanced chemical vapor deposition were prepared using acetylene gas and Ni and Cu targets. The films deposited with 5% Cu have minimum value of the average diam...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23679
更新日期:2020-12-22 00:00:00
abstract::This study investigated the relationship among heat shock protein 70 (HSP70), proliferating cell nuclear antigen (PCNA), and testicular apoptosis during a breeding cycle of Prochilodus argenteus, a neotropical migratory characiform fish of importance in commercial fishery from the São Francisco River basin. A total of...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22173
更新日期:2013-04-01 00:00:00
abstract::The present study provides experimental data which indicate that the neutrophil is ideal for studying programmed cell death or apoptosis in vitro. Neutrophils can be obtained from human peripheral blood in large numbers with minimal experimental manipulation and are easily separated from other leukocytes, providing ne...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070280408
更新日期:1994-07-01 00:00:00
abstract::The research was performed to investigate pollen morphology of endangered species of Capparidaceae in subtropical regions of Pakistan. The distinguishing characters were investigated by using light microscope and scanning electron microscope. Palynological study is comprised of pollen shape, pollen type, exine sculptu...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23292
更新日期:2019-09-01 00:00:00
abstract::When 20-day-old rats are placed on a vitamin A deficient diet (VAD) for a period of 10 weeks, the seminiferous tubules are found to contain only Sertoli cells, a few residual A0, A1 spermatogonia, and preleptotene spermatocytes (PL). The type A1 spermatogonia and PL spermatocytes are arrested in their G2 phase. In VAD...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070200106
更新日期:1992-01-01 00:00:00
abstract::Adrenomedullin (AM) is a novel neuropeptide with special significance in the mammalian hypothalamo-hypophysial axis. By using an antiserum specific for human AM, we have studied the localization of AM-like immunoreactive (AMi) cell bodies and fibers in the hypothalamus and hypophysis of the amphibians Rana perezi (anu...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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更新日期:2001-08-01 00:00:00
abstract::Telocytes, new interstitial cells that have received significant attention in recent years, have been detected in many organs, including the heart. The distinction between telocytes and other interstitial cells can only be made based on their ultrastructural characterization and immunophenotypic features. In this stud...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23612
更新日期:2020-10-05 00:00:00
abstract::Transfer of a fluorescently labeled protein particle from a surface to a microsized scanning probe has been induced by repetitive scanning in aqueous medium. The so-attached particle can in turn act as a probing tool to study particle-substrate and particle-particle interactions. Attachment of the fluorescent particle...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20816
更新日期:2010-08-01 00:00:00
abstract::The avian pancreas has three or four lobes and develops from a dorsal and two ventral buds. The cells that will contribute to formation of the dorsal bud are at first located in the mid-dorsal endoderm, those of the ventral buds in the floor of the foregut. The determination of endoderm to form dorsal and ventral bud ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19981115)43:4<292::AID-JEM
更新日期:1998-11-15 00:00:00
abstract:OBJECTIVES:This study investigated the effects of sliding on the ultrastructure of three representative esthetic superelastic 0.014 inch nickel-titanium (NiTi) archwires. METHODS:Atomic force microscopy, scanning electron microscopy, and light microscopy were used to estimate the surface roughness of archwires and bra...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22557
更新日期:2015-10-01 00:00:00
abstract::Growth-year authentication has extraordinary significance for plant growth, structure and development research, and has a wide range of applications in value assessment of economic crops. Panax ginseng is the most commonly used medicinal plant in Asian countries. The fix number of growth-year is an important quality e...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22610
更新日期:2016-02-01 00:00:00
abstract::The family Scrophulariaceae consists of taxonomically complex genera and species. The delimitation of the taxa within this family is always challenging. In this paper, we studied leaf epidermis anatomical characteristics and its taxonomic significance of four species belonging to four genera of the family Scrophularia...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23608
更新日期:2020-09-29 00:00:00
abstract::Several studies searching for methods to control Rhipicephalus sanguineus s.l., (dog tick) infestations have been developed aiming to minimize the damages caused by these ectoparasites to the hosts and the environment, which is harmed by the indiscriminate use of toxic acaricide products. In this scenario, neem oil ha...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22871
更新日期:2017-08-01 00:00:00
abstract::Different methods have been established for the simultaneous detection of different pathogens in tissue samples, each with certain advantages and disadvantages. Chromogenic in situ hybridization combines specific molecular pathogen detection with microscopic evaluation of pathogen quantity, morphology and distribution...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20546
更新日期:2008-04-01 00:00:00
abstract::Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100 nm with focu...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20602
更新日期:2008-09-01 00:00:00
abstract::Förster resonance energy transfer (FRET) allows one to study interactions between two fluorescently labeled molecules (donors and acceptors) at distances on the order of 5 nm. Many studies have described methods of how to measure the efficiency of FRET. However, few have addressed the question of how fluorescence from...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20078
更新日期:2004-06-01 00:00:00
abstract::Bats are the only mammals that can fly in the dark without eye usage. This study was conducted to describe the structural and functional adaptations of the retina of two bats very common in the Egyptian fauna having a different lifestyle: the Egyptian fruit bat (Rousettus aegyptiacus) and insectivorous bat (Pipistrell...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23530
更新日期:2020-11-01 00:00:00
abstract::In order to fulfill its many functions as the selective interface between maternal and fetal circulations it is imperative that the human placenta remains intact and in good operational order. That damage of some sort occurs during its short but extremely active life seems inevitable given the dynamic environment in w...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980901)42:5<351::AID-JEM
更新日期:1998-09-01 00:00:00
abstract::This work provides examples of some of the imaging capabilities of environmental scanning electron microscopy applied to easily charged samples relevant to particle analysis. Environmental SEM (also referred to as high pressure or low vacuum SEM) can address uncoated samples that are known to be difficult to image. Mo...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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更新日期:1993-08-01 00:00:00
abstract::The discovery of new song types in Hawaiian Drosophila species has raised a question of alternative sound production and sound perception mechanisms in Drosophila. For example D. disjuncta males, which produce song with a carrier frequency of 5,000-6,000 Hz, do not vibrate their wings but only tremble them in a very s...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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更新日期:2000-07-15 00:00:00
abstract::Frozen skin sections are routinely used for light microscopic immunohistochemical study of the skin basement membrane zone for two reasons: some skin basement membrane zone proteins are labile to routine chemical fixation, and skin is not amenable to vibratome sectioning. However, inherent limitations of conventional ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1136
更新日期:2001-08-15 00:00:00
abstract::The effectiveness of different microscopy techniques for measuring the dimensions of ultimate fibers from harakeke (Phormium tenax, New Zealand flax) was investigated using a factorial experimental design. Constant variables were geographical location, location of specimens along the leaf, season (winter), individual ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20206
更新日期:2005-08-01 00:00:00
abstract::An understanding of the internal morphology of the ant mandible is important in explaining the relationship between the exocrine system and the behavioral and phylogenetic characteristics of different subfamilies of Formicidae. In this study, we investigated the occurrence of intramandibular glands in ants from the Po...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
abstract::We report on a novel laser source, emitting high energy (20 nanoJoule) femtosecond pulses, in a broad spectrum (250 nm). This source is easily tuned from 950 to 1200 nm, without any laser adjustment, and delivers sub-300 femtosecond pulses with a 10-nm spectral width. ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10419
更新日期:2004-01-01 00:00:00
abstract::Diestian ferruginous sandstone has been used as the dominant building stone for monuments in the Hageland, a natural landscape in east-central Belgium. Like all rocks, this stone type is sensitive to weathering. Case hardening was observed in combination with blackening of the exterior parts of the dressed stones. To ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20987
更新日期:2011-11-01 00:00:00
abstract::A photochemical reaction was used to produce focal microcirculation disorders in the guinea pig cochlea. Temporal bones were removed at various intervals between 5 minutes and 1 month after infusion of rose bengal (RB) and illumination. Infused but unilluminated contralateral cochleae served as controls. Dissected coc...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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更新日期:1998-05-15 00:00:00
abstract::The density of nodes of Ranvier was examined at CNS, PNS, and transitional zone (TZ) levels of rat lumbar ventral motoneurone fibres. It was found to be significantly greater in the TZ than at the other levels: The difference was sevenfold for the ventral root and at least fourfold for central fibre levels. Node distr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19960815)34:6<507::AID-JEM
更新日期:1996-08-15 00:00:00