Abstract:
:Changes in the intracellular Ca2+ concentration ([Ca2+]i) within CA1 hippocampal pyramidal neurons were imaged using confocal laser scanning microscopy in conjunction with Ca(2+)-sensitive fluorescent indicators. The imaging was performed in thick hippocampal brain slices while simultaneously measuring or controlling electrical activity with sharp microelectrodes or whole-cell patch-clamp electrodes. The combination of imaging and electrophysiology was essential for interpreting the changes in [Ca2+]i. We compared the increases in [Ca2+]i produced by either of two methods--direct depolarization of the cell via the somatic electrode or high-frequency stimulations of synaptic inputs. The increases in [Ca2+]i in the soma and proximal dendrites caused by both methods were of comparable magnitude and they always decayed within seconds in healthy cells. However, the spatial patterns of distal Ca2+ increases were different. Separate sets of synaptic inputs to the same cell resulted in different spatial patterns of [Ca2+]i transients. We isolated and observed what appeared to be a voltage-independent component of the synaptically mediated [Ca2+]i transients. This work demonstrates that the combination of neurophysiology and simultaneous confocal microscopy is well suited for visualizing and analyzing [Ca2+]i changes within highly localized regions of neurons in thick brain slices. The approach should allow further analysis of the relative contribution of voltage- and agonist-dependent influences on [Ca2+]i within neurons throughout the CNS and it raises the possibility of routinely relating subcellular [Ca2+]i changes to structural and functional modifications.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Jaffe DB,Brown THdoi
10.1002/jemt.1070290404subject
Has Abstractpub_date
1994-11-01 00:00:00pages
279-89issue
4eissn
1059-910Xissn
1097-0029journal_volume
29pub_type
杂志文章abstract::On continuing our studies on head and neck neoplasia, specimens from salivary gland tumors have been explored by using infrared microimaging spectroscopy to discern healthy from neoplastic tissues. Samples with Warthin tumor, epithelial displasia, marginal B-cell lymphoma, low-grade adenocarcinoma, and adenoid cystic ...
journal_title:Microscopy research and technique
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更新日期:2009-02-01 00:00:00
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
pub_type: 杂志文章
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
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abstract::Growth-year authentication has extraordinary significance for plant growth, structure and development research, and has a wide range of applications in value assessment of economic crops. Panax ginseng is the most commonly used medicinal plant in Asian countries. The fix number of growth-year is an important quality e...
journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Microscopy research and technique
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doi:10.1002/jemt.20882
更新日期:2011-02-01 00:00:00
abstract::The objectives were to develop method of isolating viable human limbal basal cells in order to enrich a subset of small cells with a large Nucleus/Cytoplasm (N/C) ratio expressing high levels of p63, nuclear protein. Limbal tissues were treated with trypsin for 50 min at 37 degrees C in an orbital shaker at 100 rpm wi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20573
更新日期:2008-06-01 00:00:00
abstract::After more than two centuries of almost sporadic inquiry as to the existence and function of the human vomeronasal system (VNS), the last decade has seen a resurgent interest in it. The principal question vexing many laboratories is whether adult humans retain the VNS that clearly develops during fetal growth. Additio...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19980615)41:6<530::AID-JEM
更新日期:1998-06-15 00:00:00
abstract::An analytical result is given for the wavelength at which two or more first order Laue zone (FOLZ) lines intersect at a single point on the positive x axis when a face centred cubic crystal is observed in the convergent beam mode with the beam axis parallel to the [100] direction. The position of the intersection alon...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070290311
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19991201)47:5<309::AID-JEM
更新日期:1999-12-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
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journal_title:Microscopy research and technique
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abstract::The human dentin-enamel junction (DEJ) is a natural junction that unites two dissimilar mineralized tissues in the human tooth: enamel and dentin. DEJ plays a critical role in maintaining structural and functional integrity of the tooth. However, its structure, chemical composition and function remain unclear and cont...
journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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abstract::The ultrastructural characteristics of peptidergic peripheral contacts in the snail, Helix pomatia, were investigated, with special attention to the innervation of the heart, buccal mass, and salivary gland by Mytilus inhibitory peptide-immunoreactive neurons. Following the application of correlative light- and electr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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abstract::Mechanically dissociated neuronal cell bodies from the thoracic ganglia of Locusta migratoria were viable in culture conditions for up to 2 days and were voltage-clamped to record the effects of GABAergic drugs and physostigmine on the membrane conductance and ACh responses of the dissociated cells. Bicuculline, hydra...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20010201)52:3<273::AID-JEMT1013>
更新日期:2001-02-01 00:00:00
abstract::Using high resolution transmission electron microscopy with image simulation and Fourier analysis, the Li1- x FePO4 (x < 0.01), Li1- x FePO4 (x ∼ 0.5), and FePO4 particles, prepared by charging or discharging the 053048 electrochemical cells (thickness: 5 mm, width: 30 mm, height: 48 mm) and dismantled inside an Ar-fi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00
abstract::This experiment was conducted in Ile-de-France adult rams to examine the target point of a 2-month light cycle regimen on seminiferous tubule functions, on intertubular compartment and on Leydig cell parameters. Eight rams were subjected to a 2-month light cycle regimen and were compared to sexually active or inactive...
journal_title:Microscopy research and technique
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doi:10.1002/jemt.1070200306
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abstract::The present study focused to elucidate the promising role of probiotics in the fish culture, by employing microscopic and biochemical parameters to rationalize the possible outcomes. After preliminary feeding trial histopathological analysis revealed clear and visible changes in the intestinal and liver cells of CTRL ...
journal_title:Microscopy research and technique
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abstract::The carotid body (CB) is a highly specialized small organ located at the bifurcation of the common carotid artery in the neck and plays an important role in acute adaptation to hypoxia. The most common diseased state of the carotid body is its enlargement (i.e., the CB paraganglioma), which can be caused by a genetic ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10200
更新日期:2002-11-01 00:00:00
abstract::The implantation of breast prostheses for both aesthetic and reconstructive purposes has been growing exponentially in the last 20 years. Safety and prosthesis lifespan are majorly debated issues in relation to the correlated long-term complications. Mainly the periprosthetic capsule that develops around the implant i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23673
更新日期:2020-12-10 00:00:00
abstract::Atomic force microscope (AFM) has been widely used in the biological field owing to its high sensitivity (subnanonewton), high spatial resolution (nanometer), and adaptability to physiological environments. Nowadays, force volume (FV) and peakforce quantitative nanomechanical (QNM) are two distinct modes of AFM used i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23351
更新日期:2019-11-01 00:00:00