Abstract:
:This work provides examples of some of the imaging capabilities of environmental scanning electron microscopy applied to easily charged samples relevant to particle analysis. Environmental SEM (also referred to as high pressure or low vacuum SEM) can address uncoated samples that are known to be difficult to image. Most of these specimens are difficult to image by conventional SEM even when coated with a conductive layer. Another area where environmental SEM is particularly applicable is for specimens not compatible with high vacuum, such as volatile specimens. Samples from which images were obtained that otherwise may not have been possible by conventional methods included fly ash particles on an oiled plastic membrane impactor substrate, a one micrometer diameter fiber mounted on the end of a wire, uranium oxide particles embedded in oil-bearing cellulose nitrate, teflon and polycarbonate filter materials with collected air particulate matter, polystyrene latex spheres on cellulosic filter paper, polystyrene latex spheres "loosely" sitting on a glass slide, and subsurface tracks in an etched nuclear track-etch detector. Surface charging problems experienced in high vacuum SEMs are virtually eliminated in the low vacuum SEM, extending imaging capabilities to samples previously difficult to use or incompatible with conventional methods.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Wight SA,Zeissler CJdoi
10.1002/jemt.1070250507subject
Has Abstractpub_date
1993-08-01 00:00:00pages
393-7issue
5-6eissn
1059-910Xissn
1097-0029journal_volume
25pub_type
杂志文章abstract::Regeneration of skeletal parts of fossil echinoderms is reviewed. Among regenerations of various fossil echinoderms, those of crinoids are most common and many examples have been reported. Mesozoic and Cenozoic crinoids, the subclass Articulata, are characterized by the dominance of muscular articulations in the arms ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1186
更新日期:2001-12-15 00:00:00
abstract::Locusts are attractive model preparations for cellular investigations of neurodevelopment. In this study, we investigate the immunocytochemical localization of histamine in the developing ventral nerve cord of two locust species, Schistocerca gregaria and Locusta migratoria. Histamine is the fast neurotransmitter of p...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20980
更新日期:2011-10-01 00:00:00
abstract::Sequences highly similar (>95%) to the mouse mammary tumor virus (MMTV) env gene have been amplified from human DNA samples, including DNA samples from patients with breast cancer (BC) and persons who did not have BC. The sequences from human DNA were distinct from the MMTV sequences used as controls in these PCR reac...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20233
更新日期:2005-11-01 00:00:00
abstract:BACKGROUND:Ultrasonic excitation (US) was applied to glass ionomer cement (GIC) during early set time to increase the advantageous properties of this material. PURPOSE:The aim of this in vitro study was to assess the inner porosity of GIC after US. STUDY DESIGN:A total of 16 specimens, for each material, were prepare...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20873
更新日期:2011-01-01 00:00:00
abstract::A synchrotron radiation fiber diffraction structural study of the axial filament of siliceous spicules from two species of marine sponges (the Demosponge Geodia cydonium and the Hexactinellid Scolymastra joubini) was carried out. The sharpness of the spots in the diffraction patterns indicated that the protein units i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10403
更新日期:2003-11-01 00:00:00
abstract::The catecholaminergic cell groups in the human brain, denominated from A1 to A17, display some striking anatomical differences with those described in the rodent. These differences are essentially observed in the extent of the dopaminergic neurons and especially their axonal fields in the telencephalon. Immunocytochem...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990701)46:1<24::AID-JEMT
更新日期:1999-07-01 00:00:00
abstract::In order to understand the interaction between dendritic cells (DCs) and helper T (Th) cells in the region exposed to antigens during pulmonary delayed-type hypersensitivity (DTH), which is considered to be mediated by Th1 cells, we immunohistochemically investigated their spatial relationship in the cellular infiltra...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1091
更新日期:2001-05-15 00:00:00
abstract::Low voltage electron microscopes working in transmission mode, like LVEM5 (Delong Instruments, Czech Republic) working at accelerating voltage 5 kV or scanning electron microscope working in transmission mode with accelerating voltage below 1 kV, require ultrathin sections with the thickness below 20 nm. Decreasing of...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22659
更新日期:2016-06-01 00:00:00
abstract::A photochemical reaction was used to produce focal microcirculation disorders in the guinea pig cochlea. Temporal bones were removed at various intervals between 5 minutes and 1 month after infusion of rose bengal (RB) and illumination. Infused but unilluminated contralateral cochleae served as controls. Dissected coc...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980515)41:4<323::AID-JEM
更新日期:1998-05-15 00:00:00
abstract::The importance of chloride channels for the cell is demonstrated by a number of serious human diseases that are due to mutations in chloride channels. The most well-known of these diseases is cystic fibrosis. Investigations into the mechanisms of the disease and possible treatments require the study of chloride fluxes...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10234
更新日期:2002-12-15 00:00:00
abstract::Effective protocols for controlling crystal structure, size, and morphology attract considerable interest given the requirement for particles of modal size and shape in many areas of materials fabrication and the importance of crystallochemical selectivity in determining the exploitable properties of inorganic solids....
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070270504
更新日期:1994-04-01 00:00:00
abstract::Uranium-contaminated soils from the U.S. Department of Energy (DOE) Fernald Site, Ohio, have been examined by a combination of backscattered electron imaging (BSE) and analytical electron microscopy with electron diffraction (AEM). The inhomogeneous distribution of particulate uranium phases in the soil required the d...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070310208
更新日期:1995-06-01 00:00:00
abstract::Nonlinear microscopy techniques are being increasingly used to perform in vivo studies in dermatology. These methods enable us to investigate the morphology and monitor the physiological process in the skin by the use of femtosecond lasers operating in the red, near-infrared spectral range (680-1,300 nm). In this work...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22545
更新日期:2015-09-01 00:00:00
abstract::Understanding the functional morphology and mobility of appendages of fossil animals is important for exploring ecological traits such as feeding and locomotion. Previous work on fossils from the 518 million-year-old Chengjiang biota of China was based mainly on two-dimensional information captured from the surface of...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23628
更新日期:2020-11-06 00:00:00
abstract::The fine structure of the taste buds of circumvallate papillae of two strains of mice was studied by electron microscopy. Mice anesthetized with ketamine were perfused through the heart with a double aldehyde mixture in cacodylate buffer and the tissues embedded in Epon. Semi-serial sections were employed. The morphol...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070260304
更新日期:1993-10-15 00:00:00
abstract::The morphology of pancreatic excretory duct segments was reviewed in mammals. The fine structure of the epithelial lining was described in intercalated ducts, intra- and extralobular ducts, and in major pancreatic ducts. Morphological characteristics of the various cell types comprising to the duct epithelium were det...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970601)37:5/6<407::AID-J
更新日期:1997-06-01 00:00:00
abstract::Pituitary-derived factors, including ACTH, have been widely implicated in initiating adrenal regeneration. However, recent work has demonstrated that adrenal regeneration is also modulated by adrenal nerves that extensively reinnervate the regenerating adrenal. Moreover, transection of the splanchnic nerve removes sen...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10334
更新日期:2003-06-15 00:00:00
abstract::This article reviews recent research using a low-energy electron microscope, built by Tromp at IBM, and equipped with an accelerator that permits in situ irradiation with a beam of self-ions. The available ion energies of 20 eV to 5 keV span the range from epitaxial growth by a hyperthermal beam to sputtering at the l...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20672
更新日期:2009-03-01 00:00:00
abstract::All organisms exhibit significant daily rhythms in a myriad of functions from molecular levels to the level of the whole organism. Significantly, most of these rhythms will persist under constant conditions, showing that they are driven by an internal circadian clock. In birds the circadian system is composed of sever...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1068
更新日期:2001-04-01 00:00:00
abstract::We have used our opossum model of fetal nephrotoxicity to investigate uranyl nitrate-induced morphologic changes in the developing kidney. The present study establishes a renal dose response curve for the uranyl nitrate (UN). We find that pups treated with nonlethal doses of UN do not demonstrate growth retardation co...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19971101)39:3<285::AID-JEM
更新日期:1997-11-01 00:00:00
abstract::When 20-day-old rats are placed on a vitamin A deficient diet (VAD) for a period of 10 weeks, the seminiferous tubules are found to contain only Sertoli cells, a few residual A0, A1 spermatogonia, and preleptotene spermatocytes (PL). The type A1 spermatogonia and PL spermatocytes are arrested in their G2 phase. In VAD...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070200106
更新日期:1992-01-01 00:00:00
abstract::In spite of accumulating evidence for a modulation of sympathetic neurotransmission by endogenously produced nitric oxide (NO), it remains unclear in which parts of the vascular system and at what level this interaction takes place. The aim of the present study was to investigate the distribution of endothelial and ne...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070290214
更新日期:1994-10-01 00:00:00
abstract::Myotonic dystrophy (DM) is an inherited multisystem disorder characterized by the presence of a high polymorphic expansion of trinucleotide (CTG) repeat in the 3' untranslated region of the DM protein kinase (DMPK) gene. However, the role of myotonic dystrophy protein kinase (DMPK) has yet to be elucidated. Studies ai...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10223
更新日期:2002-09-01 00:00:00
abstract::The opossum Monodelphis domestica presents movement of the temporomandibular joint (TMJ) reflecting adaptation to eating habits similar to movement in humans, but the structure of the TMJ is not yet known. Thus, nine young M. domestica, of both sexes were weighed, anesthetized with xylazine (10 mg kg(-1) ), and ketami...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22702
更新日期:2016-09-01 00:00:00
abstract::Microscopic imaging of uneven surfaces is difficult because of the limited depth of field. In this study, we developed a rapid auto-focus method for uneven surfaces based on image fusion. The Prewitt operator was used to detect the vertical edges of the images. Then, the focus position was theoretically calculated usi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23328
更新日期:2019-09-01 00:00:00
abstract::Cell-free extracts of Xenopus eggs were used to investigate how differing lamin sub-types are incorporated into a lamina and how lamina composition influences DNA replication. Purified recombinant human lamin A (HlaminA) was inoculated into egg extracts, which support in vitro nuclear assembly. The route of incorporat...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990401)45:1<1::AID-JEMT1
更新日期:1999-04-01 00:00:00
abstract::Brain tumor identification using magnetic resonance images (MRI) is an important research domain in the field of medical imaging. Use of computerized techniques helps the doctors for the diagnosis and treatment against brain cancer. In this article, an automated system is developed for tumor extraction and classificat...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23238
更新日期:2019-06-01 00:00:00
abstract::The principal functions of the gastrointestinal tract mucosa include nutrient absorption, protein and fluid secretion, and the regulated symbiosis with intraluminal contents. Research in epithelial biology has benefited significantly from the use of cultured monolayer preparations, which closely replicate the structur...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/1097-0029(20001015)51:2<204::AID-JEMT11>3.
更新日期:2000-10-15 00:00:00
abstract::In yeast, peroxisomes are the site of specific catabolic pathways that characteristically include hydrogen peroxide producing oxidases and catalase. During the last 10 years, much progress has been made in unravelling the molecular mechanisms involved in the biogenesis of this organelle. At present, 23 different genes...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20001215)51:6<584::AID-JEMT8>3.0
更新日期:2000-12-15 00:00:00
abstract::Acrylic resin mixtures are commonly used to study microscopic sections of biological specimens, giving the advantage of good morphological preservation. Existing embedding protocols, however, are suitable for tissue blocks, not exceeding 1 mm in thickness. We have developed a protocol to embed larger specimens (up to ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20139
更新日期:2005-01-01 00:00:00