Fiber diffraction study of spicules from marine sponges.

Abstract:

:A synchrotron radiation fiber diffraction structural study of the axial filament of siliceous spicules from two species of marine sponges (the Demosponge Geodia cydonium and the Hexactinellid Scolymastra joubini) was carried out. The sharpness of the spots in the diffraction patterns indicated that the protein units in the filament of both samples were highly organized. A possible explanation is that the arrangement of the protein units is similar to that of the pores in highly ordered siliceous mesoporous materials. Nevertheless, the diffraction patterns are quite different for the two types of spicules. The pattern of G. cydonium is consistent with a regular 2D hexagonal lattice of protein units in the direction perpendicular to the spicule axis, with a repeating distance of 5.8 nm; the units are linked to form fibers along the axis. The pattern of S. joubini indicates the presence of two different 2D lattices in which the repeating protein units are inclined by +50 degrees and -50 degrees with respect to the elongation axis; the distance between the units increases to 8.4 nm. This 2D model is consistent with hexagonal packing of spirally oriented cylindrical protein units elongated along the filament axis.

journal_name

Microsc Res Tech

authors

Croce G,Frache A,Milanesio M,Viterbo D,Bavestrello G,Benatti U,Giovine M,Amenitsch H

doi

10.1002/jemt.10403

keywords:

subject

Has Abstract

pub_date

2003-11-01 00:00:00

pages

378-81

issue

4

eissn

1059-910X

issn

1097-0029

journal_volume

62

pub_type

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