Abstract:
:Acrylic resin mixtures are commonly used to study microscopic sections of biological specimens, giving the advantage of good morphological preservation. Existing embedding protocols, however, are suitable for tissue blocks, not exceeding 1 mm in thickness. We have developed a protocol to embed larger specimens (up to 2 cm(3)) in Technovit 8100. This medium allowed us to perform classic histological (trichrome), silver, as well as immunohistochemical staining, needed for multi-signal detection at high-resolution imaging to reconstruct a three-dimensional interpretation of a serially sectioned muscle. The technique was applied to reconstruct the semitendinosus muscle of a fetal pig, 44 days post conception, featuring connective tissue, intramuscular nerves, blood vessels, and muscle fibre types. For the reconstruction, a technique was used that enabled us to insert high-resolution images of histological details into low-resolution images of the entire muscle.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
De Jonge HW,De Bakker MA,Verbeek FJ,Weijs WAdoi
10.1002/jemt.20139keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
25-30issue
1eissn
1059-910Xissn
1097-0029journal_volume
66pub_type
杂志文章abstract::Detailed maps of the A-T distribution within human mitotic chromosomes labeled with BrdU are obtained with a high resolution scanning ion microprobe through the detection of bromine by imaging secondary ion mass spectrometry (SIMS). Corresponding maps of the emission loci of the molecular ion CN- describe the overall ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19970215)36:4<301::AID-JEM
更新日期:1997-02-15 00:00:00
abstract::This study was performed to observe microstructures of the rat lung, using a synchrotron radiation beam and to compare findings with histological observations. X-ray refraction images from ex-vivo ventilating rat lung were obtained with an 8 KeV monochromatic beam and 20-mum thick CsI(Tl) scintillation crystal. The vi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20335
更新日期:2006-08-01 00:00:00
abstract::Insects rely heavily on olfaction to locate habitat, mates, and oviposition sites, while odorant molecules and the antennal olfactory sensory cells of insects are two indispensable components of olfactory response. Our previous work identified the sex pheromones and volatile compounds derived from host plant of Dendro...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22134
更新日期:2013-01-01 00:00:00
abstract::In this article, the Ni-Cu nanoparticles (NPs) in the amorphous carbon hydrogenated thin films with different copper percentage by co-deposition of RF-plasma enhanced chemical vapor deposition were prepared using acetylene gas and Ni and Cu targets. The films deposited with 5% Cu have minimum value of the average diam...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23679
更新日期:2020-12-22 00:00:00
abstract::Classical conditioning (CC) with a sweet flavored is useful for experimental models of immune response. The objective this work was analyze spleen cell response on the differentiation of B-lymphocytes associated with the CC with sweet flavored in adult rats. Twelve adult male rats were divided (n = 3) in Group 1 (cont...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23112
更新日期:2018-12-01 00:00:00
abstract::This study was carried out to identify 20 high valued medicinal plants of District Lahore, Punjab, Pakistan with the help of the palynological studies. The distinguishing characteristics for selected species were found by systematized methods using light microscopy and Scanning Electron Microscopy. In this study, impo...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23096
更新日期:2018-12-01 00:00:00
abstract::During late anaphase and telophase, animal cells develop a bundle of antiparallel, interdigitating microtubules between the two daughter nuclei. Recent data indicate that this structure, called the central spindle, plays an essential role during cytokinesis. Studies in Drosophila and on vertebrate cells strongly sugge...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(20000415)49:2<202::AID-JEM
更新日期:2000-04-15 00:00:00
abstract::The dynamic transmission electron microscope (DTEM) is introduced as a novel tool for in situ processing of materials. Examples of various types of dynamic studies outline the advantages and differences of laser-based heating in the DTEM in comparison to conventional (resistive) heating in situ TEM methods. We demonst...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20664
更新日期:2009-03-01 00:00:00
abstract::The rapid photothermal lysis of Escherichia coli O157:H7 treated with light-triggered Janus nanomotors was visualized by Hilbert differential contrast transmission electron microscopy (HDC-TEM). The extraordinary advantage of this high-resolution microscopic technique was that it revealed the detailed ultrastructure a...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23657
更新日期:2020-11-27 00:00:00
abstract::Polycrystalline silver catalysts are used extensively for the partial oxidation of methanol to formaldehyde, which is then primarily incorporated in the synthesis process for adhesives and resins. In order to maximize formaldehyde production it is essential to gain a comprehensive understanding of the complex microstr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19970301)36:5<382::AID-JEM
更新日期:1997-03-01 00:00:00
abstract::Growth-year authentication has extraordinary significance for plant growth, structure and development research, and has a wide range of applications in value assessment of economic crops. Panax ginseng is the most commonly used medicinal plant in Asian countries. The fix number of growth-year is an important quality e...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22610
更新日期:2016-02-01 00:00:00
abstract:OBJECTIVES:To examine different types of restorative materials used in children as well as primary and permanent teeth enamel when affected by erosive foods. MATERIALS AND METHOD:Buttermilk, fruit yoghurt, Coca-cola, fruit juice, Filtek Z-250, Dyract Extra, Fuji II LC, and Fuji IX and tooth enamel were used. Measureme...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22315
更新日期:2014-01-01 00:00:00
abstract::Phytoliths are an important component for interpreting the ancient botanical record. However, phytoliths can be altered through heating, either as the result of such activities as firing ceramics, clay molds use for casting metal or in hearths. Phytoliths can also be altered through heating as the result of creating c...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22004
更新日期:2012-07-01 00:00:00
abstract::This study reports the effects on micromorphology and temperature rise in human dentin using different frequencies of Er:YAG laser. Sixty human dentin fragments were randomly assigned into two groups (n = 30): carious or sound dentin. Both groups were divided into three subgroups (n = 10), according to the Er:YAG lase...
journal_title:Microscopy research and technique
pub_type: 杂志文章,随机对照试验
doi:10.1002/jemt.22076
更新日期:2012-10-01 00:00:00
abstract::Historically, there has been much debate on the nature of infantile hemangiomas as either congenital malformations or benign neoplasms. Some vascular lesions that are present at birth and evidence no proliferative growth are considered to be congenital malformations; other post-natal vascular tumors pursue aggressive ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10254
更新日期:2003-02-01 00:00:00
abstract::Focused ion beam (FIB) techniques are among the most important tools for the nanostructuring of surfaces. We used the FIB/SEM (scanning electron microscope) for milling and imaging of digestive gland cells. The aim of our study was to document the interactions of FIB with the surface of the biological sample during FI...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20494
更新日期:2007-10-01 00:00:00
abstract::Lungfish are a group of ancient fish, represented almost continuously in the fossil record from their first appearance in the Devonian to the present time. They have numerous unique characters in the dentition, found only among lungfish, as well as some that are shared with other groups of fish and with higher vertebr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10221
更新日期:2002-12-01 00:00:00
abstract::It is known that, besides a wide range of functions, melatonin provides protection against oxidative stress, thanks to its ability to act, directly, as a free radical scavenger and, indirectly, by stimulating antioxidant enzymes production and mitochondrial electron transport chain efficiency. Oxidative stress is one ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22663
更新日期:2016-06-01 00:00:00
abstract::Gap junctions are specialized plasma membrane structures that join neighboring cells via specialized intercellular ion channels (hemichannels) and provide a direct pathway for cell-cell communication. They presumably mediate regulation of growth, transmission of developmental signals, coordination of muscle contractio...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20010201)52:3<273::AID-JEMT1013>
更新日期:2001-02-01 00:00:00
abstract::The Neotropical catfish, Corydoras paleatus (Callichthyidae) is a facultative air-breathing teleost that makes use of the caudal portion of the intestine as an accessory air-breathing organ. This portion is highly modified, being well vascularized with capillaries between epithelial cells, which makes it well suited f...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22614
更新日期:2016-03-01 00:00:00
abstract::We have summarized current knowledge on the aging of the immune system in three vertebrate groups: fish, amphibians and birds. Available data are few due to difficulties in studying ageing in natural populations and in accurately determining age. In all vertebrates, the most obvious evidence of the senescence of lymph...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10409
更新日期:2003-12-15 00:00:00
abstract::Morphological studies of the microcirculatory system in the thymus were reviewed in regards to methodology and structural organization of blood and lymphatic vessels. The blood capillaries and postcapillary venules (PCVs) in the thymus are characterized by a double-walled structure. These vessels are surrounded more o...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19970801)38:3<287::AID-JEM
更新日期:1997-08-01 00:00:00
abstract::In order to test the effectiveness of a new advanced time-lapse microscopy imaging and image processing and analysis system, and to do quantitative and qualitative temporal analyses of in vitro peripheral nerve regeneration, long-term time-lapse imaging of cultures of mouse dorsal root ganglia (DRGs) was performed. DR...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20075
更新日期:2004-06-15 00:00:00
abstract::Atomic force microscopy has been used to study self-assembled structures of two alpha-hemolysin mutants. For a mutant (alphaHL-H5) that was locked into the prepore state on fluid phase egg-PC membranes, we visualized, for the first time, heptameric prepores and showed that the 7-fold axis in the prepore lies perpendic...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990301)44:5<353::AID-JEM
更新日期:1999-03-01 00:00:00
abstract::Common mulberry weed (Fatoua villosa (Thunb.) Nakai) and creeping amaranth (Amaranthus crassipes Schlecht) are reported for the first time in Pakistan's flora as these were not listed in any other literature nor identified before in Pakistan. Plants were found as a result of taxonomic studies performed in 2013 in Pesh...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23000
更新日期:2018-05-01 00:00:00
abstract::In this study we report for the first time the localization of a photoreceptor pigment in the cilia of the colored heterotrich ciliates Blepharisma japonicum red and blue form, Fabrea salina, and Stentor coeruleus, as result of a confocal microscopy investigation. Optical sectioning confocal microscopy has been used f...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20508
更新日期:2007-12-01 00:00:00
abstract:BACKGROUND:Splint immobilization of the forearm is often performed in clinical practice. Previous studies investigated the effect of immobilization on bone, cartilage, muscle, and tendon, however, the acute effects on human skin microcirculation and histomorphology remains elusive. METHODS:In 12 healthy, nonsmoking in...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22317
更新日期:2014-01-01 00:00:00
abstract::We report on a novel laser source, emitting high energy (20 nanoJoule) femtosecond pulses, in a broad spectrum (250 nm). This source is easily tuned from 950 to 1200 nm, without any laser adjustment, and delivers sub-300 femtosecond pulses with a 10-nm spectral width. ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10419
更新日期:2004-01-01 00:00:00
abstract::An energy-filtering transmission electron microscope with 300 kV acceleration voltage was developed and the spatial resolution of elemental distribution images was improved. Observing oxygen monolayers in Al(11)O(3)N(9), it was shown that the actual resolution attained is up to 0.5 nm. Surface plasmon loss images of s...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20025
更新日期:2004-02-15 00:00:00
abstract::The purpose of this study was to evaluate mineral content of root canal dentin after treatment with different antibiotic pastes including the mixture of metronidazole, ciprofloxacin, doxycycline, cefaclor, amoxicillin, or minocycline. Fifty extracted maxillary canine teeth were randomly divided into five groups (n = 1...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23155
更新日期:2019-02-01 00:00:00