Abstract:
:Nonlinear microscopy techniques are being increasingly used to perform in vivo studies in dermatology. These methods enable us to investigate the morphology and monitor the physiological process in the skin by the use of femtosecond lasers operating in the red, near-infrared spectral range (680-1,300 nm). In this work we used two different techniques that require no labeling: second harmonic generation (SHG) for collagen detection and coherent anti-Stokes Raman scattering (CARS) to assess lipid distribution in genetically obese murine skin. Obesity is one of the most serious public health problems due to its high and increasing prevalence and the associated risk of type 2 diabetes and cardiovascular diseases. Other than these diseases, nearly half of patients with diabetes mellitus suffer from dermatological complications such as delayed wound healing, foot ulcers and several other skin changes. In our experiment we investigated and followed the effects of obesity on dermal collagen alterations and adipocyte enlargement using a technique not reported in the literature so far. Our results indicate that the in vivo SHG and ex vivo CARS imaging technique might be an important tool for diagnosis of diabetes-related skin disorders in the near future.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Haluszka D,Lőrincz K,Molnár G,Tamás G,Kolonics A,Szipőcs R,Kárpáti S,Wikonkál NMdoi
10.1002/jemt.22545subject
Has Abstractpub_date
2015-09-01 00:00:00pages
823-30issue
9eissn
1059-910Xissn
1097-0029journal_volume
78pub_type
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