Abstract:
:Protein expression patterns are a primary determinant of tissue function and in this study we developed methods to study protein expression over macroscopic distances at subcellular levels of detail. Using the mammalian lens as a model tissue system, we show that by combining two-photon microscopy with novel image montage methods (fast beam blanking coupled with mathematical alignment tools) we have extended the limited field of view of laser scanning microscopes. To illustrate the utility of our approach, the distribution of connexin-46 was visualized across equatorial sections of the rat mammalian lens. By optimizing fixation protocols, good morphological preservation could be achieved over the thickness of the lens (approximately 4 mm) while preserving antigenicity of lens proteins. Using the same image data, changes in lens fiber cell morphology were mapped quantitatively by automatic image analysis routines. The methods presented should be generally applicable to any tissue system where changes in antibody labeling and tissue structure occur over large and small distances.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Jacobs MD,Donaldson PJ,Cannell MB,Soeller Cdoi
10.1002/jemt.10360keywords:
subject
Has Abstractpub_date
2003-09-01 00:00:00pages
83-91issue
1eissn
1059-910Xissn
1097-0029journal_volume
62pub_type
杂志文章abstract::Morphological studies of the microcirculatory system in the thymus were reviewed in regards to methodology and structural organization of blood and lymphatic vessels. The blood capillaries and postcapillary venules (PCVs) in the thymus are characterized by a double-walled structure. These vessels are surrounded more o...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19970801)38:3<287::AID-JEM
更新日期:1997-08-01 00:00:00
abstract::This research aimed to obtain the depth dependence of polymerization contraction and microporosity from irradiated dental resin cements by X-ray computed microtomography (μCT). Samples (n = 5) of commercial Relyx U200 (RU) and AllCem Core (AC) dual-cure resin cements were injected in a cylindrical Teflon sampler (25 m...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23456
更新日期:2020-06-01 00:00:00
abstract::This paper presents a snapshot view of the influence and direction of microcomputer technology for image analysis techniques in diagnostic pathology. Microcomputers have had considerable impact in bringing image analysis to wider application. Semi-automated tracing techniques are a simple means of providing objective ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070210406
更新日期:1992-06-01 00:00:00
abstract::In present study, multiple microscope techniques were used for the systematics identification of the species Asplenium dalhousiae. The plant was collected from different phytogeographical and its natural habitat of Pakistan, where it shows higher diversity. Morphology, foliar epidermal anatomy, and spore morphological...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23189
更新日期:2019-04-01 00:00:00
abstract::A variety of intracellular recording and staining techniques has been used to establish structure-function and, in some cases, structure-function-neurochemical correlations in fish, turtle, and cat retinae. Cone photoreceptor-horizontal cell connectivity has been studied extensively in the cyprinid fish retina by intr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070240106
更新日期:1993-01-01 00:00:00
abstract::Using the optical methods described, phase specimens can be observed with a modified light microscope in enhanced clarity, purified from typical artifacts which are apparent in standard phase contrast illumination. In particular, haloing and shade-off are absent, lateral and vertical resolution are maximized and the i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22265
更新日期:2013-10-01 00:00:00
abstract::An analytical result is given for the wavelength at which two or more first order Laue zone (FOLZ) lines intersect at a single point on the positive x axis when a face centred cubic crystal is observed in the convergent beam mode with the beam axis parallel to the [100] direction. The position of the intersection alon...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070290311
更新日期:1994-10-15 00:00:00
abstract::The autonomic neuromuscular junction at a varicosity in the vas deferens is defined by the localization of the vesicle-associated protein syntaxin in high concentrations in the axolemma and a high density of P2x1 receptors in a cluster beneath the varicosity. Calcium fluxes have been observed in all individual varicos...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19980915)42:6<433::AID-JEM
更新日期:1998-09-15 00:00:00
abstract::The subcellular behavior of aluminum and indium, used in medical and industrial fields, was studied in the gastric mucosa and the liver after their intragastric administration to rats, using, two of the most sensitive methods of observation and microanalysis, the transmission electron microscopy, and the secondary ion...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.21041
更新日期:2012-02-01 00:00:00
abstract::This study compared several irrigation protocols and application systems for sealer penetration into dentinal tubules. Single-rooted-human teeth were divided into 5 experimental groups (n = 15) and a control group (n = 5), according to final irrigation protocols: standard needle irrigation (SNI); Vibringe; Vibringe + ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22944
更新日期:2017-12-01 00:00:00
abstract::The present study was conducted on characterization of morpho-anatomical, phytochemical, and bio-elemental analysis of root, stem, and leaf of Verbascum thapsus. Morphologically Verbascum is a biennial plant that flowers for a month and a half in mid- to late summer. Various organoleptic features of root, leaf, and st...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23454
更新日期:2020-06-01 00:00:00
abstract::Cetoniinae is one of the showiest scarab groups, exhibiting bright-metallic body colors, and usually attract great attention from entomologists and amateur collectors. Larvae of Cetoniinae show dramatically diversity on morphology and living habits. Although being considered one of the best-studied groups of Scarabaei...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23594
更新日期:2021-02-01 00:00:00
abstract::This experiment was conducted in Ile-de-France adult rams to examine the target point of a 2-month light cycle regimen on seminiferous tubule functions, on intertubular compartment and on Leydig cell parameters. Eight rams were subjected to a 2-month light cycle regimen and were compared to sexually active or inactive...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070200306
更新日期:1992-02-01 00:00:00
abstract::Numerous studies using morphologic techniques have demonstrated the plasticity of pulmonary epithelial cells and the requirement for complex intercellular interactions for regeneration of normal epithelium following lung injury. Recent developments in the molecular characterization of genes expressed in the lung have ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970901)38:5<473::AID-JEM
更新日期:1997-09-01 00:00:00
abstract::Central to the control of vascular resistance in the systemic and pulmonary circulation and at the solid organ level is the function of the endothelial-derived vasomediators, endothelin-1 (ET-1) and nitric oxide. Regulation of steady-state levels of ET-1 and endothelial nitric oxide synthase (eNOS) mRNAs represents an...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10242
更新日期:2003-01-01 00:00:00
abstract::Infection of humans with Helicobacter pylori results in the development of chronic gastritis and plays an important role in gastric ulcer pathogenesis. Despite the infiltration of the mucosa with specific immunocompetent cells and production of specific antibodies, the infection usually persists for life. This study w...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1111
更新日期:2001-06-15 00:00:00
abstract::Ion transport mediated by osmotically induced chloride channels in HT29 cells was investigated by X-ray microanalysis of cell cultures. The cells were cultured on cellulose nitrate filters, and exposed to isotonic, hypotonic, or hypertonic solutions. Then the NaCl-rich medium was removed by rinsing the cells with a wa...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980101)40:1<72::AID-JEMT
更新日期:1998-01-01 00:00:00
abstract::Natriuretic peptides (NP), together with nitric oxide (NO) are powerful relaxing factors acting via a common second messenger, cyclic GMP (cGMP). Together with other vasoactive modulators, these vasorelaxing factors play an essential role in regulating the function of kidney glomeruli. The presence of NP receptors in ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10080
更新日期:2002-05-15 00:00:00
abstract::In spite of accumulating evidence for a modulation of sympathetic neurotransmission by endogenously produced nitric oxide (NO), it remains unclear in which parts of the vascular system and at what level this interaction takes place. The aim of the present study was to investigate the distribution of endothelial and ne...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070290214
更新日期:1994-10-01 00:00:00
abstract::Growth-year authentication has extraordinary significance for plant growth, structure and development research, and has a wide range of applications in value assessment of economic crops. Panax ginseng is the most commonly used medicinal plant in Asian countries. The fix number of growth-year is an important quality e...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22610
更新日期:2016-02-01 00:00:00
abstract::The catecholaminergic cell groups in the human brain, denominated from A1 to A17, display some striking anatomical differences with those described in the rodent. These differences are essentially observed in the extent of the dopaminergic neurons and especially their axonal fields in the telencephalon. Immunocytochem...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990701)46:1<24::AID-JEMT
更新日期:1999-07-01 00:00:00
abstract::Brain tumor identification using magnetic resonance images (MRI) is an important research domain in the field of medical imaging. Use of computerized techniques helps the doctors for the diagnosis and treatment against brain cancer. In this article, an automated system is developed for tumor extraction and classificat...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23238
更新日期:2019-06-01 00:00:00
abstract::Worldwide, breast cancer (BrCa) is currently the leading cause of deaths associated to malignant lesions in adult women. Given that some studies have mentioned that peritumoral adipocytes may contribute to breast carcinogenesis, present work sought to quantitative evaluate the morphometry of these cells in a group of ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22972
更新日期:2018-02-01 00:00:00
abstract::This study investigated the relationship among heat shock protein 70 (HSP70), proliferating cell nuclear antigen (PCNA), and testicular apoptosis during a breeding cycle of Prochilodus argenteus, a neotropical migratory characiform fish of importance in commercial fishery from the São Francisco River basin. A total of...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22173
更新日期:2013-04-01 00:00:00
abstract::The possibility that tannic acid and other classical mordants can be used in the electron microscopical section staining technique has been tested. A mordant can be defined as a chemical that combines with both a certain specific tissue component and the staining substance and thereby permits a staining reaction that ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070210110
更新日期:1992-03-01 00:00:00
abstract::Isoform diversity in striated muscle is largely controlled at the level of transcription. In this review we will concentrate on studies concerning transcriptional regulation of the alkali myosin light chain 1F/3F gene. Uncoupled activity of the MLC1F and 3F promoters, together with complex patterns of transcription in...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20000915)50:6<510::AID-JEMT8>3.0
更新日期:2000-09-15 00:00:00
abstract::This was the first comprehensive pollen micromorphological investigation of lactiferous flora (Apocynaceae) of District Rawalpindi, Pakistan. The pollen morphology of 10 species of the family Apocynaceae was observed and documented using light microscopy and scanning electron microscopy. Pollen was found subspheroidal...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23293
更新日期:2019-09-01 00:00:00
abstract::Atomic force microscopy has been used to study self-assembled structures of two alpha-hemolysin mutants. For a mutant (alphaHL-H5) that was locked into the prepore state on fluid phase egg-PC membranes, we visualized, for the first time, heptameric prepores and showed that the 7-fold axis in the prepore lies perpendic...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990301)44:5<353::AID-JEM
更新日期:1999-03-01 00:00:00
abstract::This paper summarizes the essential features of the various facilities dedicated to in situ irradiation effects research around the world at the present time, mentions some essential techniques that are involved in this type of research, and describes very briefly some examples of studies employing these facilities an...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19980915)42:4<255::AID-JEM
更新日期:1998-08-15 00:00:00
abstract::Filamentous bacteriophage has been proposed as a vehicle that can carry and deliver therapeutics into mammalian cells for disease treatment, thus a protocol for imaging phage-cell interaction is essential. Because high signal intensity is necessary to study the mechanism of interaction between filamentous bacteriophag...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20793
更新日期:2010-05-01 00:00:00