Sympathetic neuromuscular transmission at a varicosity in a syncytium.

Abstract:

:The autonomic neuromuscular junction at a varicosity in the vas deferens is defined by the localization of the vesicle-associated protein syntaxin in high concentrations in the axolemma and a high density of P2x1 receptors in a cluster beneath the varicosity. Calcium fluxes have been observed in all individual varicosities of a nerve terminal on the arrival of an impulse even though recordings made from these varicosities of the electrical signs of transmission with loose-patch electrodes over the varicosities show that they have very different probabilities for the secretion of a quantum. The fact that some varicosities seldom release a quantum on the arrival of an impulse is supported by the observation that antibodies against the N-terminus of synaptotagmin, which uniquely label the inside of synaptic vesicles when they undergo exocytosis, fail to do so in some varicosities during nerve stimulation whereas they do in others. It is suggested that the probability for secretion from a varicosity depends on the number of secretosomes that the varicosity possesses, where a secretosome is a complex of syntaxin, synaptotagmin, an N-type calcium channel, and a synaptic vesicle.

journal_name

Microsc Res Tech

authors

Bennett MR,Cheung A,Brain KL

doi

10.1002/(SICI)1097-0029(19980915)42:6<433::AID-JEM

subject

Has Abstract

pub_date

1998-09-15 00:00:00

pages

433-50

issue

6

eissn

1059-910X

issn

1097-0029

pii

10.1002/(SICI)1097-0029(19980915)42:6<433::AID-JEM

journal_volume

42

pub_type

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