Diffracted-light contrast enhancement: a re-examination of oblique illumination.

Abstract:

:A re-examination and modification of the very old oblique illumination technique has resulted in a method for contrast enhancement in microscopes, diffracted-light contrast (DLC), which provides high-contrast, high-resolution images of unstained biological material. The technique, which utilizes the diffracted light from the edge of a small, opaque plate, provides shadowcast images similar to those obtained by Nomarski DIC, anaxial illumination, modulation contrast, or single-sideband microscopy; however, it requires only a single additional component, which can be added to any bright field microscope. The contrast and three-dimensionality of the final image can be controlled by inserting differently shaped edges. Any bright field condenser will work with the technique and, consequently, it is a technique that may be especially useful with relatively basic, inexpensive laboratory and teaching microscopes although the image produced on a research grade microscope is of very high quality, comparable to that obtained with DIC.

journal_name

Microsc Res Tech

authors

Piekos WB

doi

10.1002/(SICI)1097-0029(19990815/01)46:4/5<334::AI

keywords:

subject

Has Abstract

pub_date

1999-08-15 00:00:00

pages

334-7

issue

4-5

eissn

1059-910X

issn

1097-0029

pii

10.1002/(SICI)1097-0029(19990815/01)46:4/5<334::AI

journal_volume

46

pub_type

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