Abstract:
:A re-examination and modification of the very old oblique illumination technique has resulted in a method for contrast enhancement in microscopes, diffracted-light contrast (DLC), which provides high-contrast, high-resolution images of unstained biological material. The technique, which utilizes the diffracted light from the edge of a small, opaque plate, provides shadowcast images similar to those obtained by Nomarski DIC, anaxial illumination, modulation contrast, or single-sideband microscopy; however, it requires only a single additional component, which can be added to any bright field microscope. The contrast and three-dimensionality of the final image can be controlled by inserting differently shaped edges. Any bright field condenser will work with the technique and, consequently, it is a technique that may be especially useful with relatively basic, inexpensive laboratory and teaching microscopes although the image produced on a research grade microscope is of very high quality, comparable to that obtained with DIC.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Piekos WBdoi
10.1002/(SICI)1097-0029(19990815/01)46:4/5<334::AIkeywords:
subject
Has Abstractpub_date
1999-08-15 00:00:00pages
334-7issue
4-5eissn
1059-910Xissn
1097-0029pii
10.1002/(SICI)1097-0029(19990815/01)46:4/5<334::AIjournal_volume
46pub_type
杂志文章abstract::We compared Hall's peak to continuum ratio method with a peak ratio method in order to quantify light elements (C, N, and O) in organic specimens as a model for biological thin sections. X-ray spectra were recorded by an energy dispersive X-ray spectrometer equipped with an ultra thin window detector. Spectra were pro...
journal_title:Microscopy research and technique
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abstract::Uranium-contaminated soils from the U.S. Department of Energy (DOE) Fernald Site, Ohio, have been examined by a combination of backscattered electron imaging (BSE) and analytical electron microscopy with electron diffraction (AEM). The inhomogeneous distribution of particulate uranium phases in the soil required the d...
journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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更新日期:2006-05-01 00:00:00
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更新日期:1998-07-01 00:00:00
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journal_title:Microscopy research and technique
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doi:10.1002/jemt.23470
更新日期:2020-07-01 00:00:00
abstract::Virus particles and viral inclusions were detected by transmission electron microscopy examination of sections of the seminal vesicles and mucus gland of asymptomatic young drones from colonies of Apis mellifera lightly infested by Varroa mite. In the mucus gland the infection was found in the muscular sheath and epit...
journal_title:Microscopy research and technique
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doi:10.1002/jemt.22024
更新日期:2012-07-01 00:00:00
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journal_title:Microscopy research and technique
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更新日期:1998-12-01 00:00:00
abstract::The fine structure of human oogonia and growing oocytes has been reviewed in fetal and adult ovaries. Preovulatory maturation and the ultrastructure of stimulated oocytes from the germinal vesicle (GV) stage to metaphase II (MII) stage are also documented. Oogonia have large nuclei, scanty cytoplasm with complex mitoc...
journal_title:Microscopy research and technique
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doi:10.1002/jemt.20299
更新日期:2006-06-01 00:00:00
abstract::The region-specific angioarchitecture of the bovine pododerma was examined using systematic scanning electron microscopy of micro-corrosion casts of juvenile and adult bovine claws. Particular emphasis was laid on the demonstration of specialised vascular structures such as arteriovenous anastomoses. Comparing the res...
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更新日期:2001-09-15 00:00:00
abstract::The intravacuolar network inside the parasitophorous vacuole of Toxoplasma gondii consists of an intricate system of membrane-limited tubules of uncertain role in parasite development. We propose that it is an important structural support to the maintenance of the parasites in the characteristic rosette arrangement of...
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更新日期:1996-08-01 00:00:00
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更新日期:2009-07-01 00:00:00
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journal_title:Microscopy research and technique
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abstract::Chronic kidney disease (CKD) is a relevant disease in feline clinic. The tubulointerstitial damage, with collagen deposition and fibrosis, is an important result of this process. The aim of this study was to quantify and correlate the deposition of collagen and severity of interstitial fibrosis (IF) in the kidney from...
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journal_title:Microscopy research and technique
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更新日期:2000-05-01 00:00:00
abstract::The aim of this study is to evaluate the antitumor effect of indirubin-3-monoxime and its mode of action in benzo(α)pyrene [B(α)P] induced lung cancer in A/J mice. Light microscopic examination of lung sections of [B(α)P] induced lung cancer mice revealed the presence of adenocarcinoma characterized by extensive proli...
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journal_title:Microscopy research and technique
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abstract::The effect of nerve growth factor (NGF) on ontogenesis of frog mast cells was investigated in vivo by histochemical, morphometric, and ultrastructural analysis. Three groups of tadpoles at various stages of development were used. In the first group, the larvae received i.p. injections of 1 ng NGF/g; the second group r...
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