Abstract:
:The through focus exit wave reconstruction technique uses a series of high resolution electron microscopy images to reconstruct the complex electron wavefunction at the exit plane of the specimen. The feasibility of the through focus exit wave reconstruction method and its most important limitations are discussed. It is shown that-provided all aberrations of the microscope are well corrected for-a large improvement in the interpretability of the information can be obtained.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Zandbergen HW,Van Dyck Ddoi
10.1002/(SICI)1097-0029(20000501)49:3<301::AID-JEMkeywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
301-23issue
3eissn
1059-910Xissn
1097-0029pii
10.1002/(SICI)1097-0029(20000501)49:3<301::AID-JEMjournal_volume
49pub_type
杂志文章abstract::Automated detection and classification of gastric infections (i.e., ulcer, polyp, esophagitis, and bleeding) through wireless capsule endoscopy (WCE) is still a key challenge. Doctors can identify these endoscopic diseases by using the computer-aided diagnostic (CAD) systems. In this article, a new fully automated sys...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23447
更新日期:2020-05-01 00:00:00
abstract::In the way of making graphene an industry-friendly material, it must be mass-produced with high-quality and reduced cost over large areas. Assisted by machine-learning techniques, rapid, nondestructive and accurate determination of large graphene sheets on SiO2 /Si substrates has been made possible in recent years by ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23505
更新日期:2020-09-01 00:00:00
abstract::Historically, there has been much debate on the nature of infantile hemangiomas as either congenital malformations or benign neoplasms. Some vascular lesions that are present at birth and evidence no proliferative growth are considered to be congenital malformations; other post-natal vascular tumors pursue aggressive ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10254
更新日期:2003-02-01 00:00:00
abstract::The apolipoprotein E (apoE) epsilon4 allele increases risk of Alzheimer's disease (AD), perhaps by accelerating plaque formation, or by impairing neuron repair. Considerable evidence supports both mechanisms. AD patients with epsilon4 have more and earlier amyloid deposits than do patients without epsilon4. The same i...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20000815)50:4<278::AID-JEMT5>3.0
更新日期:2000-08-15 00:00:00
abstract::The present study focused to elucidate the promising role of probiotics in the fish culture, by employing microscopic and biochemical parameters to rationalize the possible outcomes. After preliminary feeding trial histopathological analysis revealed clear and visible changes in the intestinal and liver cells of CTRL ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23416
更新日期:2020-03-01 00:00:00
abstract::Basement membranes (BMs) were first described in the mid-19th century, but they were not isolated and prepared for compositional studies until nearly 100 years later. Early methods of isolation were carried out on renal glomeruli, which were first sub-fractionated from kidney tissues by sieving. BMs were then isolated...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070280302
更新日期:1994-06-15 00:00:00
abstract::Ultramicrotomy has found novel application in materials research using transmission electron microscopy, for both analytical and high resolution work. Specimens 20-50 nm in thickness with lateral dimensions of up to 100 microns may routinely be prepared, with the additional advantage of precise area location for cross...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070310404
更新日期:1995-07-01 00:00:00
abstract::We have examined cellular events at the early stages of periosteal chondrogenesis and osteogenesis induced by bone fracture, using a well-standardized rib fracture model of the mouse. The initial cellular event was recognized as considerable proliferation in the deeper layer referred to as the "cambium layer" of the p...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20088
更新日期:2004-07-01 00:00:00
abstract::The photoreceptor population in Xenopus consists of a green-sensitive rod (lambda(max) = 523 nm), a blue-sensitive rod (lambda(max) = 445 nm) and three classes of cone. The largest cone is red-sensitive (lambda(max) = 611 nm). The intermediate cone is presumed to be blue-sensitive based on physiological criteria, wher...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20000901)50:5<338::AID-JEMT3>3.0
更新日期:2000-09-01 00:00:00
abstract::We have used high voltage electron microscopy and computer-generated three-dimensional reconstructions from serial sections to elucidate the structure of taste bud cells and their associated synapses in fungiform taste buds of the mouse. Five fungiform taste buds (two of which were serially sectioned) were examined wi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070260207
更新日期:1993-10-01 00:00:00
abstract::All termite species (Isoptera) are eusocial. These insects live in societies where the division of labor is a predominant characteristic, conditioned by the presence of castes with different morphology, ontogeny, and development. The soldier caste is unique among social insects and it is responsible for colony defense...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20365
更新日期:2006-11-01 00:00:00
abstract::Frozen skin sections are routinely used for light microscopic immunohistochemical study of the skin basement membrane zone for two reasons: some skin basement membrane zone proteins are labile to routine chemical fixation, and skin is not amenable to vibratome sectioning. However, inherent limitations of conventional ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1136
更新日期:2001-08-15 00:00:00
abstract::3D surface profiling and high resolution imaging were performed to refine the Florin rings and epicuticular wax crystals of Pinus koraiensis needles. Needles were collected from four-year-old seedlings and air-dried for surface observations. Field emission scanning electron microscopy revealed that stomata were found ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.21010
更新日期:2011-12-01 00:00:00
abstract::Understanding the functional morphology and mobility of appendages of fossil animals is important for exploring ecological traits such as feeding and locomotion. Previous work on fossils from the 518 million-year-old Chengjiang biota of China was based mainly on two-dimensional information captured from the surface of...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23628
更新日期:2020-11-06 00:00:00
abstract::Among the five senses, the sense of smell (olfaction) is the most sensitive and emotional window on the outside world (Stern and Marx, 1999). The olfactory system recognizes and discriminates myriad odorants of diverse molecular structures. What makes the olfactory system so specific and sensitive? OE harboring the ol...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10130
更新日期:2002-08-01 00:00:00
abstract::The fine structure of human oogonia and growing oocytes has been reviewed in fetal and adult ovaries. Preovulatory maturation and the ultrastructure of stimulated oocytes from the germinal vesicle (GV) stage to metaphase II (MII) stage are also documented. Oogonia have large nuclei, scanty cytoplasm with complex mitoc...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20299
更新日期:2006-06-01 00:00:00
abstract::Cell-free extracts of Xenopus eggs were used to investigate how differing lamin sub-types are incorporated into a lamina and how lamina composition influences DNA replication. Purified recombinant human lamin A (HlaminA) was inoculated into egg extracts, which support in vitro nuclear assembly. The route of incorporat...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990401)45:1<1::AID-JEMT1
更新日期:1999-04-01 00:00:00
abstract::Acquisition of a great number of energy-filtered images in a TEM (EFTEM) around the characteristic signal with a low energy-selecting slit allows display of the electron energy loss (EEL)-spectrum of regions of interest (ROIs) of a sample. These EEL-spectra can be submitted to the different treatments already in use f...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1079
更新日期:2001-04-15 00:00:00
abstract::Red myofibers in mouse soleus muscle have two spatially distinct populations of mitochondria: one where these organelles are disposed in large clusters just inside the sarcolemma and the other situated between the myofibrils. In most cases, the interfibrillar mitochondria (IFM), which are much smaller than the subsarc...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22158
更新日期:2013-03-01 00:00:00
abstract::To compare the smear layer removal ability and mineral content of root canal dentine after initial irrigation with NaOCl and final irrigation with MTAD, QMix, and 17% EDTA. Forty extracted human maxillary incisors before root canal preparation and irrigation with NaOCl were randomly divided into four groups (n = 10) a...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23239
更新日期:2019-06-01 00:00:00
abstract::Fipronil, active ingredient of Frontline® acaricide, is a phenyl-pyrazolic derivative, and its efficacy in the elimination of several plagues, even in lower concentrations, has already been demonstrated; however, its effect on nontarget organisms has not been thoroughly explained. In this sense, the present study had ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.21053
更新日期:2012-03-01 00:00:00
abstract::Tapping mode atomic force microscopy (AFM) provides phase images in addition to height and amplitude images. Although the behavior of tapping mode AFM has been investigated using mathematical modeling, comprehensive understanding of the behavior of tapping mode AFM still poses a significant challenge to the AFM commun...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22558
更新日期:2015-10-01 00:00:00
abstract::Detailed maps of the A-T distribution within human mitotic chromosomes labeled with BrdU are obtained with a high resolution scanning ion microprobe through the detection of bromine by imaging secondary ion mass spectrometry (SIMS). Corresponding maps of the emission loci of the molecular ion CN- describe the overall ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19970215)36:4<301::AID-JEM
更新日期:1997-02-15 00:00:00
abstract::This article presents an integrated approach for the authentication of five Russian icons through the study of their paint materials (elemental composition and stratigraphic mapping), state of conservation and artistic technique. The results of the applied analytical protocol, involving optical and scanning electron m...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20727
更新日期:2009-10-01 00:00:00
abstract::Tungsten single atoms and clusters composed of four and five atoms deposited on MgO (001) thin films were observed by high-resolution transmission electron microscopy at R.T. The observation was realized by optimizing the thickness of the MgO films and by using special imaging techniques such as an off-Bragg HREM meth...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980215)40:4<265::AID-JEM
更新日期:1998-02-15 00:00:00
abstract::The dynamic transmission electron microscope (DTEM) is introduced as a novel tool for in situ processing of materials. Examples of various types of dynamic studies outline the advantages and differences of laser-based heating in the DTEM in comparison to conventional (resistive) heating in situ TEM methods. We demonst...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20664
更新日期:2009-03-01 00:00:00
abstract::The intravacuolar network inside the parasitophorous vacuole of Toxoplasma gondii consists of an intricate system of membrane-limited tubules of uncertain role in parasite development. We propose that it is an important structural support to the maintenance of the parasites in the characteristic rosette arrangement of...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20182
更新日期:2005-05-01 00:00:00
abstract::Most of the molecular and experimental studies on the floor plate (FP) have been performed on the FP region extending along the spinal cord. However, little is known about the hindbrain FP. The FP undergoes regional and temporal changes throughout development, but information with respect to the ultrastructural correl...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/1097-0029(20010301)52:5<615::AID-JEMT1045>
更新日期:2001-03-01 00:00:00
abstract::We observed the corrosion casts of the Wistar rats' pancreatic ducts with scanning electron microscopy (SEM), and their conventionally fixed pancreatic tissue with SEM and transmission electron microscopy (TEM). These findings revealed the following facts about the three-dimensional structure of pancreatic duct. (1) T...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19970601)37:5/6<543::AID-J
更新日期:1997-06-01 00:00:00
abstract::During late anaphase and telophase, animal cells develop a bundle of antiparallel, interdigitating microtubules between the two daughter nuclei. Recent data indicate that this structure, called the central spindle, plays an essential role during cytokinesis. Studies in Drosophila and on vertebrate cells strongly sugge...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(20000415)49:2<202::AID-JEM
更新日期:2000-04-15 00:00:00