Laser-based in situ techniques: novel methods for generating extreme conditions in TEM samples.

abstract：:Chronic kidney disease (CKD) is a relevant disease in feline clinic. The tubulointerstitial damage, with collagen deposition and fibrosis, is an important result of this process. The aim of this study was to quantify and correlate the deposition of collagen and severity of interstitial fibrosis (IF) in the kidney from...

journal_title：Microscopy research and technique

authors： Morais GB,Viana DA,Silva FMO,Xavier Júnior FAF,Farias KM,Pessoa CD,Silveira JAM,Alves APNN,Mota MRL,Silva FDO,Sampaio CMS,Verdugo JMG,Evangelista JSAM

更新日期：2017-05-01 00:00:00

abstract：:The lateral mobility of membrane receptors provides insights into the molecular interactions of protein binding and the complex dynamic plasma membrane. The image mean square displacement (iMSD) analysis is a method used to extract qualitative and quantitative information of the protein diffusion law and infers how di...

journal_title：Microscopy research and technique

authors： Planes N,Vanderheyden PPML,Gratton E,Caballero-George C

更新日期：2020-04-01 00:00:00

abstract：:The principal functions of the gastrointestinal tract mucosa include nutrient absorption, protein and fluid secretion, and the regulated symbiosis with intraluminal contents. Research in epithelial biology has benefited significantly from the use of cultured monolayer preparations, which closely replicate the structur...

journal_title：Microscopy research and technique

authors： Gilbert RJ,Hoffman M,Capitano A,So PT

更新日期：2000-10-15 00:00:00

abstract：:The cyclic fluctuations in circulating levels of 17 beta-estradiol and progesterone that occur during the menstrual or estrous cycle are responsible for dramatic, cyclic changes in the epithelial lining and secretory status of the mammalian oviduct. The timely transition in the synthesis and release of oviduct protein...

journal_title：Microscopy research and technique

authors： Murray MK,DeSouza MM,Messinger SM

更新日期：1995-08-15 00:00:00

abstract：:The morphology and ultrastructure of the rectum in Poecilimon cervus Karabağ, 1950 (Orthoptera, Tettigoniidae) were analyzed by light microscope, scanning (SEM) and transmission electron microscopes (TEM). The rectum is the final part of the digestive tract that plays an important role in water reabsorption in insects...

journal_title：Microscopy research and technique

authors： Polat I,Suludere Z,Candan S

更新日期：2017-02-01 00:00:00

abstract：:Effective protocols for controlling crystal structure, size, and morphology attract considerable interest given the requirement for particles of modal size and shape in many areas of materials fabrication and the importance of crystallochemical selectivity in determining the exploitable properties of inorganic solids....

journal_title：Microscopy research and technique

authors： Heywood BR

更新日期：1994-04-01 00:00:00

abstract：:Nonlinear microscopy techniques are being increasingly used to perform in vivo studies in dermatology. These methods enable us to investigate the morphology and monitor the physiological process in the skin by the use of femtosecond lasers operating in the red, near-infrared spectral range (680-1,300 nm). In this work...

journal_title：Microscopy research and technique

authors： Haluszka D,Lőrincz K,Molnár G,Tamás G,Kolonics A,Szipőcs R,Kárpáti S,Wikonkál NM

更新日期：2015-09-01 00:00:00

abstract：:Most of the molecular and experimental studies on the floor plate (FP) have been performed on the FP region extending along the spinal cord. However, little is known about the hindbrain FP. The FP undergoes regional and temporal changes throughout development, but information with respect to the ultrastructural correl...

journal_title：Microscopy research and technique

authors： del Brio MA,Riera P,Peruzzo B,Rodríguez EM

更新日期：2001-03-01 00:00:00

abstract：:Spermatogenesis is a process of division and differentiation by which spermatozoa are produced in seminiferous tubules. A measure of efficiency of spermatogenesis is the estimated number of spermatozoa produced per day per gram of testicular parenchyma. This measure is not influenced by species differences in testicul...

journal_title：Microscopy research and technique

authors： Johnson L

更新日期：1995-12-01 00:00:00

abstract：:In vivo and in vitro multiphoton imaging was used to perform high resolution optical sectioning of human hair by nonlinear excitation of endogenous as well as exogenous fluorophores. Multiphoton fluorescence lifetime imaging (FLIM) based on time-resolved single photon counting and near-infrared femtosecond laser pulse...

journal_title：Microscopy research and technique

authors： Ehlers A,Riemann I,Stark M,König K

更新日期：2007-02-01 00:00:00

abstract：:The mammalian zona pellucida (ZP) is an extracellular matrix surrounding oocytes and early embryos, which is critical for normal fertilization and preimplantation development. It is made up of three/four glycoproteins arranged in a delicate filamentous matrix. Scanning electron microscopy (SEM) studies have shown that...

journal_title：Microscopy research and technique

authors： Familiari G,Relucenti M,Heyn R,Micara G,Correr S

更新日期：2006-06-01 00:00:00

abstract：:A light and electron microscopic study was performed to determine age changes in Meissner corpuscles. In forepaw digital pads of mice aged to their maximum life expectancy, corpuscles were found to increase in size and complexity until middle age, and then to become smaller, disorganized and lobulated with more advanc...

journal_title：Microscopy research and technique

authors： Nava PB,Mathewson RC

更新日期：1996-07-01 00:00:00

abstract：:Currently, exploration of alternative energy resources is hotly debated among the scientific community owing to rising energy crises and environmental issues. Biodiesel, as renewable energy source proves to be a better option and substitute to petro diesel. In this regard, nonedible seeds could be a better feedstock f...

journal_title：Microscopy research and technique

authors： Munir M,Ahmad M,Waseem A,Zafar M,Saeed M,Wakeel A,Nazish M,Sultana S

更新日期：2019-07-01 00:00:00

abstract：:The present study provides experimental data which indicate that the neutrophil is ideal for studying programmed cell death or apoptosis in vitro. Neutrophils can be obtained from human peripheral blood in large numbers with minimal experimental manipulation and are easily separated from other leukocytes, providing ne...

journal_title：Microscopy research and technique

authors： Payne CM,Glasser L,Tischler ME,Wyckoff D,Cromey D,Fiederlein R,Bohnert O

更新日期：1994-07-01 00:00:00

abstract：:Although the Geographical Information System (GIS), which integrates computerized drawing computer assisted design (CAD) and relational databases (data base management system (DBMS)), is best known for applications in geographical and planning cartography, it can also use many kinds of information concerning the terri...

journal_title：Microscopy research and technique

authors： Scocco P,Brusaferro A,Catorci A

更新日期：2012-07-01 00:00:00

abstract：:In certain cases of infertility in domestic species, and in the homozygous c-ros tyrosine kinase knockout mouse, males are infertile as a result of unopposed sperm swelling. This induces flagellar angulation, preventing their normal migration in the female tract. Angulation always occurs at the site of the cytoplasmic...

journal_title：Microscopy research and technique

authors： Cooper TG,Yeung CH

更新日期：2003-05-01 00:00:00

abstract：:We present a semiautomated software solution to the problem of extending the lateral field of view of a classical microscope. The initial requirements are a set of overlapping images, along with their user-provided coarse mosaic. Our solution then refines this initial mosaic in a fully automatic fashion. We rely on a ...

journal_title：Microscopy research and technique

authors： Thévenaz P,Unser M

更新日期：2007-02-01 00:00:00

abstract：:Twelve substrains of inbred senescence-accelerated mice (SAM) have been developed, among which the SAMP8 and SAMP10 strains show a significant age-related deterioration in learning and memory for passive and active avoidance tasks. These strains have, respectively, a low and high incidence of systemic senile amyloidos...

journal_title：Microscopy research and technique

authors： Kawamata T,Akiguchi I,Maeda K,Tanaka C,Higuchi K,Hosokawa M,Takeda T

更新日期：1998-10-01 00:00:00

abstract：:This paper summarizes the essential features of the various facilities dedicated to in situ irradiation effects research around the world at the present time, mentions some essential techniques that are involved in this type of research, and describes very briefly some examples of studies employing these facilities an...

journal_title：Microscopy research and technique

authors： Allen CW,Ryan EA

更新日期：1998-08-15 00:00:00

abstract：:Classical conditioning (CC) with a sweet flavored is useful for experimental models of immune response. The objective this work was analyze spleen cell response on the differentiation of B-lymphocytes associated with the CC with sweet flavored in adult rats. Twelve adult male rats were divided (n = 3) in Group 1 (cont...

journal_title：Microscopy research and technique

authors： Rodríguez Bustos H,Espinoza-Navarro O,Castro ME

更新日期：2018-12-01 00:00:00

abstract：:An understanding of the internal morphology of the ant mandible is important in explaining the relationship between the exocrine system and the behavioral and phylogenetic characteristics of different subfamilies of Formicidae. In this study, we investigated the occurrence of intramandibular glands in ants from the Po...

journal_title：Microscopy research and technique

authors： Martins LC,Serrão JE

更新日期：2011-08-01 00:00:00

abstract：:The opossum Monodelphis domestica presents movement of the temporomandibular joint (TMJ) reflecting adaptation to eating habits similar to movement in humans, but the structure of the TMJ is not yet known. Thus, nine young M. domestica, of both sexes were weighed, anesthetized with xylazine (10 mg kg(-1) ), and ketami...

journal_title：Microscopy research and technique

authors： Minucci MS,Issa JP,Yokoyama FY,Dias FJ,Iyomasa DM,Guimarães EA,Watanabe IS,Iyomasa MM

更新日期：2016-09-01 00:00:00

abstract：:Gymnosporia arenicola Jordaan (Celastraceae) is a shrub or small tree, which naturally occurs in coastal sand dunes of Southern Mozambique and South Africa. Its dried leaf is often used in traditional medicine for the treatment of infectious and inflammatory diseases. Hereby, we present results of studies carried out ...

journal_title：Microscopy research and technique

authors： Da Silva G,Serrano R,Gomes ET,Silva O

更新日期：2015-11-01 00:00:00

abstract：:This ultrastructural study showed that nonisotopic methanolic hafnium chloride and aqueous lead solution was an excellent new electron stain for enhancing TEM contrasts of fungal and plant cell structures. The ultrastructural definition provided by the new stain was often superior to that provided by conventional stai...

journal_title：Microscopy research and technique

authors： Ikeda K,Inoue K,Kanematsu S,Horiuchi Y,Park P

更新日期：2011-09-01 00:00:00

abstract：:After more than two centuries of almost sporadic inquiry as to the existence and function of the human vomeronasal system (VNS), the last decade has seen a resurgent interest in it. The principal question vexing many laboratories is whether adult humans retain the VNS that clearly develops during fetal growth. Additio...

journal_title：Microscopy research and technique

authors： Johnson EW

更新日期：1998-06-15 00:00:00

abstract：:Understanding the functional morphology and mobility of appendages of fossil animals is important for exploring ecological traits such as feeding and locomotion. Previous work on fossils from the 518 million-year-old Chengjiang biota of China was based mainly on two-dimensional information captured from the surface of...

journal_title：Microscopy research and technique

authors： Schmidt M,Liu Y,Zhai D,Hou X,Melzer RR

更新日期：2020-11-06 00:00:00

abstract：:To investigate the cyclic fatigue resistance of novel ROTATE instruments and to compare with the same size of One Curve, TF adaptive, and Mtwo instruments using a root canal simulation model. Four-type of 25/0.06 instruments were used: ROTATE, Mtwo, One Curve, TF adaptive. Instruments were divided into two subgroups: ...

journal_title：Microscopy research and technique

authors： Ertuğrul İF,Orhan EO

更新日期：2019-12-01 00:00:00

abstract：:The epididymal junctional complex between adjacent principal cells is composed of apically located gap, adherens and tight junctions. Tight junctions between adjacent epithelial cells lead to the formation of the blood-epididymal barrier. The objectives of this study were to examine the structure of the epididymal jun...

journal_title：Microscopy research and technique

authors： Cyr DG,Robaire B,Hermo L

更新日期：1995-01-01 00:00:00

abstract：:Vanda tessellata (Roxb.) Hook. ex G. Don. (grey orchid, family Orchidaceae) is an epiphytic orchid of horticultural importance and currently under threat due to overharvesting and habitat destruction. Micropropagation protocols were developed for the production of grey orchid but the survival success of in vitro regen...

journal_title：Microscopy research and technique

authors： Mani M,Rasangam L,Selvam P,Shekhawat MS

更新日期：2020-10-22 00:00:00

abstract：:The metrial gland develops in the uterus of many rodent species during normal pregnancy. It is a maternally-derived tissue that contains stromal and vascular elements plus a population of large cells, striking in their light microscopic appearance due to the presence of numerous cytoplasmic granules. These cells, whic...

journal_title：Microscopy research and technique

authors： Croy BA,Kiso Y

更新日期：1993-06-15 00:00:00