Abstract:
:We measured cell membrane deformations under the modulation of piconewton magnetic force by using optical tracking and noninterferometric widefield optical profilometry. The magnetic force was applied to fibronectin-coated paramagnetic beads that bound to transmembrane protein integrins. At an image-acquisition rate of 20 frame/min, optical tracking provided positioning accuracy better than 70 nm for bead displacements on cell membranes, and optical profilometry obtained membrane topography with 20 nm depth resolution. We elucidated the correlation between the bead movements and membrane deformations. When the magnetic force dominated the bead movements, the membrane arose in front of the bead and the height increased with the bead velocity. On the other hand, when the bead was mainly driven by the cytoskeletons, the membrane profiles showed no relevance to the motion of the bead. In this case, the bead moved faster on smooth membranes. A model based on the dynamics of actin cytoskeletons is proposed to explain these observation results.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Wang CC,Jian HJ,Wu CW,Lee CHdoi
10.1002/jemt.20593subject
Has Abstractpub_date
2008-08-01 00:00:00pages
594-8issue
8eissn
1059-910Xissn
1097-0029journal_volume
71pub_type
杂志文章abstract::The pollen morphology of 11 salt tolerant plant species of family Amaranthaceae from the salt range of Northern Punjab, Pakistan has been studied. The palyno-morphological characters were examined using light and scanning electron microscope. The examined all salt tolerant species have a slight difference in size but ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23173
更新日期:2019-03-01 00:00:00
abstract::In present study, multiple microscope techniques were used for the systematics identification of the species Asplenium dalhousiae. The plant was collected from different phytogeographical and its natural habitat of Pakistan, where it shows higher diversity. Morphology, foliar epidermal anatomy, and spore morphological...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23189
更新日期:2019-04-01 00:00:00
abstract::The purpose of this study was to evaluate mineral content of root canal dentin after treatment with different antibiotic pastes including the mixture of metronidazole, ciprofloxacin, doxycycline, cefaclor, amoxicillin, or minocycline. Fifty extracted maxillary canine teeth were randomly divided into five groups (n = 1...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23155
更新日期:2019-02-01 00:00:00
abstract::The ionic liquids (ILs) are recognized as the potential solvents for the pretreatment of lignocellulosic materials before biomass conversion. However, little knowledge of how the cell wall of biomass responds to the IL locally and dynamically during the pretreatment is available. In the current work, the process of IL...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22379
更新日期:2014-08-01 00:00:00
abstract::The mint beetle or green mint beetle, Chrysolina herbacea, is one of the most important insect pests of mint herb. These insects are associated with plants belonging to the Lamiaceae family, in particular the Mentha genus. They cause serious damages to plants by consuming their leaves. For this reason, it is an import...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23671
更新日期:2020-12-11 00:00:00
abstract::Transit from the nuclear complex to the cytoplasm through the nuclear pore complex permits modification of mRNA, including processing such as splicing, capping, and polyadenylation, etc. At each of these events, mRNA interacts with various proteins to form mRNA-protein complex. Visualizing the mRNA is crucial for unde...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23214
更新日期:2019-06-01 00:00:00
abstract::Virus particles and viral inclusions were detected by transmission electron microscopy examination of sections of the seminal vesicles and mucus gland of asymptomatic young drones from colonies of Apis mellifera lightly infested by Varroa mite. In the mucus gland the infection was found in the muscular sheath and epit...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22024
更新日期:2012-07-01 00:00:00
abstract::The flower buds of five Lonicera species, Lonicera japonica Thunb., L. macranthoides Hand.-Mazz., L. hypoglauca Miq., L. confusa DC. and L. fulvotomentosa Hsu et S.C. Cheng are confusable and usually utilized under the same name "Jinyinhua" in different areas for morphological similarity. Studies found that these five...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20882
更新日期:2011-02-01 00:00:00
abstract::All organisms exhibit significant daily rhythms in a myriad of functions from molecular levels to the level of the whole organism. Significantly, most of these rhythms will persist under constant conditions, showing that they are driven by an internal circadian clock. In birds the circadian system is composed of sever...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1068
更新日期:2001-04-01 00:00:00
abstract::Numerous highly fluorescent macrophages (MPhi), designated "smoker cells," exist in the lungs of smokers and subjects who have quit smoking within 5 years. The brightly fluorescent MPhi, however, are not present in the lungs of never smokers. Some investigators have speculated that the intense fluorescence of the MPhi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20191
更新日期:2005-06-01 00:00:00
abstract::Scanning electron microscope images of complete and damaged frustules of Nitzschia marina Grun. revealed an unusual chambered structure of the valve. This structure is not present in any existing nitzschioid genus and warrants an independent generic status for this taxon, which is therefore separated into Alveus gen. ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(199601)33:1<2::AID-JEMT2>3
更新日期:1996-01-01 00:00:00
abstract::The family Scrophulariaceae consists of taxonomically complex genera and species. The delimitation of the taxa within this family is always challenging. In this paper, we studied leaf epidermis anatomical characteristics and its taxonomic significance of four species belonging to four genera of the family Scrophularia...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23608
更新日期:2020-09-29 00:00:00
abstract::The Dominican Republic (DR) has experienced tremendous increase in the number of cases of dengue virus (DENV) reported in the past few years. There are four serotypes of DENV (1-4), and each can cause classic dengue fever (DF), dengue hemorrhagic fever (DHF), or dengue shock syndrome (DSS). DENV-1-4 are currently circ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20225
更新日期:2005-11-01 00:00:00
abstract::In this study, we aimed to increase the sensitivity of protein labeling using 1.4 nm gold nanoparticles and glutamate δ2 receptor (GluD2) from the postsynaptic membrane of the Purkinje cells. The very small marker size of the particles reduces the steric hindrance between antibodies leading to a higher labeling effici...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22043
更新日期:2012-09-01 00:00:00
abstract::Microscopic magnetic resonance imaging (µMRI) T2 data from canine cartilage at different tibial locations were analyzed to investigate the influences of spatial resolution and pixel position on the T2 sensitivity to osteoarthritis (OA). Five experimental factors were investigated: inaccurate pixel position, different ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22694
更新日期:2016-08-01 00:00:00
abstract::When 20-day-old rats are placed on a vitamin A deficient diet (VAD) for a period of 10 weeks, the seminiferous tubules are found to contain only Sertoli cells, a few residual A0, A1 spermatogonia, and preleptotene spermatocytes (PL). The type A1 spermatogonia and PL spermatocytes are arrested in their G2 phase. In VAD...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070200106
更新日期:1992-01-01 00:00:00
abstract::Nowadays the processes of crossing of different mammalian species are well characterized in light of genetics, and possibility of reproduction. Generally, lack detailed studies on changes in anatomy and histology of internal systems of hybrids. Bison bonasus hybrid is an interspecific species between the bull of Europ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23516
更新日期:2020-10-01 00:00:00
abstract::The present study investigated the regenerative potential of connective tissues harvested from two palatal areas widely used as donor sites for muco-gingival surgical approaches. Connective tissue grafts (CTGs) were obtained by de-epithelialisation of a free gingival graft (deCTG) and by a split flap approach from a p...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22925
更新日期:2017-12-01 00:00:00
abstract::Low voltage electron microscopes working in transmission mode, like LVEM5 (Delong Instruments, Czech Republic) working at accelerating voltage 5 kV or scanning electron microscope working in transmission mode with accelerating voltage below 1 kV, require ultrathin sections with the thickness below 20 nm. Decreasing of...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22659
更新日期:2016-06-01 00:00:00
abstract::Acrylic resin mixtures are commonly used to study microscopic sections of biological specimens, giving the advantage of good morphological preservation. Existing embedding protocols, however, are suitable for tissue blocks, not exceeding 1 mm in thickness. We have developed a protocol to embed larger specimens (up to ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20139
更新日期:2005-01-01 00:00:00
abstract::The mechanical properties of healing ligaments and tendons are not comparable to those of normal tissue. To improve the quality of the ligament healing, therapeutic strategies include gene transfer or placement of mesenchymal stem cells at the healing site. Studies show that marker genes, growth factors, and antisense...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10114
更新日期:2002-07-01 00:00:00
abstract::The present paper covers the results of different transmission electron microscopy studies on silver halide microcrystals. Pure AgBr as well as core-shell AgBr-AgBrI crystals are investigated. In the former parallel and non-parallel twinning modes yielding tabular and needle- or tetrahedral-shaped microcrystals, respe...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980715)42:2<85::AID-JEMT
更新日期:1998-07-15 00:00:00
abstract::This study evaluated the fluoride (F) release and remineralizing potential of varnishes containing sodium fluoride (5% NaF), 5% NaF with CPP-ACP and 5% NaF with TCP in early caries lesions in primary teeth. To determine the F release at 1, 4, 6, 24, 72, and 168 hr, strips were covered with the varnishes and immersed i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23662
更新日期:2020-12-05 00:00:00
abstract::In this paper, an application of contact microradiography with soft X-rays for detecting the uptake site of heavy metal in the whole plant leaves is investigated. The X-ray source is a laser-plasma one based on an Nd:glass laser. The soft X-ray radiation emitted from the plasma laser targets of magnesium, iron, and co...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20350
更新日期:2006-08-01 00:00:00
abstract::The presence of hard jaws (trophi), with species-specific shape and size, is a taxonomic feature of Rotifera, a group of microscopic metazoans. Since trophi are used to discriminate among species, it is important to know whether these structures change in taxonomically important ways during postembryonic development. ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20319
更新日期:2006-04-01 00:00:00
abstract::MIS, as a differentiate and antiproliferative agent, is precisely regulated, for example, at the transcriptional level by such transacting factors as SRY, and posttranslationally by testosterone. Processing of MIS most likely requires an as yet unknown in vivo protease which probably serves to control cleavage of MIS ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070250205
更新日期:1993-06-01 00:00:00
abstract::In the Oriental hornet Vespa orientalis (Hymenoptera, Vespinae), there is on the dorsal side of the thorax, beneath the mesoscutum plate of the prothorax and around the median notal suture, a lump that, in the course of hornet activity, is warmer by 9 degrees C from the surrounding milieu and by up to 6 degrees C from...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20257
更新日期:2005-12-15 00:00:00
abstract::Protein expression patterns are a primary determinant of tissue function and in this study we developed methods to study protein expression over macroscopic distances at subcellular levels of detail. Using the mammalian lens as a model tissue system, we show that by combining two-photon microscopy with novel image mon...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10360
更新日期:2003-09-01 00:00:00
abstract::Polarized microscopy provides unique information on anisotropic samples. In its most complete implementation, namely Mueller microscopy, this technique is well suited for the visualization of fibrillar proteins orientations, with collagen in the first place. However, the intrinsic optical anisotropy of unstained tissu...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22530
更新日期:2015-08-01 00:00:00
abstract::We present de novo studies and review published efforts from our laboratory, spanning 12 years (from 1988 to 2000), where we have used ultrastructural approaches to study the functional anatomy of the microvasculature in man and animals in health and disease. These efforts have defined a new endothelial cell organelle...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10087
更新日期:2002-06-01 00:00:00