Abstract:
:In this study, we aimed to increase the sensitivity of protein labeling using 1.4 nm gold nanoparticles and glutamate δ2 receptor (GluD2) from the postsynaptic membrane of the Purkinje cells. The very small marker size of the particles reduces the steric hindrance between antibodies leading to a higher labeling efficiency of more than one subunit per single receptor molecule. The nanoparticles are visible in 200 kV dark-field scanning transmission electron microscope on freeze-fractured carbon replica of nervous tissue after plasma cleaning treatment. The different elemental composition of nanoparticles as Au nanogold or CdS quantum dot can be distinguished by energy dispersive X-ray spectroscopy. This method ensures detection of an average of three subunits per GluD2 and often labels all four of them with 1.4 nm Au nanoparticles. It is concluded that this high-resolution microscopic method is useful for exploring the quaternary structure of membrane proteins.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Loukanov AR,Gagov Hdoi
10.1002/jemt.22043subject
Has Abstractpub_date
2012-09-01 00:00:00pages
1159-64issue
9eissn
1059-910Xissn
1097-0029journal_volume
75pub_type
杂志文章abstract::The epididymal junctional complex between adjacent principal cells is composed of apically located gap, adherens and tight junctions. Tight junctions between adjacent epithelial cells lead to the formation of the blood-epididymal barrier. The objectives of this study were to examine the structure of the epididymal jun...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070300105
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abstract::Sequences highly similar (>95%) to the mouse mammary tumor virus (MMTV) env gene have been amplified from human DNA samples, including DNA samples from patients with breast cancer (BC) and persons who did not have BC. The sequences from human DNA were distinct from the MMTV sequences used as controls in these PCR reac...
journal_title:Microscopy research and technique
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abstract::Although the Geographical Information System (GIS), which integrates computerized drawing computer assisted design (CAD) and relational databases (data base management system (DBMS)), is best known for applications in geographical and planning cartography, it can also use many kinds of information concerning the terri...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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abstract::This was the first comprehensive pollen micromorphological investigation of lactiferous flora (Apocynaceae) of District Rawalpindi, Pakistan. The pollen morphology of 10 species of the family Apocynaceae was observed and documented using light microscopy and scanning electron microscopy. Pollen was found subspheroidal...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23293
更新日期:2019-09-01 00:00:00
abstract::The fine structure of the taste buds of circumvallate papillae of two strains of mice was studied by electron microscopy. Mice anesthetized with ketamine were perfused through the heart with a double aldehyde mixture in cacodylate buffer and the tissues embedded in Epon. Semi-serial sections were employed. The morphol...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070260304
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abstract::We compared physiological and ultrastructural indices of acute lung injury (ALI) during septic shock caused by taxonomically diverse pathogens to distinguish ALI due to endogenous inflammatory mediators vs. microbial exotoxins or other factors. Conscious rats were infected i.v. with gram-negative Escherichia coli (EC,...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070260512
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abstract::Lungfish are a group of ancient fish, represented almost continuously in the fossil record from their first appearance in the Devonian to the present time. They have numerous unique characters in the dentition, found only among lungfish, as well as some that are shared with other groups of fish and with higher vertebr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(199601)33:1<2::AID-JEMT2>3
更新日期:1996-01-01 00:00:00
abstract::We have summarized current knowledge on the aging of the immune system in three vertebrate groups: fish, amphibians and birds. Available data are few due to difficulties in studying ageing in natural populations and in accurately determining age. In all vertebrates, the most obvious evidence of the senescence of lymph...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19970301)36:5<382::AID-JEM
更新日期:1997-03-01 00:00:00
abstract::The pollen morphology of 11 salt tolerant plant species of family Amaranthaceae from the salt range of Northern Punjab, Pakistan has been studied. The palyno-morphological characters were examined using light and scanning electron microscope. The examined all salt tolerant species have a slight difference in size but ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23173
更新日期:2019-03-01 00:00:00
abstract::Malaria is a serious worldwide disease, caused by a bite of a female Anopheles mosquito. The parasite transferred into complex life round in which it is grown and reproduces into the human body. The detection and recognition of Plasmodium species are possible and efficient through a process called staining (Giemsa). T...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23269
更新日期:2019-07-01 00:00:00
abstract::Ascidian embryos are useful for examining how events that occur during fertilization and cleavage affect gastrulation because they gastrulate early in development, during the seventh cleavage. In ascidians, both dorsal-ventral and anterior-posterior axes are determined before first cleavage. The dorsal-ventral axis is...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070260403
更新日期:1993-11-01 00:00:00
abstract::This present study is the first report on the detailed foliar epidermal anatomy and micromorphology of seeds of five species of Vitaceae from Pakistan using light microscopy (LM) and scanning electron microscopy (SEM). The studied species occur at various localities particularly higher altitude. Qualitative and quanti...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23102
更新日期:2019-04-01 00:00:00
abstract::Gymnosporia arenicola Jordaan (Celastraceae) is a shrub or small tree, which naturally occurs in coastal sand dunes of Southern Mozambique and South Africa. Its dried leaf is often used in traditional medicine for the treatment of infectious and inflammatory diseases. Hereby, we present results of studies carried out ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22565
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abstract::Microscopic imaging of uneven surfaces is difficult because of the limited depth of field. In this study, we developed a rapid auto-focus method for uneven surfaces based on image fusion. The Prewitt operator was used to detect the vertical edges of the images. Then, the focus position was theoretically calculated usi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23328
更新日期:2019-09-01 00:00:00
abstract::To investigate the cyclic fatigue resistance of novel ROTATE instruments and to compare with the same size of One Curve, TF adaptive, and Mtwo instruments using a root canal simulation model. Four-type of 25/0.06 instruments were used: ROTATE, Mtwo, One Curve, TF adaptive. Instruments were divided into two subgroups: ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23374
更新日期:2019-12-01 00:00:00
abstract::Effects of microstructure and phase component on mechanical property of spearer propodus of mantis shrimp were investigated. The spearer propodus consisted of three layers including epicuticle (outer layer), exocuticle (middle layer), and endocuticle (inner layer). The outer layer was composed of fluorapatite, which w...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23598
更新日期:2020-09-16 00:00:00
abstract::The fixed number years-identification of Chinese material medicinal was a difficulty in the process of the traditional Chinese pharmacology, the phenomenon was found in our study of telomerase that the speed of the vascular cambium outside expanding growth fluctuated in a definite value. Based on it, we put forward a ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
abstract::Linepithema micans Forel is a poorly studied species that is now considered a pest in vineyards in South Brazil. Larval characters have been used in a few phylogenetic studies, and their importance greatly depends in the amount of available information on different species. This study presents a complete panorama on t...
journal_title:Microscopy research and technique
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abstract::In order to understand the interaction between dendritic cells (DCs) and helper T (Th) cells in the region exposed to antigens during pulmonary delayed-type hypersensitivity (DTH), which is considered to be mediated by Th1 cells, we immunohistochemically investigated their spatial relationship in the cellular infiltra...
journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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更新日期:2013-03-01 00:00:00
abstract::Cell-free extracts of Xenopus eggs were used to investigate how differing lamin sub-types are incorporated into a lamina and how lamina composition influences DNA replication. Purified recombinant human lamin A (HlaminA) was inoculated into egg extracts, which support in vitro nuclear assembly. The route of incorporat...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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abstract::We report an ingenious method of super-resolution optical microscopy utilizing scannable cantilever-combined microsphere. By scanning the microsphere over the sample surface in a cantilever-combined microsphere-sample contact state, super-resolution images can be acquired at arbitrary sample regions through near-field...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070230104
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abstract::Voltage-gated sodium channels are largely localized to the nodes of Ranvier in myelinated axons, providing the physiological basis for saltatory conduction. Studies using antisodium channel antibodies have shown that along demyelinated axons sodium channels form new distributions. The nature of this changed distributi...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
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更新日期:1996-08-01 00:00:00
abstract::We present a semiautomated software solution to the problem of extending the lateral field of view of a classical microscope. The initial requirements are a set of overlapping images, along with their user-provided coarse mosaic. Our solution then refines this initial mosaic in a fully automatic fashion. We rely on a ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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abstract::This work investigates recording bits on recordable and rewritable blu-ray discs using atomic force microscopy and conducting atomic force microscopy with high contrast, respectively. The geometric structure of the recording bits is clearly observed in images, which, when coupled with cross-section analysis, yields pr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20745
更新日期:2010-01-01 00:00:00